Dynamics of spike-and nucleocapsid specific immunity during long-term follow-up and vaccination of SARS-CoV-2 convalescents

Anti-viral immunity continuously declines over time after SARS-CoV-2 infection. Here, we characterize the dynamics of anti-viral immunity during long-term follow-up and after BNT162b2 mRNA-vaccination in convalescents after asymptomatic or mild SARS-CoV-2 infection. Virus-specific and virus-neutralizing antibody titers rapidly declined in convalescents over 9 months after infection, whereas virus-specific cytokine-producing polyfunctional T cells persisted, among which IL-2-producing T cells correlated with virus-neutralizing antibody titers. Among convalescents, 5% of individuals failed to mount long-lasting immunity after infection and showed a delayed response to vaccination compared to 1% of naïve vaccinees, but successfully responded to prime/boost vaccination. During the follow-up period, 8% of convalescents showed a selective increase in virus-neutralizing antibody titers without accompanying increased frequencies of circulating SARS-CoV-2-specific T cells. The same convalescents, however, responded to vaccination with simultaneous increase in antibody and T cell immunity revealing the strength of mRNA-vaccination to increase virus-specific immunity in convalescents.

Head-Mounted and Hand-Held Displays Diminish the Effectiveness of Fall-Resisting Skills

Use of head-mounted displays (HMDs) and hand-held displays (HHDs) may affect the effectiveness of stability control mechanisms and impair resistance to falls. This study aimed to examine whether the ability to control stability during locomotion is diminished while using HMDs and HHDs. Fourteen healthy adults (21–46 years) were assessed under single-task (no display) and dual-task (spatial 2-n-back presented on the HMD or the HHD) conditions while performing various locomotor tasks. An optical motion capture system and two force plates were used to assess locomotor stability using an inverted pendulum model. For perturbed standing, 57% of the participants were not able to maintain stability by counter-rotation actions when using either display, compared to the single-task condition. Furthermore, around 80% of participants (dual-task) compared to 50% (single-task) showed a negative margin of stability (i.e., an unstable body configuration) during recovery for perturbed walking due to a diminished ability to increase their base of support effectively. However, no evidence was found for HMDs or HHDs affecting stability during unperturbed locomotion. In conclusion, additional cognitive resources required for dual-tasking, using either display, are suggested to result in delayed response execution for perturbed standing and walking, consequently diminishing participants’ ability to use stability control mechanisms effectively and increasing the risk of falls.

Quantitative Analysis of Vegetation Dynamics in Response to Climatic Factors and Human Activities in Southwest China

We determined the time scale of normalized difference vegetation index (NDVI) response to drought and used trend and correlation analyses to explore the spatial and temporal variability characteristics of the NDVI and SPEI and their sensitivity to climatic factors in southwest China from 2000 to 2020. We used a partial derivative approach to calculate the contributions of six climatic factors and human activities to the interannual variation in the NDVI. The results demonstrated that from 2000 to 2020, the annual mean NDVI in southwest China showed a slight decreasing trend at a rate of 0.0001 y−1. The NDVI had the highest sensitivity to the standardized precipitation and evapotranspiration index on a 12-month time scale. The NDVI exhibited a 1-year delayed response to drought. The SPEI has the highest sensitivity to precipitation. The percentage of pixels with a positive correlation between NDVI and precipitation, mean temperature, temperature difference, mean relative humidity, mean wind speed, and sunshine duration in the study area was 31.73%, 46.81%, 35.49%, 25.76%, 39.36%, and 39.89%, respectively. The average contributions of these six climatic factors to the interannual variation of NDVI were 0.00029, 0.00046, −0.00007, 0.00007, 0.0008, and 0.00001 y−1, respectively. The NDVI had the highest sensitivity to mean temperature and the lowest sensitivity to mean relative humidity. The average contributions of climatic factors and human activities to interannual variability in southwest China were 0.00156 and 0.00012 y−1, respectively. The positive influence of climatic factors on the NDVI was stronger than that of human activities. This study provides a theoretical basis for the sustainable management of the regional ecological environment.

Effect of bilingualism on infants’ cognitive flexibility

Research suggests that bilinguals often outperform monolinguals on tasks that tap into executive functions, such as those requiring conflict resolution and cognitive flexibility. Recently, better attentional control has been detected in infants as young as 6 months, thereby providing a possible basis for a cognitive benefit before language production. The goal of the present study was to examine if cognitive flexibility is more advanced in bilingual infants. A detour reaching task assessing conflict resolution, a delayed response task assessing shifting, and a multiple location task assessing maintaining, were administered to 17-month-old infants. The main findings revealed that being bilingual did not improve performance on any of the executive function tasks. Furthermore, current exposure to a second language or language proficiency did not impact executive functioning. We conclude that a bilingual advantage in cognitive flexibility may not be present before children have enough experience in code switching.

The evolution of primate short-term memory

Short-term memory is implicated in a range of cognitive abilities and is critical for understanding primate cognitive evolution. To investigate the effects of phylogeny, ecology and sociality on short-term memory ability, we tested 421 non-human primates across 41 species in a pre-registered, experimental delayed-response task. Our results confirm previous findings that longer delays decrease memory performance across species and taxa. Our analyses demonstrate a considerable contribution of phylogeny over ecological and social factors on the distribution of short-term memory performance in primates; closely related species had more similar short-term memory abilities. However, interdependencies between phylogeny and socioecology of a given species present an obstacle to disentangling the effects of each of these factors on the evolution of short-term memory capacity. The dataset corresponding to the study is freely accessible and constitutes an important resource for studying the evolution of primate cognition.

Environmental and anthropogenic gravity contributions at the Þeistareykir geothermal field, North Iceland

Continuous high-resolution gravimetry is increasingly used to monitor mass distribution changes in volcanic, hydrothermal or other complex geosystems. To quantify the often small target signals, gravity contributions from, e.g. atmospheric mass changes, global and local hydrology should be accounted for. We set up three iGrav superconducting gravity meters for continuous monitoring of the Þeistareykir geothermal field in North Island. Additionally, we installed a set of hydrometeorological sensors at each station for continuous observation of local pressure changes, soil moisture, snow and vertical surface displacement. We show that the contribution of these environmental parameters to the gravity signal does not exceed 10 µGal (1 µGal = 10–8 m s−2), mainly resulting from vertical displacement and snow accumulation. The seasonal gravity contributions (global atmosphere, local and global hydrology) are in the order of ± 2 µGal at each station. Using the environmental observations together with standard gravity corrections for instrumental drift and tidal effects, we comprehensively reduced the iGrav time-series. The gravity residuals were compared to groundwater level changes and geothermal mass flow rates (extraction and injection) of the Þeistareykir power plant. The direct response of the groundwater levels and a time-delayed response of the gravity signal to changes in extraction and injection suggest that the geothermal system is subject to a partially confined aquifer. Our observations indicate that a sustainable “equilibrium” state of the reservoir is reached at extraction flow rates below 240 kg s−1 and injection flow rates below 160 kg s−1. For a first-order approximation of the gravity contributions from extracted and injected masses, we applied a simplified forward gravity model. Comparison to the observed gravity signals suggest that most of the reinjected fluid is drained off through the nearby fracture system.

Operating Effects of the Three Gorges Reservoir on the Riverbed Stability in the Wuhan Reach of the Yangtze River

Reservoir construction may modify the downstream flow and sediment transport, and correspondingly result in adjustments in morphodynamics of a river, especially riverbed instability. Based on hydrological datasets from 2003 to 2019 during the post-dam period using the topographic data of 57 fixed cross-sections in the Wuhan reach of the Yangtze River, we calculated the indexes representing the channel stability. Moreover, considering the effects of flow, sediment concentration, grain size of sediment, and water depth, we propose a method for calculating the equilibrium values of cumulative erosion and the lateral migrate intensity of thalweg in this paper, and the method combines with the delayed response model (DRM) to comprehensively analyze the variations in the longitudinal and lateral stabilities of the riverbed. The results revealed that the channel has been obviously eroded in the downstream reach, resulting in a 76% decrease in sediment discharge after the impoundment of the Three Gorges Reservoir (TGR). Specifically, in the past 17 years, the cumulative erosion in the Wuhan reach of the Yangtze River reached 1.72 m, while the bankfull depth increased by 1.87 m. The lateral migrate intensity of thalweg increased in response to Coriolis force, with an increase of 22.3%. Taken together, the results show that the proposed formula can effectively simulate the variation process of channel stability, and it also quantifies the extent of the influence weight of interannual flow and sediment regimes. The morphodynamics adjustments in the channel stability of our studied reach were closely related to the previous five-year flow and sediment regimes, implying that channel evolution may lag behind the changes in flow and sediment discharges.

An Effective Communication Prototype for Time-Critical IIoT Manufacturing Factories Using Zero-Loss Redundancy Protocols, Time-Sensitive Networking, and Edge-Computing in an Industry 4.0 Environment

The Industrial Internet of things (IIoT), the implementation of IoT in the industrial sector, requires a deterministic, real-time, and low-latency communication response for its time-critical applications. A delayed response in such applications could be life-threatening or result in significant losses for manufacturing plants. Although several measures in the likes of predictive maintenance are being put in place to prevent errors and guarantee high network availability, unforeseen failures of physical components are almost inevitable. Our research contribution is to design an efficient communication prototype, entirely based on internet protocol (IP) that combines state-of-the-art communication computing technologies principles to deliver a more stable industrial communication network. We use time-sensitive networking (TSN) and edge computing to increase the determinism of IIoT networks, and we reduce latency with zero-loss redundancy protocols that ensure the sustainability of IIoT networks with smooth recovery in case of unplanned outages. Combining these technologies altogether brings more effectiveness to communication networks than implementing standalone systems. Our study results develop two experimental IP-based industrial network communication prototypes in an intra-domain transmission scenario: the first one is based on the parallel zero-loss redundancy protocol (PRP) and the second one using the high-availability seamless zero-loss redundancy protocol (HSR). We also highlight the benefits of utilizing our communication prototypes to build robust industrial IP communication networks with high network availability and low latency as opposed to conventional communication networks running on seldom redundancy protocols such as Media Redundancy Protocol (MRP) or Rapid Spanning Tree Protocol (RSTP) with single-point of failure and delayed recovery time. While our two network communication prototypes—HSR and PRP—offer zero-loss recovery time in case of a single network failure, our PRP communication prototype goes a step further by providing an effective redundancy scheme against multiple link failures.

Picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera

Background Gerbera jamesonii Bolus ex Hooker f. (African daisy) is listed among the top five most important ornamental plants in the global floricultural industry. To satisfy its demand, the floriculture industry relies on reproducible and effective propagation protocol while retaining the genetic uniformity of G. jamesonii. The present study, for the first time, reports the potential of picloram for enhanced induction of organogenic calli from leaves of G. jamesonii and its high-frequency indirect regeneration. Results The fastest induction of calli with maximum fresh and dry weight was recorded in the Murashige and Skoog (MS) semisolid medium supplemented with 1 mg/l picloram. In addition, callus induction was observed in 2,4-dichlorophenoxy acetic acid- and α-napthaleneaceticacid-supplemented media but with delayed response and reduced fresh and dry weight. The proliferated calli were transferred to shoot induction media containing MS salt and 0.5–1 mg/l N6-benzylaminopurine, kinetin, or thidiazuron. A mean number of ~6 shoots per callus were developed after 5 days of culture in the MS medium supplemented with 1 mg/l kinetin, with a mean length of 5.2 cm. Successful rooting of shoots was achieved in the MS medium fortified with 1.5 mg/l indole-3-acetic acid, wherein the earliest root initiation (~5 days), as well as the maximum number (~9) and length (~4.8 cm) of roots, were recorded. Complete plantlets were primarily acclimatized in sand before being transferred to a mixed substrate (of soil, sand, tea leaf waste, and cow urine) that secured >90% survival and further growth of the plantlets. Eventually, clonal fidelity of the in vitro regenerants assessed via inter-simple sequence repeats (ISSR) primers exhibited a monomorphic banding patterns that suggested genetic integrity within the plantlets as well as with their mother plant. Conclusions The results of the present study should be of interest for commercial propagation and mutagenesis- as well as genetic transformation-related research.