scholarly journals Hemoglobin-spectrin complexes: interference with spectrin tetramer assembly as a mechanism for compartmentalization of band 1 and band 2 complexes

Blood ◽  
1995 ◽  
Vol 86 (1) ◽  
pp. 366-371 ◽  
Author(s):  
CR Kiefer ◽  
JF Trainor ◽  
JB McKenney ◽  
CR Valeri ◽  
LM Snyder
Keyword(s):  
Blood Cell ◽  
Western Blot ◽  
Red Blood Cell ◽  
Blot Analysis ◽  
Natural Phenomenon ◽  
Cell Aging ◽  
Cell Deformability ◽  
Self Association

The irreducible complexation of hemoglobin with spectrin is a natural phenomenon of red blood cell aging, positively correlating with increasing cell density and decreasing cell deformability. The current study begins to address the role of these complexes in the disruption of membrane skeletal physiology and structure. The effect of bound hemoglobin on spectrin dimer self-association was investigated in vitro. The extent of conversion of isolated spectrin dimers to tetramers was evaluated as a function of peroxide-induced globin complexation before the conversion incubations. The incremental accumulation of tetramer was observed to decrease with increasing peroxide concentration used in the globin complexation step. The role of oxidized heme in this process was made apparent by the inability of carboxyhemoglobin to inhibit tetramer accumulation. A Western blot analysis of naturally formed globin-spectrin conjugates demonstrated irreducible complexes of globin with both bands 1 and 2. The complexes are tentatively designated “h1” and “h2”. This analysis also demonstrated that h1 is completely extractable from cell ghosts, whereas h2 is only 50% extractable. These findings are incorporated into a hypothesis linking globin-spectrin complexation and the consequent inhibition of spectrin dimer self-association to the clustered band 3 senescence antigen (Low et al, Science 227:531, 1985).

In vitro effects of temperature on red blood cell deformability and membrane stability in human and various vertebrate species

2021 ◽  
pp. 1-10
Author(s):  
Adam Attila Matrai ◽  
Gabor Varga ◽  
Bence Tanczos ◽  
Barbara Barath ◽  
Adam Varga ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Whole Blood ◽  
Mechanical Stability ◽  
Cell Deformability ◽  
Blood Samples ◽  
Effects Of Temperature ◽  
The Way ◽  
Rbc Deformability

BACKGROUND: The effects of temperature on micro-rheological variables have not been completely revealed yet. OBJECTIVE: To investigate micro-rheological effects of heat treatment in human, rat, dog, and porcine blood samples. METHODS: Red blood cell (RBC) - buffer suspensions were prepared and immersed in a 37, 40, and 43°C heat-controlled water bath for 10 minutes. Deformability, as well as mechanical stability of RBCs were measured in ektacytometer. These tests were also examined in whole blood samples at various temperatures, gradually between 37 and 45°C in the ektacytometer. RESULTS: RBC deformability significantly worsened in the samples treated at 40 and 43°C degrees, more expressed in human, porcine, rat, and in smaller degree in canine samples. The way of heating (incubation vs. ektacytometer temperation) and the composition of the sample (RBC-PBS suspension or whole blood) resulted in the different magnitude of RBC deformability deterioration. Heating affected RBC membrane (mechanical) stability, showing controversial alterations. CONCLUSION: Significant changes occur in RBC deformability by increasing temperature, showing inter-species differences. The magnitude of alterations is depending on the way of heating and the composition of the sample. The results may contribute to better understanding the micro-rheological deterioration in hyperthermia or fever.

scholarly journals Vesiculation of Red Blood Cells in the Blood Bank: A Multi-Omics Approach towards Identification of Causes and Consequences

Proteomes ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 6
Author(s):  
Joames K. Freitas Leal ◽  
Edwin Lasonder ◽  
Vikram Sharma ◽  
Jürgen Schiller ◽  
Giuseppina Fanelli ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Blood Bank ◽  
Cell Aging ◽  
Lipid Organization ◽  
Membrane Components

Microvesicle generation is an integral part of the aging process of red blood cells in vivo and in vitro. Extensive vesiculation impairs function and survival of red blood cells after transfusion, and microvesicles contribute to transfusion reactions. The triggers and mechanisms of microvesicle generation are largely unknown. In this study, we combined morphological, immunochemical, proteomic, lipidomic, and metabolomic analyses to obtain an integrated understanding of the mechanisms underlying microvesicle generation during the storage of red blood cell concentrates. Our data indicate that changes in membrane organization, triggered by altered protein conformation, constitute the main mechanism of vesiculation, and precede changes in lipid organization. The resulting selective accumulation of membrane components in microvesicles is accompanied by the recruitment of plasma proteins involved in inflammation and coagulation. Our data may serve as a basis for further dissection of the fundamental mechanisms of red blood cell aging and vesiculation, for identifying the cause-effect relationship between blood bank storage and transfusion complications, and for assessing the role of microvesicles in pathologies affecting red blood cells.

Influence of Cyclandelate on In Vitro Red Blood Cell Deformability

Drugs ◽  
1987 ◽  
Vol 33 (Supplement 2) ◽  
pp. 30-40 ◽  
Author(s):  
D. W.R. Hall ◽  
W. E. van den Hoven
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Deformability ◽  
Red Blood Cell Deformability

The Role of Cytoskeletal S-Nitrosation in Red Blood Cell Deformability

2016 ◽  
Vol 100 ◽  
pp. S140
Author(s):  
Lukas Dominik Diederich ◽  
Thomas C. Stevenson Keller IV ◽  
Wiebke Lückstädt ◽  
Markus Dick ◽  
Malte Kelm ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Deformability ◽  
Red Blood Cell Deformability

scholarly journals Localization, Regulation and Possible Consequences of Apoptotic Protease-Activating Factor-1 (Apaf-1) Expression in Granulosa Cells of the Mouse Ovary

Endocrinology ◽  
1999 ◽  
Vol 140 (6) ◽  
pp. 2641-2644 ◽  
Author(s):  
Rodolfo Robles ◽  
Xiao-Jing Tao ◽  
Alexander M. Trbovich ◽  
Daniel V. Maravei ◽  
Ravit Nahum ◽  
...  
Keyword(s):  
Cell Death ◽  
Western Blot ◽  
Granulosa Cell ◽  
Granulosa Cells ◽  
Blot Analysis ◽  
Cell Apoptosis ◽  
Western Blot Analysis ◽  
C Elegans

Abstract The recent characterization of apoptotic protease-activating factor-1 (Apaf-1) in vertebrates as a putative homolog of the Caenorhabditis elegans gene, ced-4, indicates that the third major arm of the C. elegans programmed cell death machinery has also been conserved through evolution. Although apoptosis is now known to be important for ovarian follicular atresia in vertebrates, nothing is known of the role of Apaf-1 in ovarian function. Herein we show by immunohistochemical analysis that Apaf-1 is abundant in granulosa cells of early antral follicles whereas in vivo gonadotropin priming completely suppresses Apaf-1 expression and granulosa cell apoptosis. Western blot analysis of fractionated protein extracts prepared from granulosa cells before and after in vitro culture without hormonal support to induce apoptosis indicated that mitochondrial cytochrome c release, a biochemical step required for the activation of Apaf-1, occurs in granulosa cells cultured in vitro. Moreover, Western blot analysis of procaspase-3 processing, a principal downstream event set in motion by activated Apaf-1, indicated that healthy granulosa cells possess almost exclusively the inactive (pro-) form of the enzyme whereas granulosa cells deprived of hormonal support rapidly process procaspase-3 to the active enzyme. Lastly, we show that serum-starved granulosa cells activate caspase-3-like enzymes both prior to and after nuclear pyknosis, as revealed by a single-cell fluorescent caspase activity assay. These data, combined with previous observations regarding the role of homologs of the two other C. elegans cell death regulatory genes, ced-9 (Bc1-2 family members) and ced-3 (caspases), in atresia fully support the hypothesis that granulosa cell apoptosis is precisely coordinated by all three major arms of a cell death program conserved through evolution.

P038. The role of nitric oxide in regulation of red blood cell deformability

Nitric Oxide ◽  
2006 ◽  
Vol 14 (4) ◽  
pp. 30
Author(s):  
Xiaojun He ◽  
Ivan Azarov ◽  
Daniel B. Kim-Shapiro
Keyword(s):  
Nitric Oxide ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Deformability ◽  
Red Blood Cell Deformability

In vitro and ex vivo effects of indobufen on red blood cell deformability

1987 ◽  
Vol 32 (2) ◽  
pp. 207-210 ◽  
Author(s):  
S. Grasselli ◽  
R. Guerciolini ◽  
V. Iadevaia ◽  
P. Parise ◽  
P. Gresele ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Ex Vivo ◽  
Cell Deformability ◽  
Red Blood Cell Deformability
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