scholarly journals An in vitro model for cytogenetic conversion in CML. Interferon-alpha preferentially inhibits the outgrowth of malignant stem cells preserved in long-term culture.

1998 ◽  
Vol 102 (5) ◽  
pp. 976-983 ◽  
Author(s):  
J J Cornelissen ◽  
R E Ploemacher ◽  
B W Wognum ◽  
A Borsboom ◽  
H C Kluin-Nelemans ◽  
...  
Keyword(s):  
Stem Cells ◽  
Interferon Alpha ◽  
In Vitro Model ◽  
Long Term Culture ◽  
Vitro Model

Electrospun nanofibers facilitate better alignment, differentiation, and long-term culture in an in vitro model of the neuromuscular junction (NMJ)

2018 ◽  
Vol 6 (12) ◽  
pp. 3262-3272 ◽  
Author(s):  
Baiwen Luo ◽  
Lingling Tian ◽  
Nuan Chen ◽  
Seeram Ramakrishna ◽  
Nitish Thakor ◽  
...  
Keyword(s):  
Neuromuscular Junction ◽  
Culture System ◽  
In Vitro Model ◽  
Electrospun Nanofibers ◽  
Nanofibrous Scaffold ◽  
Long Term Culture ◽  
Vitro Model

An electrospun nanofibrous scaffold is used as a novel in vitro culture system to provide long-term support for NMJ formation.

Immunophenotyping of bone marrow-derived mesenchymal stem cells after transplantation in a heterologous in-vitro model using Laser Scanning Cytometry

2004 ◽  
Vol 52 (S 1) ◽  
Author(s):  
J Garbade ◽  
D Lenz ◽  
A Rastan ◽  
MJ Barten ◽  
A Tarnok ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Laser Scanning ◽  
In Vitro Model ◽  
Laser Scanning Cytometry ◽  
Vitro Model

A scaffold-free in vitro model for osteogenesis of human mesenchymal stem cells

2011 ◽  
Vol 43 (2) ◽  
pp. 91-100 ◽  
Author(s):  
Cornelia Hildebrandt ◽  
Heiko Büth ◽  
Hagen Thielecke
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vitro Model ◽  
Human Mesenchymal Stem Cells ◽  
Vitro Model

scholarly journals Microcavity arrays as an in vitro model system of the bone marrow niche for hematopoietic stem cells

2016 ◽  
Vol 364 (3) ◽  
pp. 573-584 ◽  
Author(s):  
Patrick Wuchter ◽  
Rainer Saffrich ◽  
Stefan Giselbrecht ◽  
Cordula Nies ◽  
Hanna Lorig ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Hematopoietic Stem Cells ◽  
In Vitro Model ◽  
Model System ◽  
Bone Marrow Niche ◽  
Hematopoietic Stem ◽  
In Vitro Model System ◽  
Vitro Model

Features of the in vitro expression profile of hippocampal neurogenic niche cells during optogenetic stimulation

2021 ◽  
Vol 67 (1) ◽  
pp. 34-41
Author(s):  
E.D. Khilazheva ◽  
A.V. Morgun ◽  
E.B. Boytsova ◽  
A.I. Mosiagina ◽  
A.N. Shuvaev ◽  
...  
Keyword(s):  
Stem Cells ◽  
Expression Profile ◽  
In Vitro Model ◽  
Postnatal Period ◽  
Proliferating Cells ◽  
Aberrant Expression ◽  
Intercellular Interactions ◽  
Neurogenic Niche ◽  
Vitro Model

In the central nervous system of mammals, there are specialized areas in which neurogenesis — neurogenic niches — is observed in the postnatal period. It is believed that astrocytes in the composition of neurogenic niches play a significant role in the regulation of neurogenesis, and therefore they are considered as a promising “target” for the possible control of neurogenesis, including the use of optogenetics. In the framework of this work, we formed an in vitro model of a neurogenic niche, consisting of cerebral endothelial cells, astrocytes and neurospheres. Astrocytes in the neurogenic niche model expressed canalorodopsin ChR2 and underwent photoactivation. The effect of photoactivated astrocytes on the expression profile of neurogenic niche cells was evaluated using immunocytochemical analysis methods. It was found that intact astrocytes in the composition of the neurogenic niche contribute to neuronal differentiation of stem cells, as well as the activation of astroglia expressing photosensitive proteins, changes the expression of molecules characterized by intercellular interactions of pools of resting and proliferating cells in the composition of the neurogenic niche with the participation of NAD+ (Cx43, CD38, CD157), lactate (MCT1). In particular, the registered changes reflect a violation of the paracrine intercellular interactions of two subpopulations of cells, one of which acts as a source of NAD+, and the second as a consumer of NAD+ to ensure the processes of intracellular signal transduction; a change in the mechanisms of lactate transport due to aberrant expression of the lactate transporter MCT1 in cells forming a pool of cells developing along the neuronal path of differentiation. In general, with photostimulation of niche astrocytes, the total proliferative activity increases mainly due to neural progenitor cells, but not neural stem cells. Thus, optogenetic activation of astrocytes can become a promising tool for controlling the activity of neurogenesis processes and the formation of a local proneurogenic microenvironment in an in vitro model of a neurogenic niche.

Cortical Neurons Derived from Equine Induced Pluripotent Stem Cells Are Susceptible to Neurotropic Flavivirus Infection and Replication: An In Vitro Model for Equine Neuropathic Diseases

2018 ◽  
Vol 27 (10) ◽  
pp. 704-715 ◽  
Author(s):  
Patrick R.J. Fortuna ◽  
Helle Bielefeldt-Ohmann ◽  
Dmitry A. Ovchinnikov ◽  
Ernst J. Wolvetang ◽  
Deanne J. Whitworth
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Cortical Neurons ◽  
In Vitro Model ◽  
Vitro Model ◽  
Induced Pluripotent ◽  
Flavivirus Infection
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