Time Interval from Last Chemotherapy to Stem Cell Collection Correlates with Peripheral Blood Absolute Lymphocyte Count at Apheresis and Survival Post-Autologous Stem Cell Transplantation in Non-Hodgkin’s Lymphoma.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 2927-2927 ◽  
Author(s):  
Shernan G. Holtan ◽  
Luis F. Porrata ◽  
David J. Inwards ◽  
Stephen A. Ansell ◽  
Ivana N.M. Micallef ◽  
...  
Keyword(s):  
Stem Cell ◽  
Peripheral Blood ◽  
Lymphocyte Count ◽  
Hodgkin’S Lymphoma ◽  
Group Versus ◽  
Absolute Lymphocyte Count ◽  
Hodgkin's Lymphoma ◽  
Time Interval ◽  
Non Hodgkin's Lymphoma ◽  
Stem Cell Collection

Abstract The infused autograft absolute lymphocyte count (A-ALC) is an independent prognostic factor for survival after autologous hematopoietic stem cell transplant (AHSCT) in non-Hodgkin’s lymphoma (NHL). Previous studies have shown that A-ALC directly correlates with the peripheral blood absolute lymphocyte count (PC-ALC) at the time of apheresis collection. However, factors affecting the PC-ALC at apheresis remain undefined. We hypothesized that one possible factor impacting PC-ALC may be the time interval from last chemotherapy to stem cell collection (TILC). Data from 160 patients who underwent AHSCT for treatment of relapsed NHL at Mayo Clinic between 1993 and 2001 were collected and analyzed. The primary end point of this study was correlation between TILC and PC-ALC, and our analysis revealed a strong correlation (r = 0.67, p < 0.0001). Further analysis revealed higher PC-ALC numbers in patients with TILC ≥ 60 days versus TILC < 60 days (median of 7.44 x 109/L in the ≥ 60 day group versus 3.87 x 109/L in the < 60 day group, p < 0.0001). Both the median overall survival (figure below) and the progression-free survival were longer in the TILC ≥ 60 days group versus the TILC < 60 days group (76 versus 21 months, p < 0.0037; 76 versus 11 months, p < 0.018, respectively). These findings were independent of other prognostic indicators for relapsed NHL patients undergoing APHSCT. The data supports our hypothesis that TILC affects PC-ALC and survival post-AHSCT in NHL. Figure Figure

G-CSF Treatment May Prevent Telomere Attrition in CD34+ After Peripheral Blood Autologous Stem Cell Transplantation: Shortening Time to engrafting but Early Relapse in Non Hodgkin's Lymphoma. First Experience without Stem Cell Cryopreservation.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 4358-4358
Author(s):  
Mario Silva-Moreno ◽  
Blanca Murillo-Ortiz ◽  
Martha Hernández-González ◽  
Sergio Solorio-Meza ◽  
Sandra Martínez-Garza ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Transplantation ◽  
Telomere Length ◽  
Cell Transplantation ◽  
Peripheral Blood ◽  
Hodgkin’S Lymphoma ◽  
Peripheral Blood Stem Cell ◽  
Hodgkin's Lymphoma ◽  
Non Hodgkin’S Lymphoma ◽  
Non Hodgkin's Lymphoma

Abstract Abstract 4358 Background Hematopoietic reconstitution after stem cell transplantation requires excessive replicative activity because of the limited number of stem cells that are used for transplantation. Telomere shortening has been detected in hematopoietic cells after allogenic peripheral blood stem cell transplantation (PBSCT). Recent studies have demonstrated shortening of telomeres after chemotherapy, this shortening being equivalent to 15-40 years of ageing which has been related to genetic instability, increase risk of mutations, myelodysplastic syndromes, secondary malignancies and relapse. Aims To measure in vivo the effect of G-CSF administration, on the regulation of stem cells obtained from peripheral blood collected for autologous transplantation and to know its possible implications, on telomere dynamics following high-dose (HD) chemotherapy courses cyclophosphamide (CY) and HD-Ara-C for relapsed non Hodgkin's lymphoma. Methods Telomeric measurement was performed with real-time polymerase chain reaction (RT-PCR),in peripheral blood progenitor cells(PBPC) collected on day 5 and 10 of G-CSF administration from patients with Lymphoma, and day 0,+7,+14 and +17 post transplantation. For CD34+ cells selection, mononuclear cells were isolated from peripheral blood stem cell by Ficoll-Hypaque. The purity of CD34 selected cells was determined in a cell sorter (Becton Dickinson) using a fluorescein isothiocyanate (FICT)-conjugate monoclonal antibody (mAb). Results We have shown dynamic telomere length change during the immediate reconstitution, Telomere Lenght (TL) was shorter in PBPC collected after HD-Ara-C and CY compared to TL after G-CSF administration, 6526bp vs. 4582 bp (P < 0.01). This result was confirmed on CD34+ cells. Engraftment of granulocytes and platelets come on day +13 and +19 respectively An increase in telomere length to day +19 (5962 bp) was found. Unfortunately we face with an early relapse of the same non Hodgkin's lymphoma at day +60. Conclusions Administration of G-CSF increased TL in CD34+ prevents telomere attrition after allogenic peripheral blood stem cell transplantation. The size and composition of the transplanted stem cell pool determines the ultimate telomere length. Whether PBSCT with shortened telomeres has engraftment improvement with G-CSF but risking early relapse warrants more prospective studies. We suggest that measurement of telomere length in peripheral blood collected for transplantation could be useful as a prognostic marker for engraftment/relapse in non Hodgkin's lymphoma without stem cell cryopreservation. Disclosures: No relevant conflicts of interest to declare.

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