scholarly journals Ultrastructure of a Vaginal Myxoma of a Rat

1974 ◽  
Vol 11 (4) ◽  
pp. 289-296 ◽  
Author(s):  
A. J. P. Klein-Szanto ◽  
C. J. Conti ◽  
R. E. Cartagenova

Ultrastructural study of a vaginal myxoma of an adult Wistar rat showed that the stroma consisted mainly of finely granular material with some fibers. The tumor cells contained numerous mitochondria, Golgi complexes, and associated vesicles, and moderate to abundant endoplasmic reticulum. Intracytoplasmic filaments and virus-like particles of about 120 nm were seen.

Author(s):  
S.R. Allegra

The respective roles of the ribo somes, endoplasmic reticulum, Golgi apparatus and perhaps nucleus in the synthesis and maturation of melanosomes is still the subject of some controversy. While the early melanosomes (premelanosomes) have been frequently demonstrated to originate as Golgi vesicles, it is undeniable that these structures can be formed in cells in which Golgi system is not found. This report was prompted by the findings in an essentially amelanotic human cellular blue nevus (melanocytoma) of two distinct lines of melanocytes one of which was devoid of any trace of Golgi apparatus while the other had normal complement of this organelle.


Author(s):  
J. C. Garancis ◽  
J. F. Kuzma ◽  
S. D. Wilson ◽  
E. H. Ellison

It has been proposed that a gastrin-like hormone elaborated by non-beta islet tumors of the pancreas may be responsible for a fulminating ulcer diathesis. Subsequently, a potent gastric secretagogue was isolated from ulcerogenic tumors of the pancreas. This disease process is known now as “Zollinger-Ellison syndrome”.In our studies of two cases of Zollinger-Ellison syndrome, pancreatic lesions were identified as alpha islet cell tumors (Fig. 1). Tumor cells were fairly uniform. The sizes of the alpha granules were not significantly different, but their number and distribution varied greatly from one cell to another. Each granule consisted of a round, highly dense central core, separated from the limiting membrane by an opaque zone. The granular form of the endoplasmic reticulum was particularly prominent. Numerous mitochondria, round or elongated, were dispersed throughout the cytoplasm. Individual or clusters of lysosomes were observed in the majority of cells.


Author(s):  
John R. Palisano

Although confronting cistemae (CC) have been observed in a variety of tumor cells and normal fetal rat, mouse, and human epithelial tissues, little is known about their origin or role in mitotic cells. While several investigators have suggested that CC arise from nuclear envelope (NE) folding back on itself during prophase, others have suggested that CC arise when fragments of NE pair with endoplasmic reticulum. An electron microscopic investigation of 0.25 um thick serial sections was undertaken to examine the origin of CC in HeLa cells.


1976 ◽  
Vol 54 (13) ◽  
pp. 1419-1437 ◽  
Author(s):  
Martha J. Powell

As the fungus Coelomomyces punctatus develops in the coelomic cavity of the mosquito Anopheles quadrimaculatus, the conformation of the plasma membrane and extracellular coat of the fungus changes markedly. The vegetative stage was surrounded by a granular and fibrillar extracellular coat which reacted positively in the silver methenamine procedure for the localization of polysaccharides. Numerous simple, branched or contorted cytoplasmic protuberances covered the irregularly shaped hyphal bodies. The surface of the hyphal body adjacent to the fat body of the mosquito had occasional involutions of the plasma membrane sheathed by cisternae of endoplasmic reticulum. In contrast with these hyphal bodies, cytoplasmic protuberances were spaced at wide intervals along filamentous hyphae. Aborting thalli were contorted and deeply lobed. The plasma membrane was smooth, and cytoplasmic protuberances were absent on other hyphae and hyphal bodies, particularly at advanced stages of infection. Instead unattached vesicles, morphologically similar to the protuberances found on some thalli, were embedded in granular material clustered around the smooth plasma membrane of these thalli. Mosquito hemocytes appeared to engulf these vesicles and granular material. As the vegetative stage was transformed into the reproductive stage, a newly formed, compact extracellular layer surrounded the sporangial initial. Later, a darkly staining wall appeared around the resting sporangium. Cisternae of endoplasmic reticulum consistently subtended thin areas in this pitted wall.


1993 ◽  
Vol 71 (8) ◽  
pp. 1039-1047 ◽  
Author(s):  
P. L. Polowick ◽  
V. K. Sawhney

Microspores undergo considerable ultrastructural changes between the tetrad and early binucleate microspore stages of microsporogenesis in tomato (Lycopersicon esculentum). Pollen wall deposition began late in the tetrad stage, and by the early microspore stage a lamellar foot layer and tectum were deposited. Sculpturing of the tectum was evident by the early binucleate microspore stage. Dictyosomes and vesicles were abundant during the period of pollen wall formation. Plastids were associated with the endoplasmic reticulum (ER) to form plastid–ER complexes, from the late tetrad to the vacuolate microspore stage. At the vacuolate microspore stage, endoplasmic reticulum independent of plastids was also observed, and at the early binucleate microspore stage ER was not associated with plastids. Free ribosomes were evenly distributed throughout the cytoplasm until the vacuolate microspore stage when they were organized into polysomes. Mitochondria were spherical to ellipsoid, with an electron-dense matrix and swollen cristae, until the early binucleate microspore stage when they were highly elongate and became convoluted. Key words: Lycopersicon esculentum, microsporogenesis, pollen development, tetrads, tomato, ultrastructure.


1980 ◽  
Vol 58 (22) ◽  
pp. 2367-2379
Author(s):  
Monique Colson

Osmic fixation was used in an ultrastructural study of Allium cepa seeds, both in the quiescent state and at the beginning of imbibition, in order to complete earlier observations made after glutaraldehyde fixation. In embryonic radicles, various groups of weakly stained lamellar structures looked like endoplasmic reticulum profiles. In these quiescent tissues, osmic fixation revealed many vesicles which appeared to be atypical elements of endoplasmic reticulum.In the first few hours of imbibition, reactive structures were more numerous and more diverse. Cisternae and vesicles of endoplasmic reticulum became typical looking, such as classically described in active tissues of other species.When Allium cepa seeds were treated with liquid nitrogen (−196 °C), induced structural alterations depended on the initial state of hydration. Lamellar systems underwent morphological changes. Osmic fixation showed the presence of numerous opaque globules throughout the cytoplasm. These globules seemed to derive from cisternae that appeared to have become fragmented during freezing. [Journal translation]


1974 ◽  
Vol 22 (6) ◽  
pp. 385-394 ◽  
Author(s):  
SANDRA KAZAHN MASUR ◽  
ERIC HOLTZMAN ◽  
F. CARTER BANCROFT

Cultured rat pituitary cells known to synthesize growth hormone were fixed and exposed to antigrowth hormone antibody conjugated to horseradish peroxidase. The cells were then incubated to demonstrate peroxidase activity. Reaction product was present within the endoplasmic reticulum, including the nuclear envelope. Study of several types of control preparations strongly suggest that the antibody-binding material in the endoplasmic reticulum is growth hormone.


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