scholarly journals A Simple Analysis Method for 4-Deoxy-l-erythro-5-hexoseulose Uronic Acid by HPLC-ELSD with Column for Anion Analysis

2019 ◽  
Vol 14 (5) ◽  
pp. 1934578X1985099
Author(s):  
Toshiyuki Shibata ◽  
Reona Fujii ◽  
Yoshihiko Nishioka ◽  
Hideo Miyake ◽  
Tetsushi Mori ◽  
...  
Keyword(s):  
High Performance ◽  
Uronic Acid ◽  
Alginate Lyase ◽  
Signal Noise ◽  
Light Scattering Detection ◽  
Evaporative Light Scattering ◽  
Deoxy Sugar ◽  
Lyase Activity ◽  
Noise Ratio ◽  
Unsaturated Disaccharide

4-Deoxy-l- erythro-5-hexoseulose uronic acid (DEH) is a rare deoxy sugar produced from alginate by the action of an exotype alginate lyase. A simple and rapid method for analyzing DEH using high-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) was developed in this study. For chromatography, an isocratic elution of ammonium formate buffer including formic acid and a column for anion chromatography were used. In the developed method, DEH was detected at a retention time of 3.038 minutes and limits of detection (signal-noise ratio = 3) and quantification (signal-noise ratio = 10) were 37.5 and 124.9 µg/mL as a sodium DEH, respectively. In addition, separation and detection of alginate unsaturated oligosaccharides were also tested using the method. Within an analysis time of 10 minutes, it was possible to separate and detect unsaturated disaccharide, unsaturated trisaccharide, and unsaturated tetrasaccharide prepared using poly(β-d-mannuronate) lyase and sodium alginate of high mannuronate type. The HPLC-ELSD method established in this study will be applicable for quantitative analysis of DEH and measurement of exotype alginate lyase activity.

scholarly journals Determination of Triterpenoids and Phenolic Acids from Sanguisorba officinalis L. by HPLC-ELSD and Its Application

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4505
Author(s):  
Jiahui Sun ◽  
Chunli Gan ◽  
Jing Huang ◽  
Zhenyue Wang ◽  
Chengcui Wu ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
High Performance ◽  
Gradient Elution ◽  
Light Scattering Detection ◽  
Evaporative Light Scattering ◽  
Acetic Acid Water ◽  
Sanguisorba Officinalis ◽  
Functional Components ◽  
Gradient Elution Mode

A novel analytical method involving high-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) was developed for simultaneous determination of 11 phenolic acids and 12 triterpenes in Sanguisorba officinalis L. Chromatographic separation was conducted with gradient elution mode by using a DiamonsilTM C18 column (250 mm × 4.6 mm, 5 μm) with the mobile phase of 0.1% acetic acid water (A) and methanol (B). The drift tube temperature of ELSD was set at 70 °C and the nitrogen cumulative flow rate was 1.6 L/min. The method was fully validated to be linear over a wide concentration range (R2 ≥ 0.9991). The precisions (RSD) were less than 3.0% and the recoveries were between 97.7% and 101.4% for all compounds. The results indicated that this method is accurate and effective for the determination of 23 functional components in Sanguisorba officinalis L. and could also be successfully applied to study the influence of processing method on those functional components in Sanguisorba officinalis L.

Determination of mono- and diacylglycerols in edible oils and fats by high performance liquid chromatography and evaporative light scattering detector: Results of collaborative studies and the standardised method

1999 ◽  
Vol 71 (10) ◽  
pp. 1983-1991 ◽  
Author(s):  
D. Berner ◽  
A. Dieffenbacher
Keyword(s):  
Liquid Chromatography ◽  
Light Scattering ◽  
High Performance ◽  
Edible Oils ◽  
Collaborative Study ◽  
Evaporative Light Scattering Detector ◽  
Light Scattering Detection ◽  
Collaborative Studies ◽  
Evaporative Light Scattering

The development, by collaborative study, of standardised method for the determination of mono- and diacylglycerols in vegetable oils and fats is described. The method involves separation of mono- and diacylglycerols by normal phase high-performance liquid-liquid chromatography (HPLC) and evaporative light scattering detection of a solution of oil, fat or a commercial mono- and diacylglycerol preparation in a organic solvent.

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