scholarly journals A Mouse Model of Delayed Rotator Cuff Repair Results in Persistent Muscle Atrophy and Fatty Infiltration

2018 ◽  
Vol 46 (12) ◽  
pp. 2981-2989 ◽  
Author(s):  
Zili Wang ◽  
Xuhui Liu ◽  
Michael R. Davies ◽  
Devante Horne ◽  
Hubert Kim ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Mouse Model ◽  
Mechanical Testing ◽  
Muscle Atrophy ◽  
Molecular Mechanisms ◽  
Ex Vivo ◽  
Fatty Infiltration ◽  
Shoulder Function ◽  
Muscle Weight ◽  
Orthopaedic Patients

Background: Rotator cuff (RC) tears are common tendon injuries seen in orthopaedic patients. Successful repair of large and massive RC tears remains a challenge due to our limited understanding of the pathophysiological features of this injury. Clinically relevant small animal models that can be used to study the pathophysiological response to repair are limited by the lack of chronic repair models. Purpose: To develop a highly clinically relevant mouse model of delayed RC repair. Study Design: Controlled laboratory study. Methods: Three-month-old C57BL/6J mice underwent unilateral supraspinatus (SS) and infraspinatus (IS) tendon tear with immediate, 2-week delayed, or 6-week delayed tendon repair. Animals with no repair or sham surgery served as controls. Gait analysis was conducted to measure shoulder function at 2 weeks and 6 weeks after surgery. Animals were sacrificed 6 weeks after the last surgery. Shoulder joint, SS, and IS muscles were harvested and analyzed histologically. Ex vivo mechanical testing of intact and repaired SS and IS tendons was conducted. Reverse-transcriptase polymerase chain reaction was performed on SS and IS muscles to quantify atrophy, fibrosis, and fatty infiltration–related gene expression. Results: Histological and tendon mechanical testing showed that torn tendons could be successfully repaired as late as 6 weeks after transection. However, significant atrophy and fatty infiltration of muscle, with impaired shoulder function, were persistent in the 6-week delayed repair group. Shoulder function correlated with the severity of RC muscle weight loss and fatty infiltration. Conclusion: We successfully developed a clinically relevant mouse model of delayed RC repair. Six-week delayed RC repair resulted in persistent muscle atrophy and fatty infiltration with inferior shoulder function compared with acute repair. Clinical Relevance: Our novel mouse model could serve as a powerful tool to understand the pathophysiological and cellular/molecular mechanisms of RC muscle and tendon degeneration, eventually improving our strategies for treating and repairing RC tears.

MRI quantification of fatty infiltration and muscle atrophy in a mouse model of rotator cuff tears

2012 ◽  
Vol 31 (3) ◽  
pp. 421-426 ◽  
Author(s):  
Sanjum P. Samagh ◽  
Erik J. Kramer ◽  
Gerd Melkus ◽  
Dominique Laron ◽  
Blake M. Bodendorfer ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Mouse Model ◽  
Muscle Atrophy ◽  
Fatty Infiltration ◽  
Rotator Cuff Tears

Rotator Cuff Fatty Infiltration and Muscle Atrophy: Relation to Glenoid Deformity in Primary Glenohumeral Osteoarthritis

2021 ◽  
Author(s):  
Michael A. Moverman ◽  
Richard N. Puzzitiello ◽  
Mariano E. Menendez ◽  
Nicholas R. Pagani ◽  
Paul-Anthony J. Hart ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Muscle Atrophy ◽  
Fatty Infiltration ◽  
Glenohumeral Osteoarthritis

scholarly journals Volumetric MicroCT Intensity Histograms of Fatty Infiltration Correlate with the Mechanical Strength of Rotator Cuff Repairs: An Ex Vivo Rabbit Model

2020 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Phillip E. McClellan ◽  
Lekha Kesavan ◽  
Yujing Wen ◽  
Jason Ina ◽  
Derrick M. Knapik ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Mechanical Strength ◽  
Ex Vivo ◽  
Fatty Infiltration ◽  
Rabbit Model

Does successful rotator cuff repair improve muscle atrophy and fatty infiltration of the rotator cuff?

2014 ◽  
Vol 23 (5) ◽  
pp. 604
Author(s):  
Noritaka Hamano ◽  
Atsushi Yamamoto ◽  
Tsutomu Kobayashi ◽  
Hitoshi Shitara ◽  
Tsuyoshi Ichinose ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Muscle Atrophy ◽  
Rotator Cuff Repair ◽  
Fatty Infiltration ◽  
Cuff Repair

scholarly journals Rotátorköpeny-szakadás után kialakult zsíros degeneráció változása az ín rekonstrukcióját követően, 65 éves kor után

2019 ◽  
Vol 160 (14) ◽  
pp. 533-539
Author(s):  
Imre Sallai ◽  
Márton Weidl ◽  
Attila Szatmári ◽  
Imre Antal ◽  
Gábor Skaliczki
Keyword(s):  
Rotator Cuff ◽  
Muscle Atrophy ◽  
Rotator Cuff Repair ◽  
Constant Score ◽  
Fatty Degeneration ◽  
Shoulder Function ◽  
Rotator Cuff Tears ◽  
Before And After ◽  
Cuff Repair

Abstract: Introduction: In the case of rotator cuff tears, the severity of the muscle atrophy and fatty degeneration has an effect on the success of the repair and on the functional outcome after surgery. Aim: The ability of regeneration reduces with ageing; therefore, the study examined the atrophy and the fatty degeneration after rotator cuff repair in patients over 65. Method: Eleven patients over 65 years of age were involved whose surgery was performed at the Department of Orthopaedics of Semmelweis University between 2012 and 2015. Their average age was 71.9 years and the average follow-up period was 39.9 months. Tear sizes were C1 in 3 cases, C2 in 3 cases, C3 in 4 cases, and C4 in 1 case. Each patient had magnetic resonance examination before and after the repair; the muscle atrophy and fatty degeneration were evaluated together with the type of the tear. Visual analogue scale and Constant score were used for the assessment of the pain and the shoulder function. Results: The average Constant score was 75 points. The occupancy ratio – referring to the severity of the atrophy – did not show significant improvement. The change in fatty degeneration and the atrophy were examined in different groups according to the size of the tears. In each group, the results showed progression. Conclusions: After rotator cuff repair in patients over 65, fatty degeneration and muscle atrophy also show progression. No significant relationship was found between the size of the tear and fatty degeneration or between the size of the tear and muscle atrophy. Orv Hetil. 2019; 160(14): 533–539.

scholarly journals Fatty Infiltration Is a Prognostic Marker of Muscle Function After Rotator Cuff Tear

2018 ◽  
Vol 46 (9) ◽  
pp. 2161-2169 ◽  
Author(s):  
Ana P. Valencia ◽  
Jim K. Lai ◽  
Shama R. Iyer ◽  
Katherine L. Mistretta ◽  
Espen E. Spangenburg ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Muscle Atrophy ◽  
Muscle Function ◽  
Contractile Function ◽  
Isometric Force ◽  
Fatty Infiltration ◽  
Collagen Content ◽  
Strongly Correlated ◽  
Computed Tomography Scanning ◽  
Maximal Isometric Force

Background: Massive rotator cuff tears (RCTs) begin as primary tendon injuries and cause a myriad of changes in the muscle, including atrophy, fatty infiltration (FI), and fibrosis. However, it is unclear which changes are most closely associated with muscle function. Purpose: To determine if FI of the supraspinatus muscle after acute RCT relates to short-term changes in muscle function. Study Design: Controlled laboratory study. Methods: Unilateral RCTs were induced in female rabbits via tenotomy of the supraspinatus and infraspinatus. Maximal isometric force and rate of fatigue were measured in the supraspinatus in vivo at 6 and 12 weeks after tenotomy. Computed tomography scanning was performed, followed by histologic analysis of myofiber size, FI, and fibrosis. Results: Tenotomy resulted in supraspinatus weakness, reduced myofiber size, FI, and fibrosis, but no differences were evident between 6 and 12 weeks after tenotomy except for increased collagen content at 12 weeks. FI was a predictor of supraspinatus weakness and was strongly correlated to force, even after accounting for muscle cross-sectional area. While muscle atrophy accounted for the loss in force in tenotomized muscles with minimal FI, it did not account for the greater loss in force in tenotomized muscles with the most FI. Collagen content was not strongly correlated with maximal isometric force, even when normalized to muscle size. Conclusion: After RCT, muscle atrophy results in the loss of contractile force from the supraspinatus, but exacerbated weakness is observed with increased FI. Therefore, the level of FI can help predict contractile function of torn rotator cuff muscles. Clinical Relevance: Markers to predict contractile function of RCTs will help determine the appropriate treatment to improve functional recovery after RCTs.

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