Immunocytochemical localization of Lewis blood group antigens in human salivary glands.

1994 ◽  
Vol 42 (8) ◽  
pp. 1135-1142 ◽  
Author(s):  
M Cossu ◽  
M S Lantini ◽  
R Puxeddu
Keyword(s):  
Salivary Glands ◽  
Blood Group ◽  
Secretory Granules ◽  
Ultrastructural Level ◽  
Blood Group Antigens ◽  
Immunogold Staining ◽  
Secretor Status ◽  
Duct Cells ◽  
Granular Matrix ◽  
B Blood Group

We demonstrated the immunohistochemical distribution of Le-a and Le-b blood group antigens in human major and minor salivary glands at the ultrastructural level by applying a post-embedding immunogold staining method. In secretors' glands, a faint Le-a reactivity was found only in mucous droplets, whereas Le-b antigen was intensely stained in secretory granules of most mucous cells, in those of intercalated duct cells, in the pale granular matrix of some serous cells, and, when osmication was omitted, in cytoplasmatic vesicles and cell surfaces of striated ducts. In the submandibular gland of a non-secretor, Le-a antigen was considerably stained in mucous droplets, whereas Le-b reactivity was restricted to the striated duct cells. These results indicate that the secretor status affects the secretion of Lewis antigens by mucous, serous, and intercalated duct cells but not the presence of Le-b as a surface antigen in striated duct cells.

scholarly journals THE HISTOLOGICAL DISTRIBUTION OF BLOOD GROUP SUBSTANCES A AND B IN MAN

1960 ◽  
Vol 111 (6) ◽  
pp. 785-800 ◽  
Author(s):  
Aron E. Szulman
Keyword(s):  
Blood Group ◽  
Fluorescent Antibody ◽  
Sweat Glands ◽  
Surgical Removal ◽  
Positive Staining ◽  
Fluorescent Antibody Technique ◽  
Blood Group Antigens ◽  
Specific Staining ◽  
Antibody Technique ◽  
Secretor Status

The mapping out of the histologic distribution of blood group antigens A and B in human tissues was performed by means of the fluorescent antibody technique. Human hyperimmune sera were conjugated with fluorescein isocyanate and applied to frozen sections of human material obtained at autopsy or after surgical removal. The material examined encompassed A, B, and AB subjects. In the latter the anti-A and the anti-B conjugate elicited the same picture. Group O tissues were used for controls and were uniformly negative. The secretor status of subjects was determined from the saliva or by the Lewis typing of erythrocytes. The results fall into the following main divisions: Endothelia of Vessels.—Widespread localization was demonstrated in the cell walls of endothelium of capillaries, veins, arteries, and of sinusoidal cells of spleen. Stratified Epithelia.—These showed good outlining of cells of the Malpighian (and the granular, when present) layers. In transitional epithelia, cells of the basal and contiguous layers gave specific staining. Mucus-Secreting Apparatus.—Positive staining was obtained in glands, goblet cells, and secreting surface epithelia. In non-secretors there was no identifiable antigen with the important exception of the deeper parts of gastric foveolae, deeper parts of crypts of Lieberkühn of bowel mucosa and Brunner's glands of the duodenum. Various Organs of Secretion and Excretion.—The pancreas (exocrine portion) and the sweat glands were found to produce the antigen irrespectively of secretor status. Breast, prostate, and endometrial glands on the other hand apparently secrete the antigen in conformity with the subject's secretor:non-secretor make-up. Thus the secretor:non-secretor status governs principally the antigens associated with mucous secretions and this in most but not all locations. The possible nature of this control is briefly discussed.

Role of Lewis A and Lewis B Blood Group Antigens in Helicobacter pylori Infection

Helicobacter ◽  
2004 ◽  
Vol 9 (4) ◽  
pp. 324-329 ◽  
Author(s):  
Dietrich Rothenbacher ◽  
Maria Weyermann ◽  
Gunter Bode ◽  
Murrat Kulaksiz ◽  
Bernd Stahl ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Blood Group ◽  
Helicobacter Pylori Infection ◽  
Blood Group Antigens ◽  
B Blood Group ◽  
Lewis A

Cell surface glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of Lactobacillus plantarum LA 318 recognizes human A and B blood group antigens

2008 ◽  
Vol 159 (9-10) ◽  
pp. 685-691 ◽  
Author(s):  
Hideki Kinoshita ◽  
Nozomi Wakahara ◽  
Masamichi Watanabe ◽  
Tomomi Kawasaki ◽  
Hiroki Matsuo ◽  
...  
Keyword(s):  
Cell Surface ◽  
Lactobacillus Plantarum ◽  
Blood Group ◽  
Blood Group Antigens ◽  
B Blood Group

Expression of A and B Blood Group Antigens on Cryopreserved Homografts

2009 ◽  
Vol 87 (1) ◽  
pp. 211-214 ◽  
Author(s):  
Brian Feingold ◽  
Peter D. Wearden ◽  
Victor O. Morell ◽  
Daniel Galvis ◽  
Csaba Galambos
Keyword(s):  
Blood Group ◽  
Blood Group Antigens ◽  
B Blood Group

scholarly journals Effect of Donor and Recipient ABH-Secretor Status on ABO-Incompatible Living Donor Kidney Transplantation

2021 ◽  
Vol 12 ◽  
Author(s):  
Fan Zhang ◽  
Saifu Yin ◽  
Yu Fan ◽  
Turun Song ◽  
Zhongli Huang ◽  
...  
Keyword(s):  
Kidney Transplantation ◽  
Blood Group ◽  
Graft Rejection ◽  
Living Donor ◽  
Blood Group Antigens ◽  
Post Transplantation ◽  
Donor Kidney ◽  
Secretor Status ◽  
Donor Kidney Transplantation ◽  
Living Donor Kidney

IntroductionABO blood group antigens within grafts are continuously exposed to anti-A/B antibodies in the serum of recipients after ABO-incompatible (ABOi) kidney transplantation and are instrumental in antibody-mediated rejection. Some individuals secrete soluble blood group antigens into body fluids. In this study, we investigated the effect of donor and recipient secretor status on the outcomes of ABOi kidney transplantation.MethodsData of a total of 32 patients with ABOi living donor kidney transplantation were retrospectively collected between 2014 and 2020 in West China Hospital. The genotype and phenotype of both donors and recipients were examined and evaluated with post-transplantation anti-A/B titer changes, graft function, and rejection.ResultsOf the 32 recipients and 32 donors, 23 (71.9%) recipients and 27 (84.4%) donors had secretor genotypes, whereas 9 (28.1%) recipients and 5 (15.6%) donors did not. Anti-A/B titers after ABOi kidney transplantation were not significantly influenced by the secretor status of either donors or recipients. The post-transplantation serum creatinine (Scr) levels and estimated glomerular filtration rate (eGFR) was better in weak- or non-secretor recipients at day 30 (Scr P = 0.047, eGFR P = 0.008), day 90 (Scr P = 0.010, eGFR P = 0.005), and month 9 (eGFR P = 0.008), and recipients from secretor donors had a lower incidence of graft rejection in the first year after ABOi transplantation (P = 0.004).ConclusionsA weak secretor status phenotype was found in both genotypes, i.e., individuals who secreted soluble antigens as well as those who did not. The recipient ABH-secretor status may have an influence on early posttransplant renal function, and the donor ABH-secretor status might affect the incidence of graft rejection.

scholarly journals Association between secretor status and Lewis phenotype with seronegative spondyloarthritis as indicator of autoimmunity

Genetika ◽  
2020 ◽  
Vol 52 (1) ◽  
pp. 127-136
Author(s):  
Ivan Busarcevic ◽  
Svetlana Vojvodic ◽  
Una Vojvodic
Keyword(s):  
Blood Group ◽  
Haemagglutination Inhibition ◽  
Gastrointestinal Symptoms ◽  
Blood Group Antigens ◽  
Control Subjects ◽  
Secretor Status ◽  
Lewis Blood Group ◽  
Increased Risk ◽  
Significant Difference ◽  
Seronegative Spondyloarthropathies

The classical paradigm of autoimmune pathogenesis involving specific genetic makeup and exposure to environmental triggers has been challenged recently by the addition of a third element, the loss of intestinal barrier function. Regardless of HLA B27 phenotype or gastrointestinal symptoms, evidence of ileitis, ileocolitis or colitis exists in patients with spondyloarthropathy. The FUT2 secretory gene is a strong candidate for Crohn's susceptibility by shaping the functional states of mucosal microbiota and may thus have influence on the release of zonulin, the main regulator of gut permeability. Gram negative bacteria precipitate and may be involved in the pathogenesis of spondyloarthropathies. Susceptibility to many infectious agents is associated with ABO blood group or secretor state. Patients who cannot secrete ABO and Lewis blood group antigens into body fluids, an ability controlled by a single gene on chromosome 19, are known to be at increased risk of certain autoimmune diseases associated with human leukocyte antigen (HLA) markers. Lewis (Le) blood group phenotype can be used to infer secretor status. The objective of this study was to determine the distribution of secretor state and Lewis blood group phenotype in patients with seronegative spondyloarthropathies and healthy control subjects. Hundred and ten (110) patients with seronegative spondyloarthropathies (58 females and 52 males) and 103 control (74 males and 29 females) subjects participated in this study. Samples of saliva and blood were subjected to haemagglutination inhibition tests for determination of secretor status and Lewis phenotype. A total of 92(84%) patients and 92 (89%) control subjects were secretors while 18 (16%) patients and 11 (11%) control subjects were non-secretors. There was no statistically significant difference (?2 1,461 p<0,05 and degrees of freedom 1) in distribution of secretor status in comparison to seronegative spondyloarthropathies by comparing two observed populations. Seven patients had modified (reduced) expression of Lewis b antigen on their erythrocytes. Reduction of Lewis b antigen expression was not observed on erythrocytes of healthy subjects. Reduced expression of Lewis b antigen could be a consequence of the inflammatory process within the gut and it also suggests several pathogenic mechanisms which may be relevant to the synthesis of Lewis antigens inside the gut or its absorption on erythrocytes in patients with spondyloarthropathy.

Lewis Blood Group Antigens in Salivary Glands and Stratified Epithelium: Lack of Regulation of Lewis Antigen Expression in Ductal and Buccal Mucosal Lining Epithelia

Vox Sanguinis ◽  
1991 ◽  
Vol 61 (3) ◽  
pp. 205-214
Author(s):  
Ulla Mandel ◽  
Torben F. Ørntoft ◽  
Eric H. Holmes ◽  
Henning Sørensen ◽  
Henrik Clausen ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Blood Group ◽  
Antigen Expression ◽  
Blood Group Antigens ◽  
Stratified Epithelium ◽  
Lewis Blood Group ◽  
Lewis Antigen ◽  
Mucosal Lining

scholarly journals Histochemical localization and analysis of blood group-related antigens in human pancreas using immunostaining with monoclonal antibodies and exoglycosidase digestion.

1990 ◽  
Vol 38 (9) ◽  
pp. 1331-1340 ◽  
Author(s):  
N Ito ◽  
K Nishi ◽  
M Nakajima ◽  
Y Okamura ◽  
T Hirota
Keyword(s):  
Blood Group ◽  
Acinar Cells ◽  
Cell Types ◽  
Human Pancreas ◽  
Secretor Status ◽  
Duct Cells ◽  
Different Cell Types ◽  
Centroacinar Cells ◽  
Precursor Chain

We examined the distribution of blood group-related antigens using an indirect immunoperoxidase method with monoclonal antibodies (MAb) directed to A, B, H, Lewis a (Lea), Lewis b (Leb), Lewis x (Lex), and Lewis y (Ley) antigens and Type 1 precursor chain in human pancreas. Effects of prior digestion with exoglycosidases on MAb stainings were simultaneously investigated. A, B, H, Leb, and Ley antigens were detected in acinar cells and interlobular duct cells but not in centroacinar cells, intercalated duct cells, and islet of Langerhans cells. The expression of these antigens in acinar cells was not dependent on Lewis type and secretor status of the tissue donors, whereas that in interlobular duct cells was strictly dependent on secretor status. The distribution pattern of these antigens in acinar cells was not homogeneous, i.e., cells producing H antigens expressed both Leb and Ley antigens but not A or B antigens, whereas those producing A or B antigens did not secrete Leb and Ley as well as H antigens. Digestion with alpha-N-acetylgalactosaminidase or alpha-galactosidase resulted in the appearance of Leb and Ley antigens as well as H antigen in acinar cells producing A and/or B antigens. Type 1 precursor chain was not detected in pancreatic tissues from secretors but appeared in acinar cells producing H antigen after alpha-L-fucosidase digestion, which also disclosed Lex but not Lea antigen in acinar cells expressing both Leb and Ley. In some non-secretors, MAb against Type 1 precursor chain reacted with acinar cells without enzyme digestion. Although Lea antigen was not detected in acinar cells, it was found in centroacinar cells, intercalated duct cells, and interlobular duct cells from all individuals examined except two Le(a-b-) secretors. After sialidase digestion, Lex antigen appeared in centroacinar and intercalated duct cells from some individuals. Sialidase digestion also elicited reactivity with MAb against Type 1 precursor chain in islet of Langerhans cells from some individuals. These results demonstrate the complexity in the pattern of expression and regulation of blood group-related antigens in different cell types of human pancreas. Such complexity may largely be ascribed to differences in individual genotypes and in gene expression patterns of different cell types.

scholarly journals Ultrastructural immunolocalization of kallikrein in apical granules of striated duct cells of cat submandibular gland.

1983 ◽  
Vol 31 (2) ◽  
pp. 345-347 ◽  
Author(s):  
M Schachter ◽  
G D Wheeler ◽  
R W Matthews ◽  
M W Peret ◽  
C Moriwaki
Keyword(s):  
Serine Protease ◽  
Submandibular Gland ◽  
Direct Evidence ◽  
Immune Reaction ◽  
Secretory Granules ◽  
Ultrastructural Level ◽  
Circumstantial Evidence ◽  
Duct Cells

Recent studies on the localization of the serine protease salivary kallikrein have led to the conclusion that it has a ductal localization and to the possibility that it is located there in small "secretory" granules. Until now, the latter inference has been based entirely on circumstantial evidence. In the present study, however, direct evidence from immunolocalization studies at the ultrastructural level establishes the localization of this enzyme in the apical duct granules in the cat submandibular gland. These granules stained only with immune sera to cat submandibular gland kallikrein and were the only subcellular structures that did so. They showed the "studded" appearance characteristic of the electron-dense aggregates of peroxidase-antiperoxidase seen in this type of immune reaction.

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