scholarly journals Tian Xian Liquid (TXL) induces apoptosis in HT-29 colon cancer cell in vitro and inhibits tumor growth in vivo

2010 ◽  
Vol 5 (1) ◽  
pp. 25 ◽  
Author(s):  
Qing Liu ◽  
Yao Tong ◽  
Stephen Sze ◽  
Wing Liu ◽  
Lam Lam ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Tumor Growth ◽  
Cancer Cell ◽  
Colon Cancer Cell ◽  
Ht 29

scholarly journals In vitro and in vivo evaluation of colon cancer targeted epichlorohydrin crosslinked Portulaca-alginate beads

2018 ◽  
Vol 9 (1) ◽  
pp. 190-199 ◽  
Author(s):  
Geet P. Asnani ◽  
Chandrakant R. Kokare
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
In Vivo Studies ◽  
Ht 29

AbstractThe aim of this study was to formulate a novel dual crosslinked hydrogel bead using Portulaca mucilage for colon-targeted delivery of 5-fluorouracil (5-FU) and evaluate its safety, specificity and efficacy. The ionotropic gelation technique was employed to prepare the hydrogel beads of Portulaca mucilage. For this, the mucilage was initially crosslinked with alginate and calcium ions. Epichlorohydrin was employed as a crosslinker in the second crosslinking step. The formulation was subjected to in vitro and in vivo studies to evaluate morphology, size, cytotoxicity, and organ distribution. Human HT-29 colon cancer cell-line was used for in vitro assays and in vivo studies were performed in Wistar rats to assess the usefulness and effectiveness of the formulation for colon cancer therapy. Microsphere sizes ranged from 930 to 977μm and possessed a high level of drug encapsulation efficiency (ca. 78% w/w). Compared with 5-FU solution (Tmax = 1.2 h, mean resident time: MRT = 3.3h) the dual crosslinked Portulaca microspheres exhibited sustained drug release after oral administration to rats (Tmax = 16h, MRT = 14h). The relative bioavailability of 5-FU solution and the microspheres were 100 and 93.6% respectively. Tissue distribution studies indicated high concentration of 5-FU in colon. In-vitro anticancer assay demonstrated IC50 value of 11.50 μg/ml against HT-29 colon cancer cell line. The epichlorohydrin cross-linked Portulaca microspheres prepared in this study provided sustained release of 5-FU up to 16h in the colonic region and enhanced the antitumor activity of the neoplastic drug. The formulation is hence an ideal carrier system for colon-targeted drug delivery.

scholarly journals Alpha‐mangostin reduces HT‐29 colon cancer cell proliferation in vitro and inhibits transplantable tumorigenesis in vivo.

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Chureeporn Chitchumroonchokchai ◽  
Sunit Suksumrarn ◽  
Jun‐ge Yu ◽  
Steven K. Clinton ◽  
Mark L. Failla
Keyword(s):  
Colon Cancer ◽  
Cell Proliferation ◽  
Cancer Cell ◽  
Colon Cancer Cell ◽  
Cancer Cell Proliferation ◽  
Proliferation In Vitro ◽  
Ht 29

scholarly journals A critical role of CCR7 in invasiveness and metastasis of SW620 colon cancer cell in vitro and in vivo

2008 ◽  
Vol 7 (7) ◽  
pp. 1037-1043 ◽  
Author(s):  
Yu Shuyi ◽  
Duan Juping ◽  
Zhou Zhiqun ◽  
Pang Qiong ◽  
Ji Wuyang ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cell ◽  
Critical Role ◽  
Colon Cancer Cell

GREEN SYNTHESIS OF GOLD NANOPARTICLES OF ISOLATED CITRUS BIOFLAVONOID FROM ORANGE: CHARACTERIZATION AND IN VITRO CYTOTOXICITY AGAINST COLON CANCER CELL LINES COLO 320DM AND HT29

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (08) ◽  
pp. 61-69
Author(s):  
D. S Randive ◽  
K. P. Shejawal ◽  
S. D Bhinge ◽  
M. A Bhutkar ◽  
P. D. Patil ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Gold Nanoparticles ◽  
Green Synthesis ◽  
Cancer Cell ◽  
Orange Peel ◽  
In Vitro Cytotoxicity ◽  
Colon Cancer Cell ◽  
Cytotoxicity Studies ◽  
Ht 29

The aim of the present research was to perform green synthesis of gold nanoparticles of isolated citrus bioflavonoid from Citrus sinensis (orange) peel extract and to evaluate its anticancer potential. Methanolic extract of orange peel was obtained by Soxhlet extraction and citrus bioflavonoid was isolated by using column chromatography. Gold nanoparticles were developed by green synthesis method, wherein 1 % aqueous solution of isolated citrus bioflavonoid were mixed with 1% solution of HAuCl4 and incubated at ambient temperature for 4 to 5 hours and observed for the color change which indicated formation of nanoparticles. Obtained gold nanoparticles were evaluated by UV visible spectra, FTIR, SEM, XRD analysis and for antimicrobial potential against E coli, S.aureus and P. aeruginosa. Cytotoxicity study was carried out by using in vitro assays, namely MTT, SRB and Tryphan blue assay, against colon cancer cell line Colo 320 DM, and HT 29. results of SEM showed that nanosized particles in the range of 80-100nm were formed. Results of cytotoxicity studies revealed that CBFGNP exhibited 72.28% inhibition, against Colo320 DM whereas pure CBF showed 70.46% inhibition. Against HT 29, CBFGNP exhibited 69.79% inhibition, whereas for MTT assay and SRB assay, CBFGNP showed 80.15% and 58.29% inhibition, respectively. Moreover, CBFGNP exhibited 90.29% and 85% non viability against Colo320 DM and HT29. Based on the results, it can be concluded that gold nanoparticles of citrus bioflavonoid (CBFGNP) exhibits more cytotoxicity against Colo320 DM and HT29 as compared to pure citrus bioflavonoid when assessed by three different in vitro cytotoxicity assays.

The histone deacetylase inhibitor PXD101 synergises with 5-fluorouracil to inhibit colon cancer cell growth in vitro and in vivo

2006 ◽  
Vol 60 (2) ◽  
pp. 275-283 ◽  
Author(s):  
Anthony Tumber ◽  
Laura S. Collins ◽  
Kamille Dumong Petersen ◽  
Annemette Thougaard ◽  
Sanne J. Christiansen ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Growth ◽  
Histone Deacetylase ◽  
Cancer Cell ◽  
Histone Deacetylase Inhibitor ◽  
Colon Cancer Cell ◽  
Cancer Cell Growth ◽  
Deacetylase Inhibitor
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