Laser Speckle Contrast Imaging for Intraoperative Quantitative Assessment of Intestinal Blood Perfusion During Colorectal Surgery: A Prospective Pilot Study

2019 ◽  
Vol 26 (3) ◽  
pp. 293-301 ◽  
Author(s):  
Shigehiro Kojima ◽  
Tsuguo Sakamoto ◽  
Yuko Nagai ◽  
Yutaka Matsui ◽  
Kyojiro Nambu ◽  
...  
Keyword(s):  
Pilot Study ◽  
Colorectal Surgery ◽  
Intraclass Correlation ◽  
Laser Speckle ◽  
Fluorescence Angiography ◽  
Contrast Imaging ◽  
Flow Measurements ◽  
Laser Speckle Contrast Imaging ◽  
Speckle Contrast ◽  
Perfusion Assessment

Background. The main limitation of perfusion assessment with indocyanine green fluorescence angiography during colorectal surgery is that the surgeon assesses the quality of perfusion subjectively. The ideal intestinal viability test must be minimally invasive, objective, and reproducible. We evaluated the quantitativity and reproducibility of laser speckle contrast imaging for perfusion assessment during colorectal surgery. Methods. This was a prospective, nonrandomized, pilot study of 8 consecutive patients who underwent elective left-sided colorectal resection. Laser speckle perfusion images at the site of proximal transection of the bowel were obtained intraoperatively. We tested the hypothesis that laser speckle contrast imaging was able to quantitatively identify areas of diminished intestinal perfusion after devascularization and assessed the reproducibility of this method. Results. All surgical procedures were uneventful and blood flow measurements were successfully made in all patients. None of the patients developed postoperative complications related to the anastomosis and stoma. Data analyses were successfully optimized to perform quantitative regional perfusion assessments in all cases. The bowel tissue blood flows of the anal side region adjacent to the transection line were significantly lower than those of the oral side region adjacent to the transection line after ligation of marginal vessels ( P = .012). Interrater reliability was high (intraclass correlation coefficients = 0.989), and a Bland-Altman plot showed few differences of mean flux data between 2 investigators. Conclusion. Laser speckle contrast imaging is feasible for real-time assessment of bowel perfusion with quantitativity and excellent reproducibility during colorectal surgery without administration of any contrast agents.

Laser speckle contrast imaging and quantitative fluorescence angiography for perfusion assessment

2019 ◽  
Vol 404 (4) ◽  
pp. 505-515 ◽  
Author(s):  
Jonas Hedelund Rønn ◽  
Nikolaj Nerup ◽  
Rune Broni Strandby ◽  
Morten Bo Søndergaard Svendsen ◽  
Rikard Ambrus ◽  
...  
Keyword(s):  
Laser Speckle ◽  
Fluorescence Angiography ◽  
Contrast Imaging ◽  
Laser Speckle Contrast Imaging ◽  
Speckle Contrast ◽  
Perfusion Assessment ◽  
Quantitative Fluorescence

scholarly journals Reproducibility and normalization of reactive hyperemia using laser speckle contrast imaging

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0244795
Author(s):  
Behnia Rezazadeh Shirazi ◽  
Rudy J. Valentine ◽  
James A. Lang
Keyword(s):  
Reactive Hyperemia ◽  
Intraclass Correlation ◽  
Microvascular Dysfunction ◽  
Microvascular Function ◽  
Rate Of Change ◽  
Laser Speckle ◽  
Maximal Flow ◽  
Contrast Imaging ◽  
Laser Speckle Contrast Imaging ◽  
Speckle Contrast

Background Impaired perfusion indices signal potential microvascular dysfunction preceding atherosclerosis and other cardiometabolic pathologies. Post-occlusive reactive hyperemia (PORH), a vasodilatory response following a mechanically induced ischemia, is a transient increase in perfusion and can assess microvascular function. The greatest blood flow change corresponding to the first minute of hyperemia (represented by time-to-peak, hyperemic velocity, AUC within 1st min) has been shown to indicate microvascular dysfunction. However, the reproducibility of these temporal kinetic indices of the PORH response is unknown. Our aim was to examine the inter- and intra-day reproducibility and standardization of reactive hyperemia, with emphasis on the kinetic indices of PORH, using laser speckle contrast imaging (LSCI) technique. Methods and results Seventeen healthy adults (age = 24 ± 3 years) completed three PORH bouts over two lab visits. LSCI region of interest was a standardized 10 cm region on the dominant ventral forearm. A 5-min brachial artery occlusion period induced by inflating an arm cuff to 200 mmHg, preceded a 4-min hyperemic period. Inter- and intra-day reliability and reproducibility of cutaneous vascular conductance (LSCI flux / mean arterial pressure) were determined using intraclass correlation (ICC) and coefficient of variation (CV%). Maximal flow and area under the curve standardized to zero perfusion showed intra- and inter-day reliability (ICC > 0.70). Time to maximal flow (TMF) was not reproducible (inter-day CV = 18%). However, alternative kinetic indices such as 1-min AUC and overshoot rate-of-change (ORC), represented as a piecewise function (at 5s, 10s, 15s, and 20s into hyperemia), were reproducible (CV< 11%). Biological zero was a reliable normalization point. Conclusion PORH measured with LSCI is a reliable assessment of microvascular function. However, TMF or its derived hyperemic velocity are not recommended for longitudinal assessment. Piecewise ORC and 1-min AUC are reliable alternatives to assess the kinetic response of PORH.

Intraoperative laser speckle contrast imaging for monitoring cerebral blood flow: results from a 10-patient pilot study

2012 ◽  
Author(s):  
Lisa M. Richards ◽  
Erica L. Weber ◽  
Ashwin B. Parthasarathy ◽  
Kaelyn L. Kappeler ◽  
Douglas J. Fox ◽  
...  
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Pilot Study ◽  
Laser Speckle ◽  
Contrast Imaging ◽  
Laser Speckle Contrast Imaging ◽  
Speckle Contrast

scholarly journals Nephron blood flow dynamics measured by laser speckle contrast imaging

2011 ◽  
Vol 300 (2) ◽  
pp. F319-F329 ◽  
Author(s):  
Niels-Henrik Holstein-Rathlou ◽  
Olga V. Sosnovtseva ◽  
Alexey N. Pavlov ◽  
William A. Cupples ◽  
Charlotte Mehlin Sorensen ◽  
...  
Keyword(s):  
Blood Flow ◽  
Signal Transmission ◽  
Experimental Studies ◽  
Flow Dynamics ◽  
Laser Speckle ◽  
Tubuloglomerular Feedback ◽  
Contrast Imaging ◽  
Laser Speckle Contrast Imaging ◽  
Speckle Contrast ◽  
Blood Flow Dynamics

Tubuloglomerular feedback (TGF) has an important role in autoregulation of renal blood flow and glomerular filtration rate (GFR). Because of the characteristics of signal transmission in the feedback loop, the TGF undergoes self-sustained oscillations in single-nephron blood flow, GFR, and tubular pressure and flow. Nephrons interact by exchanging electrical signals conducted electrotonically through cells of the vascular wall, leading to synchronization of the TGF-mediated oscillations. Experimental studies of these interactions have been limited to observations on two or at most three nephrons simultaneously. The interacting nephron fields are likely to be more extensive. We have turned to laser speckle contrast imaging to measure the blood flow dynamics of 50–100 nephrons simultaneously on the renal surface of anesthetized rats. We report the application of this method and describe analytic techniques for extracting the desired data and for examining them for evidence of nephron synchronization. Synchronized TGF oscillations were detected in pairs or triplets of nephrons. The amplitude and the frequency of the oscillations changed with time, as did the patterns of synchronization. Synchronization may take place among nephrons not immediately adjacent on the surface of the kidney.

scholarly journals Laser speckle contrast imaging for perfusion monitoring during surgical reconstruction of anorectal malformations

2021 ◽  
Vol 66 ◽  
pp. 101793
Author(s):  
Pernilla Stenström ◽  
Rafi Sheikh ◽  
Kristine Hagelsteen ◽  
Johanna Wennström Berggren ◽  
Malin Malmsjö
Keyword(s):  
Anorectal Malformations ◽  
Laser Speckle ◽  
Surgical Reconstruction ◽  
Contrast Imaging ◽  
Laser Speckle Contrast Imaging ◽  
Speckle Contrast
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