scholarly journals ELECTRON HISTOCHEMICAL LOCALIZATION OF ACID PHOSPHATASE ACTIVITY IN THE SMALL INTESTINE OF MOUSE

1964 ◽  
Vol 12 (4) ◽  
pp. 229-238 ◽  
Author(s):  
TIBOR BARKA
Keyword(s):  
Small Intestine ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Histochemical Localization

DETERMINATION OF ACID PHOSPHATASE ACTIVITY IN TISSUE HOMOGENATES

1966 ◽  
Vol 44 (6) ◽  
pp. 755-761 ◽  
Author(s):  
John P. Manning ◽  
Arthur L. Babson ◽  
Margaret C. Butler ◽  
Sara F. Priester
Keyword(s):  
Small Intestine ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Azo Dye ◽  
Accurate Method ◽  
Acid Phosphate ◽  
Fast Red ◽  
Tissue Homogenates

A simple and accurate method is described for the quantitative determination of acid phosphatase in tissue homogenates, utilizing stable buffered sodium α-naphthyl acid phosphate tablets as substrate, and the monocoupler Fast Red Salt B as the color developer. This technique eliminates deproteinization and ethyl acetate extraction, and produces a highly colored blue azo dye for spectrophotometry. Of the rat organs tested, the spleen had the highest acid phosphatase activity, then kidney, small intestine, liver, lung, and heart, in that order.

Erythrophagocytosis in the Liver in Hemolytic Anemias

1968 ◽  
Vol 26 ◽  
pp. 184-185
Author(s):  
O. T. Minick ◽  
E. Orfei ◽  
F. Volini ◽  
G. Kent
Keyword(s):  
Acid Phosphatase ◽  
Oxidative Damage ◽  
Phosphatase Activity ◽  
Kupffer Cells ◽  
Acid Phosphatase Activity ◽  
Common Type ◽  
Red Cell ◽  
Cell Fragments ◽  
Reticulo Endothelial System ◽  
Important Site

Hemolytic anemias were produced in rats by administering phenylhydrazine or anti-erythrocytic (rooster) serum, the latter having agglutinin and hemolysin titers exceeding 1:1000.Following administration of phenylhydrazine, the erythrocytes undergo oxidative damage and are removed from the circulation by the cells of the reticulo-endothelial system, predominantly by the spleen. With increasing dosage or if animals are splenectomized, the Kupffer cells become an important site of sequestration and are greatly hypertrophied. Whole red cells are the most common type engulfed; they are broken down in digestive vacuoles, as shown by the presence of acid phosphatase activity (Fig. 1). Heinz body material and membranes persist longer than native hemoglobin. With larger doses of phenylhydrazine, erythrocytes undergo intravascular fragmentation, and the particles phagocytized are now mainly red cell fragments of varying sizes (Fig. 2).

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