scholarly journals Microcavity arrays as an in vitro model system of the bone marrow niche for hematopoietic stem cells

2016 ◽  
Vol 364 (3) ◽  
pp. 573-584 ◽  
Author(s):  
Patrick Wuchter ◽  
Rainer Saffrich ◽  
Stefan Giselbrecht ◽  
Cordula Nies ◽  
Hanna Lorig ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Hematopoietic Stem Cells ◽  
In Vitro Model ◽  
Model System ◽  
Bone Marrow Niche ◽  
Hematopoietic Stem ◽  
In Vitro Model System ◽  
Vitro Model

An In Vitro Model for Grafting of Hematopoietic Stem Cells Predicts Bone Marrow Reconstitution of Myeloablative Mice

2003 ◽  
Vol 12 (2) ◽  
pp. 243-252 ◽  
Author(s):  
T. Madhusudhan ◽  
Anshu Richhariya ◽  
Subeer S. Majumdar ◽  
Asok Mukhopadhyay
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Hematopoietic Stem Cells ◽  
In Vitro Model ◽  
Hematopoietic Stem ◽  
Vitro Model

scholarly journals Molecular Modulation of Fetal Liver Hematopoietic Stem Cell Mobilization into Fetal Bone Marrow in Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Huihong Zeng ◽  
Jiaoqi Cheng ◽  
Ying Fan ◽  
Yingying Luan ◽  
Juan Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Hematopoietic Stem Cells ◽  
Vascular Endothelial Cells ◽  
Fetal Liver ◽  
Bone Marrow Niche ◽  
Self Renewal ◽  
Hematopoietic Stem ◽  
Fetal Bone

Development of hematopoietic stem cells is a complex process, which has been extensively investigated. Hematopoietic stem cells (HSCs) in mouse fetal liver are highly expanded to prepare for mobilization of HSCs into the fetal bone marrow. It is not completely known how the fetal liver niche regulates HSC expansion without loss of self-renewal ability. We reviewed current progress about the effects of fetal liver niche, chemokine, cytokine, and signaling pathways on HSC self-renewal, proliferation, and expansion. We discussed the molecular regulations of fetal HSC expansion in mouse and zebrafish. It is also unknown how HSCs from the fetal liver mobilize, circulate, and reside into the fetal bone marrow niche. We reviewed how extrinsic and intrinsic factors regulate mobilization of fetal liver HSCs into the fetal bone marrow, which provides tools to improve HSC engraftment efficiency during HSC transplantation. Understanding the regulation of fetal liver HSC mobilization into the fetal bone marrow will help us to design proper clinical therapeutic protocol for disease treatment like leukemia during pregnancy. We prospect that fetal cells, including hepatocytes and endothelial and hematopoietic cells, might regulate fetal liver HSC expansion. Components from vascular endothelial cells and bones might also modulate the lodging of fetal liver HSCs into the bone marrow. The current review holds great potential to deeply understand the molecular regulations of HSCs in the fetal liver and bone marrow in mammals, which will be helpful to efficiently expand HSCs in vitro.

scholarly journals Anaerobic Co-Culture of Mesenchymal Stem Cells and Anaerobic Pathogens - A New In Vitro Model System

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e78226 ◽  
Author(s):  
Katja Kriebel ◽  
Anne Biedermann ◽  
Bernd Kreikemeyer ◽  
Hermann Lang
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vitro Model ◽  
Model System ◽  
In Vitro Model System ◽  
Vitro Model

A novel 3D prostate cancer bone marrow metastasis in vitro model system

2015 ◽  
Vol 43 (9) ◽  
pp. S60
Author(s):  
Eman Mohamed Othman Mosaad ◽  
Karen Chambers ◽  
Judith Clements ◽  
Michael R. Doran
Keyword(s):  
Prostate Cancer ◽  
Bone Marrow ◽  
In Vitro Model ◽  
Model System ◽  
Bone Marrow Metastasis ◽  
In Vitro Model System ◽  
Vitro Model

scholarly journals Site-1 protease ablation in the osterix-lineage in mice results in bone marrow neutrophilia and hematopoietic stem cell alterations

Biology Open ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. bio052993
Author(s):  
Debabrata Patra ◽  
Joongho Kim ◽  
Qiang Zhang ◽  
Eric Tycksen ◽  
Linda J. Sandell
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Hematopoietic Stem Cells ◽  
Bone Development ◽  
Adipogenic Differentiation ◽  
Bone Marrow Niche ◽  
Hematopoietic Stem ◽  
Protein Mass ◽  
Cell Alterations

ABSTRACTSite-1 protease (S1P) ablation in the osterix-lineage in mice drastically reduces bone development and downregulates bone marrow-derived skeletal stem cells. Here we show that these mice also suffer from spina bifida occulta with a characteristic lack of bone fusion in the posterior neural arches. Molecular analysis of bone marrow-derived non-red blood cell cells, via single-cell RNA-Seq and protein mass spectrometry, demonstrate that these mice have a much-altered bone marrow with a significant increase in neutrophils and Ly6C-expressing leukocytes. The molecular composition of bone marrow neutrophils is also different as they express more and additional members of the stefin A (Stfa) family of proteins. In vitro, recombinant Stfa1 and Stfa2 proteins have the ability to drastically inhibit osteogenic differentiation of bone marrow stromal cells, with no effect on adipogenic differentiation. FACS analysis of hematopoietic stem cells show that despite a decrease in hematopoietic stem cells, S1P ablation results in an increased production of granulocyte-macrophage progenitors, the precursors to neutrophils. These observations indicate that S1P has a role in the lineage specification of hematopoietic stem cells and/or their progenitors for development of a normal hematopoietic niche. Our study designates a fundamental requirement of S1P for maintaining a balanced regenerative capacity of the bone marrow niche.

scholarly journals ATM-deficient human neural stem cells as an in vitro model system to study neurodegeneration

DNA Repair ◽  
2013 ◽  
Vol 12 (8) ◽  
pp. 605-611 ◽  
Author(s):  
Luigi Carlessi ◽  
Elena Fusar Poli ◽  
Lidia De Filippis ◽  
Domenico Delia
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
In Vitro Model ◽  
Model System ◽  
Human Neural Stem Cells ◽  
In Vitro Model System ◽  
Vitro Model

scholarly journals Promise(s) of Mesenchymal Stem Cells as an In Vitro Model System to Depict Pre-Diabetic/Diabetic Milieu in WNIN/GR-Ob Mutant Rats

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e48061 ◽  
Author(s):  
Soundarya L. Madhira ◽  
Satya S. Challa ◽  
Maniprabha Chalasani ◽  
Giridharan Nappanveethl ◽  
Ramesh R. Bhonde ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vitro Model ◽  
Model System ◽  
In Vitro Model System ◽  
Vitro Model
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