scholarly journals Pathology of coccidioidomycosis in llamas and alpacas

2018 ◽  
Vol 30 (4) ◽  
pp. 560-564 ◽  
Author(s):  
Julian A. Fernandez ◽  
Mireia N. Hidalgo ◽  
Emir Hodzic ◽  
Santiago S. Diab ◽  
Francisco A. Uzal
Keyword(s):  
Food Safety ◽  
Animal Health ◽  
Large Percentage ◽  
Anecdotal Evidence ◽  
Coccidioides Immitis ◽  
Coccidioides Posadasii ◽  
Pcr Assay ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

Coccidioidomycosis is a fungal disease caused by either Coccidioides immitis or Coccidioides posadasii. Anecdotal evidence suggests that camelids are particularly susceptible to this disease and that a relatively large percentage of pneumonias in these animals are caused by Coccidioides spp. In a search of 21 y (1992–2013) of records from the California Animal Health and Food Safety Laboratory, we found 79 cases of coccidioidomycosis diagnosed in camelids; 66 (84%) had pneumonia and 13 (16%) had lesions only in organs other than the lungs. The organs most frequently affected were lung (84%) and liver (78%). Coccidioides spp. were the cause of pneumonia in 66 of 362 (18%) camelid cases during the study period. The lesions in affected organs were multifocal-to-coalescing pyogranulomas, which in most cases were visible grossly. Ten of the 12 formalin-fixed, paraffin-embedded lung samples tested by a universal Coccidioides spp. PCR assay were positive (4 C. immitis, 2 C. posadasii); the species could not be determined in 4 of the 10 cases positive by PCR. Coccidioidomycosis is an important cause of pneumonia in camelids in California, and can be caused by either C. immitis or C. posadasii.

scholarly journals Molecular identification of Coccidioides immitis in formalin-fixed, paraffin-embedded (FFPE) tissues from a Colombian patient

2015 ◽  
Vol 53 (5) ◽  
pp. 520-527 ◽  
Author(s):  
Cristina E. Canteros ◽  
Alejandro Vélez H. ◽  
Adriana I. Toranzo ◽  
Roberto Suárez-Alvarez ◽  
Ángela Tobón O. ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Coccidioides Immitis ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Ffpe Tissues ◽  
Formalin Fixed

scholarly journals Immunohistochemical Detection of Porcine Reproductive and Respiratory Syndrome Virus Antigen in Formalin-Fixed, Paraffin-Embedded Tissues with Correlation to Clinicopathologic Data

2017 ◽  
Vol 3 (6) ◽  
pp. 252
Author(s):  
Lavina Gracia G. Manzano
Keyword(s):  
Animal Health ◽  
Virus Antigen ◽  
The Philippines ◽  
Respiratory Syndrome Virus ◽  
Tissue Samples ◽  
Follicular Hyperplasia ◽  
Prrs Virus ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

Immunohistochemistry (IHC) was used in this study to detect the presence of porcine reproductive and respiratory virus (PRRSV) antigen using monoclonal antibody (Mab SDOW17) in formalin-fixed, paraffin embedded tissues of lung, lymph node, heart, spleen, and kidney of pre- weaning to less than 90 day old pigs. Out of the 25 tissue samples examined from swine cases of the Philippine Animal Health Center (PAHC), Bureau of Animal Industry and College of Veterinary Medicine (CVM) Histopathology Laboratory, University of the Philippines-Los Baños (UPLB), 14 or 56% (14/25) were IHC positive for PRRSV antigen.  Among the selected tissue samples, the PRRS virus was detected the most in the lymph nodes (64%) and lungs (40%), respectively, compared to other organs such as spleen (33%), kidney (28%), and the heart (17%). Only 43% (6/14) of cases coincided with the pathogenesis and clinicopathologic lesions of PRRS which are proliferative interstitial pneumonia and lymphoid follicular hyperplasia and necrosis. PRRS positive cases in IHC were consistently found to have co-infections with viral and bacterial diseases. Since PRRSV has tropism for macrophages and destruction of these cells leads to immunosuppression, affected animals are vulnerable to secondary infections. Keywords: formalin-fixed;  immunohistochemistry;  paraffin embedded tissue;  piglets;  Porcine Reproductive and Respiratory Syndrome; PRRS virus antigen; Mab SDOW17 

Detection of Naegleria fowleri, Acanthamoeba spp, and Balamuthia mandrillaris in Formalin-Fixed, Paraffin-Embedded Tissues by Real-Time Multiplex Polymerase Chain Reaction

2019 ◽  
Vol 152 (6) ◽  
pp. 799-807 ◽  
Author(s):  
Andrew P Norgan ◽  
Lynne M Sloan ◽  
Bobbi S Pritt
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Limit Of Detection ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Ffpe Tissues ◽  
Polymerase Chain ◽  
Formalin Fixed

Abstract Objectives Pathogenic free-living amebae (FLAs) cause skin, ocular, and central nervous system (CNS) infections with significant morbidity and mortality. Diagnosis of FLA infections by pathologic examination of tissue sections can be aided using molecular assays. This study investigated the performance characteristics of a multiplex real-time polymerase chain reaction (PCR) assay (FLA-PCR) for detection and differentiation of FLAs in clinical specimens. Methods FLA-PCR was performed on 39 human specimens comprising one cutaneous, 14 corneal, and 24 CNS formalin-fixed, paraffin-embedded (FFPE) tissues with a histopathologic diagnosis of FLA infection and four CNS FFPE tissues with inflammation but no evidence of FLAs. In addition, clinical specificity and assay limit of detection were determined. Results FLA detection sensitivities ranged from 79% to 84% in FFPE tissues. No cross-reactivity was observed. Conclusions While sensitivity is limited, FLA-PCR assay may serve as a useful adjunct for detection or confirmation of FLA infections in FFPE tissues.

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