scholarly journals Serum Immunoglobulin E Levels in Patients with Allergic Conjunctivitis and Contact Lens Wearers

1970 ◽  
Vol 12 (3) ◽  
pp. 152-155
Author(s):  
Ozlem Gurses Sahin ◽  
Nusret Taheri
Keyword(s):  
Contact Lens ◽  
Allergic Conjunctivitis ◽  
Immunoglobulin E ◽  
Total Duration ◽  
Serum Immunoglobulin ◽  
Total Serum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin

Aim: To determine the differences between total and serum-specific immunoglobulin E levels in patients with type 1 allergic conjunctivitis and asymptomatic contact lens wearers. The correlation of total serum immunoglobulin E level of asymptomatic contact lens wearers with contact lens wearing time, and total duration of contact lens use was also evaluated.Methods: This was a case-control study involving 25 asymptomatic contact lens wearers, 25 patients with type 1 allergic conjunctivitis, and 25 age- and sex-matched healthy controls. Total serum immunoglobulin E levels were detected by enzyme-linked immunosorbent assay. Serum-specific IgE analysis against the listed indoor, food, and outdoor allergens were studied by immunofluorescence assay for participants whose total serum immunoglobulin E levels were >100 IU/mL. Pearson’s and Spearman’s correlations were used for bivariate analysis. Statistical significance was accepted at the 0.05 level.Results: The mean level of total serum immunoglobulin E was greater for patients with type 1 allergic conjunctivitis than for contact lens wearers and controls. Serum-specific immunoglobulin E detected in patients with type 1 allergic conjunctivitis was against indoor, food, and outdoor allergens, while serum-specific immunoglobulin E detected in contact lens wearers was only against outdoor allergens. A statistically significant correlation was found for total serum immunoglobulin E levels of contact lens wearers with contact lens wearing time.Conclusions: These results suggest that differences in serum total and specific immunoglobulin E levels exist between patients with type 1 allergic conjunctivitis and CL wearers and controls. Further research in a larger group of patients is needed to validate these findings.

scholarly journals Faecal eosinophil cationic protein and serum immunoglobulin E in relation to infant feeding practices

2016 ◽  
Vol 54 (2) ◽  
pp. 246-252 ◽  
Author(s):  
Man-Chin Hua ◽  
Chien-Chang Chen ◽  
Sui-Ling Liao ◽  
Tsung-Chieh Yao ◽  
Ming-Han Tsai ◽  
...  
Keyword(s):  
Intestinal Inflammation ◽  
Immunoglobulin E ◽  
Eosinophil Cationic Protein ◽  
Serum Immunoglobulin ◽  
Breastfeeding Duration ◽  
Total Serum ◽  
Cationic Protein ◽  
Solid Foods ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin

Background To date, the effects of exclusive breastfeeding duration and timing of solid food introduction on allergy prevention are unclear. The aim of this study was to determine the effect of variable feeding practices on intestinal inflammation in infants using faecal eosinophil cationic protein as a surrogate marker and to assess whether faecal eosinophil cationic protein is associated with serum immunoglobulin E. Methods Subjects ( n = 206) were enrolled from the Prediction of Allergies in Taiwanese CHildren (PATCH) birth cohort study. Stool samples were collected at 6 and 12 months for determining eosinophil cationic protein, and blood was collected for determining total and allergen-specific immunoglobulin E at 12 months. We compared these biomarkers between infants with variable exclusive breastfeeding duration and infants introduced to solid foods at various periods. The association between faecal eosinophil cationic protein, total serum immunoglobulin E and specific immunoglobulin E was also analysed. Results Faecal eosinophil cationic protein was significantly higher in exclusively breastfed infants compared with formula-fed infants and infants who were not exclusively breastfed at 6 months of age ( P < 0.05). At 12 months, infants who were introduced to solid foods at 5–6 months had the lowest faecal eosinophil cationic protein compared with those who were introduced at earlier and later periods. There was no significant association between faecal eosinophil cationic protein and serum immunoglobulin E. Conclusion We found that breastfeeding exclusively for >6 months did not reduce serum immunoglobulin E, but rather increased intestinal inflammation. Faecal eosinophil cationic protein was not associated with total serum immunoglobulin E and specific immunoglobulin E and might not be a useful indictor of immunoglobulin E sensitization in infancy.

A Simple and Rapid Light-Initiated Chemiluminescence Assay for Quantitation of Artemisia-Specific Immunoglobulin E

2021 ◽  
pp. 1-8
Author(s):  
Dandan Liu ◽  
Bei Zhang ◽  
Lina Zhu ◽  
Lisheng Zheng ◽  
Shaoshen Li ◽  
...  
Keyword(s):  
Food Allergen ◽  
Clinical Guideline ◽  
Immunoglobulin E ◽  
Limit Of Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Chemiluminescence Assay ◽  
Limit Of Quantitation ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin

<b><i>Background:</i></b> Light-initiated chemiluminescence assay (LICA) is a homogeneous assay that has been successfully used for the quantitation of food allergen-specific immunoglobulin E (sIgE), but not inhaled allergen-sIgE. Simultaneously, current assays used to detect allergen-sIgE are serum consuming and/or time consuming. Hence, we established a method for the quantitation of <i>Artemisia</i>-sIgE based on LICA and verified its performance according to the clinical guideline documents, laying a foundation for the quantitation of inhaled and food allergen-sIgE in parallel on LICA. <b><i>Methods:</i></b> The assay was established after optimizing the first incubation time and the dilutions of <i>Artemisia</i>-coated chemibeads, biotinylated goat anti-human IgE, and serum. In order to quantitate <i>Artemisia</i>-sIgE, the calibration curve was established with a high positive serum of known concentration. The assay performance was confirmed per the clinical guideline documents. In addition, the correlation between the results of LICA and capture enzyme-linked immunosorbent assay was evaluated. <b><i>Results:</i></b> The developed LICA’s coefficients of variation of repeatability and intermediate precision were 3.20%, 2.14%, and 3.85% and 4.30%, 4.00%, and 4.40%, respectively. The limit of detection was 0.10 kU<sub>A</sub>/L, and the limit of quantitation was 0.11 kU<sub>A</sub>/L. The range of linearity was from 0.27 kU<sub>A</sub>/L to 97.53 kU<sub>A</sub>/L (<i>r</i> = 0.9968). The correlation coefficient (<i>r</i>) for the correlation analysis between results of LICA and capture ELISA was 0.9087. This assay was successfully applied in 64 human serum samples, showing good sensitivity (82.20%) and specificity (100%). <b><i>Conclusion:</i></b> An <i>Artemisia</i>-sIgE quantitation assay based on LICA was successfully established. Its performance satisfied the clinical requirements and could be widely used in clinical laboratories.

Serum Allergen Specific Immunoglobulin E Levels in Patients with Allergic Conjunctivitis

2008 ◽  
Vol 18 (5) ◽  
pp. 675-679 ◽  
Author(s):  
S. Kocabeyoglu ◽  
B. Bozkurt ◽  
O. Bilen ◽  
M. Irkec ◽  
M. Orhan
Keyword(s):  
Allergic Conjunctivitis ◽  
Immunoglobulin E ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin

scholarly journals Characterization and identification of charcoal of inedible Kerandang fish (Channa pleurophthalmus Blkr) body parts and potential antiallergenic properties

2020 ◽  
Vol 13 (7) ◽  
pp. 1480-1486
Author(s):  
Aryani Aryani ◽  
Eddy Suprayitno ◽  
Bambang Budi Sasmito ◽  
Hardoko Hardoko
Keyword(s):  
Ethyl Acetate ◽  
Immunoglobulin E ◽  
High Resolution Mass Spectrometry ◽  
Ethyl Acetate Extract ◽  
The Body ◽  
Enzyme Linked Immunosorbent Assay ◽  
Body Parts ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin ◽  
Resolution Mass

Background and Aim: The study about the antiallergenic properties of inedible fish body parts is still limited. Therefore, this study aimed to characterize the charcoal from the body parts of Kerandang fish (Channa pleurophthalmus Blkr) and identify its antiallergenic properties. Materials and Methods: This study used some non-edible body parts extracted from the Kerandang fish (i.e., the scalp, scales, and dorsal, pectoral, ventral, anal, and caudal fins) using a maceration method with different solvents (ethanol, ethyl acetate, and chloroform). The identification of active compounds in the extract was carried out using liquid chromatography– high-resolution mass spectrometry (LC-HRMS) analysis, while the antihyaluronidase activity was determined using the antihyaluronidase test. The highest charcoal antihyaluronidase activity-extract was applied to ovalbumin-induced mice for 7 days with various doses (10, 15, and 20 mg/kg). The specific immunoglobulin E (IgE) was measured using enzyme-linked immunosorbent assay on day 8. Results: Our LC-HRMS analysis showed that the active compound of charcoal in the caudal fins of Kerandang fish was hexadecanamide. The highest inhibition (IC50) of hyaluronidase was found in the ethyl acetate extract of fish caudal fins at a concentration of 4 mg/mL. We found that 15 mg/kg body weight of charcoal of fish caudal fins suppressed IgE expression in male mice. Conclusion: Our findings indicate that the charcoal of non-edible body parts of Kerandang and one of its constituent, hexadecanamide, may have strong antiallergic effects.

Sign in / Sign up

Export Citation Format

Share Document