scholarly journals Analysis of Nanoarchaeum equitans genome and proteome composition: indications for hyperthermophilic and parasitic adaptation

BMC Genomics ◽  
2006 ◽  
Vol 7 (1) ◽  
Author(s):  
Sabyasachi Das ◽  
Sandip Paul ◽  
Sumit K Bag ◽  
Chitra Dutta
Keyword(s):  
Nanoarchaeum Equitans ◽  
Parasitic Adaptation

scholarly journals Nanoarchaeal Origin of Histone H3?

2008 ◽  
Vol 191 (3) ◽  
pp. 1092-1096 ◽  
Author(s):  
Ulrike Friedrich-Jahn ◽  
Johanna Aigner ◽  
Gernot Längst ◽  
John N. Reeve ◽  
Harald Huber
Keyword(s):  
Histone H3 ◽  
Evolutionary Step ◽  
Nanoarchaeum Equitans ◽  
Archaeal Histone

ABSTRACT NEQ288, one of two archaeal histones in Nanoarchaeum equitans, has a unique four-residue insertion that closely resembles an insertion in the eukaryotic histone H3 lineage. NEQ288 bound DNA but did not compact DNA in vitro in the absence of NEQ348, the second N. equitans archaeal histone. The properties of NEQ288 suggest an intermediate between the archaeal and H3 histone lineages and an evolutionary step toward the now-mandatory assembly of eukaryotic histones into heterodimers.

scholarly journals Proteomic Characterization of Cellular and Molecular Processes that Enable the Nanoarchaeum equitans-Ignicoccus hospitalis Relationship

PLoS ONE ◽  
2011 ◽  
Vol 6 (8) ◽  
pp. e22942 ◽  
Author(s):  
Richard J. Giannone ◽  
Harald Huber ◽  
Tatiana Karpinets ◽  
Thomas Heimerl ◽  
Ulf Küper ◽  
...  
Keyword(s):  
Molecular Processes ◽  
Ignicoccus Hospitalis ◽  
Nanoarchaeum Equitans

scholarly journals Ignicoccus hospitalis sp. nov., the host of ‘Nanoarchaeum equitans’

2007 ◽  
Vol 57 (4) ◽  
pp. 803-808 ◽  
Author(s):  
Walter Paper ◽  
Ulrike Jahn ◽  
Michael J. Hohn ◽  
Michaela Kronner ◽  
Daniela J. Näther ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Protein Composition ◽  
High Similarity ◽  
The North ◽  
Physiological Properties ◽  
Cell Architecture ◽  
Ignicoccus Hospitalis ◽  
Nanoarchaeum Equitans ◽  
Unique Cell

A novel chemolithoautotrophic and hyperthermophilic member of the genus Ignicoccus was isolated from a submarine hydrothermal system at the Kolbeinsey Ridge, to the north of Iceland. The new isolate showed high similarity to the two species described to date, Ignicoccus islandicus and Ignicoccus pacificus, in its physiological properties as well as in its unique cell architecture. However, phylogenetic analysis and investigations on the protein composition of the outer membrane demonstrated that the new isolate was clearly distinct from I. islandicus and I. pacificus. Furthermore, it is the only organism known so far which is able to serve as a host for ‘Nanoarchaeum equitans’, the only cultivated member of the ‘Nanoarchaeota’. Therefore, the new isolate represents a novel species of the genus Ignicoccus, which we name Ignicoccus hospitalis sp. nov. (type strain KIN4/IT=DSM 18386T=JCM 14125T).

Comparative transcriptomics of two pathogenic pinewood nematodes yields insights into parasitic adaptation to life on pine hosts

Gene ◽  
2012 ◽  
Vol 505 (1) ◽  
pp. 81-90 ◽  
Author(s):  
Xia Yan ◽  
Xin-Yue Cheng ◽  
Yun-Sheng Wang ◽  
Ji Luo ◽  
Zhen-Chuan Mao ◽  
...  
Keyword(s):  
Comparative Transcriptomics ◽  
Pinewood Nematodes ◽  
Parasitic Adaptation

scholarly journals Transcribing Genes the Hard Way: In Vitro Reconstitution of Nanoarchaeal RNA Polymerase Reveals Unusual Active Site Properties

2021 ◽  
Vol 8 ◽  
Author(s):  
Sven Nottebaum ◽  
Robert O. J. Weinzierl
Keyword(s):  
Rna Polymerase ◽  
High Throughput Screening ◽  
Sparse Matrix ◽  
Screening Method ◽  
Fluoride Ions ◽  
Stringent Requirement ◽  
In Vitro Reconstitution ◽  
Nanoarchaeum Equitans ◽  
Complete Set

Nanoarchaea represent a highly diverged archaeal phylum that displays many unusual biological features. The Nanoarchaeum equitans genome encodes a complete set of RNA polymerase (RNAP) subunits and basal factors. Several of the standard motifs in the active center contain radical substitutions that are normally expected to render the polymerase catalytically inactive. Here we show that, despite these unusual features, a RNAP reconstituted from recombinant Nanoarchaeum subunits is transcriptionally active. Using a sparse-matrix high-throughput screening method we identified an atypical stringent requirement for fluoride ions to maximize its activity under in vitro transcription conditions.

The interaction of Nanoarchaeum equitans with Ignicoccus hospitalis: proteins in the contact site between two cells

2009 ◽  
Vol 37 (1) ◽  
pp. 127-132 ◽  
Author(s):  
Tillmann Burghardt ◽  
Benjamin Junglas ◽  
Frank Siedler ◽  
Reinhard Wirth ◽  
Harald Huber ◽  
...  
Keyword(s):  
Protein Complex ◽  
Direct Contact ◽  
Cell Surfaces ◽  
Contact Site ◽  
Electron Microscopic ◽  
Intimate Association ◽  
Ignicoccus Hospitalis ◽  
Nanoarchaeum Equitans ◽  
Cell Envelopes ◽  
Microscopic Investigations

The two archaea Ignicoccus hospitalis and Nanoarchaeum equitans form a unique intimate association, the character of which is not yet fully understood. Electron microscopic investigations show that at least two modes of cell–cell interactions exist: (i) the two cells are interconnected via thin fibres; and (ii) the two cell surfaces are in direct contact with each other. In order to shed further light on the molecules involved, we isolated a protein complex, by using detergent-induced solubilization of cell envelopes, followed by a combination of chromatography steps. Analysis by MS and comparison with databases revealed that this fraction contained two dominant proteins, representing the respective major envelope proteins of the two archaea. In addition, a considerable set of membrane proteins is specifically associated with these proteins. They are now the focus of further biochemical and ultrastructural investigations.

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