The Effect of Hyaluronic Acid-supplemented Bone Graft in Bone Healing:                 Experimental Study in Rabbits

Background and Objectives: : Alloplastic is one of known materials have been used to treat bony defects in oral and maxillofacial region, but the healing is much more slower than auto graft . So many interpositional graft materials like hyaluronic acid and bone morphogenetic protein have been introduced to such material to induce osteoinductivity of graft material. This study aimed to evaluate the histological effect of Hyaluronic acid on bone graft substitute. Patients and methods: An experimental study carried out on 3 domestic sheep 2-4 years old. They divided into two groups, first group (study group): bone graft substitute mixed with hyaluronic acid introduced to the created cavities in the basal bone of mandible. Second group (control group): bone graft substitute were introduced into created cavities in the basal bone of mandible and the defects in both groups were covered with collagen membrane. The samples then sacrificed in deferent period interval 2 weeks, 6 weeks and 12 weeks and the sample collected and sent for histological examination. Results: all animals survived till the end of study. Both groups were characterized by new bone formation in defects however the bone formation in group 1 significantly preceded. Conclusion: hyaluronic acid induces the onset of of Oral and Maxillofacial department. Keywords: hyaluronic acid, Bone graft, Alloplastic material, guided bone regeneration.

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Collagen membrane versus platelet rich fibrin membrane in bone graft healing: An experimental study in sheep.

Erbil Dental Journal ◽

10.15218/edj.2020.15 ◽

2020 ◽

Vol 3 (2) ◽

pp. 104-111

Author(s):

Mahdi Abdullah ◽

Ahmed Hayder

Keyword(s):

Experimental Study ◽

Bone Graft ◽

Bone Healing ◽

Bone Substitute ◽

Low Cost ◽

Collagen Membrane ◽

Platelet Rich Fibrin ◽

Membrane Bone ◽

Significant Difference ◽

Fibrin Membrane

Background and objectives: Guided bone regeneration is a term used to define the use of barri-er membranes to promote complete osteogenesis. The aim of study was to histologically com-pare the effect of platelet rich fibrin membrane and resorbable Collagen membrane on bone substitute healing. Methods: in this experimental study three sheep’s which their weighted between 60-70 kg were used. After intramuscular sedation and local anesthesia, eighteen defects were created about 8 mm in diameter and 5 mm in depth in the lower border of mandible. Six defects on each animal which three in right side and three in left side of the mandible. The defects were filled with Biphasic calcium phosphate bone substitute. The first defect was covered by platelet rich fibrin membrane, the second one covered by Collagen membrane, and third one was left uncovered. The animals were sacrificed at 2, 6, and 12 weeks. Results: The healing of the grafted defects was histologically studied in all groups, and there was non-significant difference between PRF membrane and collagen membrane in healing of bone substitute at 2, 6, and 12 weeks.There was significant deference between PRF membrane and collagen membrane at 6 weekregarding scaffold replacement with mature bone. Conclusion: The use of PRF membrane shows a good outcome as a low cost biological material capable of improving bone healing defects, grafted with bone substitute. Keywords: Platelet rich fibrin membrane, collagen membrane, bone graft, bone healing.

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Effects of hyaluronic acid on bone graft healing: An experimental study on sheep

Erbil Dental Journal ◽

10.15218/edj.2019.15 ◽

2019 ◽

Vol 2 (2) ◽

pp. 236-242

Author(s):

Muhammed Mustafa ◽

Othman Omar

Keyword(s):

Experimental Study ◽

Hyaluronic Acid ◽

Bone Graft ◽

Graft Healing

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Influence of the proportion of particulate autogenous bone graft/platelet-rich plasma on bone healing in critical-size defects

Bone ◽

10.1016/j.bone.2009.04.246 ◽

2009 ◽

Vol 45 (2) ◽

pp. 339-345 ◽

Cited By ~ 39

Author(s):

Maria Nagata ◽

Michel Messora ◽

Roberta Okamoto ◽

Natália Campos ◽

Natália Pola ◽

...

Keyword(s):

Bone Graft ◽

Bone Healing ◽

Critical Size ◽

Platelet Rich Plasma ◽

Autogenous Bone Graft ◽

Autogenous Bone ◽

Critical Size Defects

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Experimental Study on Construction of Vascularized Bone Graft with Osteoinductive Calcium Phosphate Ceramics In Vivo

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.254-256.801 ◽

2003 ◽

Vol 254-256 ◽

pp. 801-804 ◽

Cited By ~ 7

Author(s):

C.Y. Bao ◽

Hong Song Fan ◽

Chun Lin Deng ◽

Y. Cao ◽

Y. Tan ◽

...

Keyword(s):

Experimental Study ◽

Calcium Phosphate ◽

Bone Graft ◽

Vascularized Bone Graft ◽

Calcium Phosphate Ceramics ◽

Vascularized Bone

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Mandible reconstruction and autogenous frozen bone graft: Experimental study on rats

Microsurgery ◽

10.1002/micr.1024 ◽

2001 ◽

Vol 21 (4) ◽

pp. 131-134 ◽

Cited By ~ 8

Author(s):

Cristina Garusi ◽

Luca Calabrese ◽

Gioacchino Giugliano ◽

Giovanni Mazzarol ◽

Stefano Podrecca ◽

...

Keyword(s):

Experimental Study ◽

Bone Graft ◽

Mandible Reconstruction ◽

Frozen Bone

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Results of an experimental study of hydrogel drainage containing ranibizumab in antiglaucoma operations

POINT OF VIEW EAST – WEST ◽

10.25276/2410-1257-2021-2-63-67 ◽

2021 ◽

pp. 63-67

Author(s):

I.I. Khusnitdinov ◽

Keyword(s):

Experimental Study ◽

Hyaluronic Acid ◽

Key Words ◽

Experimental Research ◽

Experimental Studies ◽

Glaucoma Surgery ◽

Control Group ◽

Morphological Studies

Purpose. Еxperimental substantiation of the effectiveness of biocompatible biodegradable hydrogels based on hyaluronic acid and chitosan succinate as a carrier of ranibizumab in antiglaucoma operations. Material and methods. Hydrogel drainage (HD) was obtained immediately before surgery. A solution of ranibizumab (0.23 ml) was mixed with a solution of hyaluronic acid dialdehyde (0.5 ml), then a solution of chitosan succinate (0.5 ml) was added. Experimental studies were performed in 12 (12 eyes) healthy rabbits. The first group consisted of 6 eyes – 0.187 ml of ranibizumab per 1 ml of gel. In the control group, HD was used intraoperatively without the addition of ranibizumab (6 eyes). Morphological studies were performed on 7th, 21st, and 42nd days. Results. In experimental studies in vitro and in vivo, it was proved that ranibizumab, administered as a part of 0.1 ml of hydrogel drainage in the antiglaucoma surgery area is released within 3 weeks and suppresses vascularization, scarring of the operating area, and preserves the intrascleral cavity. The optimal concentration of ranibizumab was selected-0.02 ml in 0.1 ml of gel. Conclusion. The safety and effectiveness of the use of hydrogel drainage with ranibizumab based on hyaluronic acid dialdehyde and chitosan succinate in anti-glaucoma operations has been proven. Key words: experimental research, hydrogel drainage, ranibizumab, glaucoma surgery.

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