scholarly journals Biochemical studies on red blood cells from a patient with the Inab phenotype (decay-accelerating factor deficiency)

Blood ◽  
1991 ◽  
Vol 78 (12) ◽  
pp. 3291-3297 ◽  
Author(s):  
ME Reid ◽  
G Mallinson ◽  
RB Sim ◽  
J Poole ◽  
V Pausch ◽  
...  
Keyword(s):  
Blood Group ◽  
Pcr Amplification ◽  
Phosphatidyl Inositol ◽  
Human Antibody ◽  
Blood Group Antigens ◽  
Decay Accelerating Factor ◽  
Reading Frame ◽  
Old Russian ◽  
Barr Virus ◽  
Epstein Barr

Abstract A 38-year-old Russian woman (KZ) has been identified as the fourth proposita with the Inab blood group phenotype. Like the first two propositi, she has a chronic intestinal disorder and, as shown for the third proposita, her Inab phenotype is demonstrably inherited. KZ's serum contained anti-IFC, which reacted with a red blood cell (RBC) membrane component with an Mr of 70,000, which is decay accelerating factor (DAF). Her RBCs lacked all Cromer-related blood group antigens and DAF. Her RBCs were no more susceptible than normal control RBCs to lysis in acid lysis or in rabbit or human antibody-initiated complement lysis tests. Northern blots of total RNA isolated from KZ's Epstein- Barr virus-transformed lymphoblasts showed a marked reduction of DAF mRNA when compared with normal. Polymerase chain reaction (PCR) amplification of cDNA confirmed this reduced level of DAF mRNA. Sequencing of the PCR product showed a 44-nucleotide deletion in the mRNA close to the short consensus repeats IIIa/IIIb intron/exon boundary. This deletion results in a change in the reading frame that places a termination codon six amino acids after the deletion. The putative translation product would lack a glycosyl phosphatidyl- inositol linkage site and, therefore, would not be membrane-bound in the RBC.

scholarly journals Biochemical studies on red blood cells from a patient with the Inab phenotype (decay-accelerating factor deficiency)

Blood ◽  
1991 ◽  
Vol 78 (12) ◽  
pp. 3291-3297 ◽  
Author(s):  
ME Reid ◽  
G Mallinson ◽  
RB Sim ◽  
J Poole ◽  
V Pausch ◽  
...  
Keyword(s):  
Blood Group ◽  
Pcr Amplification ◽  
Phosphatidyl Inositol ◽  
Human Antibody ◽  
Blood Group Antigens ◽  
Decay Accelerating Factor ◽  
Reading Frame ◽  
Old Russian ◽  
Barr Virus ◽  
Epstein Barr

A 38-year-old Russian woman (KZ) has been identified as the fourth proposita with the Inab blood group phenotype. Like the first two propositi, she has a chronic intestinal disorder and, as shown for the third proposita, her Inab phenotype is demonstrably inherited. KZ's serum contained anti-IFC, which reacted with a red blood cell (RBC) membrane component with an Mr of 70,000, which is decay accelerating factor (DAF). Her RBCs lacked all Cromer-related blood group antigens and DAF. Her RBCs were no more susceptible than normal control RBCs to lysis in acid lysis or in rabbit or human antibody-initiated complement lysis tests. Northern blots of total RNA isolated from KZ's Epstein- Barr virus-transformed lymphoblasts showed a marked reduction of DAF mRNA when compared with normal. Polymerase chain reaction (PCR) amplification of cDNA confirmed this reduced level of DAF mRNA. Sequencing of the PCR product showed a 44-nucleotide deletion in the mRNA close to the short consensus repeats IIIa/IIIb intron/exon boundary. This deletion results in a change in the reading frame that places a termination codon six amino acids after the deletion. The putative translation product would lack a glycosyl phosphatidyl- inositol linkage site and, therefore, would not be membrane-bound in the RBC.

EPSTEIN-BARR VIRUS INFECTION IN THALASSEMIA PATIENTS RELATED TO BLOOD GROUP IN MOSUL / IRAQ

2019 ◽  
Vol 22 (08) ◽  
pp. 136-145
Author(s):  
Anmar Ahmed Al Taie ◽  
Momammed Abdulrazzaq Ibraheem ◽  
Khansaa Basem Fadhil
Keyword(s):  
Virus Infection ◽  
Blood Group ◽  
Epstein Barr Virus ◽  
Epstein Barr Virus Infection ◽  
Barr Virus ◽  
Epstein Barr ◽  
Barr Virus Infection

scholarly journals One percent of human circulating B lymphocytes are capable of producing the natural anti-Gal antibody

Blood ◽  
1993 ◽  
Vol 82 (8) ◽  
pp. 2485-2493 ◽  
Author(s):  
U Galili ◽  
F Anaraki ◽  
A Thall ◽  
C Hill-Black ◽  
M Radic
Keyword(s):  
B Lymphocytes ◽  
Blood Group ◽  
Enzyme Linked Immunosorbent Assay ◽  
Barr Virus ◽  
Effective System ◽  
Group A ◽  
Igh Gene Rearrangement ◽  
Epstein Barr ◽  
B Lymphoid ◽  
Group B

The natural anti-Gal antibody constitutes 1% of circulating IgG in humans and interacts specifically with the carbohydrate epitope Gal alpha 1–3Gal beta 1–4GlcNAc-R (the alpha-galactosyl epitope). In view of the unusually large amounts of this antibody in the serum, it was of interest to determine the proportion of circulating B lymphocytes capable of synthesizing anti-Gal. For this purpose, blood B lymphocytes were incubated with Epstein-Barr virus (EBV) and plated in microtiter wells. Proliferation of the EBV transformed B lymphocytes was readily visible after 3 weeks of incubation. The supernatants from wells containing proliferating B-lymphoid clones were assayed for anti-Gal by an agglutination assay with rabbit red blood cells and the specificity of the agglutinating antibodies was further confirmed by their interaction with synthetic oligosaccharides and by enzyme-linked immunosorbent assay with glycoproteins. Approximately 5% of the wells contained anti-Gal antibodies. Limiting dilution studies and IgH gene rearrangement patterns suggested that each well contained an average of five proliferating B-lymphoid clones. Thus, it is concluded that approximately 1% of circulating B lymphocytes are capable of producing anti-Gal. The proportion of anti-Gal--producing lymphoid clones exceeds by fourfold that of clones producing anti-blood group A or anti-blood group B antibodies. Individual anti-Gal clones display fine variations in their combining site, as indicated by their differential interaction with alpha-galactosyl epitopes on glycolipids and on N-linked carbohydrate chains of glycoproteins. The high frequency of precursor B lymphocytes capable of producing anti-Gal, found in every individual and the restricted specificity of this antibody to alpha-galactosyl epitopes, potentially makes anti-Gal--producing lymphocytes an effective system for studying human Ig genes involved in the natural immune response to structurally defined haptens.

scholarly journals Unexpected Absence of the Epstein-Barr Virus (EBV) lyLMP-1 Open Reading Frame in Tumor Virus Isolates: Lack of Correlation between Met129 Status and EBV Strain Identity

2003 ◽  
Vol 77 (7) ◽  
pp. 4415-4422 ◽  
Author(s):  
Kimberly D. Erickson ◽  
Christoph Berger ◽  
William F. Coffin ◽  
Edwin Schiff ◽  
Dennis M. Walling ◽  
...  
Keyword(s):  
Cell Lines ◽  
Epstein Barr Virus ◽  
Open Reading Frame ◽  
Lytic Cycle ◽  
Reading Frame ◽  
Barr Virus ◽  
Tumor Virus ◽  
Epstein Barr ◽  
Virus Isolates ◽  
Codon 129

ABSTRACT The lytic cycle-associated lytic latent membrane protein-1 (lyLMP-1) of Epstein-Barr virus (EBV) is an amino-terminally truncated form of the oncogenic LMP-1. Although lyLMP-1 shares none of LMP-1's transforming and signal transducing activities, we recently reported that lyLMP-1 can negatively regulate LMP-1-stimulated NF-κB activation. The lyLMP-1 protein encoded by the B95-8 strain of EBV initiates from methionine 129 (Met129) of the LMP-1 open reading frame (ORF). The recent report that Met129 in the B95-8 LMP-1 ORF is not conserved in the Akata strain of EBV prompted us to screen a panel of EBV-positive cell lines for conservation of Met129 and lyLMP-1 expression. We found that 15 out of 16 tumor-associated virus isolates sequenced encoded an ATT or ACC codon in place of ATG in the LMP-1 ORF at position 129, and tumor cell lines harboring isolates lacking an ATG at codon 129 did not express the lyLMP-1 protein. In contrast, we found that EBV DNA from 22 out of 37 healthy seropositive donors retained the Met129 codon. Finally, the lyLMP-1 initiator occurs variably within distinct EBV strains and its presence cannot be predicted by EBV strain identity. Thus, Met129 is not peculiar to the B95-8 strain of EBV, but rather can be found in the background of several evolutionarily distinct EBV strains. Its absence from EBV isolates from tumors raises the possibility of selective pressure on Met129 in EBV-dependent tumors.

scholarly journals Rearrangements of the blood group RhD gene associated with the DVI category phenotype

Blood ◽  
1994 ◽  
Vol 83 (4) ◽  
pp. 1129-1135 ◽  
Author(s):  
I Mouro ◽  
C Le Van Kim ◽  
C Rouillac ◽  
DJ van Rhenen ◽  
PY Le Pennec ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Group ◽  
Blood Cells ◽  
Genomic Rearrangements ◽  
Blood Group Antigens ◽  
Reading Frame ◽  
Mrna Sequencing ◽  
Major Antigen ◽  
Rhesus Blood Group ◽  
Equivalent Region

Abstract The Rh (Rhesus) blood group antigens, D, Cc, and Ee, are carried by three unglycosylated membrane proteins of the human erythrocytes encoded by two highly related genes, D and CcEe. The major antigen, D, is a mosaic composed of at least nine determinants (epD1 through epD9). The lack of expression of some of these D epitopes at the surface of variant red blood cells defines the so-called D category phenotypes. In this report, we have determined the molecular basis of the DVI category phenotype characterized by the lack of epitopes D1, D2, D5, D6/7, and D8. Southern blot analysis and mRNA sequencing showed that the DVI phenotype is associated with two types of rearrangement of the D gene. Of 10 DVI genomes investigated, 8 exhibited a segmental DNA replacement (gene conversion) between the D fragment encompassing exons 4, 5, and 6 and the equivalent region of the CcEe gene. In the two other variants, these three exons are deleted. In both cases, the genomic rearrangement did not alter the reading frame of the variant RhD transcripts that are translated in 417 and 266 amino acid polypeptides, respectively. A heterogeneity of category DVI samples based on variable reactivity of the red blood cells with anti-D antibodies was previously found to be associated with the CDVIe or cDVIE haplotypes. Interestingly, our present results indicated that this serologic subdivision of the DVI category is correlated to two types of genomic rearrangements of the D gene.

scholarly journals The Late Lytic LMP-1 Protein of Epstein-Barr Virus Can Negatively Regulate LMP-1 Signaling

2000 ◽  
Vol 74 (2) ◽  
pp. 1057-1060 ◽  
Author(s):  
Kimberly D. Erickson ◽  
Jennifer M. Martin
Keyword(s):  
Epstein Barr Virus ◽  
Lytic Cycle ◽  
Dependent Manner ◽  
Reading Frame ◽  
Barr Virus ◽  
Tetradecanoyl Phorbol Acetate ◽  
Human B Cells ◽  
Negative Effect ◽  
Epstein Barr ◽  
Related Proteins

ABSTRACT The BNLF-1 open reading frame of Epstein-Barr virus (EBV) encodes two related proteins, latent membrane protein-1 (LMP-1) and lytic LMP-1 (lyLMP-1). LMP-1 is a latent protein required for immortalization of human B cells by EBV, whereas lyLMP-1 is expressed during the lytic cycle and is found in the EBV virion. We show here that, in contrast to LMP-1, lyLMP-1 is stable, with a half-life of >20 h in tetradecanoyl phorbol acetate- and butyrate-treated B95-8 cells. Although lyLMP-1 itself has negligible effects on NF-κB activity, it inhibits NF-κB activation by LMP-1 in a dose-dependent manner. The lyLMP-1 protein does not oligomerize with LMP-1, and the negative effect of lyLMP-1 on NF-κB activation by LMP-1 does not result from lyLMP-1-mediated disruption of LMP-1 oligomers. Modulation of LMP-1-activated signaling pathways is the first identified biological activity associated with lyLMP-1, and this activity may contribute to the progression of EBV's lytic cycle.

scholarly journals Use of Domain-Deletion Mutants to Locate Lutheran Blood Group Antigens to Each of the Five Immunoglobulin Superfamily Domains of the Lutheran Glycoprotein: Elucidation of the Molecular Basis of the Lua/Lub and the Aua/Aub Polymorphisms

Blood ◽  
1997 ◽  
Vol 89 (11) ◽  
pp. 4219-4225 ◽  
Author(s):  
S.F. Parsons ◽  
G. Mallinson ◽  
G.L. Daniels ◽  
C.A. Green ◽  
J.S. Smythe ◽  
...  
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
K562 Cells ◽  
Base Change ◽  
Immunoglobulin Superfamily ◽  
Deletion Mutants ◽  
Blood Group Antigens ◽  
Reading Frame ◽  
Polymerase Chain ◽  
Domain 3

Abstract Lutheran glycoprotein (Lu gp) has five predicted immunoglobulin superfamily (IgSF ) domains. K562 cells were transfected with Lu cDNA and tested by flow cytometry with monoclonal antibodies and Lu blood group antisera. The results confirmed the identity of Lu cDNA. Deletion mutants lacking the regions encoding one or more IgSF domains were made by inverse polymerase chain reaction (PCR), expressed in K562 cells, and tested with the same antibodies. The Lub and Lu5 antigens and the epitope recognized by monoclonal antibody BRIC 224 were mapped to the first, N-terminal, IgSF domain. Lu4 and Lu8 were mapped to domain 2; Lu20 to domain 3; Lu7 and BRIC 221 epitope to domain 4, and Lu13 and Aub to domain 5. The organization of the LU gene was determined. The region encoding the open reading frame is arranged in 15 exons extending over ≈11 kb on chromosome 19q13.2. The Lua/Lub and Aua/Aub blood group polymorphisms were studied using genomic DNA from typed blood donors. The Lua mutation is a base change in exon 3 (G252 to A) encoding an Arg77 (Lub) to His (Lua) change on the CFG face of domain 1. The Aua/Aub polymorphism is an A1637 to G substitution in exon 12 encoding a Thr539 (Aua) to Ala (Aub) change on the G strand of domain 5.

scholarly journals Pretreatment with Restriction Enzyme or Bovine Serum Albumin for Effective PCR Amplification of Epstein-Barr Virus DNA in DNA Extracted from Paraffin-Embedded Gastric Carcinoma Tissue

1998 ◽  
Vol 36 (11) ◽  
pp. 3423-3425 ◽  
Author(s):  
Yukio Satoh ◽  
Noriko Takasaka ◽  
Yoshiko Hoshikawa ◽  
Mitsuhiko Osaki ◽  
Satoshi Ohfuji ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Gastric Carcinoma ◽  
Restriction Enzyme ◽  
Bovine Serum ◽  
Epstein Barr Virus ◽  
Pcr Amplification ◽  
Barr Virus ◽  
Carcinoma Tissue ◽  
Epstein Barr ◽  
Gastric Carcinoma Tissue

An association between Epstein-Barr virus (EBV) and gastric carcinoma has been studied through the EBV genome present in the carcinoma cells. Recently, we found that EBV DNA in paraffin-embedded gastric carcinoma tissue was detected effectively by PCR after pretreatment of the extracted DNA with a restriction enzyme,BamHI or EcoRI. Here, we show that the PCR amplification was also enhanced by pretreatment of the DNA with other restriction enzymes or with bovine serum albumin and several other proteins. Treatment with these proteins may remove a PCR inhibitor(s) in the DNA samples extracted from the paraffin blocks.

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