Systemic Mastocytosis in a Goat

1995 ◽  
Vol 32 (6) ◽  
pp. 719-721 ◽  
Author(s):  
K. N. M. Khan ◽  
J. E. Sagartz ◽  
G. Koenig ◽  
K. Tanaka
Keyword(s):  
Electron Microscopy ◽  
Bone Marrow ◽  
Transmission Electron Microscopy ◽  
Mast Cells ◽  
Systemic Mastocytosis ◽  
Periodic Acid ◽  
Hypochromic Anemia ◽  
Postmortem Examination ◽  
Periodic Acid Schiff ◽  
Transmission Electron

Systemic mastocytosis was diagnosed in a 4-year-old, female Nubian goat. Clinically, the animal was depressed and had severe macrocytic hypochromic anemia and leukopenia. Postmortem examination revealed neoplastic mast cells invading the heart, lung, liver, spleen, lymph nodes, and bone marrow. Eosinophils were frequently admixed with infiltrating mast cells in all organs. Using routine light microscopy, histochemistry, and transmission electron microscopy, metachromatic and periodic acid—Schiff–positive granules were identified within the cytoplasm of neoplastic mast cells. Erythrophagocytosis was observed in some neoplastic cells, although its contribution to the anemia was not clear. This report represents the first description of mast cell neoplasia in the goat.

Hepatotoxic effects of melamine exposure from the weaning period in rats: a flow cytometric, electron microscopic, and histopathologic study

2021 ◽  
Author(s):  
Zuleyha Erisgin ◽  
Hasan Serdar Mutlu ◽  
Yavuz Tekelioglu ◽  
Engin Deveci ◽  
Ugur Seker
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Periodic Acid ◽  
Flow Cytometric Analysis ◽  
Control Group ◽  
Albino Rats ◽  
Histopathological Analysis ◽  
Periodic Acid Schiff ◽  
Flow Cytometric ◽  
Transmission Electron

Abstract This study aims to investigate the effects of melamine exposure from the weaning period (21st postnatal days in rats) on liver tissue. Female Wistar albino rats (n = 18) were divided into three groups. About 0.1-ml saline was applied to the control group by gavage for 21 days from the postnatal 21st day. The second group was taken 50-mg/kg melamine (in 0.1-ml saline) and the third group was taken 75-mg/kg melamine (in 0.1-ml saline) p.o. On the postnatal 45th day, all rats were sacrificed under anesthesia. Then, liver tissues were cut into three parts and two of them placed in neutral formalin for histopathological and flow cytometric analysis, and one of them placed in 2.5% glutaraldehyde. Histopathological analysis was performed with hematoxylin & eosin, Masson trichrome, periodic acid Schiff stained sections, and also with transmission electron microscopy. Apoptosis (Annexin V positivity) was analyzed by flow cytometry. According to histopathological analysis, hepatocyte damage, sinusoidal dilatation, and inflammatory cell infiltration significantly increased in both melamine groups compared with the control group. Apoptosis significantly increased in the 50 and 75-mg melamine groups compared with the control group. In the results of transmission electron microscopy analysis, there was abnormal chromatin distribution in the hepatocyte nuclei, loss in the cristae of the mitochondria, and organelle loss in large areas in the cytoplasm in both melamine exposure groups. As result, melamine exposure from the weaning period causes liver damage with increasing doses.

Magnesium and sulfur in mast cell and basophil granules of lower vertebrates in relation with histamine

1992 ◽  
Vol 50 (2) ◽  
pp. 1596-1597
Author(s):  
Kenichi Takaya
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Mast Cell ◽  
Mast Cells ◽  
Tree Frog ◽  
X Ray ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Fresh Frozen ◽  
Ultrathin Sections

Mast cell and basophil granules of the vertebrate contain heparin or related sulfated proteoglycans. Histamine is also present in mammalian mast cells and basophils. However, no histamine is detected in mast cell granules of the amphibian or fish, while it is shown in those of reptiles and birds A quantitative x-ray microanalysis of mast cell granules of fresh frozen dried ultrathin sections of the tongue of Wistar rats and tree frogs disclosed high concentrations of sulfur in rat mast cell granules and those of sulfur and magnesium in the tree frog granules. Their concentrations in tree frog mast cell granules were closely correlated (r=0.94).Fresh frozen dried ultrathin sections and fresh air-dried prints of the tree frog tongue and spleen and young red-eared turtle (ca. 6 g) spleen and heart blood were examined by a quantitative energy-dispersive x-ray microanalysis (X-650, Kevex-7000) for the element constituents of the granules of mast cells and basophils. The specimens were observed by transmission electron microscopy (TEM) (80-200 kV) and followed by scanning transmission electron microscopy (STEM) under an analytical electron microscope (X-650) at an acceleration voltage of 40 kV and a specimen current of 0.2 nA. A spot analysis was performed in a STEM mode for 100 s at a specimen current of 2 nA on the mast cell and basophil granules and other areas of the cells. Histamine was examined by the o-phthalaldehyde method.

scholarly journals A modified periodic acid-thiocarbohydrazide-silver proteinate staining sequence for enhanced contrast and resolution of glycogen depositions by transmission electron microscopy.

1987 ◽  
Vol 35 (3) ◽  
pp. 393-399 ◽  
Author(s):  
H K Lo ◽  
T I Malinin ◽  
G I Malinin
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Acetic Acid ◽  
Periodic Acid ◽  
Transmission Electron ◽  
Proposed Modification ◽  
Subsequent Staining

Oxidation of araldite-embedded liver sections by 1% w/v aqueous H5IO6 for 15 min and a 5-min reaction of carbonyls with 1% w/v thiocarbohydrazide in 10% v/v acetic acid was employed for subsequent staining of glycogen with silver-proteinate (S-P). The network of branching intracellular glycogen aggregates was revealed by 15-min staining with S-P, whereas 24 hr incubation in S-P was necessary to enhance the contrast of glycogen inclusions. We conclude that the proposed modification of glycogen staining readily affords the means for its localization at a desired level of contrast and resolution.

scholarly journals Storage pool deficiency in cattle with the Chediak-Higashi syndrome results from an absence of dense granule precursors in their megakaryocytes

Blood ◽  
1988 ◽  
Vol 72 (5) ◽  
pp. 1726-1734 ◽  
Author(s):  
M Menard ◽  
KM Meyers
Keyword(s):  
Electron Microscopy ◽  
Bone Marrow ◽  
Transmission Electron Microscopy ◽  
Membrane System ◽  
Dense Granule ◽  
Virtual Absence ◽  
Dense Granules ◽  
Storage Pool ◽  
Transmission Electron ◽  
Chediak Higashi Syndrome

Abstract Platelets from cattle with the Chediak-Higashi syndrome (CHS) have a storage pool deficiency and virtual absence of platelet dense granules. Megakaryocytes (MKs) from five control (n = 135) and five CHS (n = 133) cattle were evaluated using standard transmission electron microscopy. Osmiophilic dense granules were not observed in control or CHS MKs. In MKs from normal cattle, clear vesicles of 200- to 650-nm diameter bounded by a sharp membrane were observed. They were easily differentiated from the demarcation membrane system, endoplasmic reticulum, and alpha granules. The clear vesicles were virtually absent in MKs from CHS cattle at all stages of maturation. MKs in bone marrow samples from two control (n = 91) and two CHS (n = 61) cattle that had been processed for the uranaffin reaction were also evaluated. The clear vesicles were replaced by uranaffin-positive granules in MKs from control cattle, but positive uranaffin granules were not observed in CHS MKs. These findings indicate that the platelet dense granule storage pool deficiency in CHS cattle results from an anatomic absence of dense granule precursors in maturing and mature CHS MKs.

Effects of O3 on submucosal gland of bonnet monkey trachea

1983 ◽  
Vol 41 ◽  
pp. 678-679
Author(s):  
S. Yamashiro ◽  
D. Wilson ◽  
J. St. George ◽  
D. Hyde ◽  
C. Plopper ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Periodic Acid ◽  
Light And Electron Microscopy ◽  
Lung Parenchyma ◽  
Uranyl Acetate ◽  
Upper Airways ◽  
Periodic Acid Schiff ◽  
Transmission Electron ◽  
Bonnet Monkey ◽  
Submucosal Glands

In the past, ozone inhalation studies have focused on the lower airways and lung parenchyma. The purpose of this study was to evaluate the effects of ozone on submucosal glands of upper airways. Six adult male bonnet monkeys were exposed to 0.64 ppm ozone continuously for 7 days, and three were exposed to chamber conditions without ozone. The animals were exsanguinated under barbiturate anesthesia. The trachea and lung were fixed by airway infusion of Karnovsky's fixative, which was adjusted to pH 7.4 and 440 milliOsmols. Sagittal sections of ventral trachea were embedded in glycol methacrylate and Araldite 502 for light and electron microscopy. One micrometer methacrylate sections were stained with Alcian blue-periodic acid Schiff (AB/PAS). Selected areas of Araldite-embedded tissue were sectioned for transmission electron microscopy, stained with uranyl acetate and lead citrate and examined with a Zeiss EM 10. Volume percentages of the lumen, granular and nongranular regions of fhe gland and the duct wall, respectively, were estimated by stereologic methods on AB/PAS stained sections.

scholarly journals Variable opacity of glycogen in routine electron micrographs.

1977 ◽  
Vol 25 (9) ◽  
pp. 1069-1073 ◽  
Author(s):  
L E Thornell ◽  
M Sjöström ◽  
U Karlsson ◽  
E Cedergren
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Electron Micrographs ◽  
Periodic Acid ◽  
Frog Muscle ◽  
Nerve Terminals ◽  
Lead Citrate ◽  
Serial Sections ◽  
Transmission Electron ◽  
Reticular Zone

Glycogen in nerve terminals from the reticular zone of frog muscle was identified by transmission electron microscopy of both periodic acid-thiosemicarbazide-silverproteinate treated and UAc-PbCi-stained serial sections. A variable appearance of glycogen in the uranylacetate-lead citrate-stained nerve terminals was seen and is related to the preparative procedure. The study indicates the necessity of cytochemical identification for the assessment of glycogen organization in cells.

scholarly journals Method for Combined Observation of Serial Sections of Stented Arteries Embedded in Resin by Light Microscopy and Transmission Electron Microscopy

2018 ◽  
Vol 47 (3) ◽  
pp. 401-407 ◽  
Author(s):  
Atsushi Isobe ◽  
Kouichi Iwatani ◽  
Junko Souba ◽  
Hisako Terao ◽  
Hitomi Hagiwara ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscopy ◽  
Periodic Acid ◽  
Cell Junctions ◽  
Mixed Solution ◽  
Serial Sections ◽  
Thin Sections ◽  
Transmission Electron ◽  
Ultrathin Sections

We have developed a new method for obtaining information on whole tissues by light microscopy (LM) and ultrastructural features by transmission electron microscopy (TEM). This method uses serial sections of a stented artery embedded in resin. Stents were implanted in porcine coronary arteries in this study. The heart was perfusion fixed in a 2% paraformaldehyde and 1.25% glutaraldehyde mixed solution. The stented artery was then removed, fixed in 1% osmium, embedded in Quetol 651 resin, and sectioned serially. For LM, the black color of osmium was removed from the section by immersion in periodic acid and hydrogen peroxide after deplasticization. These sections were stained with hematoxylin and eosin and Elastica–Masson trichrome stain. For TEM, thin sections were re-embedded in Quetol 812 resin by the resupinate method and cut into ultrathin sections. A clear, fine structure was obtained, and organelles, microvilli, and cell junctions in the endothelium were easily observed. The combined observation of adjacent specimens by LM and TEM enabled us to relate histopathological changes in the millimeter scale to those in the nanometer scale.

Morphological and Histochemical Characterization of the Dermal Plates of Pleco (Hypostomus plecostomus)

2020 ◽  
Vol 26 (3) ◽  
pp. 551-566 ◽  
Author(s):  
Soha A. Soliman ◽  
Basma Mohamed Kamal ◽  
Alaa S. Abuo-Elhmad ◽  
Hanan H. Abd-Elhafeez
Keyword(s):  
Electron Microscopy ◽  
Periodic Acid ◽  
Detailed Structure ◽  
Periodic Acid Schiff ◽  
Transmission Electron ◽  
Different Types ◽  
Pas Staining ◽  
Precursor Layer ◽  
Histochemical Characterization

AbstractStudying the dermal skeleton in fish is valuable for phylogenetic specification. The current study describes the detailed structure of the plecostomus dermal skeleton, including its morphogenesis and distribution in the skin. The denticles have a crown and a basal part and are embedded in bony depressions, to which they are attached by denticle ligaments. During denticle morphogenesis, denticle papillae formed from denticle precursor cells align in two cellular layers: an outer ameloblast precursor layer and an inner odontoblast precursor layer. The ameloblast precursors and odontoblast precursors differentiate and secrete enamel and dentine, respectively. We used different histochemical techniques, including Crossmon's trichrome staining, Weigert–Van Gieson staining, periodic acid–Schiff (PAS) staining, combined Alcian blue (AB; pH 2.5)/PAS staining, Weigert–Van Gieson staining, Mallory trichrome staining, and AB staining to distinguish the dentine and denticle ligaments. We used acridine orange to detect lysosome activity during denticle eruption. Transmission electron microscopy was used to detect the denticle ultrastructure, and scanning electron microscopy was used to detect the topographic distributions of different types of dermal tissues in different anatomical regions.

Elemental composition of mast cell granules of the rat and tree frog: X-ray Microanalysis on fresh frozen dried ultrathin sections

1990 ◽  
Vol 48 (3) ◽  
pp. 356-357
Author(s):  
Kenichi Takaya
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Mast Cell ◽  
Mast Cells ◽  
Pancreatic Acinar Cells ◽  
Tree Frog ◽  
X Ray ◽  
Transmission Electron ◽  
Fresh Frozen ◽  
Ultrathin Sections

Electron probe x-ray microanalysis using fresh air-dried spreads revealed electrolyte elements in the granules of platelets, mast cells, pancreatic acinar cells and melanocytes. Mast cell granules of the rat and tree frog are quantitatively compared by an energy dispersive spectrometry (EDS) using fresh frozen dried ultrathin sections.Adult Wistar rats (ca. 250 g) and tree frogs (Hyla arborea japonica) of both sexes were used. Fresh frozen dried smears of rat peritoneal mast cells were prepared on the collodion-membrane covered titanium grid. Fresh frozen dried ultrathin sections of the tongue of the rat and tree frog were made by the metal contact method employing the rapid freezing apparatus (RF-2) cooled with liquid nitrogen. Ultrathin cryosections (60 nmj were cut at 163 K, transferred to the frozen specimen treating apparatus (FD-2) for freeze-drying in high vacuum at 173 K for 74-96 h. The specimens were observed first by 200kV transmission electron microscopy (TEM) and then under the scanning analytical electron microscope (X-650) for scanning transmission electron microscopy (STEM) images at an acceleration voltage of 40 kV and a specimen current of 0.2 nA.

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