scholarly journals Lipid composition of freshly prepared and stored platelet concentrates

Blood ◽  
1980 ◽  
Vol 55 (1) ◽  
pp. 124-130 ◽  
Author(s):  
MA Hamid ◽  
TJ Kunicki ◽  
RH Aster
Keyword(s):  
Oleic Acid ◽  
Lipid Composition ◽  
Total Phospholipid ◽  
Fatty Acid Distribution ◽  
Phosphatidyl Inositol ◽  
Platelet Concentrates ◽  
Citrate Phosphate Dextrose ◽  
Acid Citrate Dextrose ◽  
Citrate Dextrose ◽  
Citrate Phosphate

To evaluate the possibility that changes in lipid composition might be related to the functional lesion that develops when platelets are stored as concentrates for several days, we measured lipid constituents of platelets in freshly prepared concentrates and in concentrates stored for 72 hr at 4 degrees C or at 20 degrees C under standard blood banking conditions. At 20 degrees C, but not at 4 degrees C, platelets lost about 15% of total cholesterol and 7%--11% of total phospholipid. The distribution of individual phospholipids remained unchanged. This was also true of the fatty acid distribution in total phospholipids and in individual phospholipids except for a statistically significant reduction of linoleic acid (18:2) and an increase in oleic acid (18:1) in phosphatidyl inositol (PI). Platelets collected in citrate-phosphate- dextrose (CPD) anticoagulant did not differ significantly in lipid composition from those collected in acid-citrate-dextrose (ACD) anticoagulant during the period of observation. These findings do not provide a basis to suggest that functional abnormalities developing in stored platelets are related to changes in lipid composition.

scholarly journals Lipid composition of freshly prepared and stored platelet concentrates

Blood ◽  
1980 ◽  
Vol 55 (1) ◽  
pp. 124-130 ◽  
Author(s):  
MA Hamid ◽  
TJ Kunicki ◽  
RH Aster
Keyword(s):  
Oleic Acid ◽  
Lipid Composition ◽  
Total Phospholipid ◽  
Fatty Acid Distribution ◽  
Phosphatidyl Inositol ◽  
Platelet Concentrates ◽  
Citrate Phosphate Dextrose ◽  
Acid Citrate Dextrose ◽  
Citrate Dextrose ◽  
Citrate Phosphate

Abstract To evaluate the possibility that changes in lipid composition might be related to the functional lesion that develops when platelets are stored as concentrates for several days, we measured lipid constituents of platelets in freshly prepared concentrates and in concentrates stored for 72 hr at 4 degrees C or at 20 degrees C under standard blood banking conditions. At 20 degrees C, but not at 4 degrees C, platelets lost about 15% of total cholesterol and 7%--11% of total phospholipid. The distribution of individual phospholipids remained unchanged. This was also true of the fatty acid distribution in total phospholipids and in individual phospholipids except for a statistically significant reduction of linoleic acid (18:2) and an increase in oleic acid (18:1) in phosphatidyl inositol (PI). Platelets collected in citrate-phosphate- dextrose (CPD) anticoagulant did not differ significantly in lipid composition from those collected in acid-citrate-dextrose (ACD) anticoagulant during the period of observation. These findings do not provide a basis to suggest that functional abnormalities developing in stored platelets are related to changes in lipid composition.

Preparation of optically clear lyophilized human serum for use in preparing control material.

1976 ◽  
Vol 22 (4) ◽  
pp. 456-460 ◽  
Author(s):  
G J Proksch ◽  
D P Bonderman
Keyword(s):  
Human Serum ◽  
Dextran Sulfate ◽  
Raw Materials ◽  
Divalent Metal ◽  
Divalent Metal Ions ◽  
Quality Control Material ◽  
Control Material ◽  
Acid Citrate Dextrose ◽  
Citrate Dextrose ◽  
Citrate Phosphate

Abstract We describe a simple, rapid process--which includes the specific precipitation of pre-beta and beta-lipoproteins with dextran sulfate and divalent metal ions--for preparing an optically clear human serum that retains its clarity upon reconstitution with water after having been frozen and lyophilized. Such serum contains the normal constituents of human serum, except for the removed lipoproteins. The process causes no apparent interference with results of analyses for 22 of the more commonly measured constituents. Fresh or aged pooled serum or blood-bank plasma containing acid-citrate-dextrose or citrate-phosphate-dextrose are equally suitable as raw materials. This stabilized serum is an excellent matrix for use in preparing standards and quality-control material for assay of components of human serum.

Studies on a ph related zone phenomenon in erythrocytes

1978 ◽  
Vol 36 (2) ◽  
pp. 296-297
Author(s):  
Alan H. Timme
Keyword(s):  
Alcian Blue ◽  
Room Temperature ◽  
Unit Membrane ◽  
Paired Samples ◽  
Cacodylate Buffer ◽  
Charged Membrane ◽  
Acid Citrate Dextrose ◽  
Citrate Dextrose ◽  
Citrate Phosphate ◽  
Positively Charged

In 1967 Baker reported the occurrence of a zone of dense material adjacent to the inner leaflet of the unit membrane of erythrocytes exposed to buffers with a pH 4.5. It was postulated that this arose because the haemoglobin, positively charged at that pH, was attracted to the negatively charged membrane.In an attempt to elucidate the mechanisms underlying the formation of the zone, fresh human erythrocytes were washed for 15 minutes at room temperature in citrate phosphate buffers in which the pH ranged from 4.0 - 7.4, or in a standard preservative solution, Acid Citrate Dextrose (ACD), pH 5.0. Paired samples were subsequently washed in buffer at pH 7.4 for 10 minutes to assess the possible reversibility of any changes produced. The cells were fixed in 2.5% glutaraldehyde in 0.1 m cacodylate buffer at the same pH as the washing buffer for one hour and then processed in two ways: Some were post-fixed in OSO4, The cells were stained with 0.5 - 1% Alcian Blue 8 G S in cacodylate buffer pH 7.0 for 15 - 30 minutes.

scholarly journals Changes in intracellular Mg adenosine triphosphate and ionized Mg2+ during blood storage: detection by 31P nuclear magnetic resonance spectroscopy

Blood ◽  
1985 ◽  
Vol 65 (6) ◽  
pp. 1526-1530 ◽  
Author(s):  
JL Bock ◽  
B Wenz ◽  
RK Gupta
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Adenosine Triphosphate ◽  
Resonance Spectroscopy ◽  
Blood Storage ◽  
31P Nuclear Magnetic Resonance ◽  
Acid Citrate Dextrose ◽  
Citrate Dextrose ◽  
Citrate Phosphate ◽  
Nuclear Magnetic

Abstract 31P nuclear magnetic resonance (NMR) spectroscopy was used to measure changes in intra-erythrocyte Mg adenosine triphosphate (MgATP) and free Mg2+ during blood storage at 4 degrees C in standard citrate preservation media. The extent of Mg2+ complexation of ATP and the concentration of free Mg2+ were measured from the Mg2+-dependent chemical shift differences, at 22 degrees C, between the P beta and P alpha resonances of intracellular ATP. This difference changed from 721.0 +/- 1.4 Hz (mean +/- SE) on the day of collection to 741.0 +/- 3.4 Hz after three to seven days and 774.0 +/- 2.8 Hz after 11 to 40 days storage in either acid-citrate-dextrose (ACD) or citrate-phosphate- dextrose-adenine (CPDA-1). Changes in intracellular pH, detected from shifts in the intracellular Pi resonance, averaged 0.27 units after 11 to 40 days of storage. These data indicate a sizable decrease in the extent of Mg2+ complexation of ATP, and a decrease by a factor of 2.6 in free Mg2+, during the shelf-life of blood stored in ACD or CPDA-1.

Density Increase and Ageing of Erythrocytes in Stored Blood

1989 ◽  
Vol 17 (5) ◽  
pp. 461-466 ◽  
Author(s):  
M. Rocchigiani ◽  
M. Pescaglini ◽  
S. Sestini ◽  
V. Micheli ◽  
C. Ricci
Keyword(s):  
Dehydrogenase Activity ◽  
Whole Blood ◽  
Storage Time ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Acid Citrate Dextrose ◽  
Citrate Dextrose ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Stored Blood ◽  
Citrate Phosphate

An increase in the density of erythrocytes was observed after storage of whole blood for 30 days at 4°C in either acid citrate – dextrose or citrate – phosphate – dextrose – adrenaline. Glucose- 6-phosphate dehydrogenase activity in unfractionated red blood cell lysates did not vary with the storage time. Enzyme activity in the lighter fraction separated by density gradient centrifugation was higher than that in heavier fractions. The decline in glucose-6-phosphate dehydrogenase activity with density was less marked after storage of whole blood for 30 days. It is suggested that density modifications are not related to the ageing of erythrocytes and additional mechanisms may be involved.

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