Instructions for Participants in the Multicentre Evaluation Study of in Vitro Cytotoxicity (MEIC)

1988 ◽  
Vol 15 (3) ◽  
pp. 191-193
Author(s):  
Inger Bondesson ◽  
Björn Ekwall ◽  
Kjell Stenberg ◽  
Lennart Romert ◽  
Erik Walum
Keyword(s):  
Evaluation Study ◽  
In Vitro Cytotoxicity ◽  
Toxic Effects

A programme for a multicentre evaluation study of in vitro cytotoxicity (MEIC) has been proposed. The programme represents an attempt to evaluate the correlation between in vitro cytotoxicity and both lethal and sublethal toxic effects in man. Instructions for laboratories wishing to participate are given.

A Multicentre Evaluation Study of In Vitro Cytotoxicity

1987 ◽  
Vol 14 (3) ◽  
pp. 144-146
Author(s):  
V. Bernson ◽  
I. Bondesson ◽  
B. Ekwall ◽  
K. Stenberg ◽  
E. Walum
Keyword(s):  
Evaluation Study ◽  
In Vitro Cytotoxicity ◽  
Toxic Effects ◽  
Laboratory Animals ◽  
Toxicity Data ◽  
Animal Toxicity

A programme for a multicentre evaluation study of in vitro cytoxicity (MEIC) is proposed. The programme will try to evaluate the correlation between both lethal and sublethal toxic effects in man and in vitro cytotoxicity. Animal toxicity data will be included, to provide an opportunity for evaluating the species gap between man and laboratory animals. A list of chemicals to be used in this study is presented.

In vitro cytotoxicity and in vivo zebrafish toxicity evaluation of Ru(ii)/2-mercaptopyrimidine complexes

2019 ◽  
Vol 48 (18) ◽  
pp. 6026-6039 ◽  
Author(s):  
Vivianne S. Velozo-Sá ◽  
Luciano R. Pereira ◽  
Aliny P. Lima ◽  
Francyelli Mello-Andrade ◽  
Manuela R. M. Rezende ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Larval Development ◽  
In Vitro Cytotoxicity ◽  
Toxic Effects ◽  
Toxicity Evaluation ◽  
Embryonic And Larval Development

Ru(ii)/2-mercaptopyrimidine complexes active against cancer cells did not present toxic effects during embryonic and larval development of zebrafish.

scholarly journals Trypan Blue Exclusion Assay Verifies in Vitro Cytotoxicity of New Cis-Platinum (II) Complex in Human Cells

2019 ◽  
Vol 16 (3) ◽  
pp. 0555
Author(s):  
Hussein Et al.
Keyword(s):  
Cell Viability ◽  
Trypan Blue ◽  
Membrane Integrity ◽  
Platinum Complex ◽  
In Vitro Cytotoxicity ◽  
Toxic Effects ◽  
Polymorphonuclear Cells ◽  
Trypan Blue Exclusion ◽  
High Concentrations

          Various assays are used to determine the toxic effects of drugs at cellular levels in vitro.  One of these methods is the dye exclusion assay, which measures membrane integrity in the presence of Trypan blue. Trypan blue the dye which was used in this study to investigate cytotoxic effect of a new Cis –dichloroplatinum (II) complex [(Qu)2PtCl2] on the viability of polymorphonuclear cells (PMNs). Three concentrations of platinum complex were prepared (70, 35and 17.5 µg/ ml) and the results revealed that the percentage of cell viability decreased as the platinum complex concentration increased in comparison with control. The platinum complex exhibited low cytotoxic effects towards healthy cells at the concentrations of 17.5 µg/ ml and 35 µg/ ml, in which the percentage of cell viability was (77.01 ± 6.3) and (72.3± 0.50)respectively, with no significant differences as compared with the control(90.66 ±0.577). The viability was significantly decreased (67.59 ± 3.16) when the cells were treated with the concentration of 70 µg/ ml in comparison with control. These results indicated that the percentage of living cells decreased when treated with high concentrations of [(Qu)2PtCl2], which causes cells death, while low concentrations of the compound show low toxicity. This data indicates that this compound, at these concentrations may be suitable for use as a cancer treatment because it has low toxic effects on the healthy cells.        

scholarly journals Cytotoxic and toxicological effects of phthalimide derivatives on tumor and normal murine cells

2015 ◽  
Vol 87 (1) ◽  
pp. 313-330 ◽  
Author(s):  
PAULO MICHEL PINHEIRO FERREIRA ◽  
PATRICIA MARÇAL DA COSTA ◽  
ARINICE DE MENEZES COSTA ◽  
DAISY JEREISSATI BARBOSA LIMA ◽  
RENATA ROSADO DRUMOND ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Relative Weight ◽  
In Vitro Cytotoxicity ◽  
Toxic Effects ◽  
White Pulp ◽  
Sarcoma 180 ◽  
Peripheral Blood Mononuclear ◽  
Toxicological Effects

Eleven phthalimide derivatives were evaluated with regards to their antiproliferative activity on tumor and normal cells and possible toxic effects. Cytotoxic analyses were performed against murine tumors (Sarcoma 180 and B-16/F-10 cells) and peripheral blood mononuclear cells (PBMC) using MTT and Alamar Blue assays. Following, the investigation of cytotoxicity was executed by flow cytometry analysis and antitumoral and toxicological potential by in vivo techniques. The molecules 3b, 3c, 4 and 5 revealed in vitro cytotoxicity against Sarcoma 180, B-16/F-10 and PBMC. Since compound 4 was the most effective derivative, it was chosen to detail the mechanism of action after 24, 48 and 72 h exposure (22.5 and 45 µM). Sarcoma 180 cells treated with compound 4 showed membrane disruption, DNA fragmentation and mitochondrial depolarization in a time- and dose-dependent way. Compounds 3c, 4 and 5 (50 mg/kg/day) did not inhibit in vivotumor growth. Compound 4-treated animals exhibited an increase in total leukocytes, lymphocytes and spleen relative weight, a decreasing in neutrophils and hyperplasia of spleen white pulp. Treated animals presented reversible histological changes. Molecule 4 had in vitro antiproliferative action possibly triggered by apoptosis, reversible toxic effects on kidneys, spleen and livers and exhibited immunostimulant properties that can be explored to attack neoplasic cells.

Yeast Cells as a Test System for Evaluating the Toxicity of Chemicals

1990 ◽  
Vol 17 (3) ◽  
pp. 203-206
Author(s):  
Klaus Stadtlander ◽  
Heidemarie Lawohnus
Keyword(s):  
Partition Coefficients ◽  
Evaluation Study ◽  
Test System ◽  
In Vitro Cytotoxicity ◽  
Yeast Cells ◽  
Logarithmic Growth Phase ◽  
Very High ◽  
Logarithmic Growth ◽  
Key Parameter

The first 13 substances of the MEIC (multicentre evaluation study of in vitro cytotoxicity) project were tested in a test system in which the generation time of yeast cells in their logarithmic growth phase was used as the endpoint. Toxic effects, expressed as EC10, EC20 or EC50 values, were correlated with octanol/water partition coefficients. The correlation was found to be very high, indicating that the lipophilicity of substances is a key parameter for describing the toxicity of chemicals.

In vitro Cytotoxicity and Genotoxicity Analysis of Ten Tannery Chemicals Using SOS/umu Tests and High-content In vitro Micronucleus Tests

2018 ◽  
Vol 21 (4) ◽  
pp. 262-270 ◽  
Author(s):  
Zehao Huang ◽  
Na Li ◽  
Kaifeng Rao ◽  
Cuiting Liu ◽  
Zijian Wang ◽  
...  
Keyword(s):  
Micronucleus Test ◽  
A549 Cells ◽  
Quantitative Structure Activity Relationship ◽  
In Vitro Cytotoxicity ◽  
Cytotoxic Effects ◽  
Qsar Analysis ◽  
Cell Assays ◽  
Micronucleus Tests ◽  
Damaging Effects

Background: More than 2,000 chemicals have been used in the tannery industry. Although some tannery chemicals have been reported to have harmful effects on both human health and the environment, only a few have been subjected to genotoxicity and cytotoxicity evaluations. Objective: This study focused on cytotoxicity and genotoxicity of ten tannery chemicals widely used in China. Materials and Methods: DNA-damaging effects were measured using the SOS/umu test with Salmonella typhimurium TA1535/pSK1002. Chromosome-damaging and cytotoxic effects were determined with the high-content in vitro Micronucleus test (MN test) using the human-derived cell lines MGC-803 and A549. Conclusion: The cytotoxicity of the ten tannery chemicals differed somewhat between the two cell assays, with A549 cells being more sensitive than MGC-803 cells. None of the chemicals induced DNA damage before metabolism, but one was found to have DNA-damaging effects on metabolism. Four of the chemicals, DY64, SB1, DB71 and RR120, were found to have chromosome-damaging effects. A Quantitative Structure-Activity Relationship (QSAR) analysis indicated that one structural feature favouring chemical genotoxicity, Hacceptor-path3-Hacceptor, may contribute to the chromosome-damaging effects of the four MN-test-positive chemicals.

Mixed Micelles as Nano Polymer Therapeutics of Docetaxel: Increased In vitro Cytotoxicity and Decreased In vivo Toxicity

2018 ◽  
Vol 15 (4) ◽  
pp. 564-575 ◽  
Author(s):  
Arehalli S. Manjappa ◽  
Popat S. Kumbhar ◽  
Prajakta S. Khopade ◽  
Ajit B. Patil ◽  
John I. Disouza
Keyword(s):  
Mixed Micelles ◽  
In Vitro Cytotoxicity ◽  
Polymer Therapeutics ◽  
In Vivo Toxicity
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