Proceedings of the 2018 National Toxicology Program Satellite Symposium

The 2018 annual National Toxicology Program Satellite Symposium, entitled “Pathology Potpourri,” was held in Indianapolis, Indiana, at the Society of Toxicologic Pathology’s 37th annual meeting. The goal of this symposium was to present and discuss challenging diagnostic pathology and/or nomenclature issues. This article presents summaries of the speakers’ talks along with select images that were used by the audience for voting and discussion. Various lesions and other topics covered during the symposium included seminiferous tubule dysgenesis in rats, ameloblast and odontoblast degeneration/necrosis in a Sprague Dawley rat, intestinal leiomyositis in a beagle dog, gallbladder mucinous hyperplasia, focus of hepatocellular alteration and bile duct alteration in otters, renal tubule cytoplasmic vacuolation with basophilic granules in mice treated swith antisense oligonucleotide therapy, a uterine choriocarcinoma in a rhesus macaque, and rete ovarii proliferative ovarian lesions in various aged rat strains. One particularly provocative lesion was a malignant neoplastic proliferation in the renal pelvic region of a cynomolgus macaque from a 21-day study. Additional challenging lesions included thyroid proliferative lesions in zebra fish and gross findings in fish larvae during routine chemical screening. The Rabbit and Minipig International Harmonization of Nomenclature and Diagnostic Criteria Organ Working Groups also presented a series of challenging lesions.

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Proceedings of the 2014 National Toxicology Program Satellite Symposium

Toxicologic Pathology ◽

10.1177/0192623314555526 ◽

2014 ◽

Vol 43 (1) ◽

pp. 10-40 ◽

Cited By ~ 9

Author(s):

Susan A. Elmore ◽

Michelle C. Cora ◽

Margarita M. Gruebbel ◽

Schantel A. Hayes ◽

Jessica S. Hoane ◽

...

Keyword(s):

Satellite Symposium ◽

National Toxicology Program

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Proceedings of the 2012 National Toxicology Program Satellite Symposium

Toxicologic Pathology ◽

10.1177/0192623312467102 ◽

2012 ◽

Vol 41 (2) ◽

pp. 151-180 ◽

Cited By ~ 9

Author(s):

Susan A. Elmore ◽

Brian R. Berridge ◽

Michael C. Boyle ◽

Michelle C. Cora ◽

Mark J. Hoenerhoff ◽

...

Keyword(s):

Satellite Symposium ◽

National Toxicology Program

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Proceedings of the 2019 National Toxicology Program Satellite Symposium

Toxicologic Pathology ◽

10.1177/0192623319876929 ◽

2019 ◽

Vol 47 (8) ◽

pp. 913-953 ◽

Cited By ~ 1

Author(s):

Susan A. Elmore ◽

Mark F. Cesta ◽

Torrie A. Crabbs ◽

Kyathanahalli S. Janardhan ◽

Gregory A. Krane ◽

...

Keyword(s):

North Carolina ◽

Renal Tubule ◽

Rete Testis ◽

Similar Response ◽

Satellite Symposium ◽

Beagle Dogs ◽

Rat Strains ◽

National Toxicology Program ◽

Retinal Lesion ◽

Liver And Pancreas

The 2019 annual National Toxicology Program Satellite Symposium, entitled “Pathology Potpourri,” was held in Raleigh, North Carolina, at the Society of Toxicologic Pathology’s 38th annual meeting. The goal of this symposium was to present and discuss challenging diagnostic pathology and/or nomenclature issues. This article presents summaries of the speakers’ talks along with select images that were used by the audience for voting and discussion. Various lesions and topics covered during the symposium included aging mouse lesions from various strains, as well as the following lesions from various rat strains: rete testis sperm granuloma/fibrosis, ovarian cystadenocarcinoma, retro-orbital schwannoma, periductal cholangiofibrosis of the liver and pancreas, pars distalis hypertrophy, chronic progressive nephropathy, and renal tubule regeneration. Other cases included polyovular follicles in young beagle dogs and a fungal blood smear contaminant. One series of cases challenged the audience to consider how immunohistochemistry may improve the diagnosis of some tumors. Interesting retinal lesions from a rhesus macaque emphasized the difficulty in determining the etiology of any particular retinal lesion due to the retina’s similar response to vascular injury. Finally, a series of lesions from the International Harmonization of Nomenclature and Diagnostic Criteria Non-Rodent Fish Working Group were presented.

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Proceedings of the 2016 National Toxicology Program Satellite Symposium

Toxicologic Pathology ◽

10.1177/0192623316672074 ◽

2016 ◽

Vol 45 (1) ◽

pp. 11-51 ◽

Cited By ~ 5

Author(s):

Susan A. Elmore ◽

Vivian S. Chen ◽

Schantel Hayes-Bouknight ◽

Jessica S. Hoane ◽

Kyathanahalli Janardhan ◽

...

Keyword(s):

Malignant Glioma ◽

Granulomatous Inflammation ◽

Satellite Symposium ◽

Embryonic Mouse ◽

Mixed Glioma ◽

National Toxicology Program ◽

Rodent Brain ◽

Malignant Plasma Cell ◽

Rat Hearts ◽

Maturation Defect

The 2016 annual National Toxicology Program Satellite Symposium, entitled “Pathology Potpourri” was held in San Diego, CA, at the Society of Toxicologic Pathology’s (STP) 35th annual meeting. The goal of this symposium was to present and discuss challenging diagnostic pathology and/or nomenclature issues. This article presents summaries of the speakers’ talks, along with select images that were used by the audience for voting and discussion. Some lesions and topics covered during the symposium included malignant glioma and histiocytic sarcoma in the rodent brain; a new statistical method designed for histopathology data evaluation; uterine stromal/glandular polyp in a rat; malignant plasma cell tumor in a mouse brain; Schwann cell proliferative lesions in rat hearts; axillary schwannoma in a cat; necrosis and granulomatous inflammation in a rat brain; adenoma/carcinoma in a rat adrenal gland; hepatocyte maturation defect and liver/spleen hematopoietic defects in an embryonic mouse; distinguishing malignant glioma, malignant mixed glioma, and malignant oligodendroglioma in the rat; comparison of mammary gland whole mounts and histopathology from mice; and discussion of the International Harmonization of Nomenclature and Diagnostic Criteria collaborations.

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Incidences of Selected Lesions in Control Female Harlan Sprague–Dawley Rats from Two-Year Studies Performed by the National Toxicology Program

Toxicologic Pathology ◽

10.1080/01926230590961836 ◽

2005 ◽

Vol 33 (4) ◽

pp. 477-483 ◽

Cited By ~ 42

Author(s):

Amy E. Brix ◽

Abraham Nyska ◽

Joseph K. Haseman ◽

Donald M. Sells ◽

Micheal P. Jokinen ◽

...

Keyword(s):

Sprague Dawley ◽

Control Female ◽

National Toxicology Program ◽

Sprague Dawley Rats

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The Effect of Different Thyroxine Hormone (T4) Concentration on The Growth, Survival, and Pigment Development of Pink Zebra Fish Larvae (Brachydanio reiro)

Omni-Akuatika ◽

10.20884/1.oa.2018.14.2.538 ◽

2018 ◽

Vol 14 (2) ◽

Author(s):

Arifianto Heraedi ◽

Slamet Budi Prayitno ◽

Tristiana Yuniarti

Keyword(s):

Growth Rate ◽

Survival Rate ◽

Specific Growth Rate ◽

Specific Growth ◽

Fish Larvae ◽

Average Length ◽

Average Weight ◽

Zebra Fish ◽

Total Length ◽

Absolute Growth

The thyroxin hormone plays an important role in the process of metabolism, yolk sac absorbsion, and growth of fish. The aims of this research were to observe the effect of various concentration of thyroxin (T4) on the absolute growth weight, total length, survival rate, and pigment development of pink zebra fish larvae (Brachydanio reiro ) after being reared 42 days. The pink zebra fish larvae at 4 day age with the average weight of 0,002 – 0,003g and average length of ± 3.10 – 3.43 mm were immersed at various concentration of T4 for 24 hours. Prior the treatment fish larvae were dipped into 1 ppt salinity for 2 minutes then transferred into 1 Liter, 1ppt saline and various T4 in plastic bags. The thyroxin concentrations were A (0 mg / L); B (0.05 mg / L); C (0.10 mg / L) and D (0.15 mg / L) respectively. The stocking density was 40 fish/L. After that they were transferred into aquariums and reared for 42 days. Completely Randomized Design (RAL) with four treatments and three replications were used. The variables observed were absolute and specific growth, total length, survival rate and hue degree. The results showed that the thyroxin hormone had significant effect on absolute growth weight, total length and specific growth rate. The dosage 0.1 mg/L was the best treatment on absolute growth, total length, and specific growth rate. Whilee the survival rate showed no significant differences across the treatments. Treatment C also demonstrated the best ppigment development (14.60 + 0.36º hue) compared to others.

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Proceedings of the 2011 National Toxicology Program Satellite Symposium

Toxicologic Pathology ◽

10.1177/0192623311427713 ◽

2011 ◽

Vol 40 (2) ◽

pp. 321-344 ◽

Cited By ~ 12

Author(s):

Gary Boorman ◽

Torrie A. Crabbs ◽

Holly Kolenda-Roberts ◽

Ken Latimer ◽

Andrew D. Miller ◽

...

Keyword(s):

Satellite Symposium ◽

National Toxicology Program

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Comparative Incidences and Biological Outcomes for Thymoma in Various Rat Strains in National Toxicology Program Studies

Toxicologic Pathology ◽

10.1177/0192623319863119 ◽

2019 ◽

Vol 47 (7) ◽

pp. 833-841

Author(s):

Rebecca R. Moore ◽

Hiroaki Nagai ◽

Rodney A. Miller ◽

Jerry F. Hardisty ◽

Neil Allison ◽

...

Keyword(s):

Cause Of Death ◽

Early Mortality ◽

Sprague Dawley ◽

Rat Strains ◽

Fischer 344 ◽

National Toxicology Program ◽

Sd Rats ◽

Biological Outcomes ◽

Long Term Studies

Thymomas from 277 Fischer 344/N (F344/N), 10 Sprague Dawley (HSD:Sprague Dawley SD) (SD), 129 Wistar Han [Crl:WI(Han)] (WH), and 4 Wistar Outbred (WO) rats were reviewed from long-term studies in the National Toxicology Program (NTP) database. The incidence of thymomas in F344/N rats was slightly higher in males than in females, while the incidences in SD and WH rats were higher in females than in males. Only male WO rats were used in NTP studies. Of the 277 thymomas in F344/N rats, 235 (84.8%) were benign and 42 (15.2%) malignant, 14 of which exhibited metastasis. Of the 10 thymomas in SD rats, 5 (50%) were benign and 5 (50%) were malignant, one of which exhibited metastasis. Of the 129 thymomas in WH rats, 126 (98%) were benign and 3 (2%) were malignant, 1 with metastasis. Of the 4 thymomas in WO rats, 3 (75%) were benign and 1 (25%) was malignant, with no metastases. Malignant thymomas in F344/N and WH rats showed a propensity to be the cause of death and to result in early mortality, whereas the benign thymomas were associated less often with decreased survival. No occurrences of this neoplasm were reported to be related to exposure to any test articles.

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Effect of the antiglucocorticoid RU486 on adrenal steroidogenic enzyme activity and steroidogenesis

Acta Endocrinologica ◽

10.1530/eje.0.1300195 ◽

1994 ◽

Vol 130 (2) ◽

pp. 195-200 ◽

Cited By ~ 15

Author(s):

Barry D Albertson ◽

R Blake Hill ◽

Kellie A Sprague ◽

Karen E Wood ◽

Lynnette K Nieman ◽

...

Keyword(s):

Enzyme Activity ◽

Cynomolgus Macaque ◽

Sprague Dawley ◽

Steroidogenic Enzyme ◽

Monkey Model ◽

Ectopic Secretion ◽

Adrenal Steroidogenesis ◽

Sprague Dawley Rats

Albertson BD, Hill RB, Sprague KA, Wood KE, Nieman LK, Loriaux DL. Effect of the antiglucocorticoid RU486 on adrenal steroidogenic enzyme activity and steroidogenesis. Eur J Endocrinol 1994;130: 195–200. ISSN 0804–4643 RU486, a synthetic steroid receptor antagonist, has strong antiprogesterone and antiglucocorticoid properties. Chronic RU486 administration in two patients with ectopic secretion of adrenocorticotropin (ACTH) has been associated with decreasing plasma cortisol concentrations. One explanation of this finding is that RU486 may directly inhibit adrenal steroidogenesis. To test this hypothesis, we measured the effect of RU486 on specific steroidogenic enzymatic steps using an in vivo rat and an in vitro monkey model. Hypophysectomized–castrated–ACTH-replaced Sprague-Dawley rats were given RU486 i.p. at daily doses of 0, 0.0005, 0.005, 0.05, 0.5 and 5 mg/kg body weight per day for 7 days. The animals were sacrificed, and blood and adrenal glands collected. Adrenal cortical mitochondria and microsomes were purified from the rats and from two untreated Cynomolgus macaque monkeys. Specific steroidogenic enzyme activities were measured in the rat by the incorporation of 14C-labeled steroid substrates into products. A similar protocol was used to assay the steroidogenesis in the monkey adrenal fractions in the presence and absence of added RU486. Although rat adrenal weights decreased significantly at the highest RU486 dose, plasma levels of corticosterone were similar in control and treated rats. Rat adrenal 3β-hydroxysteroid dehydrogenase/isomerase (3-HSD), 21-hydroxylase (21-OH) and 11-hydroxylase (11-OH) activities decreased with increasing RU486 doses, with 21-OH and 11-OH being most severely affected. Monkey adrenal 3-HSD, 21-OH, 11-OH, 1 7-hydroxylase and 17,20-desmolase similarly decreased in the presence of increasing in vitro concentrations of RU486. Taken together, these results suggest that RU486 directly inhibits adrenal steroidogenesis, with a locus of action at several key enzymatic steps in the glucocorticoid pathway. This steroidogenic blockade may account for the observed decreases in glucocorticoids during RU486 treatment. Lynette K Nieman, Developmental Endocrinology Branch, NICHD, Building 10, Room 10N262, NIH, Bethesda, MD 20892, USA

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