scholarly journals Correction of poor platelet transfusion responses with leukocyte-poor HL-A-matched platelet concentrates

Blood ◽  
1975 ◽  
Vol 46 (5) ◽  
pp. 743-750 ◽  
Author(s):  
RH Herzig ◽  
GP Herzig ◽  
MI Bull ◽  
JA Decter ◽  
HP Lohrmann ◽  
...  
Keyword(s):  
Blood Cells ◽  
Platelet Transfusion ◽  
White Blood Cells ◽  
Innocent Bystander ◽  
Platelet Concentrates ◽  
Simple Method ◽  
The Poor ◽  
Starting Point ◽  
Antigen Antibody ◽  
Transfusion Reactions

Abstract Matching donor-recipient pairs for HL-A antigens provides a logical starting point for selecting donors for recipients with extensive prior transfusion histories. However, during the course of continued exposure to even HL-A-matched platelet concentrates, further sensitization occurs, as indicated by progressively poorer post-transfusion increments and transfusion reactions. There is evidence that such sensitization may be due to non-HL-A antigens. Finally, it is postulated that the poor post-transfusion platelet increments obtained when standard platelet concentrates are used result from the leukoagglutinin antigen-antibody reaction involving the platelet as an “innocent bystander.” The standard platelet concentrate can be purified by a simple method of centrifugation (178 g times 3 min), removing about 96% of the contaminating white blood cells with concomitant loss of about 21% of the platelets. The use of these leukocyte-poor platelet concentrates can restore compatible transfusion increments in highly alloimmunized thrombocytopenic recipients. The luekocyte-poor concentrates can diminish undesirable transfusion reactions following imcompatible platelet transfusions.

scholarly journals Correction of poor platelet transfusion responses with leukocyte-poor HL-A-matched platelet concentrates

Blood ◽  
1975 ◽  
Vol 46 (5) ◽  
pp. 743-750 ◽  
Author(s):  
RH Herzig ◽  
GP Herzig ◽  
MI Bull ◽  
JA Decter ◽  
HP Lohrmann ◽  
...  
Keyword(s):  
Blood Cells ◽  
Platelet Transfusion ◽  
White Blood Cells ◽  
Innocent Bystander ◽  
Platelet Concentrates ◽  
Simple Method ◽  
The Poor ◽  
Starting Point ◽  
Antigen Antibody ◽  
Transfusion Reactions

Matching donor-recipient pairs for HL-A antigens provides a logical starting point for selecting donors for recipients with extensive prior transfusion histories. However, during the course of continued exposure to even HL-A-matched platelet concentrates, further sensitization occurs, as indicated by progressively poorer post-transfusion increments and transfusion reactions. There is evidence that such sensitization may be due to non-HL-A antigens. Finally, it is postulated that the poor post-transfusion platelet increments obtained when standard platelet concentrates are used result from the leukoagglutinin antigen-antibody reaction involving the platelet as an “innocent bystander.” The standard platelet concentrate can be purified by a simple method of centrifugation (178 g times 3 min), removing about 96% of the contaminating white blood cells with concomitant loss of about 21% of the platelets. The use of these leukocyte-poor platelet concentrates can restore compatible transfusion increments in highly alloimmunized thrombocytopenic recipients. The luekocyte-poor concentrates can diminish undesirable transfusion reactions following imcompatible platelet transfusions.

scholarly journals A Modified Counting Method for Very Small Number of Residual White Blood Cells in Platelet Concentrates by Flow Cytometory.

1997 ◽  
Vol 43 (1) ◽  
pp. 13-20
Author(s):  
Mitsunobu Tanaka ◽  
Taiko Seno ◽  
Noriko Okuni ◽  
Hirotoshi Shibata ◽  
Yoshio Kobayashi ◽  
...  
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Platelet Concentrates ◽  
Counting Method

Validation of blood counters for quality control of platelet concentrates with high platelet counts

2018 ◽  
Vol 42 (5) ◽  
pp. 201-204
Author(s):  
Nicole Arlt ◽  
Remo Rothe ◽  
Rainer Moog
Keyword(s):  
Quality Control ◽  
Peripheral Blood ◽  
Blood Cells ◽  
White Blood Cells ◽  
Detection Methods ◽  
Platelet Concentrates ◽  
Counting Method ◽  
European Guidelines ◽  
Coefficients Of Variation ◽  
Very High

AbstractBackgroundBlood counters are primarily used to measure peripheral blood cells including platelets (PLTs). In routine quality control of platelet concentrates (PCs), counters are also used to analyze very high PLT counts. To meet the requirements of national and European guidelines for quality assurance, the accuracy of counting very high PLT counts has to be validated. The aim of the present study was to validate four blood counters (one of which has two detection methods) focusing on the PLT count.MethodsThe comparison was performed with PCs using the blood counter devices CELL-DYN Ruby (optical count) and CELL-DYN Emerald (impedance count), Sysmex K-4500 (impedance count), Sysmex XN-550 (impedance count) and Sysmex XN-550 (optical count). For precision performances, samples were measured serially 5 times and the coefficients of variation were calculated and compared with manufacturers’ requirements. Additionally, 50 peripheral blood samples were analyzed and standard hemogram parameters (red blood cells [RBC], white blood cells [WBC], hemoglobin [HGB], hematocrit [HCT], PLTs) were compared.ResultsThe comparison showed significant differences between the studied blood counter devices in measuring high PLT counts. The CELL-DYN-Emerald, the Sysmex K-4500 and Sysmex XN-500 with the optical counting method measured significantly higher PLT counts compared to the CELL-DYN-Ruby and the Sysmex XN-500 with the impedance counting technology (p<0.0001) independent of their principle of measurement. The manufacturers provide comparable coefficients of variation. We achieved similar results for all counters. All results of the peripheral blood count parameters were comparable.ConclusionsOur study showed the importance of blood counter validations focusing on PCs with high PLT counts before routine use. Not only the generally fundamental method, but also the manufacturers’ peculiarities seem to play an important role.

A simple method of preparing white blood cells for filterability testing

2016 ◽  
Vol 6 (6) ◽  
pp. 635-639 ◽  
Author(s):  
J. Mikita ◽  
G. Nash ◽  
J. Dormandy
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Simple Method

Real-time amplification of HLA-DQA1 for counting residual white blood cells in filtered platelet concentrates

Transfusion ◽  
2004 ◽  
Vol 44 (9) ◽  
pp. 1314-1318 ◽  
Author(s):  
Tamimount Mohammadi ◽  
Henk W. Reesink ◽  
Christina M.J.E. Vandenbroucke-Grauls ◽  
Paul H.M. Savelkoul
Keyword(s):  
Real Time ◽  
Blood Cells ◽  
White Blood Cells ◽  
Platelet Concentrates ◽  
Hla Dqa1 ◽  
Time Amplification

Analisa Cross-Infection Virus AI Subtipe H5N1 Berdasarkan Imunoserologi pada Burung Air di Cagar Alam Pulau Dua

2013 ◽  
Vol 2 (2) ◽  
pp. 120
Author(s):  
Dewi Elfidasari ◽  
Riris Lindiawati Puspitasari
Keyword(s):  
Immune Response ◽  
Avian Influenza ◽  
Blood Cells ◽  
Hemagglutination Inhibition ◽  
White Blood Cells ◽  
Cross Infection ◽  
Domestic Poultry ◽  
Infectious Microorganism ◽  
Water Birds ◽  
Antigen Antibody

<p><em>Abstrak</em><em> </em><strong><em>-</em></strong><strong><em> </em></strong><strong>Imunoserologi adalah cara mengidentifikasi terbentuknya antibodi yang diproduksi oleh sel darah putih sebagai respon terhadap masuknya antigen. Salah satu teknik Imunoserologi yang lazim digunakan untuk mendeteksi keberadaan antibodi di dalam darah adalah uji hambatan hemaglutinasi (<em>Hemagglutination-inhibition</em>/HI). Pada uji ini digunakan antigen yang homolog sehingga akan terjadi ikatan antigen-antibodi. Titer antibodi merupakan salah satu indikator dalam menentukan respon imun organisme terhadap suatu infeksi, seperti infeksi Virus <em>Avian Influenza</em> (VAI) subtipe H5N1. Interaksi yang terjadi antara burung air liar dengan unggas domestik dapat menyebabkan <em>cross-infection</em>, baik dari unggas domestik ke burung liar maupun dari burung liar ke unggas domestik. Salah satunya cara yang dapat dilakukan untuk menentukan  pola penularan dan penyebaran VAI subtipe H5N1 pada kawasan CAPD adalah melalui analisa <em>cross-infection</em> berdasarkan imunoserologi dengan melihat titer antibodi yang terbentuk pada unggas (ayam, bebek, mentok) dan burung-burung air liar penetap di kawasan tersebut. Hasil penelitian ini menunjukkan bahwa <em>cross-infection</em> tidak terjadi pada penyebaran virus </strong><strong>V</strong><strong>AI subtipe H5N1 di kawasan CAPD. Penularan terjadi hanya satu arah, dari unggas domestik ke burung-burung air liar penetap di CAPD.</strong></p><p> </p><p><em>Abstract<strong> - Immunoserology is a method to identify the formation of antibodies that produced by white blood cells as a response to agains the antigen. One of Immunoserology assays technique that commonly used to detect the presence of antibodies is hemagglutination test (Hemagglutination-inhibition/HI). In this study we used homologous antigens to observed the antigen-antibody binding. The antibody titer is an indicator to determining the immune response for the infectious microorganism, such as Avian Influenza Virus (AIV) subtype H5N1. Interactions between wild water birds and domestic poultry can lead the cross-infection mechanism. The analysis of cross-infection by imunoserologi is one of the ways to find the patterns of transmission and spread of the AIV subtype H5N1 in CAPD. The results of this study was indicate that cross-infection did not occur in the spread of AIV subtype H5N1 in the CAPD. The mechanism of transmission was occurs by one direction, only from domestic poultry to wild water birds resident in CAPD.</strong></em></p>

Administration of platelet concentrates suspended in bicarbonated Ringer's solution in children who had platelet transfusion reactions

Vox Sanguinis ◽  
2017 ◽  
Vol 113 (2) ◽  
pp. 128-135 ◽  
Author(s):  
J. Kobayashi ◽  
R. Yanagisawa ◽  
T. Ono ◽  
Y. Tatsuzawa ◽  
Y. Tokutake ◽  
...  
Keyword(s):  
Platelet Transfusion ◽  
Platelet Concentrates ◽  
Ringer’S Solution ◽  
Transfusion Reactions

Platelet transfusion reactions do not occur more often in recipients transfused with apheresis versus buffy coat platelet concentrates

Transfusion ◽  
2016 ◽  
Vol 56 (12) ◽  
pp. 3144-3146 ◽  
Author(s):  
Christine Cserti-Gazdewich ◽  
Alioska Escorcia ◽  
Jacob Pendergrast ◽  
Lani Lieberman ◽  
Robert Skeate ◽  
...  
Keyword(s):  
Platelet Transfusion ◽  
Buffy Coat ◽  
Platelet Concentrates ◽  
Transfusion Reactions

scholarly journals Examination for Susceptibility of CAE

2004 ◽  
pp. 33-36
Author(s):  
Szilvia Kusza ◽  
Zsuzsanna Bősze ◽  
Sándor Kukovics ◽  
András Jávor
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Virus Genome ◽  
Microsatellite Analysis ◽  
Serological Tests ◽  
Caprine Arthritis Encephalitis Virus ◽  
Potential Source ◽  
Test Elisa ◽  
Starting Point ◽  
Serology Test

Caprine Arthritis Encephalitis (CAE) is a group of diseases in goats caused by a retrovirus, namely the Caprine Arthritis Encephalitis Virus. CAEV belongs to the lentivirus group within the retrovirus family. The CAEV is intimately associated with white blood cells, therfore any body secretions which contain white blood cells are a potential source of virus to other goats in the flock. Once goats are infected with CAEV, it remains infected for life and has to be eliminated.Since not all goats infected with CAEV progress to disease, very important to test goats for infection using a serology test (ELISA, AGID, IDT). These serological tests demonstrate the presence of antibodies to CAEV in goat serum or detect the virus genome in the white blood cells. However, Swiss results (Dolf and Ruff, 1994) pointed out individual and species differencies in predisposition for this disease. This result was considered as a starting point to our examination. Microsatellite analysis was used in order to find whether there was any association between genotype and serological results, and to look for a marker associated with this disease. To date, altogether 135 goats have been examined. Unfortunately, a significant association between serological results and genotype was not found using the Chi2 test

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