Evaluation of an Ovine Testis Cell Line (OA3.Ts) for Propagation of Capripoxvirus Isolates and Development of an Immunostaining Technique for Viral Plaque Visualization
2007 ◽
Vol 19
(5)
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pp. 486-491
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Keyword(s):
An ovine testis cell line (OA3.Ts) was evaluated and compared with primary lamb kidney (LK) cells for its utility in capripoxvirus propagation and titration. A comparison of OA3.Ts cell growth kinetics and morphology at low (<33) and high (34−36) passage levels indicated a difference in both characteristics. However, viral titers determined in low and high passage OA3.Ts cells were comparable with those obtained using LK cells. Capripoxvirus infection of OA3.Ts and LK cells resulted in a similar cytopathic effect, which allowed for the detection of discrete viral plaques following immunostaining with capripoxvirus-specific antiserum.
1999 ◽
Vol 65
(2)
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pp. 121-132
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Keyword(s):
1996 ◽
Vol 74
(2)
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pp. 301-307
Keyword(s):
1999 ◽
Vol 29
(5)
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pp. 852-862
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Keyword(s):
2009 ◽
Vol 17
(9)
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pp. 719-723
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Keyword(s):