scholarly journals Evaluation of Bionike One-Step Tests for the Detection of Drugs of Abuse in Urine

1997 ◽  
Vol 34 (1) ◽  
pp. 74-80 ◽  
Author(s):  
E R S Brown ◽  
D R Jarvie ◽  
D Simpson
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Drugs Of Abuse ◽  
Urine Samples ◽  
Gas Chromatography Mass Spectrometry ◽  
Site Testing ◽  
Immunoassay Technique ◽  
Or Groups ◽  
One Step ◽  
Good Agreement

Bionike one-step tests were used to screen urine samples for amphetamines, methamphetamines, benzodiazepines, cannabinoids, methadone and opiates, and results were compared with those obtained using enzyme multiplied immunoassay technique d.a.u. assays. Taking into consideration different threshold levels and possible differences in cross-reactivities, there was good agreement between the methods. Results of Bionike tests correlated well with amphetamines, methadone and opiates detected in urine using gas chromatography—mass spectrometry. Bionike methods are rapid, simple to use, and relatively inexpensive for on-site testing of individual drugs or groups of drugs in urine.

THC and CBD concentrations in blood, oral fluid and urine following a single and repeated administration of “light cannabis”

2020 ◽  
Vol 58 (5) ◽  
pp. 682-689 ◽  
Author(s):  
Roberta Pacifici ◽  
Simona Pichini ◽  
Manuela Pellegrini ◽  
Maria Concetta Rotolo ◽  
Raffaele Giorgetti ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Oral Fluid ◽  
Repeated Administration ◽  
Urine Samples ◽  
Gas Chromatography Mass Spectrometry ◽  
Sample Collection ◽  
Valuable Alternative ◽  
The Mean ◽  
Single And Repeated Administration

AbstractBackground“Light cannabis” is a product legally sold in Europe with Δ9-tetrahydrocannabinol (THC) concentration lower than 0.2% and variable cannabidiol (CBD) content. We studied THC and CBD excretion profiles in blood, oral fluid (OF) and urine after smoking one or four light cannabis cigarettes.MethodsBlood, OF and urine samples were obtained from six healthy light cannabis consumers after smoking one 1 g cigarette containing 0.16% THC and 5.8% CBD and from six others after smoking four 1 g cigarettes within 4 h. Sample collection began 0.5 and 4.5 h after smoking one or four cigarettes, respectively. Cannabinoid concentrations were quantified by gas chromatography-mass spectrometry (GC-MS).ResultsAt the first collection, the highest THC and CBD concentrations occurred in blood (THC 7.0–10.8 ng/mL; CBD 30.2–56.1 ng/mL) and OF (THC 5.1–15.5 ng/mL; CBD 14.2–28.1 ng/mL); similar results occurred 0.5 h after the last of four cigarettes in blood (THC 14.1–18.2 ng/mL, and CBD 25.6–45.4 ng/mL) and OF (THC 11.2–24.3 ng/mL; CBD 14.4–37.0 ng/mL). The mean OF to blood ratio ranged from 0.6 to 1.2 after one and 0.6 to 1.9 after four light cannabis cigarettes. THC/CBD ratios in blood and OF were never greater than 2. Urinary 11-nor-9-carboxy-THC concentrations peaked 8 h after one and four cigarettes.ConclusionsOF was a valuable alternative to blood in monitoring consumption of light cannabis. Blood and OF THC/CBD concentration ratios, never exceeded 2, possibly providing a useful biomarker to identify light cannabis vs illegal higher THC cannabis use, where THC/CBD ratios are generally greater than 10.

scholarly journals Methylmalonic acid quantification by stable isotope dilution gas chromatography-mass spectrometry from filter paper urine samples

1996 ◽  
Vol 42 (6) ◽  
pp. 910-914 ◽  
Author(s):  
M T McCann ◽  
M M Thompson ◽  
I C Gueron ◽  
B Lemieux ◽  
R Giguère ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Stable Isotope ◽  
Isotope Dilution ◽  
Filter Paper ◽  
Methylmalonic Acid ◽  
Urine Samples ◽  
Gas Chromatography Mass Spectrometry ◽  
Urinary Creatinine ◽  
Stable Isotope Dilution

Abstract A specific method for quantification of methylmalonic acid (MMA) from urine samples dried onto filter paper is described. The method involves stable isotope dilution gas chromatography-mass spectrometry with [methyl-2H3]-MMA as the internal standard. MMA is stable in dry paper samples stored at room temperature for at least 2 weeks. The extraction efficiency of MMA from paper was 56-58%. The concentration of urinary MMA in dried filter paper specimens from 190 normal controls was 1.21 +/- 1.34 (mean +/- SD) mmol/mol of urinary creatinine. Age-related reference values are also reported. The concentrations, normalized to the urinary creatinine concentration, decrease with age. The applicability of this method to rapid screening for cobalamin (vitamin B12)-related disorders and methylmalonic aciduria is demonstrated.

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