Meningoencephalitis, Vasculitis, and Abortions Caused by Chlamydia pecorum in a Herd of Cattle

2021 ◽  
Vol 58 (3) ◽  
pp. 549-557
Author(s):  
Jason D. Struthers ◽  
Ailam Lim ◽  
Sylvia Ferguson ◽  
Jung Keun Lee ◽  
Clemence Chako ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Endothelial Cells ◽  
Differential Diagnosis ◽  
Mesothelial Cells ◽  
Sheep Flock ◽  
Third Trimester ◽  
Chain Reaction ◽  
Chlamydia Pecorum ◽  
Close Proximity ◽  
Polymerase Chain

A cow dairy ( n = 2000) in close proximity to a sheep flock had third-trimester abortions and fatalities in cows and calves over a 14-month period. Eighteen of 33 aborted fetuses (55%) had multifocal random suppurative or mononuclear meningoencephalitis with vasculitis. Seventeen of these affected fetuses had intracytoplasmic bacteria in endothelial cells, and 1 fetus with pericarditis had similar bacteria within mesothelial cells or macrophages. Immunohistochemistry for Chlamydia spp. or polymerase chain reaction (PCR) for Chlamydia pecorum or both, performed on brain or pooled tissue, were positive in all 14 tested fetuses that had meningoencephalitis and in 4/4 calves and in 3/4 tested cows that had meningoencephalitis and thrombotic vasculitis. In 1 calf and 11/11 fetuses, C. pecorum PCR amplicon sequences were 100% homologous to published C. pecorum sequences. Enzootic chlamydiosis due to C. pecorum was the identified cause of the late term abortions and the vasculitis and meningoencephalitis in fetuses, calves, and cows. C. pecorum, an uncommon bovine abortogenic agent, is a differential diagnosis in late-term aborted fetuses with meningoencephalitis, vasculitis, and polyserositis.

Polymerase chain reaction in the diagnosis of parotid gland tuberculosis

1998 ◽  
Vol 112 (5) ◽  
pp. 494-496 ◽  
Author(s):  
Enis Alpin Güneri ◽  
Ahmet Ömer İkiz ◽  
Nese Atabey ◽  
Özlem İzci ◽  
Semih Sütay
Keyword(s):  
Polymerase Chain Reaction ◽  
Differential Diagnosis ◽  
Parotid Gland ◽  
Mycobacterial Infection ◽  
Social Characteristics ◽  
Causative Organism ◽  
Chain Reaction ◽  
First Case ◽  
Polymerase Chain ◽  
High Degree

AbstractA parotid gland mass with presenting features of malignancy is a diagnostic and therapeutic challenge. The histological nature of the lesion must be clearly determined before proceeding with facial nerve sacrificing surgery. Although rare, tuberculosis of the parotid gland must be included in the differential diagnosis of a parotid gland mass especially when the social characteristics of the patient suggests a mycobacterial infection. Primary tuberculosis of the parotid gland is generally encountered among populations with a high incidence of pulmonary disease. The difficulty in the differential diagnosis of a parotid gland malignancy may be helped by a high degree of clinical suspicion, since laboratory tests generally do not identify the specific causative organism. This article reports the first case of parotid gland tuberculosis with clinical and radiodiagnostical features simulating malignancy in which the diagnosis was confirmed by the polymerase chain reaction (PCR).

scholarly journals Nonsuppurative Meningoencephalitis Associated with Aleutian Mink Disease Parvovirus Infection in Ranch Mink

2000 ◽  
Vol 12 (2) ◽  
pp. 159-162 ◽  
Author(s):  
N. W. Dyer ◽  
B. Ching ◽  
M. E. Bloom
Keyword(s):  
Polymerase Chain Reaction ◽  
Nervous System ◽  
Differential Diagnosis ◽  
Viral Genome ◽  
Brain Lesions ◽  
Chain Reaction ◽  
Mesenteric Lymph ◽  
Neurologic Disorders ◽  
Polymerase Chain ◽  
Ranch Mink

Severe nonsuppurative meningoencephalitis associated with Aleutian mink disease parvovirus (ADV) infection was observed in adult ranch mink. Brain lesions included severe, locally extensive to coalescing lymphoplasmacytic meningoencephalitis with accompanying gliosis, satellitosis, and mild extension of inflammation into the leptomeninges. ADV was identified in mesenteric lymph node, spleen, brain, and liver of affected mink by polymerase chain reaction techniques. Sequences of the ADV isolate (TH5) revealed 2 unique residues in the region of the viral genome that determines pathogenicity. These findings suggest that certain strains of ADV may preferentially cause disease in the nervous system. ADV infection should be considered in the differential diagnosis of neurologic disorders in mink.

Expression of Endothelin Peptides and Mrna in the Human Heart

1996 ◽  
Vol 90 (1) ◽  
pp. 37-46 ◽  
Author(s):  
C. Plumpton ◽  
M. J. Ashby ◽  
R. E. Kuc ◽  
G. O'Reilly ◽  
A. P. Davenport
Keyword(s):  
Polymerase Chain Reaction ◽  
Endothelial Cells ◽  
Reverse Transcriptase ◽  
Human Heart ◽  
Immunocytochemical Staining ◽  
Chain Reaction ◽  
Endothelin 1 ◽  
Terminal Fragment ◽  
Polymerase Chain ◽  
Endothelin 3

1. We have examined the expression of endothelin isoforms and their precursors in the human heart using RIA, HPLC, immunocytochemistry and reverse transcriptase—polymerase chain reaction assays. 2. Highly specific RIAs were used to measure the levels of mature endothelin and big endothelin-1 immunoreactivity in extracts of human right ventricle. There was no significant difference between samples from patients with ischaemic heart disease and idiopathic dilated cardiomyopathy. 3. HPLC coupled with RIAs allowed the separation and identification of the three mature isoforms of endothelin, big endothelin-1 and the C-terminal fragment of big endothelin-1. In extracts of human endocardial endothelial cells, peaks of immunoreactivity that co-eluted with authentic endothelin-1, big endothelin-1 and C-terminal fragment were found. 4. Intense immunocytochemical staining of mature endothelin immunoreactivity was detected in the cytoplasm of endothelial cells of all regions of the heart tested. Big endothelin-1 immunoreactivity mirrored that of the mature peptide and, in two of three individuals tested, big endothelin-2 immunoreactivity was also detected. No big endothelin-3 immunoreactivity was detected in any of the tissues examined. 5. Reverse transcriptase—polymerase chain reaction assays demonstrated endothelin-1 and endothelin-2 mRNA in all three samples of human left ventricle tested. In two of the individuals, additional bands were also detected with the endothelin-2 primers which corresponded to splice variants. There was no evidence for the expression of endothelin-3 mRNA. 6. These data suggest that endothelin-1 is the predominant isoform of endothelin in the human heart and is probably largely synthesized by the endothelial cells within the heart. If released from the endothelial cells in vivo, this potent cardiotonic peptide may play an important paracrine role in human cardiovascular function.

scholarly journals Resveratrol reduced the detrimental effects of malondialdehyde on human endothelial cells

2021 ◽  
Vol 13 (2) ◽  
pp. 131-140
Author(s):  
Mehdi Hassanpour ◽  
Çıgır Biray Avci ◽  
Reza Rahbarghazi ◽  
Aysa Rezabakhsh ◽  
Alireza Nourazarian ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Endothelial Cells ◽  
Surface Plasmon Resonance ◽  
Chromatin Remodeling ◽  
Tyrosine Kinases ◽  
Receptor Tyrosine Kinases ◽  
Pcr Array ◽  
Human Endothelial Cells ◽  
Chain Reaction ◽  
Polymerase Chain

Introduction: According to the statistics, vascular injury occurs during the onset of diabetic changes after the production of several byproducts. Many authorities have focused to find an alternative therapy for diabetic patients. In this study, we investigated the therapeutic effects of natural polyphenol like resveratrol on human endothelial cells exposed to malondialdehyde for 48 hours. Methods: Human Umbilical Vein Endothelial Cells were randomly classified into four groups;control, malondialdehyde (2.5 mM), resveratrol (100 μM), and cells received the combined regime for 48 hours. Cell viability was determined by 3-(4, 5-dimethyl thiazol-2-yl) 2, 5-diphenyl-tetrazoliumbromide (MTT) assay. Griess reaction was performed to measure the content of Nitric oxide (NO).Apoptosis was studied by using real-time polymerase chain reaction (RT-PCR) and western blotting assays. Levels of receptor tyrosine kinases like VEGFR-1, -2, Tie-1, and -2 were analyzed by enzyme-linked immunosorbent assay(ELISA). The affinity of resveratrol and malondialdehyde to serum albumin was measured by Surface Plasmon Resonance Assay. Any changes in chromatin remodeling were detected by PCR array analysis. Results: Resveratrol reduced cytotoxicity and NO content inside cells induced by malondialdehyde(MDA) (P < 0.05). Endothelial cell apoptosis was decreased by the reduction of pro-apoptotic factor Bax and increase of Bcl-2 following the incubation with resveratrol (P < 0.05). MDA-induced receptor tyrosine kinases increase was inhibited by resveratrol and reached near-to-normal levels (P < 0.05).Surface Plasmon Resonance revealed a higher affinity of resveratrol to albumin compared to the malondialdehyde-albumin complex. Polymerase chain reaction (PCR) array revealed the potency of resveratrol in chromatin remodeling following the treatment with malondialdehyde (P < 0.05). Conclusion: Based on our findings, resveratrol has the potential to decrease diabetic vascular injury induced by lipid byproducts such as MDA.

scholarly journals Ocular Tuberculosis with Progressive Unilateral Corneal Melting

2015 ◽  
Vol 6 (3) ◽  
pp. 293-297 ◽  
Author(s):  
Betül N. Bayraktutar ◽  
Ömür Uçakhan-Gündüz
Keyword(s):  
Polymerase Chain Reaction ◽  
Differential Diagnosis ◽  
Case Report ◽  
Biopsy Sample ◽  
Chain Reaction ◽  
Biopsy Material ◽  
Ocular Tuberculosis ◽  
Polymerase Chain ◽  
Corneal Melting ◽  
Peripheral Cornea

Objectives: In this case report, we present a patient with ocular tuberculosis presenting with progressive unilateral corneal melting. Patient: A 47-year-old female was admitted with melting at the inferior half of the peripheral cornea and inferior subconjunctival nodules. Biopsy material of the nodules was negative for tuberculosis bacillus. However, polymerase chain reaction of the biopsy sample revealed the DNA of the bacillus, and the diagnosis was confirmed. Conclusion: In endemic areas, ocular tuberculosis should be kept in mind in the differential diagnosis of patients with chronic and atypical corneal involvement.

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