Serum Allergen Specific Immunoglobulin E Levels in Patients with Allergic Conjunctivitis

Aim: To determine the differences between total and serum-specific immunoglobulin E levels in patients with type 1 allergic conjunctivitis and asymptomatic contact lens wearers. The correlation of total serum immunoglobulin E level of asymptomatic contact lens wearers with contact lens wearing time, and total duration of contact lens use was also evaluated.Methods: This was a case-control study involving 25 asymptomatic contact lens wearers, 25 patients with type 1 allergic conjunctivitis, and 25 age- and sex-matched healthy controls. Total serum immunoglobulin E levels were detected by enzyme-linked immunosorbent assay. Serum-specific IgE analysis against the listed indoor, food, and outdoor allergens were studied by immunofluorescence assay for participants whose total serum immunoglobulin E levels were >100 IU/mL. Pearson’s and Spearman’s correlations were used for bivariate analysis. Statistical significance was accepted at the 0.05 level.Results: The mean level of total serum immunoglobulin E was greater for patients with type 1 allergic conjunctivitis than for contact lens wearers and controls. Serum-specific immunoglobulin E detected in patients with type 1 allergic conjunctivitis was against indoor, food, and outdoor allergens, while serum-specific immunoglobulin E detected in contact lens wearers was only against outdoor allergens. A statistically significant correlation was found for total serum immunoglobulin E levels of contact lens wearers with contact lens wearing time.Conclusions: These results suggest that differences in serum total and specific immunoglobulin E levels exist between patients with type 1 allergic conjunctivitis and CL wearers and controls. Further research in a larger group of patients is needed to validate these findings.

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Optimal cutoff values of allergen-specific immunoglobulin E to house dust mites and animal dander based on skin-prick test results: Analysis in 16,209 patients with allergic rhinitis

American Journal of Rhinology and Allergy ◽

10.2500/ajra.2017.31.4483 ◽

2017 ◽

Author(s):

S. D. Hong ◽

G. Ryu ◽

M. Y. Seo ◽

J. I. Jeong ◽

H. Y. Kim ◽

...

Keyword(s):

Allergic Rhinitis ◽

Immunoglobulin E ◽

House Dust Mites ◽

Test Results ◽

Dust Mites ◽

Optimal Cutoff ◽

Prick Test ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin ◽

Animal Dander

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Allergic diseases attributable to atopy in a population sample of Asian children

Scientific Reports ◽

10.1038/s41598-021-95579-2 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Chao-Yi Wu ◽

Hsin-Yi Huang ◽

Wen-Chi Pan ◽

Sui-Ling Liao ◽

Man-Chin Hua ◽

...

Keyword(s):

Immunoglobulin E ◽

Population Attributable Risk ◽

Population Sample ◽

International Study ◽

Allergic Diseases ◽

Population Based ◽

School Age Children ◽

Specific Immunoglobulin E ◽

Asian Children ◽

Specific Immunoglobulin

AbstractThe proportion of allergic diseases attributable to atopy remains a subject of controversy. This study aimed to estimate the population risk of physician-diagnosed asthma, rhinitis and eczema attributed to atopy among a population sample of Asian school-age children. Asian children aged 5–18 years (n = 1321) in the Prediction of Allergies in Taiwanese CHildren (PATCH) study were tested for serum allergen-specific immunoglobulin E. Physician-diagnosed asthma, rhinitis and eczema were assessed by a modified International Study of Asthma and Allergies in Childhood questionnaire. Atopy was defined as the presence of serum allergen-specific immunoglobulin E. In this population-based study, 50.4% of the subjects with asthma, 46.3% with rhinitis, and 46.7% with eczema were attributable to atopy. The population attributable risk (PAR) of atopy for three allergic diseases was higher in adolescents (asthma, 54.4%; rhinitis, 59.6%; eczema, 49.5%) than younger children aged less than 10 years (asthma, 46.9%; rhinitis, 39.5%; eczema, 41.9%). Among the seven allergen categories, sensitization to mites had the highest PARs for all three allergic diseases (51.3 to 64.1%), followed by sensitization to foods (asthma, 7.1%; rhinitis, 10.4%; eczema 27.7%). In conclusion, approximately half (46.3 to 50.4%) of Asian children in Taiwan with allergic diseases are attributable to atopy.

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Effect of Specific Immunoglobulin E Response and Comorbidities on Effectiveness of MP-AzeFlu in a Real-Life Study

International Archives of Allergy and Immunology ◽

10.1159/000508749 ◽

2020 ◽

Vol 181 (10) ◽

pp. 754-764

Author(s):

Ludger Klimek ◽

David Price ◽

Gabriella Gálffy ◽

Melanie Emmeluth ◽

Arkady Koltun ◽

...

Keyword(s):

Immunoglobulin E ◽

Real Life ◽

Life Study ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin ◽

Real Life Study

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Total and Specific Immunoglobulin E and Their Relationship to Respiratory Symptoms in Quebec Children and Adolescents

Canadian Respiratory Journal ◽

10.1155/2005/248714 ◽

2005 ◽

Vol 12 (8) ◽

pp. 426-432 ◽

Cited By ~ 6

Author(s):

Benoît Levesque ◽

Jean-François Duchesne ◽

Suzanne Gingras ◽

Pierre Allard ◽

Edgar Delvin ◽

...

Keyword(s):

Children And Adolescents ◽

Respiratory Symptoms ◽

Immunoglobulin E ◽

Geometric Mean ◽

Allergic Sensitization ◽

Population Sample ◽

Ethnic Origin ◽

Total Ige ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin

BACKGROUND: Respiratory disease is a major cause of morbidity in young people. It is now recognized that atopy plays an important role in the development of chronic respiratory symptoms in children.OBJECTIVE: To examine the determinants and consequences of serum total and specific immunoglobulin E (IgE) in a general population sample of Québec children and adolescents.METHODS: In 1999, 2349 children and adolescents (nine, 13 or 16 years of age) who had participated in a respiratory symptom and disease questionnaire had their total IgE measured. Of these participants, a subsample of 451 children and adolescents was analyzed to detect antibodies to eight specific allergens (ie, allergens of dust mites [Dermatophagoides farinae and Dermatophagoides pteronyssinus], cat, dog, ragweed, Timothy grass, mould [alternaria] and cockroach).RESULTS: The geometric mean of the total IgE was 44.4 U/mL among all participants. Concentrations were higher in boys and increased with age. More than 41% of the participants were sensitized to at least one specific allergen. Such sensitization was strongly associated with the occurrence of respiratory conditions and symptoms, namely asthma, wheezing and rhinitis. Family history, school location and ethnic origin had an impact on the prevalence of atopy and total IgE levels.CONCLUSIONS: Allergic sensitization is a major determinant in the development of asthma, wheezing and rhinitis in children and adolescents in the province of Québec.

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A Simple and Rapid Light-Initiated Chemiluminescence Assay for Quantitation of Artemisia-Specific Immunoglobulin E

International Archives of Allergy and Immunology ◽

10.1159/000520511 ◽

2021 ◽

pp. 1-8

Author(s):

Dandan Liu ◽

Bei Zhang ◽

Lina Zhu ◽

Lisheng Zheng ◽

Shaoshen Li ◽

...

Keyword(s):

Food Allergen ◽

Clinical Guideline ◽

Immunoglobulin E ◽

Limit Of Detection ◽

Enzyme Linked Immunosorbent Assay ◽

Serum Samples ◽

Chemiluminescence Assay ◽

Limit Of Quantitation ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin

Background: Light-initiated chemiluminescence assay (LICA) is a homogeneous assay that has been successfully used for the quantitation of food allergen-specific immunoglobulin E (sIgE), but not inhaled allergen-sIgE. Simultaneously, current assays used to detect allergen-sIgE are serum consuming and/or time consuming. Hence, we established a method for the quantitation of Artemisia-sIgE based on LICA and verified its performance according to the clinical guideline documents, laying a foundation for the quantitation of inhaled and food allergen-sIgE in parallel on LICA. Methods: The assay was established after optimizing the first incubation time and the dilutions of Artemisia-coated chemibeads, biotinylated goat anti-human IgE, and serum. In order to quantitate Artemisia-sIgE, the calibration curve was established with a high positive serum of known concentration. The assay performance was confirmed per the clinical guideline documents. In addition, the correlation between the results of LICA and capture enzyme-linked immunosorbent assay was evaluated. Results: The developed LICA’s coefficients of variation of repeatability and intermediate precision were 3.20%, 2.14%, and 3.85% and 4.30%, 4.00%, and 4.40%, respectively. The limit of detection was 0.10 kUA/L, and the limit of quantitation was 0.11 kUA/L. The range of linearity was from 0.27 kUA/L to 97.53 kUA/L (r = 0.9968). The correlation coefficient (r) for the correlation analysis between results of LICA and capture ELISA was 0.9087. This assay was successfully applied in 64 human serum samples, showing good sensitivity (82.20%) and specificity (100%). Conclusion: An Artemisia-sIgE quantitation assay based on LICA was successfully established. Its performance satisfied the clinical requirements and could be widely used in clinical laboratories.

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