scholarly journals Fungus-Growing Ant's Microbial Interaction of Streptomyces sp. and Escovopsis sp. through Molecular Networking and MALDI Imaging

2019 ◽  
Vol 14 (1) ◽  
pp. 1934578X1901400 ◽  
Author(s):  
Cristopher A. Boya P. ◽  
Martin H. Christian ◽  
Hermógenes Fernández-Marín ◽  
Marcelino Gutiérrez
Keyword(s):  
Mass Spectrometry ◽  
Microbial Interactions ◽  
Maldi Imaging ◽  
Microbial Interaction ◽  
Molecular Networking ◽  
Streptomyces Sp ◽  
Desferrioxamine B ◽  
Ferrioxamine B ◽  
Fungus Growing Ants ◽  
Acromyrmex Echinatior

Microbes associated with fungus-growing ants represent a poorly explored source of natural products. In this study, we used mass spectrometry-based dereplication techniques for identifying a set of secondary metabolites produced during the microbial interaction between Streptomyces sp. (CB0028) and Escovopsis sp. (CBAcro424). Both microorganisms were isolated from the nest of the fungus-growing ant Acromyrmex echinatior. Through MALDI imaging and MS/MS molecular networking, we annotated the siderophores: desferrioxamine B (1), ferrioxamine B (2), ferrioxamine E (3) and the N-formylated peptide SCO-2138/SLI-2138 (4). MALDI imaging experiments suggest that siderophores occurred during the microbial interactions in the fungus-growing ants – microbes symbioses. This is the first report on the production of compounds 1-4 by bacteria associated with fungus-growing ants.

MALDI imaging mass spectrometry: an emerging tool in neurology

2021 ◽  
Author(s):  
Laura K. Schnackenberg ◽  
David A. Thorn ◽  
Dustyn Barnette ◽  
E. Ellen Jones
Keyword(s):  
Mass Spectrometry ◽  
Imaging Mass Spectrometry ◽  
Maldi Imaging ◽  
Maldi Imaging Mass Spectrometry

scholarly journals The Tripod for Bacterial Natural Product Discovery: Genome Mining, Silent Pathway Induction, and Mass Spectrometry-Based Molecular Networking

mSystems ◽  
2018 ◽  
Vol 3 (2) ◽  
Author(s):  
Daniela B. B. Trivella ◽  
Rafael de Felicio
Keyword(s):  
Mass Spectrometry ◽  
Natural Products ◽  
Natural Product ◽  
Biosynthetic Pathway ◽  
Genome Mining ◽  
Chemical Compounds ◽  
Gene Clusters ◽  
Tandem Mass ◽  
Molecular Networking ◽  
Natural Product Discovery

ABSTRACT Natural products are the richest source of chemical compounds for drug discovery. Particularly, bacterial secondary metabolites are in the spotlight due to advances in genome sequencing and mining, as well as for the potential of biosynthetic pathway manipulation to awake silent (cryptic) gene clusters under laboratory cultivation. Further progress in compound detection, such as the development of the tandem mass spectrometry (MS/MS) molecular networking approach, has contributed to the discovery of novel bacterial natural products. The latter can be applied directly to bacterial crude extracts for identifying and dereplicating known compounds, therefore assisting the prioritization of extracts containing novel natural products, for example. In our opinion, these three approaches—genome mining, silent pathway induction, and MS-based molecular networking—compose the tripod for modern bacterial natural product discovery and will be discussed in this perspective.

scholarly journals Region-specific bioconversion of dynorphin neuropeptide detected by in situ histochemistry and MALDI imaging mass spectrometry

Peptides ◽  
2017 ◽  
Vol 87 ◽  
pp. 20-27 ◽  
Author(s):  
Erik Bivehed ◽  
Robert Strömvall ◽  
Jonas Bergquist ◽  
Georgy Bakalkin ◽  
Malin Andersson
Keyword(s):  
Mass Spectrometry ◽  
Imaging Mass Spectrometry ◽  
Maldi Imaging ◽  
Maldi Imaging Mass Spectrometry

Modelling competition between the siderophore ferrioxamine B and humic like binding sites in seawater v1

2021 ◽  
Author(s):  
Kechen Zhu ◽  
Martha Gledhill
Keyword(s):  
Binding Sites ◽  
Major Ions ◽  
Side Reaction ◽  
Iron Solubility ◽  
Desferrioxamine B ◽  
Thermodynamic Constants ◽  
Reaction Coefficient ◽  
Ferrioxamine B ◽  
Donnan Model

Here we archive a protocol that can be used to determine competition between a siderophore (ferrioxamine B) and humic like binding sites that are present in marine DOM. We use the NICA-Donnan model to describe binding by humic like binding sites in DOM. Constants for Fe binding to marine DOM are taken from Zhu et al., (2021). Thermodynamic constants describing binding between major ions, iron and ferrioxamine B are taken from Schijf and Burns, (2016). References Schijf, J., Burns, S.M., 2016. Determination of the Side-Reaction Coefficient of Desferrioxamine B in Trace-Metal-Free Seawater. Front. Mar. Sci. 3, 117. https://doi.org/10.3389/fmars.2016.00117 Zhu, K., Birchill, A.J., Milne, A., Ussher, S.J., Humphreys, M.P., Carr, N., Mahaffey, C., Lohan, M.C., Achterberg, E.P., Gledhill, M., 2021a. Equilbrium calculations of iron speciation and apparent iron solubility in the Celtic Sea at ambient pH using the NICA-Donnan model. Mar. Chem

scholarly journals Ultraviolet (IUV) and mass spectrometry (IMS) imaging for the deconvolution of microbial interactions

2018 ◽  
Vol 12 (S5) ◽  
Author(s):  
Víctor González-Menéndez ◽  
Germán Martínez ◽  
Rachel Serrano ◽  
Francisca Muñoz ◽  
Jesús Martín ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Microbial Interactions

MALDI Imaging Mass Spectrometry – A Novel Approach in Biomedical Research of Tissues

2013 ◽  
Vol 10 (2) ◽  
pp. 76-82
Author(s):  
Magdalena Kalinowska-Herok ◽  
Monika Pietrowska ◽  
Anna Walaszczyk ◽  
Piotr Widak
Keyword(s):  
Mass Spectrometry ◽  
Biomedical Research ◽  
Imaging Mass Spectrometry ◽  
Maldi Imaging ◽  
Novel Approach ◽  
Maldi Imaging Mass Spectrometry
Sign in / Sign up

Export Citation Format

Share Document