scholarly journals Pterostilbene and Its Glucoside Induce Type XVII Collagen Expression

2017 ◽  
Vol 12 (1) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Hiroki Hamada ◽  
Kei Shimoda ◽  
Yoshiyuki Horio ◽  
Tsubasa Ono ◽  
Ryusuke Hosoda ◽  
...  
Keyword(s):  
Human Skin ◽  
Cell Model ◽  
Plant Cells ◽  
Phytolacca Americana ◽  
Collagen Expression ◽  
Skin Cell ◽  
Xvii Collagen

The glycosylation of pterostilbene by cultured plant cells of Phytolacca americana gave pterostilbene 4′- O-β-D-glucoside. Both pterostilbene and its 4- O-β-D-glucoside induced type XVII collagen expression in the EpiDermFT EFT-400 human skin cell model. Pterostilbene 4′- O-β-D-glucoside strongly induced type XVII collagen expression rather than pterostilbene.

Does Shape Matter? Bioeffects of Gold Nanomaterials in a Human Skin Cell Model

Langmuir ◽  
2012 ◽  
Vol 28 (6) ◽  
pp. 3248-3258 ◽  
Author(s):  
Nicole M. Schaeublin ◽  
Laura K. Braydich-Stolle ◽  
Elizabeth I. Maurer ◽  
Kyoungweon Park ◽  
Robert I. MacCuspie ◽  
...  
Keyword(s):  
Human Skin ◽  
Cell Model ◽  
Skin Cell ◽  
Gold Nanomaterials

scholarly journals Personalized Development of Antisense Oligonucleotides for Exon Skipping Restores Type XVII Collagen Expression in Junctional Epidermolysis Bullosa

2021 ◽  
Vol 22 (7) ◽  
pp. 3326
Author(s):  
Michael Ablinger ◽  
Thomas Lettner ◽  
Nicole Friedl ◽  
Hannah Potocki ◽  
Theresa Palmetzhofer ◽  
...  
Keyword(s):  
Epidermolysis Bullosa ◽  
Antisense Oligonucleotides ◽  
Exon Skipping ◽  
Junctional Epidermolysis Bullosa ◽  
Reading Frame ◽  
Collagen Expression ◽  
Promising Tool ◽  
Mucous Membranes ◽  
Personalized Approach ◽  
Xvii Collagen

Intermediate junctional epidermolysis bullosa caused by mutations in the COL17A1 gene is characterized by the frequent development of blisters and erosions on the skin and mucous membranes. The rarity of the disease and the heterogeneity of the underlying mutations renders therapy developments challenging. However, the high number of short in-frame exons facilitates the use of antisense oligonucleotides (AON) to restore collagen 17 (C17) expression by inducing exon skipping. In a personalized approach, we designed and tested three AONs in combination with a cationic liposomal carrier for their ability to induce skipping of COL17A1 exon 7 in 2D culture and in 3D skin equivalents. We show that AON-induced exon skipping excludes the targeted exon from pre-mRNA processing, which restores the reading frame, leading to the expression of a slightly truncated protein. Furthermore, the expression and correct deposition of C17 at the dermal–epidermal junction indicates its functionality. Thus, we assume AON-mediated exon skipping to be a promising tool for the treatment of junctional epidermolysis bullosa, particularly applicable in a personalized manner for rare genotypes.

scholarly journals Effect of Natural Plant Water Extracts Suppressed Melanin Production In Human skin cell(CCD‐986sk)

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Jung Yeong Jang ◽  
Mi Ja Chung ◽  
Jin kyoun You ◽  
Dae Jung Kim ◽  
Dong Joo Seo ◽  
...  
Keyword(s):  
Human Skin ◽  
Plant Water ◽  
Melanin Production ◽  
Natural Plant ◽  
Water Extracts ◽  
Skin Cell

scholarly journals Glycosylation and Methylation of Quercetin and Myricetin by Cultured Cells of Phytolacca americana

2017 ◽  
Vol 12 (4) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Yuya Fujitaka ◽  
Kei Shimoda ◽  
Naoji Kubota ◽  
Minami Araki ◽  
Tatsuya Onishi ◽  
...  
Keyword(s):  
Cultured Cells ◽  
Plant Cells ◽  
Phytolacca Americana

The glycosylation and methylation of quercetin by cultured plant cells of Phytolacca americana gave quercetin 3- O-β-D-glucoside and isorhamnetin 3- O-β-D-glucoside. Myricetin was glycosylated and methylated to syringetin 3- O-β-D-glucoside by cultured P. americana cells.

Human skin cell cultures onto PLA50(PDLLA) bioresorbable polymers: Influence of chemical and morphological surface modifications

2004 ◽  
Vol 72A (2) ◽  
pp. 180-189 ◽  
Author(s):  
Xavier Garric ◽  
Jean-Pierre Molès ◽  
Henri Garreau ◽  
Jean-Jacques Guilhou ◽  
Michel Vert
Keyword(s):  
Human Skin ◽  
Cell Cultures ◽  
Surface Modifications ◽  
Bioresorbable Polymers ◽  
Skin Cell

Quantitative analysis of MC1R gene expression in human skin cell cultures

2006 ◽  
Vol 19 (1) ◽  
pp. 76-89 ◽  
Author(s):  
Donald W. Roberts ◽  
Richard A. Newton ◽  
Kimberley A. Beaumont ◽  
J. Helen Leonard ◽  
Richard A. Sturm
Keyword(s):  
Gene Expression ◽  
Quantitative Analysis ◽  
Human Skin ◽  
Cell Cultures ◽  
Mc1r Gene ◽  
Skin Cell

scholarly journals Soapwort (Saponaria officinalis L.) Extract vs. Synthetic Surfactants—Effect on Skin-Mimetic Models

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5628
Author(s):  
Ilona Jurek ◽  
Aleksandra Szuplewska ◽  
Michał Chudy ◽  
Kamil Wojciechowski
Keyword(s):  
Human Skin ◽  
Melanoma Cells ◽  
Engine Oil ◽  
Molar Ratio ◽  
Lauryl Sulfate ◽  
Protein Solubilization ◽  
Skin Cell ◽  
Emulsification Capacity ◽  
Cocamidopropyl Betaine ◽  
Synthetic Surfactants

Our skin is continuously exposed to different amphiphilic substances capable of interaction with its lipids and proteins. We describe the effect of a saponin-rich soapwort extract and of four commonly employed synthetic surfactants: sodium lauryl sulfate (SLS), sodium laureth sulfate (SLES), ammonium lauryl sulfate (ALS), cocamidopropyl betaine (CAPB) on different human skin models. Two human skin cell lines were employed: normal keratinocytes (HaCaT) and human melanoma cells (A375). The liposomes consisting of a dipalmitoylphosphatidylcholine/cholesterol mixture in a molar ratio of 7:3, mimicking the cell membrane of keratinocytes and melanoma cells were employed as the second model. Using dynamic light scattering (DLS), the particle size distribution of liposomes was analyzed before and after contact with the tested (bio)surfactants. The results, supplemented by the protein solubilization tests (albumin denaturation test, zein test) and oil emulsification capacity (using olive oil and engine oil), showed that the soapwort extract affects the skin models to a clearly different extent than any of the tested synthetic surfactants. Its protein and lipid solubilizing potential are much smaller than for the three anionic surfactants (SLS, ALS, SLES). In terms of protein solubilization potential, the soapwort extract is comparable to CAPB, which, however, is much harsher to lipids.

scholarly journals Ferulic Acid, Methyl Ferulate, and Ferulic Acid Glucopyranosyl Ester Isolated from Cultured Cells of Phytolacca americana

2018 ◽  
Vol 13 (1) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Kei Shimoda ◽  
Naoji Kubota ◽  
Hiroki Hamada ◽  
Shouta Doi ◽  
Kohji Ishihara ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Ferulic Acid ◽  
Cultured Cells ◽  
Plant Cells ◽  
Phytolacca Americana ◽  
Acid Methyl ◽  
Methyl Ferulate

Production of secondary metabolites in cultured plant cells of Phytolacca americana was investigated. Three compounds, i.e., ferulic acid, methyl ferulate, and ferulic acid glucopyranosyl ester, were isolated from cultured P. americana cells.

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