Investigation of a method for long-term preservation of the vascular allograft

Vascular ◽  
2021 ◽  
pp. 170853812110129
Author(s):  
Bulang He ◽  
Gabrielle Christine Musk ◽  
Zi Qin Ng ◽  
Helen Kershaw ◽  
Bastiaan DeBoer ◽  
...  
Keyword(s):  
Vascular Graft ◽  
Organ Procurement ◽  
Vena Cava ◽  
Multiple Organ ◽  
Ethanol Solution ◽  
Group V ◽  
University Of Wisconsin ◽  
Wall Calcification ◽  
Long Term Preservation

Background/objective During multiple organ procurement, blood vessels are routinely retrieved and stored in University of Wisconsin solution and then discarded after two weeks, if not used at organ transplantation owing to lack of a method for long-term preservation. Therefore, the aim of this study is to investigate a method for long-term preservation of vascular allografts in ethanol. Methods Aorta and vena cava allografts were retrieved and stored in 75% ethanol solution for 12 months at 4°C. Four pigs were divided into two groups. A segment of aorta was excised and replaced by insertion of preserved aorta graft (Group A) or vena cava graft (Group V). The pigs were observed for six weeks. A laparotomy was performed and the vascular graft was harvested for histopathology followed by euthanasia at the end of study. Results Three pigs recovered uneventfully, while one pig died from venous graft rupture in the third week after surgery. There was no aneurysmal formation or thrombosis in the grafts. Some calcification was seen over aorta allograft. On histopathology, the elastic pattern was almost normal, although the endothelial cells degenerated after preservation. After implantation, the formation of the endothelium cell-like layer was seen in both aorta and vena cava allografts. Conclusion Vascular allografts were functional after preservation for 12 months. The vena cava grafts had much less wall calcification than the aorta grafts. Further studies are necessary to investigate vascular graft remodelling with a longer observation period after implantation.

scholarly journals Hyaluronic Acid (800 kDa) Supplementation of University of Wisconsin Solution Improves Viability of Osteochondral Grafts and Reduces Matrix Metalloproteinase Expression during Cold Preservation

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Takuya Yamada ◽  
Kentaro Uchida ◽  
Kenji Onuma ◽  
Gen Inoue ◽  
Jun Aikawa ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Cell Viability ◽  
Pcr Analysis ◽  
Cartilage Defects ◽  
Osteochondral Grafts ◽  
Uw Solution ◽  
University Of Wisconsin ◽  
Long Term Preservation ◽  
Transplantation Outcomes

Osteochondral allografting is a promising option for the treatment of large cartilage defects. However, because the cell viability of osteochondral tissues (OCTs) gradually reduces during storage at 4°C, methods for maintaining the cell viability of fresh OCTs are needed to improve transplantation outcomes. Here, we evaluated whether the supplementation of preservation solution with one of three different molecular weight forms of hyaluronic acid (HA) improved the viability of rat OCTs during long-term cold storage. The supplementation of University of Wisconsin (UW) solution with 800 kDa significantly improved the cell viability of OCT after 14 days at 4°C compared to nonsupplemented UW solution. In contrast, UW solution supplemented with either 1900 or 6000 kDa HA did not markedly improve the cell viability of the OCT. Real-time PCR analysis revealed that the levels of matrix metalloproteinases 2, 3, and 9 were significantly decreased in OCT stored in UW solution supplemented with 800 kDa HA. Although further studies in human OCT are warranted, these findings demonstrate that the use of 800 kDa HA in place of serum may be a suitable approach for the long-term preservation of osteochondral allografts designated for the repair of large cartilage defects in the clinical setting.

Towards a Clinically Applicable Tissue Engineered Vascular Graft

2013 ◽  
Author(s):  
W. S. Sheridan ◽  
O. Grant ◽  
A. Lopez-Noriega ◽  
G. P. Duffy ◽  
B. P. Murphy
Keyword(s):  
Shelf Life ◽  
Vascular Graft ◽  
Vascular Tissue ◽  
Limiting Factors ◽  
Tissue Replacement ◽  
Large Patient ◽  
Arterial Tissue ◽  
Long Term Preservation

Decellularized arterial tissue has shown promising use as a scaffold for vascular tissue replacement; similar structural and functional characteristics to the native tissue are maintained and these scaffolds are non-thrombogenic, non immunogenic with the ability to remodel and grow in vivo[1]. However, there still remains a number of limiting factors in clinically translating these scaffolds. Namely, producing a range of geometries to accommodate a large patient cohort within clinically feasible manufacturing times and costs. Furthermore, these scaffolds must be suitable for long term preservation to produce a reasonable shelf life and be capable of undergoing standard sterilization techniques.

[18F]Fluoride PET Indicates Reduced Bone Formation in Severe Glucocorticoid-induced Osteoporosis

1998 ◽  
Vol 37 (02) ◽  
pp. 76-79 ◽  
Author(s):  
T. D. Kirchhoff ◽  
W. Burchert ◽  
J. v. d. Hoff ◽  
H. Zeidler ◽  
H. Hundeshagen ◽  
...  
Keyword(s):  
Bone Formation ◽  
Multiple Organ ◽  
High Dose ◽  
Multiple Fractures ◽  
Glucocorticoid Treatment ◽  
Organ Manifestations ◽  
Diagnostic Benefit ◽  
Sharp Syndrome ◽  
18F Fluoride

SummaryA 61-year-old female patient presenting with mixed connective tissue disease (Sharp syndrome), underwent a long-term high dose glucocorticoid treatment because of multiple organ manifestations. Under steroid therapy she developed severe osteoporosis resulting in multiple fractures. A dynamic [18F]fluoride PET study in this patient revealed reduced fluoride influx in non-fractured vertebrae. This finding corresponds to pathogenetic concepts which propose an inhibition of bone formation as major cause of glucocorticoid-induced osteoporosis. In the light of the presented case it seems to be promising to evaluate the diagnostic benefit of [18F]fluoride PET in osteoporosis.

Problems of treatment of primary openangle glaucoma in combination with thrombosis of the central retinal vein

GlaucomaNews ◽  
2020 ◽  
pp. 65-69
Author(s):  
T.E. Lipatkina ◽  
◽  
Е.V. Karlova ◽  
A.V. Zolotarev ◽  
◽  
...  
Keyword(s):  
Macular Edema ◽  
Primary Open Angle Glaucoma ◽  
Antihypertensive Drugs ◽  
Open Angle Glaucoma ◽  
Vascular Wall ◽  
Open Angle ◽  
Retinal Edema ◽  
Long Term Preservation ◽  
Central Retinal Vein

Patients with primary open-angle glaucoma (POAG) and ophthalmic hypertension have an increased likelihood of developing occlusions (thrombosis) of the central retinal vein. Different groups of antihypertensive drugs differ in their mechanism of action and may affect concomitant ocular pathology, in particular, retinal edema, which occurs, for example, in occlusion of the central retinal vein. Used in most patients with glaucoma, prostaglandin analogs can contribute to the long-term preservation of macular edema due to the effect on the permeability of the vascular wall. Preparations of other pharmacological groups, reducing the production of aqueous humor, on the contrary, may contribute to its regression. Therefore, the question of choosing a drug for antihypertensive therapy in patients with primary open-angle glaucoma and concomitant macular edema is relevant and is for further study.

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