Whole genome comparative analysis of channel catfish (Ictalurus punctatus) with four model fish species

Yearling rainbow trout, Salmo gairdneri, and channel catfish, Ictalurus punctatus, were subjected to 0.5- and 3-h periods of narcosis using continuous direct current. Galvanonarcosis treatments decreased the immediate individual and group activity in both species of fish. Twenty-four hours after treatment, all fish except trout narcotized for 3 h recovered to levels of individual and group activity equivalent to those of untreated fish. Photonegative response was strong in both fish species and was not influenced by galvanonarcosis treatments. Trout narcotized with direct current were more vulnerable to predation than untreated fish; susceptability increased with increasing treatment periods. The potential of extended galvanonarcosis as a fish immobilization technique is strong. Key words: galvanonarcosis, behavior, extended narcosis, direct current, rainbow trout, channel catfish, photonegative, activity, prey vulnerability, immobilization

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Worldwide Genomic Resources for Non-Model Fish Species

Comparative and Functional Genomics ◽

10.1002/cfg.324 ◽

2003 ◽

Vol 4 (5) ◽

pp. 502-508 ◽

Cited By ~ 4

Author(s):

Melody S. Clark ◽

Douglas L. Crawford ◽

Andrew Cossins

Keyword(s):

Fish Species ◽

Genomic Resources ◽

Model Fish Species ◽

Model Fish

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Use of AMP Protein Kinase as a Potential Molecular Marker to Investigate the Development of Obesity in Non‐Model Fish Species

The FASEB Journal ◽

10.1096/fasebj.29.1_supplement.665.2 ◽

2015 ◽

Vol 29 (S1) ◽

Author(s):

Melissa Vides ◽

Paige Evans ◽

Yasuhiro Kobayashi

Keyword(s):

Protein Kinase ◽

Molecular Marker ◽

Fish Species ◽

Model Fish Species ◽

Model Fish

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Skin swabbing is a refined technique to collect DNA from model fish species

Scientific Reports ◽

10.1038/s41598-020-75304-1 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Ceinwen A. Tilley ◽

Hector Carreño Gutierrez ◽

Marion Sebire ◽

Oluwapelumi Obasaju ◽

Florian Reichmann ◽

...

Keyword(s):

Fish Species ◽

Delayed Response ◽

Alternative Procedure ◽

Fish Health ◽

Sample Collection ◽

Dna Collection ◽

Cortisol Release ◽

Refined Technique ◽

Model Fish Species ◽

Model Fish

Abstract Model fish species such as sticklebacks and zebrafish are frequently used in studies that require DNA to be collected from live animals. This is typically achieved by fin clipping, a procedure that is simple and reliable to perform but that can harm fish. An alternative procedure to sample DNA involves swabbing the skin to collect mucus and epithelial cells. Although swabbing appears to be less invasive than fin clipping, it still requires fish to be netted, held in air and handled—procedures that can cause stress. In this study we combine behavioural and physiological analyses to investigate changes in gene expression, behaviour and welfare after fin clipping and swabbing. Swabbing led to a smaller change in cortisol release and behaviour on the first day of analysis compared to fin clipping. It also led to less variability in data suggesting that fewer animals need to be measured after using this technique. However, swabbing triggered some longer term changes in zebrafish behaviour suggesting a delayed response to sample collection. Skin swabbing does not require the use of anaesthetics and triggers fewer changes in behaviour and physiology than fin clipping. It is therefore a more refined technique for DNA collection with the potential to improve fish health and welfare.

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