scholarly journals Comparative Evaluation of Rose Bengal Plate Agglutination Test, Mallein Test, and Some Conventional Serological Tests for Diagnosis of Equine Glanders

2007 ◽  
Vol 19 (4) ◽  
pp. 362-367 ◽  
Author(s):  
Abeera Naureen ◽  
Muhammad Saqib ◽  
Ghulan Muhammad ◽  
Muhammad H. Hussain ◽  
Muhammad N. Asi
Keyword(s):  
Rose Bengal ◽  
Agglutination Test ◽  
Diagnostic Efficiency ◽  
Serological Tests ◽  
Predictive Values ◽  
Indirect Hemagglutination ◽  
Indirect Hemagglutination Test ◽  
The Rose ◽  
Culture Positive ◽  
Supplementary Test

The Rose Bengal plate agglutination test (RBT) was evaluated for the diagnosis of equine glanders, and its diagnostic efficiency was compared with that of mallein and other serological tests, including indirect hemagglutination test (IHAT), complement fixation test (CFT), and modified counter immunoelectrophoresis test (mCIET). Sera from 70 naturally infected culture-positive, 96 potentially exposed cohorts, and 110 healthy equines were tested. All tests but mCIET showed 100% specificity when testing the sera from glanders-negative equines. The calculated sensitivities of RBT, IHAT, CFT, mCIET, and mallein test when testing culture-positive equines were 90.0, 97.1, 91.4, 81.4, and 75.7%, respectively. The RBT was significantly ( P < 0.05) more sensitive than the mallein test and mCIET. The positive and negative predictive values of each test (RBT, IHAT, CFT, mallein test, and mCIET) were as follows: 100 and 94, 100 and 98.2, 100 and 96.7, 100 and 86.6, and 90.5 and 88.6, respectively. On comparing glandered and nonglandered animals, the highest agreement (0.987) was found between RBT and CFT followed by RBT and IHAT (0.940), RBT and mallein test (0.871), and RBT and mCIET (0.852). Because the RBT is simpler and rapid to perform, the inclusion of the test as a supplementary test for the diagnosis of glanders in field conditions is recommended.

scholarly journals Comparison of the results of some serological tests for bovine brucellosis

1978 ◽  
Vol 80 (3) ◽  
pp. 365-371 ◽  
Author(s):  
R. J. Chappel ◽  
D. J. McNaught ◽  
J. A. Bourke ◽  
G. S. Allan
Keyword(s):  
Rose Bengal ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Agglutination Test ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Plate Test ◽  
Comparison Of The Results ◽  
The Rose ◽  
Serum Agglutination

SummaryA total of 1887 bovine sera positive to the Rose Bengal plate test were subjected to other serological tests for bovine brucellosis: the complement fixation test using warm fixation (CFTW), the serum agglutination test (SAT) and the radioimmunoassay (RIA).The SAT was generally much less sensitive than the CFTW. Many sera, however, gave positive reactions in the SAT but no reaction in the CFTW or the RIA. These SAT reactions were attributed to IgM antibody.Comparison between the results of the CFTW and the RIA led to the conclusion that 200 ng could be used as a minimum diagnostic reaction in the RIA.

scholarly journals A quantitative comparison of the sensitivity of serological tests for bovine brucellosis to different antibody classes

1976 ◽  
Vol 76 (2) ◽  
pp. 287-298 ◽  
Author(s):  
G. S. Allan ◽  
R. J. Chappel ◽  
P. Williamson ◽  
D. J. McNaught
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Quantitative Comparison ◽  
Agglutination Test ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Plate Test ◽  
Specific Antibodies ◽  
The Rose ◽  
Serum Agglutination

SUMMARYBrucella-specific antibodies of different immunoglobulin classes were quantitatively evaluated with respect to their efficiency in serological tests for bovine brucellosis.IgM reacted more efficiently than IgG1and IgG2in both the Rose Bengal plate test and serum agglutination test. The complement fixation test was found to be slightly more sensitive to IgM than to IgG1and did not react to IgG2.IgM was, however, partly inactivated when heated at 60°C. in the presence of serum.

scholarly journals Bayesian evaluation of three serological tests for the diagnosis of bovine brucellosis in Bangladesh

2019 ◽  
Vol 147 ◽  
Author(s):  
A. K. M. A. Rahman ◽  
S. Smit ◽  
B. Devleesschauwer ◽  
P. Kostoulas ◽  
E. Abatih ◽  
...  
Keyword(s):  
Latent Class ◽  
Latent Class Model ◽  
Dairy Farm ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bovine Brucellosis ◽  
Class Model ◽  
Serological Tests ◽  
Predictive Values ◽  
Conditional Dependence

AbstractWe evaluated the performance of three serological tests – an immunoglobulin G indirect enzyme linked immunosorbent assay (iELISA), a Rose Bengal test and a slow agglutination test (SAT) – for the diagnosis of bovine brucellosis in Bangladesh. Cattle sera (n = 1360) sourced from Mymensingh district (MD) and a Government owned dairy farm (GF) were tested in parallel. We used a Bayesian latent class model that adjusted for the conditional dependence among the three tests and assumed constant diagnostic accuracy of the three tests in both populations. The sensitivity and specificity of the three tests varied from 84.6% to 93.7%, respectively. The true prevalences of bovine brucellosis in MD and the GF were 0.6% and 20.4%, respectively. Parallel interpretation of iELISA and SAT yielded the highest negative predictive values: 99.9% in MD and 99.6% in the GF; whereas serial interpretation of both iELISA and SAT produced the highest positive predictive value (PPV): 99.9% in the GF and also high PPV (98.9%) in MD. We recommend the use of both iELISA and SAT together and serial interpretation for culling and parallel interpretation for import decisions. Removal of brucellosis positive cattle will contribute to the control of brucellosis as a public health risk in Bangladesh.

The rose bengal plate agglutination test in the diagnosis of brucellosis

1969 ◽  
Vol 85 (23) ◽  
pp. 636-641 ◽  
Author(s):  
WJ Morgan ◽  
DJ MacKinnon ◽  
JR Lawson ◽  
GA Cullen
Keyword(s):  
Rose Bengal ◽  
Agglutination Test ◽  
The Rose

scholarly journals Detection of Brucella Antibodies of Sheep in Al-Anbar Province by Using Some Serological Tests

2013 ◽  
Vol 37 (1) ◽  
pp. 7-12
Author(s):  
Ekram A. Al-Samarrae
Keyword(s):  
Rose Bengal ◽  
Agglutination Test ◽  
Infected Sheep ◽  
Serological Tests ◽  
Blood Samples ◽  
Plate Test ◽  
Sheep Blood ◽  
Total Percentage ◽  
Different Ages

An investigation was carried out to detect anti-Brucella antibodies of sheep in Al-Anbar province by using few serological tests; rose Bengal plate test (RBPT) and tube agglutination test (TAT). A total of 754 sheep blood samples (94 Ram and 660 Ewe) were collected randomly distributed in Al-Anbar province from unvaccinated sheep flocks with different ages. A total percentage of infected sheep depending on RBPT was 10.21% with significant differences at (P< 0.01), it was 9.69% in ewes, whereas in rams it elevated to reach 13.82%, in addition to the disease was higher seroprevalence at > 3 years (12.78%) in both gender with significant differences at (P<0.05) than lower ages. Out of 77 RBPT positive sera, 38(49.35%) were positive using TAT, there was 14 seropositive with significant differences at (P< 0.01). The study reported that brucellosis was distributed in Al-Anbar province with variable percent (3.26% to 21.27%), as well as emphasized on different epidemiological aspects.

scholarly journals Production testing of antigenicity and immunogenicity of bivalent inactivated vaccine salmonellosis vaccine

2019 ◽  
Vol 21 (96) ◽  
pp. 28-32
Author(s):  
O. P. Boiko ◽  
O. M. Sen ◽  
B. M. Kurtiak ◽  
M. S. Romanovych ◽  
T. O. Pundiak ◽  
...  
Keyword(s):  
Surface Antigens ◽  
Breeding Birds ◽  
Agglutination Test ◽  
Production Testing ◽  
Effective Measure ◽  
Significant Difference ◽  
Indirect Hemagglutination ◽  
Avian Origin ◽  
Indirect Hemagglutination Test ◽  
Production Conditions

Scientists of both human and veterinary medicine combine their potential to develop new or improve old instruments in order to rein the problem of foodborne salmonellosis in Ukraine. According to the experience of European poultry industry, the most effective measure for the prevention of avian salmonellosis is total vaccination against salmonellosis of laying hens and breeding birds. In our country, the epizootic situation of salmonellosis of animals and poultry is consistently favorable. At the same moment nearly 90% of foods born Salmonella outbreaks are of poultry and egg products origin. In other words the source of major source of Salmonella agent is avian origin. Currently no vaccines of native origin have been registered in Ukraine. Although there have been numerous attempts to develop a vaccine against avian salmonellosis. The purpose of our work is to evaluate the antigenicity and immunogenicity of the two experimental series of bivalent inactivated emulsified vaccine against avian salmonellosis in production conditions. During the examination of the vaccine in the poultry farm it was found that for 21 days after the re-introduction of the vaccine, the titers of antibodies to the mono-antigens S. Typhimurium, Enteritidis and Gallinarum in the Agglutination Test (AT) and Indirect Hemagglutination Test (IH) were: 1 : 640–1280 to 1 : 2560–5120 respectively. This indicates high antigenicity of the vaccine. No significant difference between the levels of antibodies to Typhimurium and Enteritidis antigens was detected neither in AT nor IH. At the same time the levels of antibodies to mono-antigen Gallinarum were markedly lower in both reactions (1 : 160–1 : 320 – in AT and 1 : 320–1 : 1280 – in IH), but high enough to indicate that the vaccine creates a tense cross-humoral immunity to Salmonella surface antigens of Gallinarum serovar. The results of study of immunogenicity of the vaccine show that the vaccine is highly immunogenic. It means that after control infection of vaccinated hens none of tested Salmonella strains (S. Typhimurium and S. Enteritidis) were isolated from any organs, whereas in the control non-vaccinated group of birds both Salmonella test-strains were isolated from all organs. The obtained results provide a basis for further phases of the vaccine estimating followed by its registration in the prescribed manner.

scholarly journals Development of new antigens for serologic diagnosis of animal trypanosomosis and their use for epizootic monitoring

2021 ◽  
Vol 15 (1) ◽  
pp. 71-78
Author(s):  
Ch. Georgiou
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Test System ◽  
Fixation Test ◽  
Federal State ◽  
State Budget ◽  
Serological Tests ◽  
Hemagglutination Test ◽  
Indirect Hemagglutination ◽  
Indirect Hemagglutination Test

The purpose of the research is developing a method for obtaining erythrocyte antigens containing and not containing Trypanosoma equiperdum and T. evansi DNA, which can later be used in serological reactions to differentiate these types of Trypanosoma.Materials and methods. The studies were conducted in the Protozoology Laboratory and the Vyshnevolotsk Branch of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV RAS”, as well as livestock farms of the Russian Federation and other countries using clinical, microscopic, hematological, parasitological, biomolecular and serological methods.Results and discussion. Studies carried out for the first time have shown that it is possible to use erythrocyte antigens containing the T. equiperdum and T. evansi DNA obtained after 3-fold administration to mice and rabbits of a mixture of trypanosomal antigen with addition of 1.0 ml of an adjuvant (aluminum hydroxide), and bleeding of animals at 25 to 30 days. The formed precipitate was used as an antigen for serological tests. Experiments have shown that blood for preparation of positive serum can be taken when antibodies are in titers of 1:20 in the Prolonged Complement Fixation Test, and at least 1:400 in the Indirect Hemagglutination Test and ELISA, and for negative serum when horse blood serum reacts negatively with antigens of T. equiperdum and T. evansi in the Prolonged Complement Fixation Test, Indirect Hemagglutination Test and ELISA. The test systems of the Prolonged Complement Fixation Test, Indirect Hemagglutination Test and ELISA prepared by us with antigens containing and not containing T. equiperdum and T. evansi DNA resulted in creating a universal test system (Indirect Hemagglutination Test) for differentiating T. equiperdum from T. evansi.

scholarly journals Comparison of the Rose Bengal Plate and the Complement Fixation Tests with the Tube Agglutination Test for Diagnosis of Human Brucellosis

2017 ◽  
Vol 07 (03) ◽  
pp. 73-82 ◽  
Author(s):  
Ying-Hock Teng ◽  
Jung-Jing Teng ◽  
Samantha Chao ◽  
Hsu Chao ◽  
Suryakant D. Waghela
Keyword(s):  
Rose Bengal ◽  
Complement Fixation ◽  
Agglutination Test ◽  
Human Brucellosis ◽  
The Rose
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