Biofabrication of controllable alginate hydrogel cell scaffolds based on bipolar electrochemistry

2021 ◽  
pp. 088391152110539
Author(s):  
Fei Xie ◽  
Changyue Li ◽  
Xiaoqing Hua ◽  
Li Ma
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Growth Model ◽  
Calcium Alginate ◽  
Three Dimensional ◽  
Alginate Hydrogel ◽  
Bipolar Electrodes ◽  
Cell Scaffolds ◽  
Alginate Hydrogels ◽  
Bipolar Electrochemistry

Bipolar electrochemistry successfully realized the electrodeposition of calcium alginate hydrogels in specific target areas in tissue engineering. However, the shape and quantity of three-dimensional cannot be accurately controlled. We presented a novel growth model for fabricating hydrogels based on bipolar electrochemical by patterned bipolar electrodes using photolithography. This work highlights pattern customization and quantitative control of hydrogels in cell culture platforms. Furthermore, alginate hydrogels with different heights can be controlled by adjusting the key parameters of the growth model. This strategy exhibits promising potential for cell-oriented scaffolds in tissue engineering.

scholarly journals Electrochemical Hydrogel Lithography of Calcium-Alginate Hydrogels for Cell Culture

Materials ◽  
2016 ◽  
Vol 9 (9) ◽  
pp. 744 ◽  
Author(s):  
Fumisato Ozawa ◽  
Kosuke Ino ◽  
Hitoshi Shiku ◽  
Tomokazu Matsue
Keyword(s):  
Cell Culture ◽  
Calcium Alginate ◽  
Alginate Hydrogels

scholarly journals Fabrication of 3D Printing Scaffold with Porcine Skin Decellularized Bio-Ink for Soft Tissue Engineering

Materials ◽  
2020 ◽  
Vol 13 (16) ◽  
pp. 3522
Author(s):  
Su Jeong Lee ◽  
Jun Hee Lee ◽  
Jisun Park ◽  
Wan Doo Kim ◽  
Su A Park
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
3D Printing ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Research Groups ◽  
Porcine Skin ◽  
Alginate Hydrogel ◽  
Chemical Treatments ◽  
Soft Tissue Engineering

Recently, many research groups have investigated three-dimensional (3D) bioprinting techniques for tissue engineering and regenerative medicine. The bio-ink used in 3D bioprinting is typically a combination of synthetic and natural materials. In this study, we prepared bio-ink containing porcine skin powder (PSP) to determine rheological properties, biocompatibility, and extracellular matrix (ECM) formation in cells in PSP-ink after 3D printing. PSP was extracted without cells by mechanical, enzymatic, and chemical treatments of porcine dermis tissue. Our developed PSP-containing bio-ink showed enhanced printability and biocompatibility. To identify whether the bio-ink was printable, the viscosity of bio-ink and alginate hydrogel was analyzed with different concentration of PSP. As the PSP concentration increased, viscosity also increased. To assess the biocompatibility of the PSP-containing bio-ink, cells mixed with bio-ink printed structures were measured using a live/dead assay and WST-1 assay. Nearly no dead cells were observed in the structure containing 10 mg/mL PSP-ink, indicating that the amounts of PSP-ink used were nontoxic. In conclusion, the proposed skin dermis decellularized bio-ink is a candidate for 3D bioprinting.

Direct Freeform Fabrication Technique for Bio-Manufacturing of Pre-Seeded, Spatially Heterogeneous, Anatomically-Shaped Alginate Hydrogel Implants

2005 ◽  
Author(s):  
Daniel L. Cohen ◽  
Evan Malone ◽  
Hod Lipson ◽  
Lawrence J. Bonassar
Keyword(s):  
Tissue Engineering ◽  
Solid Freeform Fabrication ◽  
Crosslinking Agent ◽  
Three Dimensional ◽  
Patient Specific ◽  
Efficient Production ◽  
Alginate Hydrogel ◽  
Freeform Fabrication ◽  
Patient Specific Implants ◽  
Multiple Material

A major challenge in orthopaedic tissue engineering is the generation of cell-seeded implants with structures that mimic native tissue, both in terms of anatomic geometries and intratissue cell distributions. By combining the strengths of injection molding tissue engineering with those of Solid Freeform Fabrication (SFF), three-dimensional pre-seeded implants were fabricated without custom-tooling, enabling efficient production of patient-specific implants. The incorporation of SFF technology also enables the fabrication of geometrically complex, multiple-material implants with spatially heterogeneous cell distributions that could not otherwise be produced. Using a custom-built robotic SFF platform and gel deposition tools, alginate hydrogel was used with calcium sulfate as a crosslinking agent to produce pre-seeded living implants of arbitrary geometries. The process was determined to be sterile and viable at 94±5%. The GAG production was found to be about half that of a similarly molded samples. The compressive elastic modulus was determined to be 1.462±0.113 kPa.

scholarly journals Biocomposite Inks for 3D Printing

2021 ◽  
Vol 8 (8) ◽  
pp. 102
Author(s):  
Gary Chinga-Carrasco
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
3D Printing ◽  
Drug Testing ◽  
Three Dimensional ◽  
Wound Dressings ◽  
Culture Models ◽  
Cell Culture Models

Three-dimensional (3D) printing has evolved massively during the last years and is demonstrating its potential in tissue engineering, wound dressings, cell culture models for drug testing, and prosthesis, to name a few [...]

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