scholarly journals Novel whole blood assay for phenotyping platelet reactivity in mice identifies ICAM-1 as a mediator of platelet-monocyte interaction

Blood ◽  
2015 ◽  
Vol 126 (10) ◽  
pp. e11-e18 ◽  
Author(s):  
Paul C. J Armstrong ◽  
Nicholas S. Kirkby ◽  
Melissa V. Chan ◽  
Michaela Finsterbusch ◽  
Nancy Hogg ◽  
...  
Keyword(s):  
High Throughput ◽  
Whole Blood ◽  
Platelet Reactivity ◽  
Research Application ◽  
Whole Blood Assay ◽  
Blood Assay ◽  
Fibrinogen Binding ◽  
Key Points ◽  
Low Volume ◽  
Whole Blood Aggregometry

Key PointsLow-volume, high-throughput whole blood aggregometry will facilitate future mouse platelet function research. Application of this approach identifies ICAM-1 as a novel mediator of platelet-monocyte interaction through fibrinogen binding.

Platelet Reactivity in Patients on Aspirin and Clopidogrel Therapy Measured by a New Bedside Whole-Blood Assay

2019 ◽  
Vol 73 (1) ◽  
pp. 40-47 ◽  
Author(s):  
Amin Polzin ◽  
Carolin Helten ◽  
Lisa Dannenberg ◽  
Philipp Mourikis ◽  
David Naguib ◽  
...  
Keyword(s):  
Whole Blood ◽  
Platelet Reactivity ◽  
Whole Blood Assay ◽  
Blood Assay ◽  
Clopidogrel Therapy

scholarly journals Ultrafast and Predictive Mass Spectrometry–Based Autotaxin Assays for Label-Free Potency Screening

2017 ◽  
Vol 22 (4) ◽  
pp. 425-432 ◽  
Author(s):  
Tom Bretschneider ◽  
Andreas Harald Luippold ◽  
Helmut Romig ◽  
Daniel Bischoff ◽  
Klaus Klinder ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
Whole Blood ◽  
Label Free ◽  
Whole Blood Assay ◽  
Blood Assay ◽  
Data Points ◽  
Set Up

Autotaxin (ATX) is a promising drug target for the treatment of several diseases, such as cancer and fibrosis. ATX hydrolyzes lysophosphatidyl choline (LPC) into bioactive lysophosphatidic acid (LPA). The potency of ATX inhibitors can be readily determined by using fluorescence-based LPC derivatives. While such assays are ultra-high throughput, they are prone to false positives compared to assays based on natural LPC. Here we report the development of ultrafast mass spectrometry–based ATX assays enabling the measurement of data points within 13 s, which is 10 times faster than classic liquid chromatography–mass spectrometry. To this end, we set up a novel in vitro and whole-blood assay. We demonstrate that the potencies determined with these assays are in good agreement with the in vivo efficacy and that the whole-blood assay has the best predictive power. This high-throughput label-free approach paired with the translatable data quality is highly attractive for appropriate guidance of medicinal chemists for constructing strong structure-activity relationships.

scholarly journals Heterogeneity in neutrophil responses to immune complexes

2019 ◽  
Vol 3 (19) ◽  
pp. 2778-2789 ◽  
Author(s):  
Madelaine Duarte ◽  
Maragatha Kuchibhatla ◽  
Sanjay Khandelwal ◽  
Gowthami M. Arepally ◽  
Grace M. Lee
Keyword(s):  
Immune Complexes ◽  
Whole Blood ◽  
Neutrophil Activation ◽  
Whole Blood Assay ◽  
Blood Assay ◽  
Key Points

Key Points In a whole blood assay, ICs cause neutrophil activation and degranulation. Individuals have a fixed susceptibility to neutrophil activation by ICs.

scholarly journals A High Throughput Whole Blood Assay for Analysis of Multiple Antigen-Specific T Cell Responses in HumanMycobacterium tuberculosisInfection

2018 ◽  
Vol 200 (8) ◽  
pp. 3008-3019 ◽  
Author(s):  
Wendy E. Whatney ◽  
Neel R. Gandhi ◽  
Cecilia S. Lindestam Arlehamn ◽  
Azhar Nizam ◽  
Hao Wu ◽  
...  
Keyword(s):  
T Cell ◽  
High Throughput ◽  
Whole Blood ◽  
T Cell Responses ◽  
Whole Blood Assay ◽  
Cell Responses ◽  
Blood Assay ◽  
Multiple Antigen

Non-Thrombogenicity of Clean Glass Revealed by Native Whole Blood Assay in Bead Columns

1983 ◽  
Vol 50 (04) ◽  
pp. 814-820 ◽  
Author(s):  
J A Bergeron ◽  
J M DiNovo ◽  
A F Razzano ◽  
W J Dodds
Keyword(s):  
Whole Blood ◽  
Glass Bead ◽  
Factor Xii ◽  
Platelet Factor ◽  
Serotonin Content ◽  
Whole Blood Assay ◽  
Vascular Integrity ◽  
Blood Assay ◽  
Standard Contact ◽  
Recalcification Time

SummaryThe previously described native whole blood assay for materials in solution or suspension has been adapted to materials in a bead column configuration. These experiments showed that the glass itself accounts for little or none of the high blood-reactivity observed with conventional glass bead columns. Columns composed solely of soft glass that was “cleaned” by heat treatment (500-595° C 18 hr, electric oven) were benign toward flowing native whole blood for all variables measured (platelet count and platelet-free plasma [C14]-serotonin content, platelet factor 3 and factor XII activities, and recalcification time) with the standard contact protocol. In addition, the effluent successfully maintained perfusion of the isolated kidney, a measure of the ability of platelets to support vascular integrity. Prolonged (30 min) normothermic contact with titrated whole blood increased the subsequent reactivity of initially clean glass toward whole blood albeit to a level much less than that of conventional glass bead columns.

Whole Blood Assay: Thromboelastometry – Bleeding Management Algorithms

2021 ◽  
pp. 67-87
Author(s):  
Klaus Görlinger ◽  
James Iqbal ◽  
Daniel Dirkmann ◽  
Kenichi A. Tanaka
Keyword(s):  
Whole Blood ◽  
Whole Blood Assay ◽  
Bleeding Management ◽  
Blood Assay ◽  
Management Algorithms

scholarly journals Analysis of the dynamics of Staphylococcus aureus binding to white blood cells using whole blood assay and geno-to-pheno mapping

2020 ◽  
Vol 310 (3) ◽  
pp. 151411
Author(s):  
Daria Gaidar ◽  
Alice Jonas ◽  
Ruslan Akulenko ◽  
Ulla Ruffing ◽  
Mathias Herrmann ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Whole Blood ◽  
Blood Cells ◽  
White Blood Cells ◽  
Whole Blood Assay ◽  
Blood Assay
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