scholarly journals A two-genome microarray for the rice pathogens Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola and its use in the discovery of a difference in their regulation of hrp genes

2008 ◽  
Vol 8 (1) ◽  
pp. 99 ◽  
Author(s):  
Young-Su Seo ◽  
Malinee Sriariyanun ◽  
Li Wang ◽  
Janice Pfeiff ◽  
Jirapa Phetsom ◽  
...  
Keyword(s):  
Xanthomonas Oryzae ◽  
Hrp Genes ◽  
Rice Pathogens

scholarly journals Concomitant Regulation by a LacI-Type Transcriptional Repressor XylR on Genes Involved in Xylan and Xylose Metabolism and the Type III Secretion System in Rice Pathogen Xanthomonas oryzae pv. oryzae

2018 ◽  
Vol 31 (6) ◽  
pp. 605-613 ◽  
Author(s):  
Yumi Ikawa ◽  
Sayaka Ohnishi ◽  
Akiko Shoji ◽  
Ayako Furutani ◽  
Seiji Tsuge
Keyword(s):  
Gene Expression ◽  
Type Iii Secretion ◽  
Type Iii Secretion System ◽  
Xylose Isomerase ◽  
Secretion System ◽  
Xanthomonas Oryzae ◽  
Upstream Region ◽  
Xylose Metabolism ◽  
Type Iii ◽  
Hrp Genes

The hypersensitive response and pathogenicity (hrp) genes of Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight of rice, encode components of the type III secretion system and are essential for virulence. Expression of hrp genes is regulated by two key hrp regulators, HrpG and HrpX; HrpG regulates hrpX and hrpA, and HrpX regulates the other hrp genes on hrpB-hrpF operons. We previously reported the sugar-dependent quantitative regulation of HrpX; the regulator highly accumulates in the presence of xylose, followed by high hrp gene expression. Here, we found that, in a mutant lacking the LacI-type transcriptional regulator XylR, HrpX accumulation and hrp gene expression were high even in the medium without xylose, reaching the similar levels present in the wild type incubated in the xylose-containing medium. XylR also negatively regulated one of two xylose isomerase genes (xylA2 but not xylA1) by binding to the motif sequence in the upstream region of the gene. Xylose isomerase is an essential enzyme in xylose metabolism and interconverts between xylose and xylulose. Our results suggest that, in the presence of xylose, inactivation of XylR leads to greater xylan and xylose utilization and, simultaneously, to higher accumulation of HrpX, followed by higher hrp gene expression in the bacterium.

scholarly journals An H-NS-like protein involved in the negative regulation of hrp genes in Xanthomonas oryzae pv. oryzae

2011 ◽  
Vol 319 (1) ◽  
pp. 58-64 ◽  
Author(s):  
Yumi Kametani-Ikawa ◽  
Seiji Tsuge ◽  
Ayako Furutani ◽  
Hirokazu Ochiai
Keyword(s):  
Negative Regulation ◽  
Xanthomonas Oryzae ◽  
Hrp Genes

Expression of Xanthomonas oryzae pv. oryzae hrp Genes in XOM2, a Novel Synthetic Medium

2002 ◽  
Vol 68 (4) ◽  
pp. 363-371 ◽  
Author(s):  
Seiji TSUGE ◽  
Ayako FURUTANI ◽  
Rie FUKUNAKA ◽  
Takashi OKU ◽  
Kazunori TSUNO ◽  
...  
Keyword(s):  
Synthetic Medium ◽  
Xanthomonas Oryzae ◽  
Hrp Genes

A leucine-rich protein, LrpX, is a new regulator of hrp genes in Xanthomonas oryzae pv. oryzae

2008 ◽  
Vol 75 (1) ◽  
pp. 66-71 ◽  
Author(s):  
Md. Rashidul Islam ◽  
Md. Shahinur Kabir ◽  
Hisae Hirata ◽  
Seiji Tsuge ◽  
Shinji Tsuyumu
Keyword(s):  
Xanthomonas Oryzae ◽  
Hrp Genes

scholarly journals Involvement of Phosphoglucose Isomerase in Pathogenicity of Xanthomonas oryzae pv. oryzae

2004 ◽  
Vol 94 (5) ◽  
pp. 478-483 ◽  
Author(s):  
Seiji Tsuge ◽  
Hirokazu Ochiai ◽  
Yasuhiro Inoue ◽  
Takashi Oku ◽  
Kazunori Tsuno ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Hypersensitive Response ◽  
Synthetic Medium ◽  
Transposon Mutagenesis ◽  
Leaf Blight ◽  
Phosphoglucose Isomerase ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Carbohydrate Source ◽  
Hrp Genes

Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight of rice, was subjected to transposon mutagenesis to generate mutants defective in pathogenicity. A novel mutant 74M913 was attenuated in virulence but retained its ability to cause the hypersensitive response in leaf blight-resistant rice and tomato. Cloning and sequence analysis revealed that the transposon in 74M913 was inserted in a gene homologous to the phosphoglucose isomerase (pgi) gene of X. axonopodis pv. citri. Growth of the mutant in a synthetic medium containing fructose or xylose as a sole carbohydrate source was much reduced, indicating the transposon disrupted pgi function. The interaction between expression of pgi and hypersensitive response and pathogenicity (hrp) genes was investigated because we had demonstrated previously that expression of hrp genes of X. oryzae pv. oryzae is induced in a synthetic medium containing xylose. However, pgi and the hrp gene (hrcU) were expressed independently. This study suggests that PGI is involved in pathogenicity of X. oryzae pv. oryzae.

scholarly journals A Novel Regulatory Role of HrpD6 in Regulating hrp-hrc-hpa Genes in Xanthomonas oryzae pv. oryzicola

2011 ◽  
Vol 24 (9) ◽  
pp. 1086-1101 ◽  
Author(s):  
Yu-Rong Li ◽  
Hua-Song Zou ◽  
Yi-Zhou Che ◽  
Yi-Ping Cui ◽  
Wei Guo ◽  
...  
Keyword(s):  
Hypersensitive Response ◽  
Type Iii Secretion ◽  
Xanthomonas Oryzae ◽  
Host Cells ◽  
Bacterial Leaf Streak ◽  
Two Component System ◽  
Tal Effectors ◽  
Master Regulators ◽  
Hrp Genes

Xanthomonas oryzae pv. oryzicola, the causal agent of bacterial leaf streak in the model plant rice, possesses a hypersensitive response and pathogenicity (hrp), hrp-conserved (hrc), hrp-associated (hpa) cluster (hrp-hrc-hpa) that encodes a type III secretion system (T3SS) through which T3SS effectors are injected into host cells to cause disease or trigger plant defenses. Mutations in this cluster usually abolish the bacterial ability to cause hypersensitive response in nonhost tobacco and pathogenicity in host rice. In Xanthomonas spp., these genes are generally assumed to be regulated by the key master regulators HrpG and HrpX. However, we present evidence that, apart from HrpG and HrpX, HrpD6 is also involved in regulating the expression of hrp genes. Interestingly, the expression of hpa2, hpa1, hpaB, hrcC, and hrcT is positively controlled by HrpD6. Transcriptional expression assays demonstrated that the expression of the hrcC, hrpD5, hrpE, and hpa3 genes was not completely abolished by hrpG and hrpX mutations. As observed in analysis of their corresponding mutants, HrpG and HrpX exhibit contrasting gene regulation, particularly for hpa2 and hrcT. Other two-component system regulators (Zur, LrpX, ColR/S, and Trh) did not completely inhibit the expression of hrcC, hrpD5, hrpE, and hpa3. Immunoblotting assays showed that the secretion of HrpF, which is an HpaB-independent translocator, is not affected by the mutation in hrpD6. However, the mutation in hrpD6 affects the secretion of an HpaB-dependent TAL effector, AvrXa27. These novel findings suggest that, apart from HrpG and HrpX, HrpD6 plays important roles not only in the regulation of hrp genes but also in the secretion of TAL effectors.

Identification and Molecular Mapping of Xa32(t), a Novel Resistance Gene for Bacterial Blight (Xanthomonas oryzae pv. oryzae) in Rice

2009 ◽  
Vol 35 (7) ◽  
pp. 1173-1180 ◽  
Author(s):  
Chong-Ke ZHENG ◽  
Chun-Lian WANG ◽  
Yuan-Jie YU ◽  
Yun-Tao LIANG ◽  
Kai-Jun ZHAO
Keyword(s):  
Resistance Gene ◽  
Bacterial Blight ◽  
Molecular Mapping ◽  
Xanthomonas Oryzae

Changes in macromolecular synthesis in Xanthomonas oryzae infected with bacteriophage XP-12.

1977 ◽  
Vol 23 (3) ◽  
pp. 517-523 ◽  
Author(s):  
M Ehrlich ◽  
F H Lin ◽  
K Ehrlich ◽  
S L Brown ◽  
J A Mayo
Keyword(s):  
Xanthomonas Oryzae ◽  
Macromolecular Synthesis
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