Mycoplasma genitalium infections in Cuba: surveillance of urogenital syndromes, 2014–2015

2018 ◽  
Vol 29 (10) ◽  
pp. 994-998 ◽  
Author(s):  
Brian A Mondeja ◽  
Nadia M Rodríguez ◽  
Orestes Blanco ◽  
Carmen Fernández ◽  
Jørgen S Jensen
Keyword(s):  
Polymerase Chain Reaction ◽  
Reproductive Tract ◽  
Mycoplasma Genitalium ◽  
Reference Laboratory ◽  
Sexually Active ◽  
Chain Reaction ◽  
Transmitted Infection ◽  
Men And Women ◽  
Sexually Transmitted ◽  
Polymerase Chain

Mycoplasma genitalium is an emerging sexually transmitted pathogen implicated in urethritis in men and several inflammatory reproductive tract syndromes in women. The prevalence of M. genitalium infections in Cuban patients with urogenital syndromes is unknown. The aim of this study was to analyse the prevalence of M. genitalium infection in sexually-active Cuban men and women with urogenital syndromes as a part of aetiological surveillance of urogenital syndromes in Cuba. Samples from men and women with urogenital syndromes submitted to the Mycoplasma Reference Laboratory for mycoplasma diagnosis from 1 January 2014 to 1 June 2015 were analysed by polymerase chain reaction (PCR) for detection of M. genitalium. A total of 971 samples were received and processed. Of the patients tested, 5.7% (47/824) of women and 27.9% (41/147) of men were positive for M. genitalium. This paper presents the largest study of M. genitalium infections among Cuban patients with urogenital syndromes and is Cuba’s first M. genitalium survey. We suggest that M. genitalium should be considered in the Cuban sexually transmitted infection management protocols as an important pathogen, particularly in men.

scholarly journals Detection of Neisseria gonorrhoeae in Palestinian women using polymerase chain reaction

2010 ◽  
Vol 4 (4) ◽  
pp. 637-640
Author(s):  
Mohammad A. Farraj ◽  
Gabi M. Abusada ◽  
Abed Alraoof M. Saleem ◽  
Ayyub Y. Joaidi ◽  
Raba M. Radad ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Transmitted Disease ◽  
Rectal Mucosa ◽  
Molecular Techniques ◽  
Chain Reaction ◽  
Specific Primers ◽  
Men And Women ◽  
Sexually Transmitted ◽  
Palestinian Women ◽  
Polymerase Chain

Abstract Background: Neisseria gonorrohoeae is an exclusive human pathogen that primarily infects the urogenital epithelia. Infections caused by N. gonorrhoeae are considered the second major cause of sexually transmitted disease after Chlamydiae worldwide. Although the urethra and the uterine cervix serve as the initial sites for gonococcal infections in men and women, infection of the conjunctiva, pharynx, tendons, joints, as well as rectal mucosa are also reported. Objectives: The objectives of this study were to introduce molecular techniques such as polymerase chain reaction (PCR) to detect N. gonorrhoeae directly from endocervical swabs. In addition, it provides a picture of Neisseria gonorrohea infection among a sample of Palestinian women in West Bank. Methods: Two hundred and thirteen endocervical swabs were collected from sexually active married women with endocervical abnormalities attending healthcare clinics. DNA was extracted directly from the swabs and PCR was performed using specific primers targeting the orf1 region of the genome. Results: The results obtained indicated that the percentage of positive cases of N. gonorrhoeae among the women tested was 1.40%. Conclusion: Implementing guidelines for comprehensive screening of men and women with more sensitive tests may improve detection and management of sexually transmitted infections.

scholarly journals Detection of Mycoplasma genitalium from Male Patients with Non-Gonococcal Urethritis by Polymerase Chain Reaction

1994 ◽  
Vol 68 (11) ◽  
pp. 1376-1380
Author(s):  
Hisao KOMEDA ◽  
Takashi DEGUCHI ◽  
Mitsuru YASUDA ◽  
Kouji TADA ◽  
Hideki IWATA ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycoplasma Genitalium ◽  
Chain Reaction ◽  
Gonococcal Urethritis ◽  
Male Patients ◽  
Polymerase Chain

scholarly journals Critical analysis: use of polymerase chain reaction to diagnose leprosy

2016 ◽  
Vol 52 (1) ◽  
pp. 163-169 ◽  
Author(s):  
Flaviane Granero Maltempe ◽  
Vanessa Pietrowski Baldin ◽  
Mariana Aparecida Lopes ◽  
Vera Lúcia Dias Siqueira ◽  
Regiane Bertin de Lima Scodro ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Globin Gene ◽  
Public Health Problem ◽  
Reference Laboratory ◽  
Chain Reaction ◽  
Molecular Biology Technique ◽  
Pcr Inhibitor ◽  
Significant Difference ◽  
Polymerase Chain ◽  
Pcr Techniques

ABSTRACT Leprosy is a neglected tropical disease and an important public health problem, especially in developing countries. It is a chronic infectious disease that is caused by Mycobacterium leprae, which has a predilection for the skin and peripheral nerves. Although it has low sensitivity, slit-skin smear (SSS) remains the conventional auxiliary laboratory technique for the clinical diagnosis of leprosy. Polymerase chain reaction (PCR) is a molecular biology technique that holds promise as a simple and sensitive diagnostic tool. In the present study, the performance of two PCR methods, using different targets, PCR-LP and PCR-P, were compared with SSS with regard to leprosy diagnosis in a reference laboratory. M. leprae DNA was extracted from 106 lymph samples of 40 patients who had clinical suspicion of leprosy. The samples were subjected to both PCR techniques and SSS. Amplification of the human b-globin gene was used as PCR inhibitor control. The specificity of both PCR techniques was 100%, and sensitivity was 0.007 and 0.015 µg/ml for PCR-LP and PCR-P, respectively. No significant difference was found between either the PCR-LP or PCR-P results and SSS results (p > 0.05). Although PCR is not yet a replacement for SSS in the diagnosis of leprosy, this technique may be used as an efficient auxiliary tool for early detection of the disease, especially in endemic regions. This strategy may also be useful in cases in which SSS results are negative (e.g., in paucibacillary patients) and cases in which skin biopsy cannot be performed.

scholarly journals Chlamydia trachomatis detection in HIV infected patients using polymerase chain reaction

2013 ◽  
Vol 2 (1) ◽  
pp. 12-16
Author(s):  
A Shrestha ◽  
N Adhikari ◽  
Y Shah ◽  
P Poudel ◽  
B Acharya ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Public Health Problem ◽  
Chlamydia Infection ◽  
Hiv Positive ◽  
Chain Reaction ◽  
Hiv Patients ◽  
Important Public Health Problem ◽  
Sexually Transmitted ◽  
Polymerase Chain

Introduction: Chlamydia trachomatis is a sexually transmitted organism and causes important public health problem in the sexually active age group. Limited studies are found regarding the prevalence of C. trachomatis in Nepal. Moreover, currently there are no any study in Nepal reporting the association of chlamydia and HIV infection. This study attempts to determine the burden of chlamydia on HIV positive patients. Materials and Methods: A total of 117 HIV positive patients visiting a HIV clinic in Kathmandu, were screened for chlamydia infection. For this, urine samples were collected and analyzed using the Polymerase Chain Reaction Technique (PCR). Results: C. trachomatis was detected in 4.2% of the total 117 HIV patients. Out of positive cases 60% were males and 40% were females. However, chlamydia was found more prevalent among females (6.8%) than males (3.4%). Eighty percent of positive cases were asymptomatic. Conclusions: Although, the prevalence of chlamydia infection was found less HIV patients, most of those cases were asymptomatic. Therefore, routine checkup is recommended for all suspected cases for timely management of the disease. DOI: http://doi.dx.org/10.3126/ijim.v2i1.8003 Int J Infect Microbiol 2013;2(1):12-16

Homelessness, sex and a tale of two sexually transmitted infections

2020 ◽  
pp. 095646242095890
Author(s):  
D Henning ◽  
C Parrott ◽  
T Read ◽  
S Cook ◽  
CS Bradshaw
Keyword(s):  
Mycoplasma Genitalium ◽  
High Risk Population ◽  
Specialist Care ◽  
Transmitted Infection ◽  
Care Clinic ◽  
Management Guidelines ◽  
Sexually Transmitted ◽  
Screening Practice ◽  
First Pass ◽  
Polymerase Chain

The Young People’s Health Service (YPHS) is a free, nurse-led Primary Health Care Clinic, in Melbourne, for young people aged 12–24 who are experiencing homelessness. Sexually transmitted infection (STI) screening is routinely offered as part of comprehensive psychosocial assessments. We wanted to determine the number of people positive for Chlamydia trachomatis (Ct) and Mycoplasma genitalium (Mg), amongst this asymptomatic high-risk population. We also wanted to review our screening practice. All asymptomatic sexually active clients seen by YPHS between 2014 and 2016 were offered a first pass urine polymerase chain reaction-based test for Ct and Mg. Urine samples were taken for men and women. Positivity for Ct and Mg out of those tested was determined and association with gender examined. Between 2014–2016, 272 males and 278 females (n = 550) were screened for Ct, and 72 infections were detected (13.1%. Chlamydia positivity did not differ between males (n = 35; 12.9%, 95% confidence interval [CI]: 8.8–16.8) and females (n = 37; 13.3%, 95%CI: 9.3–17.3). Over the same period 273 males and 284 females were screened for Mg (n = 557) and 55 infections were detected (9.9%). A higher proportion of females (n = 35; 12.3%, 95%CI: 8.5–16.1) tested positive compared to males (n = 20; 7.3%, 95%CI: 4.2–10.4), p = 0.048. Our study demonstrates both Ct and Mg are prevalent in the population, Mg being more common in young women than young men. Referral for specialist care for macrolide-resistant Mg increased and the updated Australian STI management guidelines led to a review of practice.

Detection of male genital infection with Chlamydia trachom atis and Neisseria gonorrhoeae using an automated multiplex PCR system (Cobas AmplicorTM)

1998 ◽  
Vol 9 (1) ◽  
pp. 21-24 ◽  
Author(s):  
Stephen P Higgins ◽  
Paul E Klapper ◽  
J Keith Struthers ◽  
Andrew S Bailey ◽  
Alison P Gough ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Dot Blot ◽  
Chain Reaction ◽  
Transmitted Infection ◽  
Sexually Transmitted ◽  
Primary Care Settings ◽  
Highly Sensitive ◽  
Significant Difference ◽  
Polymerase Chain ◽  
Male Genital

Summary: We evaluated Cobas AmplicorTM, a highly automated polymerase chain reaction (PCR) system, to test first-void urine (FVU) and urethral swab specimens for Chlamydia trachomatis and Neisseria gonorrhoeae in men attending a sexually transmitted infection (STI) clinic. Results were compared against an inhouse radioimmune dot blot (DB) test for C. trachomatis and selective culture for N. gonorrhoeae . Three hundred and ninety sets of specimens were obtained from 378 consecutive new and returned-new patients. Gonorrhoea prevalence was 9.49%, with no significant difference in sensitivity or specificity between culture and PCR. Chlamydia prevalence was 15.4%, with sensitivities of: DB 55%, PCR of FVU 86.7%, urethral swab PCR 90%. The specificity of PCR on FVU and urethral swabs was 100%. We have shown that Cobas AmplicorTM PCR is highly sensitive and specific in the diagnosis of chlamydia and gonorrhoea in men attending an STI clinic. Further economic and scientific studies are needed to determine the costeffectiveness of this technique for screening in primary care settings.

Prevalence of Mycoplasma genitalium in men with gonococcal urethritis

1996 ◽  
Vol 7 (6) ◽  
pp. 443-444 ◽  
Author(s):  
M Uno ◽  
T Deguchi ◽  
H Komeda ◽  
M Yasuda ◽  
M Tamaki ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Enzyme Immunoassay ◽  
Mycoplasma Genitalium ◽  
Chain Reaction ◽  
Gonococcal Urethritis ◽  
Japanese Male ◽  
Male Patients ◽  
Polymerase Chain

A significant association of Mycoplasma genitalium with non-gonococcal urethritis has been reported, but the prevalence of this mycoplasma in men with gonococcal urethritis has not been so well studied. In this study, we examined urethral swab specimens from 45 Japanese male patients with gonococcal urethritis for the presence of M. genitalium by using a polymerase chain reaction-based assay. We also sought Chlamydia trachomatis by an enzyme immunoassay Chlamydiazyme . Of the 45 specimens, 2 4.4 were positive for the mycoplasma and 12 26.7 were positive for C. trachomatis. The findings suggest that M. genitalium may be a cause not only of non-gonococcal urethritis but also of postgonococcal urethritis.

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