scholarly journals Assessment of the Biological Effects of Pellia endiviifolia and its Constituents in Vitro

2021 ◽  
Vol 16 (11) ◽  
pp. 1934578X2110564
Author(s):  
Ivana Ivković ◽  
Danka Bukvički ◽  
Miroslav Novaković ◽  
Ivana Majstorović ◽  
Andreja Leskovac ◽  
...  
Keyword(s):  
Anticancer Agents ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Human Peripheral Blood ◽  
Biological Effects ◽  
Human Lymphocytes ◽  
Antioxidant Properties ◽  
Proliferation Index ◽  
Peripheral Blood Cells ◽  
Pellia Endiviifolia

Liverworts are characterized by a high content of bioactive compounds reported to show antimicrobial, anticancer, and antioxidant properties. In this study, the biological effects of the methanol extract of the liverwort Pellia endiviifolia and its constituents, bis-bibenzyls perrottetin E, 10′-hydroxyperrottetin E, and 10,10′-dihydroxyperrottetin E, were investigated using human peripheral blood cells as a model system. The assessment of the investigated compounds comprised testing their genotoxicity, apoptotic potential, and redox modulating activities. The genotoxicity testing indicated that medium (25 µM) and high concentrations (100 µM) of the investigated compounds displayed genotoxic and antiproliferative effects in human lymphocytes as revealed by significant, concentration-dependent enhancement of the micronuclei incidence and decrease in the cytokinesis-block proliferation index compared to the control ( P < .001). Analysis of leukocyte apoptosis showed a substantial potential of all investigated compounds to induce apoptosis, which was not concentration-dependent. The P endiviifolia extract and perrottetin E demonstrated considerable pro-apoptotic potential, even at the lowest concentration (1 µM) applied. Evaluation of the redox modulating effects, which comprised measuring erythrocyte catalase activity and the lymphocyte malondialdehyde level, showed that the investigated compounds did not induce oxidative stress in human peripheral blood cells ( P > .05). The observed genotoxic, antiproliferative, and proapoptotic effects of the investigated compounds make them suitable for further comprehensive studies related to their possible applications as anticancer agents.

scholarly journals Antigenotoxic Properties ofAgaricus blazeiagainst Hydrogen Peroxide in Human Peripheral Blood Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Lada Živković ◽  
Sunčica Borozan ◽  
Andrea Čabarkapa ◽  
Dijana Topalović ◽  
Ummi Ciptasari ◽  
...  
Keyword(s):  
Dna Damage ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Human Peripheral Blood ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
In Vivo Studies ◽  
Peripheral Blood Cells ◽  
Agaricus Blazei ◽  
Mycelial Form

The ability ofAgaricus blazeimushroom in its dried and powdered mycelial form was evaluated for its antigenotoxic properties for the first time. Antigenotoxic effects in human peripheral blood cells against H2O2-induced DNA damage were examined in pretreatment and posttreatment protocol by comet assay. The results showed better antigenotoxic properties ofAgaricus blazeion the interventional level, respectively, after treatment.Agaricus blazeiin concentration of 250 μg/mL after treatment was most efficient in regard to its action against DNA damage. The evaluation of repair kinetics showed decrease in H2O2induced DNA damage 15 min after the application ofA. blazei, reaching the maximum potency after 30 min. Analysis of antioxidant properties ofAgaricus blazeirevealed strongOH•scavenging properties and moderate reducing power, while its DPPH scavenging ability was weak. In regard to our findings, we can conclude that our preliminary results demonstrated antigenotoxic properties ofAgaricus blazeiand its strongOH•scavenging ability. Mechanisms underlying its properties should be further evaluated in in vivo studies.

Gene Expression Analysis in Human Peripheral Blood Cells after 900 MHz RF-EMF Short-Term Exposure

2018 ◽  
Vol 189 (5) ◽  
pp. 529-540 ◽  
Author(s):  
Andreas Lamkowski ◽  
Matthias Kreitlow ◽  
Jörg Radunz ◽  
Martin Willenbockel ◽  
Frank Sabath ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Gene Expression Analysis ◽  
Human Peripheral Blood ◽  
Peripheral Blood Cells ◽  
Short Term ◽  
Short Term Exposure

Genotoxic effects of neurotoxin ß-N-methylamino-l-alanine in human peripheral blood cells

Chemosphere ◽  
2019 ◽  
Vol 214 ◽  
pp. 623-632 ◽  
Author(s):  
Marko Gerić ◽  
Goran Gajski ◽  
Ana-Marija Domijan ◽  
Vera Garaj-Vrhovac ◽  
Metka Filipič ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Blood Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Cells ◽  
Genotoxic Effects

scholarly journals Paracoccidioidomycosis: no genetic damage in human peripheral blood cells of patients assessed by single-cell gel (comet) assay

2007 ◽  
Vol 40 (4) ◽  
pp. 476-478 ◽  
Author(s):  
Renata Aparecida Martinez Antunes Ribeiro-Vieira ◽  
Daniel Araki Ribeiro ◽  
Daisy Maria Favero Salvadori ◽  
Sílvio Alencar Marques
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Human Peripheral Blood ◽  
Paracoccidioides Brasiliensis ◽  
Peripheral Blood Cells ◽  
Genetic Damage ◽  
Systemic Fungal Infection ◽  
Dna Breakage

Paracoccidioidomycosis is a systemic fungal infection caused by Paracoccidioides brasiliensis. As infectious diseases can cause DNA damage, the authors aimed at analyzing DNA breakage in peripheral blood cells of patients with paracoccidioidomycosis by using the comet assay. The results suggested that paracoccidioidomycosis does not cause genotoxicity.

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