Long-Term Treatment of HIV-Infected MT-4 Cells in Culture with HIV Proteinase Inhibitor RO 31-8959 Leads to Complete Cure of Infection

An HIV-infected cell culture was treated with the specific HIV proteinase inhibitor Ro 31-8959 for three months to analyse the antiviral effect and possible cytotoxicity of the drug in long-term treatment. The drug was added 1 h after HIV infection with 0.002 m.o.i. and maintained for 87 days in the cell culture. There was no detectable cell death nor any evidence of HIV production in this time. Cells were proven to be initially infected, since premature drug removal led to a re-emergence of infectious HIV and cell death. However, after 87 days of treatment the drug could be removed safely and HIV was cleared demonstrably from the culture. These data suggest that long-term dosage may be advantageous to the clinical treatment of HIV infection by HIV proteinase inhibitors.

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Long-term treatment for lipoatrophy associated or not with HIV infection using ePTFE implants and polyacrylamide gel

Aesthetic Surgery Journal ◽

10.1016/j.asj.2005.09.006 ◽

2005 ◽

Vol 25 (6) ◽

pp. 561-570 ◽

Cited By ~ 5

Author(s):

B MOLE

Keyword(s):

Hiv Infection ◽

Term Treatment ◽

Polyacrylamide Gel ◽

Long Term Treatment

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Long-Term Treatment of Anterior Pituitary Cells with Nitric Oxide Induces Programmed Cell Death

Endocrinology ◽

10.1210/en.2003-1263 ◽

2004 ◽

Vol 145 (4) ◽

pp. 2064-2070 ◽

Cited By ~ 13

Author(s):

Miguel Omar Velardez ◽

Ariel Hernán Poliandri ◽

Jimena Paula Cabilla ◽

Cristian Carlos Armando Bodo ◽

Leticia Inés Machiavelli ◽

...

Keyword(s):

Nitric Oxide ◽

Cell Death ◽

Programmed Cell Death ◽

Anterior Pituitary ◽

Term Treatment ◽

Pituitary Cells ◽

Anterior Pituitary Cells ◽

Long Term Treatment

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Renal function in Japanese HIV-1-positive patients who switch to tenofovir alafenamide fumarate after long-term tenofovir disoproxil fumarate

10.21203/rs.3.rs-265066/v1 ◽

2021 ◽

Author(s):

Kensuke Abe ◽

Taku Obara ◽

Satomi Kamio ◽

Asahi Kondo ◽

Junji Imamura ◽

...

Keyword(s):

Renal Function ◽

Renal Dysfunction ◽

Tenofovir Disoproxil Fumarate ◽

Antiviral Effect ◽

Term Treatment ◽

Long Term Treatment ◽

The Difference ◽

Tenofovir Alafenamide ◽

Hiv 1

Abstract BACKGROUND Tenofovir disoproxil fumarate (TDF) has a strong antiviral effect, but TDF is known to cause renal dysfunction. Therefore, we are investigating preventing renal dysfunction by replacing TDF with tenofovir alafenamide fumarate (TAF), which is known to be relatively safe to the kidneys. However, the changes in renal function under long-term use of TAF are not known. In this study, we evaluated renal function in Japanese HIV-1-positive patients switching to TAF after long-term treatment with TDF.METHODS A single-center observational study was conducted in Japanese HIV-1-positive patients. TDF was switched to TAF after at least 48 weeks of treatment so we could evaluate the long-term use of TDF. The primary endpoint was the estimated glomerular filtration rate (eGFR) at 144 weeks of TAF administration. In addition, we predicted the factors that would lead to changes in eGFR after long-term use of TAF.RESULTS Of the 125 HIV-1-positive patients who were prescribed TAF at our hospital during the study period, 70 fulfilled the study criteria. The eGFR at the time of switching from TDF to TAF was 81.4 ± 21.1 mL/min/1.73 m2. eGFR improved significantly after 12 weeks of taking TAF but significantly decreased at 96 and 144 weeks. At 144 weeks of taking TAF, the factors significantly correlated with the difference in eGFR from baseline were the difference in eGFR at 48 weeks of taking TAF and age at the start of TAF.CONCLUSIONS In this study, Japanese HIV-1-positive patients who had been taking TDF for a long period of time showed a decrease in eGFR after switching to long-term use of TAF. Japanese HIV-1-positive patients are expected to take TAF for a long time. Depending on age, laboratory values related to renal function need to be monitored carefully.

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