Genetically determined polymorphism of the circulating human breast cancer-associated DF3 antigen

Abstract The murine monoclonal antibody (MAb) DF3 was prepared against a human breast carcinoma. Previous studies have demonstrated that DF3 antigen levels are elevated in plasma of patients with breast cancer. Furthermore, MAb DF3 reacts with circulating glycoproteins of different molecular weights ranging from approximately 300 to 450 kd. The present study demonstrates that plasma DF3 antigen is comprised of at least four moieties with slow (S), intermediate (I), rapid (R) and very rapid (VR) electrophoretic mobilities. The electrophoretic mobility patterns for circulating DF3 antigen differ among individuals. Moreover, DF3 antigen is detectable in urine, and the electrophoretic mobility of the urinary moieties is similar, but not identical, to that in the plasma. Studies in family members suggest that the electrophoretic heterogeneity of plasma DF3 antigen is determined by codominant expression of multiple alleles at a single locus. This locus may code for the core protein of DF3 antigen. These findings thus identify a genetically determined polymorphism of a circulating tumor-associated glycoprotein.

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Genetically determined polymorphism of the circulating human breast cancer-associated DF3 antigen

Blood ◽

10.1182/blood.v71.2.436.bloodjournal712436 ◽

1988 ◽

Vol 71 (2) ◽

pp. 436-440 ◽

Cited By ~ 1

Author(s):

DF Hayes ◽

H Sekine ◽

D Marcus ◽

CA Alper ◽

DW Kufe

Keyword(s):

Breast Cancer ◽

Electrophoretic Mobility ◽

Core Protein ◽

Human Breast ◽

Mobility Patterns ◽

Multiple Alleles ◽

Molecular Weights ◽

The Core ◽

Electrophoretic Mobilities ◽

Genetically Determined

The murine monoclonal antibody (MAb) DF3 was prepared against a human breast carcinoma. Previous studies have demonstrated that DF3 antigen levels are elevated in plasma of patients with breast cancer. Furthermore, MAb DF3 reacts with circulating glycoproteins of different molecular weights ranging from approximately 300 to 450 kd. The present study demonstrates that plasma DF3 antigen is comprised of at least four moieties with slow (S), intermediate (I), rapid (R) and very rapid (VR) electrophoretic mobilities. The electrophoretic mobility patterns for circulating DF3 antigen differ among individuals. Moreover, DF3 antigen is detectable in urine, and the electrophoretic mobility of the urinary moieties is similar, but not identical, to that in the plasma. Studies in family members suggest that the electrophoretic heterogeneity of plasma DF3 antigen is determined by codominant expression of multiple alleles at a single locus. This locus may code for the core protein of DF3 antigen. These findings thus identify a genetically determined polymorphism of a circulating tumor-associated glycoprotein.

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Comparison of mammalian mitochondrial ribosomal ribonucleic acid from different species

Biochemical Journal ◽

10.1042/bj1281033 ◽

1972 ◽

Vol 128 (5) ◽

pp. 1033-1041 ◽

Cited By ~ 6

Author(s):

R. S. Mitra ◽

B. Bartoov ◽

J. Monahan ◽

K. B. Freeman

Keyword(s):

Ionic Strength ◽

Electrophoretic Mobility ◽

Density Gradients ◽

Molecular Weights ◽

Mitochondrial Rrna ◽

Electrophoretic Mobilities ◽

Ribosomal Ribonucleic Acid ◽

Rrna Species ◽

Low Ionic Strength ◽

Human And Mouse

Mitochondrial ribosomal RNA species from mouse L cells, rat liver, rat hepatoma, hamster BHK-21 cells and human KB cells were examined by electrophoresis on polyacrylamide–agarose gels and sedimentation in sucrose density gradients. The SE (electrophoretic mobility) and S values of mitochondrial rRNA of all species were highly dependent on temperature and ionic strength of the medium; the SE values increased and the S values decreased with an increase in temperature at a low ionic strength. At an ionic strength of 0.3 at 23–25°C or an ionic strength of 0.01 at 3–4°C the S and SE values were almost the same being about 16.2–18.0 and 12.3–13.6 for human and mouse mitochondrial rRNA. The molecular weights under these conditions were calculated to be 3.8×105–4.3×105 and 5.9×105–6.8×105, depending on the technique used. At 25°C in buffers of low ionic strength mouse mitochondrial rRNA species had a lower electrophoretic mobility than those of human and hamster. Under these conditions the smaller mitochondrial rRNA species of hamster had a lower electrophoretic mobility than that of human but the larger component had an identical mobility. Mouse and rat mitochondrial rRNA species had identical electrophoretic mobilities. Complex differences between human and mouse mitochondrial rRNA species were observed on sedimentation in sucrose density gradients under various conditions of temperature and ionic strength. Mouse L-cell mitochondrial rRNA was eluted after cytoplasmic rRNA on a column of methylated albumin–kieselguhr.

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An Investigation of the Effects of the Core Protein Telomerase Reverse Transcriptase on Wnt Signaling in Breast Cancer Cells

Molecular and Cellular Biology ◽

10.1128/mcb.00844-13 ◽

2013 ◽

Vol 34 (2) ◽

pp. 280-289 ◽

Cited By ~ 35

Author(s):

I. Listerman ◽

F. S. Gazzaniga ◽

E. H. Blackburn

Keyword(s):

Breast Cancer ◽

Reverse Transcriptase ◽

Wnt Signaling ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Core Protein ◽

Telomerase Reverse Transcriptase ◽

The Core

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In SituPhotolinked Nuclear Progesterone Receptors of Human Breast Cancer Cells: Subunit Molecular Weights after Transformation and Translocation*

Endocrinology ◽

10.1210/endo-113-6-2195 ◽

1983 ◽

Vol 113 (6) ◽

pp. 2195-2201 ◽

Cited By ~ 164

Author(s):

KATHRYN B. HORWITZ ◽

P. SUE ALEXANDER

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Human Breast Cancer ◽

Breast Cancer Cells ◽

Progesterone Receptors ◽

Human Breast Cancer Cells ◽

Human Breast ◽

Molecular Weights

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Virus-Like Particles in a Human Breast Cancer: Structural Similarity to Previously Reported Particles

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100072332 ◽

1973 ◽

Vol 31 ◽

pp. 378-379

Author(s):

G. Kasnic ◽

S. E. Stewart ◽

C. Urbanski

Keyword(s):

Breast Cancer ◽

Biological Activity ◽

Human Breast Cancer ◽

Osteogenic Sarcoma ◽

Human Tumor ◽

Structural Similarity ◽

Cell Tumor ◽

Human Breast ◽

Human Tumor Cell ◽

Type Particle

We have reported the maturation of an intracisternal A-type particle in murine plasma cell tumor cultures and three human tumor cell cultures (rhabdomyosarcoma, lung adenocarcinoma, and osteogenic sarcoma) after IUDR-DMSO activation. In all of these studies the A-type particle seems to develop into a form with an electron dense nucleoid, presumably mature, which is also intracisternal. A similar intracisternal A-type particle has been described in leukemic guinea pigs. Although no biological activity has yet been demonstrated for these particles, on morphologic grounds, and by the manner in which they develop within the cell, they may represent members of the same family of viruses.

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Further Ultrastructural Observations on Metastatic Nodules of Human Breast Cancer

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010010086x ◽

1968 ◽

Vol 26 ◽

pp. 224-225

Author(s):

John L. Swedo ◽

R. W. Talley ◽

John H. L. Watson

Keyword(s):

Breast Cancer ◽

Human Breast Cancer ◽

Estrogen Therapy ◽

Specimen Preparation ◽

Human Breast ◽

Autophagic Vacuoles ◽

Previous Communication ◽

Linear Profiles ◽

Metastatic Nodule

Since the report, which described the ultrastructure of a metastatic nodule of human breast cancer after estrogen therapy, additional ultrastructural observations, including some which are correlative with pertinent findings in the literature concerning mycoplasmas, have been recorded concerning the same subject. Specimen preparation was identical to that in.The mitochondria possessed few cristae, and were deteriorated and vacuolated. They often contained particulates and fibrous structures, sometimes arranged in spindle-shaped bundles, Fig. 1. Another apparent aberration was the occurrence, Fig. 2 (arrows) of linear profiles of what seems to be SER, which lie between layers of RER, and are often recognizably continuous with them.It was noted that the structure of the round bodies, interpreted as within autophagic vacuoles in the previous communication, and of vesicular bodies, described morphologically closely resembled those of some mycoplasmas. Specifically, they simulated or reflected the various stages of replication reported for mycoplasmas grown on solid nutrient. Based on this observation, they are referred to here as “mycoplasma-like” structures, in anticipation of confirmatory evidence from investigations now in progress.

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