A novel temporal expression pattern of three C/EBP family members in differentiating myelomonocytic cells

Members of the CCAAT/enhancer binding protein (C/EBP) family have been shown to regulate the terminal differentiation of adipocytes and hepatocytes. In these cell lineages, high levels of C/EBP alpha are found only in mature, nondividing cells. Using Western blotting and immunohistochemical staining, we have determined the temporal order of expression for C/EBP alpha, C/EBP beta, and C/EBP delta in differentiating myelomonocytic marrow cells. These studies show a unique temporal pattern of C/EBP isoform expression in the myeloid lineage. In particular, C/EBP alpha expression is very high in proliferative myelomonocytic cells, and diminishes during phenotypic maturation. While we have detected C/EBP alpha, C/EBP beta, and C/EBP delta in multiple myeloid leukemia cell lines, and C/EBP alpha in normal myeloid cells and in de novo human myeloid leukemias, we have not detected these C/EBP isoforms in either erythroid or lymphoid cells. Finally, we show that C/EBP alpha, C/EBP beta, and C/EBP delta protein and messenger RNA levels correlate in maturing granulocytic cells. The formation of tissue-specific combinations of C/EBP homodimers and heterodimers may allow this family of transcription factors to regulate different sets of genes in adipocytes, hepatocytes, and myelomonocytes.

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A novel temporal expression pattern of three C/EBP family members in differentiating myelomonocytic cells

Blood ◽

10.1182/blood.v80.7.1725.1725 ◽

1992 ◽

Vol 80 (7) ◽

pp. 1725-1735 ◽

Cited By ~ 279

Author(s):

LM Scott ◽

CI Civin ◽

P Rorth ◽

AD Friedman

Keyword(s):

Messenger Rna ◽

De Novo ◽

Leukemia Cell ◽

Lymphoid Cells ◽

Temporal Expression ◽

Leukemia Cell Lines ◽

Enhancer Binding Protein ◽

Myelomonocytic Cells ◽

Temporal Expression Pattern ◽

Myeloid Leukemia Cell

Abstract Members of the CCAAT/enhancer binding protein (C/EBP) family have been shown to regulate the terminal differentiation of adipocytes and hepatocytes. In these cell lineages, high levels of C/EBP alpha are found only in mature, nondividing cells. Using Western blotting and immunohistochemical staining, we have determined the temporal order of expression for C/EBP alpha, C/EBP beta, and C/EBP delta in differentiating myelomonocytic marrow cells. These studies show a unique temporal pattern of C/EBP isoform expression in the myeloid lineage. In particular, C/EBP alpha expression is very high in proliferative myelomonocytic cells, and diminishes during phenotypic maturation. While we have detected C/EBP alpha, C/EBP beta, and C/EBP delta in multiple myeloid leukemia cell lines, and C/EBP alpha in normal myeloid cells and in de novo human myeloid leukemias, we have not detected these C/EBP isoforms in either erythroid or lymphoid cells. Finally, we show that C/EBP alpha, C/EBP beta, and C/EBP delta protein and messenger RNA levels correlate in maturing granulocytic cells. The formation of tissue-specific combinations of C/EBP homodimers and heterodimers may allow this family of transcription factors to regulate different sets of genes in adipocytes, hepatocytes, and myelomonocytes.

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TheCDCREL1 gene fused toMLL in de novo acute myeloid leukemia with t(11;22)(q23;q11.2) and its frequent expression in myeloid leukemia cell lines

Genes Chromosomes and Cancer ◽

10.1002/1098-2264(2000)9999:9999<::aid-gcc1084>3.0.co;2-j ◽

2001 ◽

Vol 30 (3) ◽

pp. 230-235 ◽

Cited By ~ 13

Author(s):

Ken Tatsumi ◽

Tomohiko Taki ◽

Masafumi Taniwaki ◽

Hideo Nakamura ◽

Jun Taguchi ◽

...

Keyword(s):

Acute Myeloid Leukemia ◽

Cell Lines ◽

Myeloid Leukemia ◽

De Novo ◽

Leukemia Cell ◽

Leukemia Cell Lines ◽

Acute Myeloid ◽

Myeloid Leukemia Cell

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Study of Abnormal NHEJ Function in Human Myeloid Leukemia.

Blood ◽

10.1182/blood.v106.11.4349.4349 ◽

2005 ◽

Vol 106 (11) ◽

pp. 4349-4349

Author(s):

Zheng-zheng Fu ◽

Zi-xing Chen ◽

Zhi-min Wang2 ◽

Jian-nong Cen ◽

Jun He ◽

...

Keyword(s):

Cell Lines ◽

Plasmid Dna ◽

Myeloid Leukemia ◽

De Novo ◽

Leukemia Cell ◽

Leukemia Cells ◽

Leukemia Cell Lines ◽

The Mean ◽

Plasmid Puc18 ◽

Myeloid Leukemia Cell

Abstract Non-homologous end joining (NHEJ) is a major mechanism by which eukaryote cells can repair the DNA double-strand break (DSB) and protect the cell from further damages. Evidences have suggested that the genomic instability caused by deficient DNA repair function may contribute to the development of solid tumor, while its role in leukemogenesis has not been adequately studied. To study the NHEJ function in myeloid leukemia, an in vitro system was developed for clinical samples by using the linear plasmid pUC18 DNA digested with EcoR I as assay system.. Nuclear protein extracted from leukemic cells and mononuclear cells (MNCs) from normal BM or PB, was incubated with linear plasmid pUC18 DNA under certain conditions. Plasmid DNA was separated by agarose gel electrophoresis and imaged by SYBR greenIstaining. End-ligation efficiency was assessed by dividing the densitometry readings for the sum of all converted plasmid products by the sum of all products. This assay was performed on 7 myeloid leukemia cell lines, 16 samples of normal BM or PB cells, 20 cases of CML cells and 19 cases of de novo AML cells. E. coli strain DH5α was electrotransformed with pUC18 DNA end-joined by nuclear proteins from normal BM or leukemia cells, and was plated on LB agar, containing X-gal and IPTG. Correct ligation of cut plasmid DNA resulted in blue colonies while faulty repair would result in white colonies. The percentage of white colonies over total colonies gave the frequency of misrepair. Primers around the EcoR I site were designed and colony PCR was performed on blue and white colonies. Sequencing of PCR products was performed. Antibodies against the nuclear repair proteins Ku70, Ku86 and DNA-PKcs were used for antibody abrogation studies. We have found that the mean repair efficiency of normal MNCs was 18.6±13.1% (0~46.6%),. The ligation efficiencies in myeloid leukemic cell lines ranged 10.6%~31.0% (mean 22.4%, P>0.05). The mean ligation efficiency was significantly higher in CML cells as compared with normal MNCs (24.8±14.9% v.s 18.6%, p=0.024). The DNA repair capacity of de novo acute myeloid leukemia cells(7.2%~76.9%)was markedly increased as compared with normal MNCs (mean 41.1±15.4% v.s 18.6%, p<0.0001 ). The mean frequency of misrepair from AML and CML cells were considerable higher (AML, 8.17% and CML, 2.10%) than that of normal BM cells (0.91%). Most misrepair were small deletions near EcoR I site. Large deletions (>100bp) were found from assays with AML cells. Three abnormalities of sequence deletion, inversion and insertion were found in a rare misrepair white colony with AML cells. The DNA end-ligation efficiencies of AML cells could be dramatically inhibited by antibodies against proteins Ku70, Ku86 and DNA-PK. We concluded that the Cell-free NHEJ assay system can be used to examine the clinical leukemic samples. NHEJ efficiencies were slightly enhanced in most myeloid leukemia cell lines compared with normal MNCs. The end-ligation efficiency was significantly higher in primary myeloid leukemia cells. The overactive but more error-prone NHEJ function relied on the activity of Ku and DNA-PK proteins for DSB repair may contribute to genomic instability in AML cells.

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Isolation of a cDNA clone encoding a zinc finger protein highly expressed in T-leukemia lines

Blood ◽

10.1182/blood.v80.10.2571.2571 ◽

1992 ◽

Vol 80 (10) ◽

pp. 2571-2576 ◽

Cited By ~ 3

Author(s):

BY Wu ◽

EW Hanley ◽

LA Turka ◽

GJ Nabel

Keyword(s):

Zinc Finger ◽

Cdna Clone ◽

Messenger Rna ◽

Zinc Finger Protein ◽

Leukemia Cell ◽

Lymphoid Cells ◽

Gap Gene ◽

Finger Protein ◽

Leukemia Cell Lines

Abstract A cDNA clone encoding a novel zinc finger protein expressed in lymphoid cells has been isolated. This protein contains 5 repeats of the C2H2 motif previously described in the Drosophila gap gene, Kruppel, which is involved in embryo segmentation. Northern blot analysis showed that the messenger RNA (mRNA) encoding this protein is expressed at high levels in a variety of T-leukemia cell lines, at lower levels in some B cells, but is not observed in nonlymphoid cells. Within the T lineage, the mRNA is found at high levels in both alpha beta and gamma delta T cells. These data suggest that this cDNA, designated Hkr-T1, represents a gene that may contribute to the determination of the differentiation and the specificity within lymphoid cells.

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Nonspecific esterases of leukemia cell lines: evidence for activation of myeloid-associated zymogens in HL-60 by phorbol esters

Blood ◽

10.1182/blood.v63.1.238.bloodjournal631238 ◽

1984 ◽

Vol 63 (1) ◽

pp. 238-241

Author(s):

J Yourno ◽

J Walsh ◽

G Kornatowski ◽

D O'Connor ◽

SA Kumar

Keyword(s):

Cell Line ◽

Phorbol Ester ◽

Phorbol Esters ◽

De Novo ◽

Leukemia Cell ◽

Leukemia Cell Line ◽

Nonspecific Esterase ◽

Leukemia Cell Lines ◽

Myeloid Leukemia Cell ◽

Nonspecific Esterases

Myeloid leukemia cell line HL-60 contains fluoride-sensitive, myeloid- associated isoenzymes of nonspecific esterase that increase in activity when cultures are treated with phorbol ester. These isoenzymes are not detectable in B-lymphoblast cell line KLM-2, either in control or in phorbol-ester-treated cultures. No increased de novo synthesis of the isoenzymes is detectable in HL-60 treated with phorbol ester. The data suggest stimulated conversion of a preformed, myeloid-associated zymogen in HL-60.

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Nonspecific esterases of leukemia cell lines: evidence for activation of myeloid-associated zymogens in HL-60 by phorbol esters

Blood ◽

10.1182/blood.v63.1.238.238 ◽

1984 ◽

Vol 63 (1) ◽

pp. 238-241 ◽

Cited By ~ 16

Author(s):

J Yourno ◽

J Walsh ◽

G Kornatowski ◽

D O'Connor ◽

SA Kumar

Keyword(s):

Cell Line ◽

Phorbol Ester ◽

Phorbol Esters ◽

De Novo ◽

Leukemia Cell ◽

Leukemia Cell Line ◽

Nonspecific Esterase ◽

Leukemia Cell Lines ◽

Myeloid Leukemia Cell ◽

Nonspecific Esterases

Abstract Myeloid leukemia cell line HL-60 contains fluoride-sensitive, myeloid- associated isoenzymes of nonspecific esterase that increase in activity when cultures are treated with phorbol ester. These isoenzymes are not detectable in B-lymphoblast cell line KLM-2, either in control or in phorbol-ester-treated cultures. No increased de novo synthesis of the isoenzymes is detectable in HL-60 treated with phorbol ester. The data suggest stimulated conversion of a preformed, myeloid-associated zymogen in HL-60.

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Isolation of a cDNA clone encoding a zinc finger protein highly expressed in T-leukemia lines

Blood ◽

10.1182/blood.v80.10.2571.bloodjournal80102571 ◽

1992 ◽

Vol 80 (10) ◽

pp. 2571-2576

Author(s):

BY Wu ◽

EW Hanley ◽

LA Turka ◽

GJ Nabel

Keyword(s):

Zinc Finger ◽

Cdna Clone ◽

Messenger Rna ◽

Zinc Finger Protein ◽

Leukemia Cell ◽

Lymphoid Cells ◽

Gap Gene ◽

Finger Protein ◽

Leukemia Cell Lines

A cDNA clone encoding a novel zinc finger protein expressed in lymphoid cells has been isolated. This protein contains 5 repeats of the C2H2 motif previously described in the Drosophila gap gene, Kruppel, which is involved in embryo segmentation. Northern blot analysis showed that the messenger RNA (mRNA) encoding this protein is expressed at high levels in a variety of T-leukemia cell lines, at lower levels in some B cells, but is not observed in nonlymphoid cells. Within the T lineage, the mRNA is found at high levels in both alpha beta and gamma delta T cells. These data suggest that this cDNA, designated Hkr-T1, represents a gene that may contribute to the determination of the differentiation and the specificity within lymphoid cells.

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