scholarly journals Effects of hyaluronic acid on bone graft healing: An experimental study on sheep.

2020 ◽  
Vol 3 (2) ◽  
pp. 98-103
Author(s):  
Muhammed Mustafa ◽  
Othman Omar
Keyword(s):  
Experimental Study ◽  
Hyaluronic Acid ◽  
Bone Formation ◽  
Bone Graft ◽  
Bone Graft Substitute ◽  
Control Group ◽  
Collagen Membrane ◽  
Domestic Sheep ◽  
Graft Material ◽  
Basal Bone

Background and Objectives: : Alloplastic is one of known materials have been used to treat bony defects in oral and maxillofacial region, but the healing is much more slower than auto graft . So many interpositional graft materials like hyaluronic acid and bone morphogenetic protein have been introduced to such material to induce osteoinductivity of graft material. This study aimed to evaluate the histological effect of Hyaluronic acid on bone graft substitute. Patients and methods: An experimental study carried out on 3 domestic sheep 2-4 years old. They divided into two groups, first group (study group): bone graft substitute mixed with hyaluronic acid introduced to the created cavities in the basal bone of mandible. Second group (control group): bone graft substitute were introduced into created cavities in the basal bone of mandible and the defects in both groups were covered with collagen membrane. The samples then sacrificed in deferent period interval 2 weeks, 6 weeks and 12 weeks and the sample collected and sent for histological examination. Results: all animals survived till the end of study. Both groups were characterized by new bone formation in defects however the bone formation in group 1 significantly preceded. Conclusion: hyaluronic acid induces the onset of of Oral and Maxillofacial department. Keywords: hyaluronic acid, Bone graft, Alloplastic material, guided bone regeneration.

scholarly journals Biphasic Calcium Phosphate Sphere Graft Combined with a Double-Layer Non-Crosslinked Collagen Membrane Technique for Ridge Preservation: A Randomized Controlled Animal Study

Materials ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 18 ◽  
Author(s):  
Jungwon Lee ◽  
Young-Jun Lim ◽  
Bongju Kim ◽  
Ki-Tae Koo ◽  
Yong-Moo Lee
Keyword(s):  
Bone Graft ◽  
Double Layer ◽  
Single Layer ◽  
Test Group ◽  
Control Group ◽  
Collagen Membrane ◽  
Ridge Preservation ◽  
Graft Material ◽  
Membrane Technique ◽  
Layer Membrane

The purpose of this study was to compare the histologic and radiologic differences between single- and double-layer collagen membrane techniques in flapless ridge preservation. The mandibular fourth premolar and first molar of four beagle dogs were used in the experiment. Mesial roots of the teeth were extracted and root canal treatment was performed at the distal roots. Ridge preservation was performed at the extraction sites using synthetic bone graft material. A single layer (control group) or double layer (test group) of non-crosslinked collagen membrane was applied following bone graft application. Three months later, the animals were sacrificed and micro-computed tomography (micro-CT) and histomorphometric analyses were conducted. Nonparametric Mann–Whitney test was performed to compare between the control and test groups. The vertical difference between buccal and lingual crests of control and test groups was 1.28 ± 0.41 and 0.53 ± 0.37 mm, respectively (p = 0.026). The mineralized bone area in control and test groups was 31.48% ± 7.41% and 42.25% ± 9.73%, respectively (p = 0.041). Within the limit of this study, ridge preservation using the double-layer membrane technique showed a reduced buccal bone resorption and improved new-bone formation in the ridge compared to that using the single-layer membrane technique.

Porous Titanium Membranes Combined with Various Graft Materials Induce Exophytic Bone Formation in Rabbit Calvaria

2006 ◽  
Vol 309-311 ◽  
pp. 427-432 ◽  
Author(s):  
Y. Kim ◽  
Y.H. Kown ◽  
J.B. Park ◽  
J.H. Chung ◽  
H.N. Lim ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Graft ◽  
Porous Titanium ◽  
Control Group ◽  
Bovine Bone ◽  
Graft Material ◽  
New Bone Formation ◽  
Freeze Dried ◽  
Custom Made ◽  
Human Cancellous Bone

The purpose of this study was to examine if the application of custom-made porous titanium membranes combined with bone graft materials promotes exophytic bone formation in rabbit calvaria. For this purpose, round decorticated calvaria sites were created using a round carbide bur. In the control group, rectangular parallelepiped-shaped porous titanium membranes (RPTMs) were placed on the decorticated sites and fixed with metal pins. In the experimental groups, RPTMs were filled with one of the following bone graft materials prior to fixing with metal pins: bovine bone mineral (BBM), demineralized freeze-dried human cortical bone (DFDB) or freeze-dried human cancellous bone (FDB). Animals were sacrificed at 8 and 12 weeks after surgery, and new bone formation was assessed by histomorphometric as well as statistical analysis. The results indicate that at 8 and 12 weeks, all the experimental groups demonstrated exophytic bone formation. At 12 weeks, DFDB group revealed the most new bone formation (p<0.05) and resorption of grafted materials (p<0.05). On the basis of these findings, we conclude that RPTMs may be used as an augmentation membrane for guided bone regeneration and DFDB as an effective bone-inducing graft material.

Guided Tissue Regeneration of the Mixture of Human Tooth-Ash and Plaster of Paris in Dogs

2007 ◽  
Vol 330-332 ◽  
pp. 1327-1330 ◽  
Author(s):  
Ha Ra Ku ◽  
Hyun Seon Jang ◽  
S.G. Kim ◽  
Moon Jin Jeong ◽  
Joo Cheol Park ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Graft ◽  
Tissue Regeneration ◽  
Guided Tissue Regeneration ◽  
Human Tooth ◽  
Control Group ◽  
Graft Material ◽  
Bone Graft Material ◽  
Plaster Of Paris ◽  
Experimental Group

This study evaluated the efficacy of periodontal tissue regeneration using guided tissue regeneration (GTR) with a mixture of tooth ash and plaster as a bone substitute material in the treatment of class II furcation defects in dogs. GTR was performed at the site of a surgically formed mandibular premolar bifurcation area in dogs. Four adult dogs were used in the animal study. The animals were in a good periodontal state without any systemic disease. A resorbable membrane (Bio-Gide®, Swiss) and bone graft material using a mixture of tooth ash and plaster were used to examine the biological activity. The defect in the control group was filled a Bio-Gide® only. For the experimental group, the defects were filled with a mixture of tooth ash and plaster and covered by a Bio-Gide®. Oral rinsing with 0.12% chlorhexidine was performed twice a day for 2 weeks. In control group, new bone formation was observed adjacent to the preexisting bone. Soft tissue ingrowth was also noted. In experimental group, new lamellar type trabecular bone formation as well as centum and PDL regeneration was observed in the base of the infrabony pocket. More bone regeneration would be expected when GTR is performed using a mixture of human tooth-ash and plaster of Paris as a bone graft material.

Sinus Augmentation With Platelet-Rich Fibrin in Combination With Bovine Bone Graft Versus Bovine Bone Graft in Combination With Collagen Membrane

2015 ◽  
Vol 41 (5) ◽  
pp. 586-595 ◽  
Author(s):  
Nilufer Bolukbasi ◽  
Selim Ersanlı ◽  
Nurullah Keklikoglu ◽  
Cansu Basegmez ◽  
Tayfun Ozdemir
Keyword(s):  
Bone Graft ◽  
Test Group ◽  
Control Group ◽  
Collagen Membrane ◽  
Bovine Bone ◽  
Graft Material ◽  
Sinus Augmentation ◽  
Platelet Rich Fibrin ◽  
Bone Graft Material ◽  
Sinus Floor

The purpose of this study was to compare the efficacy between the use of bovine bone graft material and platelet-rich fibrin (PRF) mixture (test group) and bovine bone graft material and collagen membrane combination (control group) in 2-stage maxillary sinus augmentation. According to specific inclusion/exclusion criteria, patients treated between 2008 and 2012 were selected. Panoramic radiographs were used for radiologic assessments. To evaluate the relationship between sinus-graft height and each implant, the bone level (BL) was divided by implant length (IL). To evaluate the change in the height of grafted sinus, the grafted sinus floor above the lowest part of the original sinus height (GSH) was divided by the original sinus height (OSH). Samples taken during implant surgery were used for histologic and histomorphometric analyses. Twenty-five patients, 32 augmentation surgeries, and 66 one-stage implants were included in the study. No implant loss or complication was observed in either group. There were no statistical differences according to new bone formation (P = .61) and biomaterial remnant (P = .87). During the evaluation period, the test group showed statistically less change in the BL/IL ratio (P = .022). The difference of GSH/OSH ratio was found to be insignificant between groups (P = .093). It was observed that the grafted sinus covering the implant apex and sinus floor was above the original sinus height in both groups. It may be concluded from this study that both combinations can be successfully used for sinus augmentation. Further studies evaluating different graft materials and PRF combinations in the early phases of healing would be beneficial.

Effects of Autogenous Tooth Bone Graft and Platelet-Rich Fibrin in Peri-Implant Defects: An Experimental Study in an Animal Model

2020 ◽  
Vol 46 (3) ◽  
pp. 221-226
Author(s):  
Alper Kızıldağ ◽  
Ufuk Tasdemir ◽  
Taner Arabacı ◽  
Canan Aksu Kızıldağ ◽  
Mevlüt Albayrak ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Graft ◽  
Dental Implants ◽  
Bone Healing ◽  
Bone Defects ◽  
Control Group ◽  
Graft Material ◽  
New Bone Formation ◽  
Platelet Rich Fibrin ◽  
Bone To Implant Contact

The aim of this study was to evaluate the effect of autogenous tooth bone graft (ATBG) combined with platelet-rich fibrin (PRF) on bone healing in rabbit peri-implant osseous defects. Eighteen New Zealand rabbits were divided into 3 groups. Bone defects were prepared in each rabbit, and then an implant cavity was created in the defects. Dental implants were placed, and the peri-implant bone defects were treated with the following 3 methods: no graft material was applied in the control group, bone defects were treated with ATBG in the ATBG group, and bone defects were treated with ATBG combined with PRF in the ATBG+PRF group. After 28 days, the rabbits were sacrificed, and the dental implants with surrounding bone were removed. New bone formation and the percentage of bone-to-implant contact (BIC) were determined with histomorphometric evaluations. New bone formation was significantly higher in the ATBG+PRF group than the control and ATBG groups (P &lt; .05). In addition, BIC was significantly higher in the ATBG+PRF group than in the control and ATBG groups (P &lt; .05). The combination of ATBG with PRF contributed to bone healing in rabbits with peri-implant bone defects.

scholarly journals Preliminary Animal Study on Bone Formation Ability of Commercialized Particle-Type Bone Graft with Increased Operability by Hydrogel

Materials ◽  
2021 ◽  
Vol 14 (16) ◽  
pp. 4464
Author(s):  
So-Yeun Kim ◽  
You-Jin Lee ◽  
Won-Tak Cho ◽  
Su-Hyun Hwang ◽  
Soon-Chul Heo ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Graft ◽  
Cellular Response ◽  
Human Mesenchymal Stem Cells ◽  
Study Groups ◽  
Control Group ◽  
Graft Material ◽  
Defect Sites ◽  
Significant Difference

The purpose of this study was to evaluate the bone-generating ability of a new bovine-derived xenograft (S1-XB) containing hydrogel. For control purposes, we used Bio-Oss and Bone-XB bovine-derived xenografts. S1-XB was produced by mixing Bone-XB and hydrogel. Cell proliferation and differentiation studies were performed to assess cytotoxicities and cell responses. For in vivo study, 8 mm-sized cranial defects were formed in 16 rabbits, and then the bone substitutes were transplanted into defect sites in the four study groups, that is, a Bio-Oss group, a Bone-XB group, an S1-XB group, and a control (all n = 4); in the control group defects were left empty. Eight weeks after surgery, new bone formation areas were measured histomorphometrically. In the cell study, extracts of Bio-Oss, Bone-XB, and S1-XB showed good results in terms of the osteogenic differentiation of human mesenchymal stem cells (hMSCs) and no cytotoxic reaction was evident. No significant difference was observed between mean new bone areas in the Bio-Oss (36.93 ± 4.27%), Bone-XB (35.07 ± 3.23%), and S1-XB (30.80 ± 6.41%) groups, but new bone area was significantly smaller in the control group (18.73 ± 5.59%) (p < 0.05). Bovine-derived bone graft material containing hydrogel (S1-XB) had a better cellular response and an osteogenic effect similar to Bio-Oss.

Results of an experimental study of hydrogel drainage containing ranibizumab in antiglaucoma operations

2021 ◽  
pp. 63-67
Author(s):  
I.I. Khusnitdinov ◽  
Keyword(s):  
Experimental Study ◽  
Hyaluronic Acid ◽  
Key Words ◽  
Experimental Research ◽  
Experimental Studies ◽  
Glaucoma Surgery ◽  
Control Group ◽  
Morphological Studies

Purpose. Еxperimental substantiation of the effectiveness of biocompatible biodegradable hydrogels based on hyaluronic acid and chitosan succinate as a carrier of ranibizumab in antiglaucoma operations. Material and methods. Hydrogel drainage (HD) was obtained immediately before surgery. A solution of ranibizumab (0.23 ml) was mixed with a solution of hyaluronic acid dialdehyde (0.5 ml), then a solution of chitosan succinate (0.5 ml) was added. Experimental studies were performed in 12 (12 eyes) healthy rabbits. The first group consisted of 6 eyes – 0.187 ml of ranibizumab per 1 ml of gel. In the control group, HD was used intraoperatively without the addition of ranibizumab (6 eyes). Morphological studies were performed on 7th, 21st, and 42nd days. Results. In experimental studies in vitro and in vivo, it was proved that ranibizumab, administered as a part of 0.1 ml of hydrogel drainage in the antiglaucoma surgery area is released within 3 weeks and suppresses vascularization, scarring of the operating area, and preserves the intrascleral cavity. The optimal concentration of ranibizumab was selected-0.02 ml in 0.1 ml of gel. Conclusion. The safety and effectiveness of the use of hydrogel drainage with ranibizumab based on hyaluronic acid dialdehyde and chitosan succinate in anti-glaucoma operations has been proven. Key words: experimental research, hydrogel drainage, ranibizumab, glaucoma surgery.

scholarly journals Histomorphometric Evaluation of Socket Preservation Using Autogenous Tooth Biomaterial and BM-MSC in Dogs

Scanning ◽  
2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Jin-Hyun Kim ◽  
Puneet Wadhwa ◽  
HongXin Cai ◽  
Dong-Hyung Kim ◽  
Bing Cheng Zhao ◽  
...  
Keyword(s):  
Bone Graft ◽  
Alveolar Bone ◽  
Control Group ◽  
Ridge Preservation ◽  
Graft Material ◽  
Socket Preservation ◽  
Bone Graft Material ◽  
Premolar Extraction ◽  
Bone Width ◽  
Significant Difference

This study is aimed at assessing the dimensional alterations occurring in the alveolar bone after premolar extraction in dogs with histomorphometric and histological analysis. After atraumatic premolar extraction, tooth-derived bone graft material was grafted in the extraction socket of the premolar region in the lower jaws of six dogs in two experimental groups. In the second experimental group, BM-MSCs were added together with the graft. The control was left untreated on the opposite side. After twelve weeks, all six animals were sacrificed. Differences in alveolar bone height crests lingually and buccally, and alveolar bone width at 1, 3, and 5 mm infracrestally, were examined. Histologic study revealed osteoconductive properties of tooth biomaterial. A statistically significant difference was detected between the test and control groups. In the test groups, a reduced loss of vertical and horizontal alveolar bone dimensions compared with the control group was observed. Tooth bone graft material may be considered useful for alveolar ridge preservation after tooth extraction, as it could limit the natural bone resorption process.

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