Tumor Markers in Squamous Cell Carcinoma of Esophagus: Immunometric Assay in Cytosol and Membrane Fraction

Carcinoembryonic antigen (CEA), tissue polypeptide antigen (TPA), ferritin, and the monoclonal antibody-detected tumor-associated antigens CA19.9 and CA50 were measured by radioimmunoassay in tissue fractions of carcinoma and normal esophageal mucosa from 59 patients with untreated primary squamous cell carcinoma of the esophagus. Tumor markers were measured in cytosol (118 samples) and in a membrane-enriched fraction (32 samples). CEA, TPA and ferritin were detected in almost all the cytosol samples evaluated, CA19.9 and CA50 in 66% and 50% of cases respectively. Ferritin was significantly higher in carcinoma than in normal mucosa. The cytosol concentrations of CEA, TPA, CA19.9 and CA50 were not significantly different in carcinoma and normal tissue. Concentrations of CEA, CA19.9 and CA50 in the membrane fraction tended to be higher in normal tissue than in carcinoma, whereas the cytosol-to-membrane ratio was significantly higher in carcinoma. For CEA, CA19.9 and CA50, the phenotypic pattern of the malignant transformation seems to involve a different intracellular distribution rather than a quantitative change. No correlations were found between tissue and serum concentrations of the tumor markers, the former being related to the phenotypic characteristics of the tumor, the latter to the tumor burden.

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Increasing stepwise expression of p16 e pRb proteins in esophageal mucosa from patients at high risk for squamous cell carcinoma.

Journal of Clinical Oncology ◽

10.1200/jco.2013.31.15_suppl.e15067 ◽

2013 ◽

Vol 31 (15_suppl) ◽

pp. e15067-e15067

Author(s):

Leandro Bizarro Muller ◽

Luis Carlos Moreira Antunes ◽

Antonio de Barros Lopes ◽

Sara Vanazzi ◽

Luise Meurer ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Carcinoma In Situ ◽

Normal Mucosa ◽

Lesion Group ◽

Esophageal Mucosa ◽

Carcinoma Of The Esophagus ◽

Group 1

e15067 Background: Squamous cell carcinoma of the esophagus is a lethal cancer which carries a poor prognosis due to late diagnosis. Identification of molecular markers may aid early diagnosis. We assessed the expression of p16 and pRb in esophageal mucosa and their associations with histology. Methods: Esophageal biopsies were collected from 38 patients with no esophageal lesion (group 1); from iodine-negative and normally staining areas from 108 alcoholic smokers (group 2); and from tumor and non-tumor areas from 41 patients with squamous cell carcinoma (group 3). The histological diagnoses were compared with immunoexpression of p16 and pRb (defined as positive staining in >10% of the cells). Results: In group 1, the biopsies showed only normal mucosa or mild esophagitis, and no expression of p16 or pRb. In groups 2 and 3 the diagnoses were: normal mucosa (38.4%); esophagitis (44.4%); dysplasia or carcinoma in situ (2.8%) and carcinoma (14.3%). The percentage of cases with immunoexpression of p16 and pRb increased in a stepwise fashion over the histologic spectrum from normal mucosa to esophagitis, dysplasia/carcinoma in situ and carcinoma (p for trend < 0.01). Conclusions: Esophageal mucosa exposed to alcohol and tobacco which expresses these proteins may be at risk for malignant transformation.

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PS02.048: DECREASED EXPRESSION OF PRSS27 IN ESOPHAGEAL SQUAMOUS CELL CARCINOMA

Diseases of the Esophagus ◽

10.1093/dote/doy089.ps02.048 ◽

2018 ◽

Vol 31 (Supplement_1) ◽

pp. 134-134

Author(s):

Masayoshi Terayama ◽

Teruki Hagiwara ◽

Kazuhiko Yamada ◽

Daisuke Soma ◽

Kyoko Nohara ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Clinical Features ◽

Squamous Cell ◽

Cell Layer ◽

Normal Mucosa ◽

Esophageal Mucosa ◽

Gene And Protein Expression ◽

Significant Difference

Abstract Background Alcohol drinking and smoking are substantial risk factors of esophageal squamous cell carcinoma (ESCC) and are supposed to induce genetic mutations and epigenetic disorders, including aberrant DNA methylation. Previously, we have conducted transcriptome and methylome analyses of a paired specimen of ESCC and adjacent non-cancerous tissues and found that both gene expression and promotor methylation of PRSS27 were perturbed in ESCC. PRSS27 was a trypsin-like serine protease (also known as marapsin) and expressed in normal esophagus; however, little is known about the significance of PRSS27 expression in ESCC. In this study, we evaluated the expression of PRSS27 in many ESCC cases in relation with clinical features and the prognosis. Methods ESCC tissue specimens were obtained from 80 patients who had undergone esophagectomy between April 2008 and December 2016 in our hospital and were subjected to immunostaining for PRSS27. ESCC cases were classified into PRSS27-negative and PRSS27-positive groups and difference of clinical features and the prognosis between the groups was analyzed. Results The mRNA expression of PRSS27 was significantly decreased in ESCC compared with those in matched normal mucosa (P < 0.0001). Histologically, PRSS27 was highly expressed in spinous cells of suprabasal cell layer but not in basal cell layer of normal esophageal mucosa. In contrast, 37 of 80 (47%) ESCC exhibited decreased intensity of PRSS27 staining when compared with that in normal mucosa, and 53% (43/80) of ESCC showed almost no staining of PRSS27. Although the prognosis of PRSS27-positive cases were worse in trend than that of PRSS27-negative cases, there was no significant difference (P = 0.0763) Conclusion PRSS27 gene and protein expression was both downregulated in ESCC; its functional significance in relation to malignancy is underinvestigation. Disclosure All authors have declared no conflicts of interest.

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Tumor Markers in Serum of Patients with Primary Squamous Cell Carcinoma of the Esophagus

Tumori Journal ◽

10.1177/030089168907500519 ◽

1989 ◽

Vol 75 (5) ◽

pp. 489-493 ◽

Cited By ~ 9

Author(s):

Massimo Gion ◽

Carlo Tremolada ◽

Riccardo Mione ◽

Paolo della Palma ◽

Ruggero Dittadi ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Cancer ◽

Cell Carcinoma ◽

Tumor Markers ◽

Squamous Cell ◽

Clinical Stage ◽

Stage Iv ◽

Blood Levels ◽

Primary Squamous Cell Carcinoma ◽

Carcinoma Of The Esophagus

Serum levels of several tumor markers were studied in 96 patients with untreated primary squamous cell carcinoma of the esophagus. Three markers specific for digestive tract malignancies - CEA, CA19.9 and CA50 - and two non organ specific indicators of malignancy - ferritin and TPA - were evaluated. Positivity rates of CAI9.9 and CA50 were very low (4.4 % and 8.6 % respectively); the markers were therefore considered ineffective in the disease. CEA, TPA and ferritin showed a fair positivity rate (27.1 %, 28.1 %, 33.7% respectively); CEA and TPA were directly related to clinical stage, CEA levels being significantly higher in stage IV than in stage III cases (p = 0.016). TPA preoperatory levels were also directly related to a lower survival probability (p = 0.004). CEA showed significantly lower levels in tumors of lower than in those of middle (p = 0.03) and upper esophagus (p = 0.004). TPA showed a similar behaviour with lower levels in tumors of lower than of middle esophagus (p = 0.03). These findings could be due to a bulky metabolism of tumor markers drained via portail vein in the liver. From our data the following conclusions may be drawn: 1) CEA and TPA may be useful in the staging of esophageal cancer as an ancillary tool to assess the extent of the disease; 2) tumor location is an important variable when evaluating blood levels of tumor markers in patients with esophageal cancer.

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P53 protein in esophageal mucosa of individuals at high-risk for squamous cell carcinoma of the esophagus

Gastroenterology ◽

10.1016/s0016-5085(00)82212-x ◽

2000 ◽

Vol 118 (4) ◽

pp. A36

Author(s):

Renato B. Fagundes ◽

Sergio Gs Barros ◽

Carlos R. Melo ◽

Mario B. Wagner ◽

Patricia Tollens ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

High Risk ◽

Cell Carcinoma ◽

Squamous Cell ◽

P53 Protein ◽

Esophageal Mucosa ◽

Carcinoma Of The Esophagus

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Comparison of COX2 Expression between Oral Squamous Cell Carcinoma, Leukoplakia and Normal Mucosa

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1122 ◽

2012 ◽

Vol 13 (2) ◽

pp. 205-209 ◽

Cited By ~ 3

Author(s):

Maryam Amirchaghmaghi ◽

Nooshin Mohtasham ◽

Pegah Mosannen Mozaffari

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Normal Tissue ◽

Basal Layer ◽

Normal Mucosa ◽

Significant Difference ◽

Grade 3 ◽

Cox2 Expression

ABSTRACT Aim To compare cyclooxygenase 2 expression (COX2-E) between normal, oral leukoplakia lesions and different grades of oral squamous cell carcinoma (SCC). Materials and methods Around 90 paraffin embedded blocks consisting of 45 SCC, 15 leukoplakia and 17 controls were selected for immunohistochemistry (IHC) for detection of COX2- E. COX2-E was divided in four grades, as A (0-10%), B (11-40%), C (41-70%) and D (> 70%) cellularity. Results Mean age of the patients was 55.17 ± 18.41 (M:57.92 ± 16.87, F:52.19 ± 19.74). A significant difference was found in COX2 expression between SCC total and, basal and spinous layers of leukoplakia (p < 0.05). COX2-E in spinous layer of normal tissue was significantly lower than SCC (p = 0.000). COX2-E was significantly different in SCC grade 3 and leukoplakia (p = 0.001) and normal tissue (p = 0.000). COX2-E was significantly higher in SCC grade 3 compared to leukoplakia (basal layer) (p = 0.000). Conclusion We showed a significant higher COX2-E in SCC lesions compared to leukoplakias and normal controls. In our study COX2-E was not significantly different in SCC grades 1, 2 and 3 (p > 0.05). How to cite this article Amirchaghmaghi M, Mohtasham N, Mozaffari PM. Comparison of COX2 Expression between Oral Squamous Cell Carcinoma, Leukoplakia and Normal Mucosa. J Contemp Dent Pract 2012;13(2):205-209.

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