Characterization of TPO Kinetics within 60 Days of Allogeneic Hematopoietic Stem Cell Transplantation and Its Correlation with Megakaryocyte Ploidy Distribution

Blood ◽  
2012 ◽  
Vol 120 (21) ◽  
pp. 4463-4463
Author(s):  
Xiao-hui Zhang ◽  
Hai-xia Fu ◽  
Dai-Hong Liu ◽  
Lan-Ping Xu ◽  
Kai-Yan Liu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell Transplantation ◽  
Stem Cell Transplantation ◽  
Distribution Pattern ◽  
Cell Transplantation ◽  
Hematopoietic Stem Cell ◽  
Flow Cytometric Analysis ◽  
Hematopoietic Stem ◽  
Platelet Recovery ◽  
Kinetics Of

Abstract Abstract 4463 Background: Thrombocytopenia is a critical complication following allogeneic hematopoietic stem cell transplantation (allo-HSCT), but its pathogenesis has remained obscure. Thrombopoietin (TPO) has been identified as a key cytokine for both megakaryogenesis and thrombopoiesis; however, the kinetics of TPO production after allo-HSCT has not been reported. This study characterized the kinetics of TPO and its correlation with the megakaryocyte ploidy distribution pattern within 60 days of allo-HSCT. Methods and Results: A total of 46 consecutive patients who underwent allo-HSCT from October 2008 to December 2008 were included in the study. The TPO levels and the ploidy distribution pattern of MKs were measured using ELISA and flow cytometric analysis, respectively. The results indicate that the TPO levels and the platelet counts followed opposite trends after allo-HSCT. Multivariate analysis indicate that endogenous TPO levels before allo-HSCT, and the number of transplanted CD34+ cells were significant predisposing factors for rapid platelet engraftment (P=0.010 and 0.007, respectively). Furthermore, we found a reduction of ploidy and an increase in immature MKs in patients with higher endogenous TPO levels (> 250 pg/ml) on day 60 after allo-HSCT. Moreover, lower TPO levels (≤250 pg/ml) on day 60 after allo-HSCT were associated with significantly improved 2-year OS (P=0.032) and reduced TRM (P=0.026). Conclusion: Our results suggest that increased endogenous TPO levels may not be sufficient after allo-HSCT, and that the decreased ability of TPO may account for the appearance of antibodies. The use of exogenous rhTPO may promote platelet recovery after allo-HSCT. However, these possibilities need to be examined further. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Tandem Autologous Hematopoietic Stem Cell Transplantation in Very Young Patients with Multiple Myeloma

2020 ◽  
Vol 09 (04) ◽  
pp. 233-235
Author(s):  
Rahul Naithani ◽  
Nitin Dayal ◽  
Reeta Rai
Keyword(s):  
Multiple Myeloma ◽  
Stem Cell ◽  
Hematopoietic Stem Cell Transplantation ◽  
Stem Cell Transplantation ◽  
Cell Transplantation ◽  
Hematopoietic Stem Cell ◽  
Young Patients ◽  
Cell Transplant ◽  
Hematopoietic Stem ◽  
Platelet Recovery

Abstract Introduction Multiple myeloma (MM) in very young patients is uncommon, and no treatment guidelines exist for these patients. Patients and Methods We performed a retrospective analysis of five very young myeloma patients who underwent tandem autologous hematopoietic stem cell transplantation (HSCT). Results The median age was 37 years (range = 34–40 years). A median of two leukapheresis was performed (range = 1–4). The median number of hematopoietic stem cells collected was 5.4 × 106/kg (4.4–8.2 × 106/kg). During first transplant, four patients received melphalan of 200 mg/m2 and one patient received melphalan of 140 mg/m2 (due to renal failure) as conditioning regimen. Second transplant conditioning was melphalan of 200 mg/m2 for one patient and melphalan of 140 mg/m2 for remaining four patients. Two patients were in complete remission, and two were in very good partial remission and one patient progressed to active disease at the time of tandem autologous bone marrow transplant. All patients developed significant mucositis. Neutrophil and platelet recovery was longer in tandem autologous hematopoietic stem cell transplant. More viral infections were seen in tandem transplant. Day 30 and day 100 mortality was nil. Conclusion We present data on tandem autologous HSCTs in very young patients with MM in India. Responses continued to improve in this small series.

Relationship of physical function to outcomes in autologous hematopoietic stem cell transplantation.

2019 ◽  
Vol 37 (31_suppl) ◽  
pp. 116-116 ◽  
Author(s):  
Joseph Caveney ◽  
Abraham Sebastian Kanate ◽  
Gerald Hobbs ◽  
Anne Swisher
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell Transplantation ◽  
Stem Cell Transplantation ◽  
Physical Function ◽  
Cell Transplantation ◽  
Hematopoietic Stem Cell ◽  
Hematopoietic Stem ◽  
Platelet Recovery ◽  
Neutrophil Recovery ◽  
Hospital Days

116 Background: Physical function is an important consideration for autologous hematopoietic stem cell transplantation (auto-HSCT) but it is often judged subjectively rather than tested. This study examined the ability of functional test results to predict key transplant outcomes versus subjective rating. Methods: Patients receiving outpatient auto-HSCT performed a pre-transplant 2-minute walk test (2MWT), isometric handgrip testing, 5-times sit-to-stand test (5XSST) and timed up and go test (TUG). Karnofsky Performance Status (KPS) score was assigned by the treating oncologist. At day 100 following auto-HSCT, charts were extracted for age, gender, diagnosis, total number of hospital days, and days of platelet and neutrophil recovery. Predictions were determined through stepwise regression analysis. Results: Data from 37 patients (24 M, 13 F) were analyzed. The most common diagnosis was multiple myeloma. One patient expired prior to day 100. KPS was weakly correlated with all functional tests (spearman rho values 0.15 to 0.27, p values 0.07 to 0.32). KPS did not predict # hospital days (p = 0.53), neurophil recovery (p = 0.22) or platelet recovery (p = 0.51). In contrast, there was a trend for 5XSTS to predict # hospital days (p = 0.10), although predictive ability was low (R2 = 0.07). TUG was the best predictor of platelet recovery (p = .021), followed by 2MWT (p = 0.23) (R2 = 0.10). KPS did not predict any outcome measure, and none of the variables predicted neutrophil recovery. Conclusions: KPS does not correlate well with physical function testing nor does it predict key auto-HSCT short-term outcomes. While not a major contributor to hospitalization days or blood count recovery time, 5XSST and TUG appear to have potential to predict transplant outcomes. Future testing with larger samples is needed to determine the impact of physical function on long-term auto-HSCT outcomes.

scholarly journals The Association of KIR2DS4 and Its Variant KIR1D with CMV Infection after HLA-Matched Sibling Hematopoietic Stem Cell Transplantation

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 2555-2555
Author(s):  
Yao Yao ◽  
Xiaoli Li ◽  
Liangjing Xu ◽  
Bin Liu ◽  
Yue Han ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell Transplantation ◽  
Stem Cell Transplantation ◽  
Cell Transplantation ◽  
Hematopoietic Stem Cell ◽  
Infection Rate ◽  
Cmv Infection ◽  
Hematopoietic Stem ◽  
Platelet Recovery ◽  
Matched Sibling

Abstract Objective To expolore the association of KIR2DS4 and its variant KIR1D with cytomegalovirus(CMV) infection after HLA-matched sibling hematopoietic stem cell transplantation. Methods Polymerase chain reaction with sequence-specific primers (PCR-SSP) method was used to genotype KIR genes in 267 donor-recipient pairs from Oct 2005 to Apr 2014. Posttransplant monitoring for CMV infection was performed by immune histochemically assays .165 donor-recipient pairs who belong to KIR gene haplotype AA were analyzed for the presence of KIR2DS4 and its variant KIR1D and then further subdivided into the following groups: 2DS4-/1D+ (homozygous for the deletion variant KIR1D), 2DS4+/1D+ (heterozygous), 2DS4+/1D- (two intact KIR2DS4 alleles). Furthermore, we investigated the influence of the KIR2DS4 variants on CMV infection of 165 patients receiving Sibling related HLA matched transplantation. Results There were no significant differences in frequency of KIR2DS4 or KIR1D between donors and recipients in the haplotype AA group. The ratio of 2DS4+ and KIR1D in haplotype AA group was 2:1.There was no difference on neutrophil engraftment and platelet recovery among the three groups after hematopoietic stem cell transplantation. The CMV infection rate was significantly higher in 2DS4+/1D- group compared with 2DS4+/1D+ group (44.0% vs 19.0%，P=0.002)．In 2DS4-/1D+ group ，the CMV infection rate was higer than that in 2DS4+KIR1D+ group (50.0% vs 19%，P=0.028). However，there was no difference in CMV infection rate between 2DS4+/1D-group and 2DS4-/1D+ group. Conclusion KIR2DS4 and its variant KIR1D are associated with CMV infection after HLA-matched sibling hematopoietic stem cell transplantation Disclosures No relevant conflicts of interest to declare.

Dendritic Cells Reconstitution after Different Allogeneic Hematopoietic Stem Cell Transplantation.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 5211-5211
Author(s):  
De Pei Wu ◽  
Junjie Cao ◽  
Caixia Li ◽  
Xiaojin Wu
Keyword(s):  
Dendritic Cells ◽  
Stem Cell ◽  
Hematopoietic Stem Cell Transplantation ◽  
Stem Cell Transplantation ◽  
Cell Transplantation ◽  
Hematopoietic Stem Cell ◽  
Hematopoietic Stem ◽  
Hla Dr ◽  
Significant Difference ◽  
Kinetics Of

Abstract Objective: To compare the dendritic cells reconstitution after different allogeneic hematopoietic stem cell transplantation in early time Methods: From June 2004 to March 2005, Twenty-eight patients undergoing allo-HSCT were enrolled in this study. There were 16 patients who undergone normal HSCT, 8 patients who undergone Haploidentical HSCT and 4 patients who undergone Nonmyeloablative HSCT. Three-colour flow cytometry was applied to study the alteration of the percentage and number in circulating peripheral blood dendritic cells subsets on day 14,day 30,day 60 after transplantation among three distinct type HSCT. Results: The dendritic cells subsets number of myeloablative HSCT patients were very low. No difference was observed in the kinetics of DC1 (Lin−HLA-DR+CD11c+)and DC2 (Lin−HLA-DR+CD123+) reconstitution between the normal HSCT group and Haploidentical HSCT group patients(p<0.05). There was significant difference between myeloablative HSCT group and normal healthy individuals(p<0.01). There was significantly different between the non-myeloablative HSCT group and the myeloablative HSCT group in the kinetics of DC1 and DC2 reconstitution(p<0.05). Conclusion: The early reconstitution of dendritic cells in Nonmyeloablative HSCT patients is earlier than the patients who undergone myeloablative HSCT. The early reconstitution of dendritic cells in normal HSCT and Haploidentical HSCT patients were later than others.

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