scholarly journals Rainbowarray Microsphere-Based Gene Detection Assay

2019 ◽  
Vol 25 (3) ◽  
pp. 258-266
Author(s):  
Ying Xu ◽  
Pei Zhang ◽  
Fan Yi ◽  
Jing Wei ◽  
Quan Du
Keyword(s):  
Target Detection ◽  
High Throughput ◽  
Quantitative Measurement ◽  
Clinical Testing ◽  
Multiplex Detection ◽  
Gene Detection ◽  
Microrna Regulation ◽  
Detection Assay ◽  
Cost Efficient ◽  
High Throughput Manner

Here, we have developed a set of fluorophore-labeled microspheres named rainbowarray microspheres. Based on the spectrally encoded microspheres, we further developed a liquid hybridization approach for multiplex target detection. Different from the prototype Luminex xMAP array, this technology enables feasible, flexible, and cost-efficient microsphere labeling and multiplex detection in a timely and high-throughput manner. To demonstrate the practicability of this technology, quantitative measurement of microRNA regulation was performed during the differentiation of 3T3-L1 cells, in which the expression of two microRNAs was determined at a 2 h interval during a process of 2 days. The flexibility and the timely and high-throughput properties of the technology enable it to be widely implemented in clinical testing.

scholarly journals Multiplexed CRISPR-based microfluidic platform for clinical testing of respiratory viruses and SARS-CoV-2 variants

2021 ◽  
Author(s):  
Nicole L Welch ◽  
Meilin Zhu ◽  
Catherine Hau ◽  
Juliane Weller ◽  
Marzieh Ezzaty Mirhashemi ◽  
...  
Keyword(s):  
High Throughput ◽  
Quantitative Measurement ◽  
Respiratory Virus ◽  
Cost Effective ◽  
Respiratory Viruses ◽  
Variant Classification ◽  
Clinical Use ◽  
Clinical Testing ◽  
Variant Detection ◽  
Grade Performance

The COVID-19 pandemic has demonstrated a clear need for high-throughput, multiplexed, and sensitive assays for detecting SARS-CoV-2 and other respiratory viruses as well as their emerging variants. Here, we present microfluidic CARMEN (mCARMEN), a cost-effective virus and variant detection platform that combines CRISPR-based diagnostics and microfluidics with a streamlined workflow for clinical use. We developed the mCARMEN respiratory virus panel (RVP) and demonstrated its diagnostic-grade performance on 533 patient specimens in an academic setting and then 166 specimens in a clinical setting. We further developed a panel to distinguish 6 SARS-CoV-2 variant lineages, including Delta and Omicron, and evaluated it on 106 patient specimens, with near-perfect concordance to sequencing-based variant classification. Lastly, we implemented a combined Cas13 and Cas12 approach that enables quantitative measurement of viral copies in samples. mCARMEN enables high-throughput surveillance of multiple viruses and variants simultaneously.

Kinetic Solvent Effects in Organic Reactions

2017 ◽  
Author(s):  
Belinda Slakman ◽  
Richard West
Keyword(s):  
Solvent Effect ◽  
Reaction Kinetics ◽  
Computational Methods ◽  
High Throughput ◽  
Kinetic Modeling ◽  
Solvent Effects ◽  
Reaction Rates ◽  
Prior Work ◽  
Solvent Phase ◽  
High Throughput Manner

<div> <div> <div> <p>This article reviews prior work studying reaction kinetics in solution, with the goal of using this information to improve detailed kinetic modeling in the solvent phase. Both experimental and computational methods for calculating reaction rates in liquids are reviewed. Previous studies, which used such methods to determine solvent effects, are then analyzed based on reaction family. Many of these studies correlate kinetic solvent effect with one or more solvent parameters or properties of reacting species, but it is not always possible, and investigations are usually done on too few reactions and solvents to truly generalize. From these studies, we present suggestions on how best to use data to generalize solvent effects for many different reaction types in a high throughput manner. </p> </div> </div> </div>

A centrifuge-based stepwise chemical loading disc for the production of multiplex anisotropic metallic nanoparticles

RSC Advances ◽  
2015 ◽  
Vol 5 (3) ◽  
pp. 1846-1851 ◽  
Author(s):  
Byung Hyun Park ◽  
Ji Hyun Lee ◽  
Jae Hwan Jung ◽  
Seung Jun Oh ◽  
Doh C. Lee ◽  
...  
Keyword(s):  
High Throughput ◽  
Metallic Nanoparticles ◽  
Au Nps ◽  
High Throughput Manner ◽  
Chemical Loading

We have proposed a novel rotary microdevice in which multiplex anisotropic Au NPs could be synthesized under diverse conditions in a high-throughput manner.

scholarly journals Novel High-Throughput Detection Method To Assess Bacterial Surfactant Production

2010 ◽  
Vol 76 (16) ◽  
pp. 5363-5372 ◽  
Author(s):  
Adrien Y. Burch ◽  
Briana K. Shimada ◽  
Patrick J. Browne ◽  
Steven E. Lindow
Keyword(s):  
Pseudomonas Syringae ◽  
High Throughput ◽  
Water Drop ◽  
Bacterial Strains ◽  
Swarming Motility ◽  
Detection Assay ◽  
Wide Range ◽  
Surfactant Production ◽  
Transposon Mutants ◽  
High Throughput Detection

ABSTRACT A novel biosurfactant detection assay was developed for the observation of surfactants on agar plates. By using an airbrush to apply a fine mist of oil droplets, surfactants can be observed instantaneously as halos around biosurfactant-producing colonies. This atomized oil assay can detect a wide range of different synthetic and bacterially produced surfactants. This method could detect much lower concentrations of many surfactants than a commonly used water drop collapse method. It is semiquantitative and therefore has broad applicability for uses such as high-throughput mutagenesis screens of biosurfactant-producing bacterial strains. The atomized oil assay was used to screen for mutants of the plant pathogen Pseudomonas syringae pv. syringae B728a that were altered in the production of biosurfactants. Transposon mutants displaying significantly altered surfactant halos were identified and further analyzed. All mutants identified displayed altered swarming motility, as would be expected of surfactant mutants. Additionally, measurements of the transcription of the syringafactin biosynthetic cluster in the mutants, the principal biosurfactant known to be produced by B728a, revealed novel regulators of this pathway.

scholarly journals Mapping of transgenic alleles in soybean using a nanopore-based sequencing strategy

2019 ◽  
Vol 70 (15) ◽  
pp. 3825-3833 ◽  
Author(s):  
Shengjun Li ◽  
Shangang Jia ◽  
Lili Hou ◽  
Hanh Nguyen ◽  
Shirley Sato ◽  
...  
Keyword(s):  
High Throughput ◽  
Soybean Genome ◽  
Transgenic Technology ◽  
Transgenic Soybean ◽  
Pooled Dna ◽  
Sequencing Strategy ◽  
Ds Element ◽  
Soybean Plants ◽  
Cost Efficient ◽  
Transgene Insertion

Abstract Transgenic technology was developed to introduce transgenes into various organisms to validate gene function and add genetic variations >40 years ago. However, the identification of the transgene insertion position is still challenging in organisms with complex genomes. Here, we report a nanopore-based method to map the insertion position of a Ds transposable element originating in maize in the soybean genome. In this method, an oligo probe is used to capture the DNA fragments containing the Ds element from pooled DNA samples of transgenic soybean plants. The Ds element-enriched DNAs are then sequenced using the MinION-based platform of Nanopore. This method allowed us to rapidly map the Ds insertion positions in 51 transgenic soybean lines through a single sequencing run. This strategy is high throughput, convenient, reliable, and cost-efficient. The transgenic allele mapping protocol can be easily translated to other eukaryotes with complex genomes.

scholarly journals High-Throughput PCR Assays To Monitor Wolbachia Infection in the Dengue Mosquito (Aedes aegypti) and Drosophila simulans

2012 ◽  
Vol 78 (13) ◽  
pp. 4740-4743 ◽  
Author(s):  
Siu F. Lee ◽  
Vanessa L. White ◽  
Andrew R. Weeks ◽  
Ary A. Hoffmann ◽  
Nancy M. Endersby
Keyword(s):  
Aedes Aegypti ◽  
High Throughput ◽  
Large Scale ◽  
Wolbachia Infection ◽  
Drosophila Simulans ◽  
Content Type ◽  
Pcr Assays ◽  
Cost Efficient

ABSTRACTWe have developed and validated two new fluorescence-based PCR assays to detect theWolbachia wMel strain inAedes aegyptiand thewRi andwAu strains inDrosophila simulans. The new assays are accurate, informative, and cost-efficient for large-scaleWolbachiascreening.

scholarly journals Multiplex detection of miRNAs based on aggregation-induced emission luminogen encoded microspheres

RSC Advances ◽  
2019 ◽  
Vol 9 (68) ◽  
pp. 39976-39985 ◽  
Author(s):  
Dan Zou ◽  
Weijie Wu ◽  
Jingpu Zhang ◽  
Qiang Ma ◽  
Sisi Fan ◽  
...  
Keyword(s):  
Multiplex Detection ◽  
Aggregation Induced Emission ◽  
Detection Assay

A miRNA multiplex detection assay based on aggregation-induced emission luminogen encoded microspheres.

scholarly journals A nanozyme tag enabled chemiluminescence imaging immunoassay for multiplexed cytokine monitoring

2018 ◽  
Vol 54 (98) ◽  
pp. 13813-13816 ◽  
Author(s):  
Yihong Zhong ◽  
Xiao Tang ◽  
Juan Li ◽  
Qingchun Lan ◽  
Lingfeng Min ◽  
...  
Keyword(s):  
High Throughput ◽  
Multiplex Detection

We propose a new concept of a chemiluminescence imaging nanozyme immunoassay (CINIA), in which nanozymes are exploited as catalytic tags for simultaneous and high-throughput multiplex detection of cytokines.

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