scholarly journals Quantification and Comparison of Extraction Methods for Alkaloids in Aegle marmelos Leaves by HPLC

2014 ◽  
Vol 9 (7) ◽  
pp. 1934578X1400900
Author(s):  
Aniket Karmase ◽  
K Prasanna ◽  
Sruti Rasabattula ◽  
Kamlesh K Bhutani
Keyword(s):  
Hplc Analysis ◽  
Extraction Methods ◽  
Hplc Method ◽  
Good Recovery ◽  
Aegle Marmelos ◽  
Low Concentrations ◽  
Validation Parameters ◽  
Marker Compounds ◽  
Quantitative Hplc Analysis ◽  
Quantitative Hplc

The leaves of Aegle marmelos are reported to contain multi-bioactive classes of compounds including coumarins, furanocoumarins and alkaloids. HPLC analysis of the crude extract was challenging due to low concentrations of the compounds in the leaves. Five compounds visible in the HPLC chromatogram were separated and identified by HPLC and further elaborated for quantification as marker compounds of A. marmelos leaves using a C18 column with detection at 275 nm. A gradient mobile phase consisting of acetonitrile and water was used. The developed HPLC method showed good linearity (r2≥0.994), high precision (RSD<5%), and good recovery (99.27–99.98%) of the compounds. The lowest detection limit was 5 ng and the method was found to be robust. All the validation parameters were within the permissible limits. Therefore, the developed method is accurate and reliable for the quality control of A. marmelos. This is the first report of extensive quantitative HPLC analysis of marker compounds in A. marmelos leaves and method validation.

scholarly journals Analysis of Homoisoflavonoids in Caesalpinia digyna by HPLC-ESI-MS, HPLC and Method Validation

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Somendu K. Roy ◽  
Amit Srivastava ◽  
Sanjay M. Jachak
Keyword(s):  
Method Validation ◽  
Mobile Phase ◽  
Hplc Method ◽  
Good Recovery ◽  
Esi Ms ◽  
Validation Parameters ◽  
Quantitative Hplc Analysis ◽  
Quantitative Hplc ◽  
Hplc Analyses

The roots of Caesalpinia digyna have been reported to contain gallic acid derivatives and minor homoisoflavonoids, but HPLC-ESI-MS and HPLC analyses of the homoisoflavonoids were challenging due to their low concentration in the roots. Separation and identification was accomplished by HPLC-ESI-MS and further elaborated for quantification using a C18 column with detection at 330 nm. A gradient mobile phase consisting of methanol and water (0.1% acetic acid) was used. The developed HPLC method showed good linearity (r2≥0.998), high precision (RSD<5%) and a good recovery (99.3-104.5%) of the compounds. The lowest detection limit was 0.75 ng and the method was found to be robust. All the validation parameters were found to be within the permissible limits and, therefore, the developed method is accurate and reliable for the quality control of C. digyna and other Caesalpinia species. This is the first report of sample preparation on Diaion HP-20 resin and characterization of homoisoflavonoids by HPLC-ESI-MS, extended by extensive quantitative HPLC analysis of homoisoflavonoids in C. digyna roots and method validation.

scholarly journals Phtytochemical Studies and Quantitative HPLC Analysis of Rosmarinic Acid and Luteolin 5-O-β-D-Glucopyranoside on Thymus praecox subsp. grossheimii var. grossheimii

2015 ◽  
Vol 63 (9) ◽  
pp. 720-725 ◽  
Author(s):  
Handan Gokben Sevindik ◽  
Ufuk Ozgen ◽  
Alptug Atila ◽  
Handan Ozturk Er ◽  
Cavit Kazaz ◽  
...  
Keyword(s):  
Rosmarinic Acid ◽  
Hplc Analysis ◽  
Quantitative Hplc Analysis ◽  
Quantitative Hplc

scholarly journals Quantitative HPLC Analysis of Catechin in Wound- Associated Wood and Knots of Beech

2013 ◽  
Vol 64 (3) ◽  
pp. 231-238 ◽  
Author(s):  
Viljem Vek ◽  
Primož Oven ◽  
Ida Poljanšek
Keyword(s):  
Hplc Analysis ◽  
Quantitative Hplc Analysis ◽  
Quantitative Hplc

scholarly journals Identification of Cardioactive Leonurus and Leonotis Drugs by Quantitative HPLC Determination and HPTLC Detection of Phenolic Marker Constituents

2016 ◽  
Vol 11 (8) ◽  
pp. 1934578X1601100
Author(s):  
Kenny Kuchta ◽  
Jutta Ortwein ◽  
İhsan Çaliş ◽  
Rainer B. Volk ◽  
Hans W. Rauwald
Keyword(s):  
Quality Control ◽  
Ferulic Acid ◽  
South African ◽  
Neurological Disorders ◽  
Hplc Analysis ◽  
Hplc Method ◽  
Rp Hplc ◽  
Fruit Samples ◽  
Leonurus Cardiaca ◽  
Quantitative Hplc

Officinal European Leonurus cardiaca, East Asian L. japonicus, and South African Leonotis leonurus are traditionally used for cardiovascular, gynecological, and neurological disorders. Nevertheless, a phytochemical assessment as a basis for their quality control and comparison amongst them has not yet been reported up to now. Here, a novel RP-HPLC method is presented for quantifying twelve phenolics, lavandulifolioside, verbascoside, hyperoside, ferulic acid, isoquercitrin, rutoside, apigenin-7- O-D-glucoside, and quercitrin, as well as chlorogenic, caffeic, rosmarinic, and cichoric acids, in 18 herbal and fruit samples of the three species, as well as in a L. cardiaca refined extract. Only ferulic acid was found in every sample, whereas rosmarinic acid and apigenin-7- O-D-glucoside were not detected in any sample. Chlorogenic, caffeic, and cichoric acids and rutoside were detected in all three species. Lavandulifolioside and verbascoside, the dominant phenolics of L. cardiac, were not present in any sample of L. japonicus. Lavandulifolioside was found in this first ever HPLC analysis on phenolics of L. leonurus. Hyperoside was not found in L. cardiaca, but in both L. japonicus and L. leonurus, whereas isoquercitrin was detected in L. cardiaca and L. leonurus, but not in L. japonicus. This approach facilitates identification and quality control via HPLC/HPTLC fingerprints.

Method of quantitative HPLC analysis in investigation of cyclocondensation of 2-pyrrolidone with anthranilic acid

1989 ◽  
Vol 25 (2) ◽  
pp. 258-259
Author(s):  
Kh. R. Nuriddinov ◽  
K. Sargazakov ◽  
Sh. Abdullaev ◽  
Kh. N. Aripov
Keyword(s):  
Anthranilic Acid ◽  
Hplc Analysis ◽  
Quantitative Hplc Analysis ◽  
Quantitative Hplc
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