Effects of O3 on submucosal gland of bonnet monkey trachea

1983 ◽  
Vol 41 ◽  
pp. 678-679
Author(s):  
S. Yamashiro ◽  
D. Wilson ◽  
J. St. George ◽  
D. Hyde ◽  
C. Plopper ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Periodic Acid ◽  
Light And Electron Microscopy ◽  
Lung Parenchyma ◽  
Uranyl Acetate ◽  
Upper Airways ◽  
Periodic Acid Schiff ◽  
Transmission Electron ◽  
Bonnet Monkey ◽  
Submucosal Glands

In the past, ozone inhalation studies have focused on the lower airways and lung parenchyma. The purpose of this study was to evaluate the effects of ozone on submucosal glands of upper airways. Six adult male bonnet monkeys were exposed to 0.64 ppm ozone continuously for 7 days, and three were exposed to chamber conditions without ozone. The animals were exsanguinated under barbiturate anesthesia. The trachea and lung were fixed by airway infusion of Karnovsky's fixative, which was adjusted to pH 7.4 and 440 milliOsmols. Sagittal sections of ventral trachea were embedded in glycol methacrylate and Araldite 502 for light and electron microscopy. One micrometer methacrylate sections were stained with Alcian blue-periodic acid Schiff (AB/PAS). Selected areas of Araldite-embedded tissue were sectioned for transmission electron microscopy, stained with uranyl acetate and lead citrate and examined with a Zeiss EM 10. Volume percentages of the lumen, granular and nongranular regions of fhe gland and the duct wall, respectively, were estimated by stereologic methods on AB/PAS stained sections.

Systemic Mastocytosis in a Goat

1995 ◽  
Vol 32 (6) ◽  
pp. 719-721 ◽  
Author(s):  
K. N. M. Khan ◽  
J. E. Sagartz ◽  
G. Koenig ◽  
K. Tanaka
Keyword(s):  
Electron Microscopy ◽  
Bone Marrow ◽  
Transmission Electron Microscopy ◽  
Mast Cells ◽  
Systemic Mastocytosis ◽  
Periodic Acid ◽  
Hypochromic Anemia ◽  
Postmortem Examination ◽  
Periodic Acid Schiff ◽  
Transmission Electron

Systemic mastocytosis was diagnosed in a 4-year-old, female Nubian goat. Clinically, the animal was depressed and had severe macrocytic hypochromic anemia and leukopenia. Postmortem examination revealed neoplastic mast cells invading the heart, lung, liver, spleen, lymph nodes, and bone marrow. Eosinophils were frequently admixed with infiltrating mast cells in all organs. Using routine light microscopy, histochemistry, and transmission electron microscopy, metachromatic and periodic acid—Schiff–positive granules were identified within the cytoplasm of neoplastic mast cells. Erythrophagocytosis was observed in some neoplastic cells, although its contribution to the anemia was not clear. This report represents the first description of mast cell neoplasia in the goat.

scholarly journals A MORPHOLOGIC AND CYTOCHEMICAL STUDY ON THE GREAT ALVEOLAR CELL

1966 ◽  
Vol 14 (12) ◽  
pp. 884-897 ◽  
Author(s):  
SERGEI P. SOROKIN
Keyword(s):  
Electron Microscopy ◽  
Periodic Acid ◽  
Light And Electron Microscopy ◽  
Multivesicular Bodies ◽  
Alveolar Cells ◽  
Periodic Acid Schiff ◽  
Alveolar Cell ◽  
Cytochemical Study ◽  
Lysosomal System ◽  
Chloroform Methanol

Lungs from marsupials, bats and rodents were studied by light and electron microscopy. In all three groups, the great alveolar cells exhibit similar morphologic and cytochemical characteristics. Cytoplasmic vacuoles seen in these cells by light microscopy correspond to cytosomes that are demonstrable in them by electron microscopy. Such cytosomes are osmiophilic, periodic acid-Schiff-positive and stainable with Sudan black after acetone extraction. After fixation in a mixture of aldehydes, followed by extraction in chloroform-methanol and postfixation in osmium tetroxide, cytosomes lose their osmiophilia. The cytoplasm of the great alveolar cell is notable for a loosely ordered granular endoplasmic reticulum, an extensive Golgi apparatus and numerous multivesicular bodies. Many forms transitional in appearance between multivesicular bodies and cytosomes are present. In these, osmiophilic matter occupies the intervesicular space. It is proposed that these bodies are the precursors of cytosomes. The cytosomes are interpreted to be products of the "lysosomal" system in this cell. Ultimately they are secreted onto the alveolar surface.

Periodic acid-thiocarbohydrazide-silver methenamine(PATS) reaction: An improved silver methodology for light and electron microscopy

1984 ◽  
Vol 42 ◽  
pp. 264-265 ◽  
Author(s):  
B. Giammara ◽  
T. Romaine ◽  
W. Ambrose ◽  
J. Hanker
Keyword(s):  
Electron Microscopy ◽  
Osmium Tetroxide ◽  
Periodic Acid ◽  
Patent Application ◽  
Light And Electron Microscopy ◽  
Basement Membranes ◽  
Full Description ◽  
Periodic Acid Schiff ◽  
Reticular Fibers ◽  
Pas Reaction

Many variations of the periodic acid-Schiff(PAS) reaction have been utilized for electron microscopy based on the Gomori periodic acid-silver methenamine reaction (1) or the periodic acid-thiocarbohydrazide-osmium tetroxide(PATCO) reaction (2,3). These reactions are widely employed and have been very useful for the demonstration of one or more biomacromolecules or structures such as glycogen, basement membranes, reticular fibers or lipopolysaccharide. However, these reactions have various drawbacks such as complexity of methodology, ability to stain only a limited number of these components, or lack of adaptability for both light and electron microscopy. Our newly devised PATS reaction is relatively easy to perform. A full description of the details must await the outcome of a pending patent application. It consists essentially of a stepwise treatment of the sample with periodic acid, thiocarbohydrazide(TCH) and silver methenamine.

Positive stain for light and electron microscopic demonstration of spirochetes in subgingival plaque and crevicular fluid samples from periodontal diseased sites

1989 ◽  
Vol 47 ◽  
pp. 1082-1083 ◽  
Author(s):  
B. Giammara ◽  
E.J. Burkes ◽  
R. Scruggs ◽  
G. Greco ◽  
P. Yates ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Periodic Acid ◽  
Light And Electron Microscopy ◽  
Subgingival Plaque ◽  
Microbial Count ◽  
Electron Microscopic ◽  
Microbial Composition ◽  
Periodic Acid Schiff ◽  
Clear Cut ◽  
Crevicular Fluid

In a recent study of 400 subgingival plaque samples from over 110 adult periodontitis patients, spirochetes were the overwhelming microbial type, averaging about 45% of the microbial count. This finding supports earlier arguments that spirochetes are pathognomonic in periodontal disease. Other studies had shown clear-cut differences in the microbial composition of healthy and diseased subgingival sites — the proportion of spirochetes being significantly higher in the latter. Another study indicated that periodontal deterioration at these sites could be predicted better by increased proportions of motile rods and spirochetes than by clinical measurements. However, spirochetes of all sizes and species do not show the same degree of association with periodontal breakdown. Moreover, spirochetes are usually difficult to culture and stain; they are generally monitored by darkfield or phase contrast microscopy.The PATS reaction, a modified periodic acid-Schiff(PAS) reaction which deposits silver for light and electron microscopy appears to stain Gram(-) bacteria positively as well as neutrophils and activated macrophages. When studying the stained Gram(-) bacteria on coverslip smears of subgingival plaque or crevicular fluid samples of patients by light microscopy, varying numbers of intensely stained spirochetes of different sizes were observed (Figs. 1,2). More spirochetes were usually seen in samples from diseased sites. After drying replicate PATS-stained coverslips with hexamethyldisilazane they were sputter coated with gold, and. then examined by the SEI and BEI modes of scanning electron microscopy (Figs. 3-6). A permanent record of the proportions of large, medium and small spirochetes at each site could thus be obtained. Generally, greater numbers of gram negative bacteria including some spirochetes were stained in samples from diseased sites. At some sites, however, spirochetes were the predominant microbes in both crevicular fluid and subgingival plaque (Fig. 1).

Hepatotoxic effects of melamine exposure from the weaning period in rats: a flow cytometric, electron microscopic, and histopathologic study

2021 ◽  
Author(s):  
Zuleyha Erisgin ◽  
Hasan Serdar Mutlu ◽  
Yavuz Tekelioglu ◽  
Engin Deveci ◽  
Ugur Seker
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Periodic Acid ◽  
Flow Cytometric Analysis ◽  
Control Group ◽  
Albino Rats ◽  
Histopathological Analysis ◽  
Periodic Acid Schiff ◽  
Flow Cytometric ◽  
Transmission Electron

Abstract This study aims to investigate the effects of melamine exposure from the weaning period (21st postnatal days in rats) on liver tissue. Female Wistar albino rats (n = 18) were divided into three groups. About 0.1-ml saline was applied to the control group by gavage for 21 days from the postnatal 21st day. The second group was taken 50-mg/kg melamine (in 0.1-ml saline) and the third group was taken 75-mg/kg melamine (in 0.1-ml saline) p.o. On the postnatal 45th day, all rats were sacrificed under anesthesia. Then, liver tissues were cut into three parts and two of them placed in neutral formalin for histopathological and flow cytometric analysis, and one of them placed in 2.5% glutaraldehyde. Histopathological analysis was performed with hematoxylin & eosin, Masson trichrome, periodic acid Schiff stained sections, and also with transmission electron microscopy. Apoptosis (Annexin V positivity) was analyzed by flow cytometry. According to histopathological analysis, hepatocyte damage, sinusoidal dilatation, and inflammatory cell infiltration significantly increased in both melamine groups compared with the control group. Apoptosis significantly increased in the 50 and 75-mg melamine groups compared with the control group. In the results of transmission electron microscopy analysis, there was abnormal chromatin distribution in the hepatocyte nuclei, loss in the cristae of the mitochondria, and organelle loss in large areas in the cytoplasm in both melamine exposure groups. As result, melamine exposure from the weaning period causes liver damage with increasing doses.

Banded Structure in a Hepatocellular Carcinoma

1977 ◽  
Vol 35 ◽  
pp. 510-511
Author(s):  
E. C. Chew ◽  
W. C. Chan
Keyword(s):  
Electron Microscopy ◽  
Hepatocellular Carcinoma ◽  
Osmium Tetroxide ◽  
Periodic Acid ◽  
Uranyl Acetate ◽  
Lead Citrate ◽  
Periodic Acid Schiff ◽  
Cacodylate Buffer ◽  
Tissue Area ◽  
Neural Tumor

Extracellular banded structures were first reported by Luse (1) in a neural tumor and subsequently by others in many types of tissues. This subject was summerized in detail by Sun and White in 1975 (2). This communication reports observations of banded structures discovered by electron microscopy in the study of a human hepatocellular carcinoma. The tissue was fixed in 3% glutaraldehyde in 0.1 M cacodylate buffer and post-fixed in buffered osmium tetroxide. Sections were stained with uranyl acetate and lead citrate. Thick sections, about 1 - 2 μ, were stained with periodic acid-Schiff's reagent involving heating of the slides.Banded structures are observed in the connective tissue area intermingled with collagen fibrils and are usually fusiform in shape (Fig. 1). The fusiform bodies average 0.5 μ in diameter and are outlined with periodicity of 800 to 1000 A°. Each period consists of a light and a dark band. Fine filaments of about 24 A° in thickness are present in the light bands (Fig. 2). They are also found to be periodic acid-Schiff positive (Fig. 3).

Morphological and Histochemical Characterization of the Dermal Plates of Pleco (Hypostomus plecostomus)

2020 ◽  
Vol 26 (3) ◽  
pp. 551-566 ◽  
Author(s):  
Soha A. Soliman ◽  
Basma Mohamed Kamal ◽  
Alaa S. Abuo-Elhmad ◽  
Hanan H. Abd-Elhafeez
Keyword(s):  
Electron Microscopy ◽  
Periodic Acid ◽  
Detailed Structure ◽  
Periodic Acid Schiff ◽  
Transmission Electron ◽  
Different Types ◽  
Pas Staining ◽  
Precursor Layer ◽  
Histochemical Characterization

AbstractStudying the dermal skeleton in fish is valuable for phylogenetic specification. The current study describes the detailed structure of the plecostomus dermal skeleton, including its morphogenesis and distribution in the skin. The denticles have a crown and a basal part and are embedded in bony depressions, to which they are attached by denticle ligaments. During denticle morphogenesis, denticle papillae formed from denticle precursor cells align in two cellular layers: an outer ameloblast precursor layer and an inner odontoblast precursor layer. The ameloblast precursors and odontoblast precursors differentiate and secrete enamel and dentine, respectively. We used different histochemical techniques, including Crossmon's trichrome staining, Weigert–Van Gieson staining, periodic acid–Schiff (PAS) staining, combined Alcian blue (AB; pH 2.5)/PAS staining, Weigert–Van Gieson staining, Mallory trichrome staining, and AB staining to distinguish the dentine and denticle ligaments. We used acridine orange to detect lysosome activity during denticle eruption. Transmission electron microscopy was used to detect the denticle ultrastructure, and scanning electron microscopy was used to detect the topographic distributions of different types of dermal tissues in different anatomical regions.

Ultrastructural analysis redefines light microscopic findings relative to morphological relationships among cells of the cerebral vasculature in the rat

1995 ◽  
Vol 53 ◽  
pp. 954-955
Author(s):  
Ruth V. W. Dimlich
Keyword(s):  
Phosphate Buffer ◽  
Periodic Acid ◽  
Ultrastructural Level ◽  
Uranyl Acetate ◽  
Sprague Dawley ◽  
Periodic Acid Schiff ◽  
Transmission Electron ◽  
Sprague Dawley Rats ◽  
Acetate Lead ◽  
Relationship Of

A previous light microscopic study determined that ~70% of all connective tissue mast cells (CTMCs) in the thalamus of the rat were adjacent to periodic acid-Schiff staining cells which also were ED2 positive therefore identified as a type of macrophage. Using transmission electron microscopy, the goal of the present study was to examine that association at the ultrastructural level and to determine the relationship of each of these cells to other components of the cerebrovasculature.Brains of anesthetized adult male Sprague-Dawley rats were fixed by intracardiac perfusion with paraformaldehyde and glutaraldehyde [2%:2.5% (phosphate buffer 0.1 M, pH 7.40 or 2%:2.5% for 5 min followed by 1%: 1.25% (phosphate buffer 80 mM, pH 7.4) for the remainder of the fixation]. Brains were sliced into ~1 mm sections and samples of thalamus were postfixed in OSO4 (1%, 0.1 M phosphate buffer), dehydrated, and flat embedded in LX112. Semi-thin (1 μm) and thin (60-90 nm) sections were cut and stained with toluidine blue and uranyl acetate/lead citrate respectively. Grids were viewed on a JOEL 100CXII electron microscope at 80kV.

scholarly journals SPERMIOGENESIS IN CANCER CRABS

1969 ◽  
Vol 43 (3) ◽  
pp. 575-603 ◽  
Author(s):  
Susan G. Langreth
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Nucleic Acid ◽  
Sperm Cell ◽  
Periodic Acid ◽  
Light And Electron Microscopy ◽  
Mature Sperm ◽  
Periodic Acid Schiff ◽  
The Core ◽  
Inner Region

Spermiogenesis in Cancer crabs was studied by light and electron microscopy. The sperm are aflagellate, and when mature consist primarily of a spherical acrosome surrounded by the nucleus with its short radiating arms. The acrosome forms by a coalescence of periodic acid-Schiff-positive (PAS-positive) vesicles. During spermiogenesis one edge of the acrosomal vesicle invaginates to form a PAS-negative central core. The inner region of the acrosome bounding the core contains basic proteins which are not complexed to nucleic acid. The formation of an elaborate lattice-like complex of fused membranes, principally from membranes of the endoplasmic reticulum, is described. These membranes are later taken into the nucleus and subsequently degenerate. In late spermatids, when most of the cytoplasm is sloughed, the nuclear envelope and the cell membrane apparently fuse to become the limiting boundary over most of the sperm cell. In the mature sperm the chromatin of the nucleus and arms, which is Feulgen-positive, contains no detectable protein. The chromatin filaments appear clumped, branched, and anastomosed; morphologically, they resemble the DNA of bacterial nuclei. Mitochondria are absent or degenerate in mature sperm of Cancer crabs, but the centrioles persist in the nucleoplasm at the base of the acrosome.

scholarly journals Correlative Light and Electron Microscopy (CLEM) Analysis of Nuclear Reorganization Induced by Clustered DNA Damage Upon Charged Particle Irradiation

2020 ◽  
Vol 21 (6) ◽  
pp. 1911 ◽  
Author(s):  
Susanne Tonnemacher ◽  
Mikhail Eltsov ◽  
Burkhard Jakob
Keyword(s):  
Electron Microscopy ◽  
Dna Damage ◽  
Light And Electron Microscopy ◽  
Ultrastructural Level ◽  
Uranyl Acetate ◽  
Dna Double Strand Breaks ◽  
Nuclear Reorganization ◽  
Particle Irradiation ◽  
Transmission Electron ◽  
Radiation Induced

Chromatin architecture plays major roles in gene regulation as well as in the repair of DNA damaged by endogenous or exogenous factors, such as after radiation. Opening up the chromatin might provide the necessary accessibility for the recruitment and binding of repair factors, thus facilitating timely and correct repair. The observed formation of ionizing radiation-induced foci (IRIF) of factors, such as 53BP1, upon induction of DNA double-strand breaks have been recently linked to local chromatin decompaction. Using correlative light and electron microscopy (CLEM) in combination with DNA-specific contrasting for transmission electron microscopy or tomography, we are able to show that at the ultrastructural level, these DNA damage domains reveal a chromatin compaction and organization not distinguishable from regular euchromatin upon irradiation with carbon or iron ions. Low Density Areas (LDAs) at sites of particle-induced DNA damage, as observed after unspecific uranyl acetate (UA)-staining, are thus unlikely to represent pure chromatin decompaction. RNA-specific terbium-citrate (Tb) staining suggests rather a reduced RNA density contributing to the LDA phenotype. Our observations are discussed in the view of liquid-like phase separation as one of the mechanisms of regulating DNA repair.

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