scholarly journals Gamma/delta lineage relationship within a consecutive series of human precursor T-cell neoplasms

Blood ◽  
1989 ◽  
Vol 74 (7) ◽  
pp. 2508-2518 ◽  
Author(s):  
JP de Villartay ◽  
AB Pullman ◽  
R Andrade ◽  
E Tschachler ◽  
O Colamenici ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Consecutive Series ◽  
Gene Rearrangements ◽  
Gamma Delta ◽  
Delta Gene ◽  
Gene Usage ◽  
Rearrangement Process ◽  
Gamma Delta T Cell

Abstract We analyzed the gene rearrangements associated with the newly described delta T-cell receptor (TCR) gene from a series of 19 consecutive precursor T-cell (lymphoblastic) neoplasms that represent discrete stages surrounding the TCR gene rearrangement process. Significantly, the delta TCR gene showed rearrangement in most (13 of 19) of these T cells, and in addition it was rearranged in two cells displaying no rearrangement for any other TCR gene. Our survey showed three types of delta gene rearrangements associated with cell-surface TCR expression that presumably represent usage of three V delta genes. This analysis demonstrates (1) a major subclass of human precursor T-cell neoplasms belonging to the gamma/delta T-cell receptor-rearranging subtype; (2) a narrow repertoire of human V delta gene usage; and (3) the utility of delta gene rearrangements as a diagnostic clonal marker in precursor T lymphoblastic neoplasms.

scholarly journals Gamma/delta lineage relationship within a consecutive series of human precursor T-cell neoplasms

Blood ◽  
1989 ◽  
Vol 74 (7) ◽  
pp. 2508-2518
Author(s):  
JP de Villartay ◽  
AB Pullman ◽  
R Andrade ◽  
E Tschachler ◽  
O Colamenici ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Consecutive Series ◽  
Gene Rearrangements ◽  
Gamma Delta ◽  
Delta Gene ◽  
Gene Usage ◽  
Rearrangement Process ◽  
Gamma Delta T Cell

We analyzed the gene rearrangements associated with the newly described delta T-cell receptor (TCR) gene from a series of 19 consecutive precursor T-cell (lymphoblastic) neoplasms that represent discrete stages surrounding the TCR gene rearrangement process. Significantly, the delta TCR gene showed rearrangement in most (13 of 19) of these T cells, and in addition it was rearranged in two cells displaying no rearrangement for any other TCR gene. Our survey showed three types of delta gene rearrangements associated with cell-surface TCR expression that presumably represent usage of three V delta genes. This analysis demonstrates (1) a major subclass of human precursor T-cell neoplasms belonging to the gamma/delta T-cell receptor-rearranging subtype; (2) a narrow repertoire of human V delta gene usage; and (3) the utility of delta gene rearrangements as a diagnostic clonal marker in precursor T lymphoblastic neoplasms.

scholarly journals Amino acid substitutions in the floor of the putative antigen-binding site of H-2T22 affect recognition by a gamma delta T-cell receptor

1993 ◽  
Vol 90 (23) ◽  
pp. 11396-11400 ◽  
Author(s):  
S Moriwaki ◽  
B S Korn ◽  
Y Ichikawa ◽  
L van Kaer ◽  
S Tonegawa
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Peptide Transporter ◽  
Affect Recognition ◽  
Antigen Binding ◽  
In Vitro Mutagenesis ◽  
Gamma Delta ◽  
T Cell Clone ◽  
Gamma Delta T Cell

We have previously identified a self-reactive gamma delta T-cell clone (KN6) specific for the H-2T region gene product T22b. Now we have investigated by an in vitro mutagenesis analysis of the T22b gene the possibility that the interaction between the KN6 gamma delta T-cell receptor and T22b involves a peptide. The results demonstrate that mutations at the floor of the putative antigen-binding groove of T22b affect recognition by the gamma delta T-cell receptor. Furthermore, we have shown that KN6 cells react with cells that are deficient in the class I peptide transporter TAP1/TAP2. These results suggest that peptide is involved in the interaction of the KN6 T-cell receptor with T22 and that loading of T22 with the putative peptide is TAP1/TAP2-independent.

scholarly journals Secretion of a soluble, chimeric gamma delta T-cell receptor-immunoglobulin heterodimer.

1992 ◽  
Vol 89 (15) ◽  
pp. 6871-6875 ◽  
Author(s):  
D. Eilat ◽  
G. E. Kikuchi ◽  
J. E. Coligan ◽  
E. M. Shevach
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Gamma Delta ◽  
Gamma Delta T Cell

Identification of a T3-associated gamma delta T cell receptor on Thy-1+ dendritic epidermal Cell lines

Science ◽  
1987 ◽  
Vol 236 (4803) ◽  
pp. 834-837 ◽  
Author(s):  
F Koning ◽  
G Stingl ◽  
W. Yokoyama ◽  
H Yamada ◽  
W. Maloy ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Lines ◽  
Epidermal Cell ◽  
Cell Receptor ◽  
Gamma Delta ◽  
Gamma Delta T Cell

scholarly journals Enhancer-dependent and -independent steps in the rearrangement of a human T cell receptor delta transgene.

1994 ◽  
Vol 179 (1) ◽  
pp. 43-55 ◽  
Author(s):  
P Lauzurica ◽  
M S Krangel
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
T Cell Receptor ◽  
Gene Rearrangement ◽  
T Cell Development ◽  
Cell Receptor ◽  
Cell Development ◽  
Gamma Delta ◽  
Delta Gene ◽  
Alpha Beta

The rearrangement and expression of T cell receptor (TCR) gene segments occurs in a highly ordered fashion during thymic ontogeny of T lymphocytes. To study the regulation of gene rearrangement within the TCR alpha/delta locus, we generated transgenic mice that carry a germline human TCR delta minilocus that includes V delta 1, V delta 2, D delta 3, J delta 1, J delta 3, and C delta segments, and either contains or lacks the TCR delta enhancer. We found that the enhancer-positive construct rearranges stepwise, first V to D, and then V-D to J. Construct V-D rearrangement mimics a unique property of the endogenous TCR delta locus. V-D-J rearrangement is T cell specific, but is equivalent in alpha/beta and gamma/delta T lymphocytes. Thus, either there is no commitment to the alpha/beta and gamma/delta T cell lineages before TCR delta gene rearrangement, or if precommitment occurs, it does not operate directly on TCR delta gene cis-acting regulatory elements to control TCR delta gene rearrangement. Enhancer-negative mice display normal V to D rearrangement, but not V-D to J rearrangement. Thus, the V-D to J step is controlled by the enhancer, but the V to D step is controlled by separate elements. The enhancer apparently controls access to J delta 1 but not D delta 3, suggesting that a boundary between two independently regulated domains of the minilocus lies between these elements. Within the endogenous TCR alpha/delta locus, this boundary may represent the 5' end of a chromatin regulatory domain that is opened by the TCR delta enhancer during T cell development. The position of this boundary may explain the unique propensity of the TCR delta locus to undergo early V to D rearrangement. Our results indicate that the TCR delta enhancer performs a crucial targeting function to regulate TCR delta gene rearrangement during T cell development.

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