In vitro anthelmintic activity of Pothomorphe umbellata (L.) Miq. (Piperaceae) against gastrointestinal parasites from sheep

Phytotherapy may be an alternative strategy for controlling gastrointestinal parasites. This study evaluated the anthelmintic efficacy of Eucalyptus citriodora essential oil (EcEO). The in vitro effects of EcEO were determined through testing the inhibition of egg hatching and larval development of Haemonchus contortus. EcEO was subjected to acute toxicity testing on mice, orally and intraperitoneally. The in vivo effects of EcEO were determined by the fecal egg count reduction test (FECRT) in goats infected with gastrointestinal nematodes. The results showed that 5.3 mg.mL-1 EcEO inhibited egg hatching by 98.8% and 10.6 mg.mL-1 EcEO inhibited H. contortus larval development by 99.71%. The lethal doses for 50% of the mice were 4153 and 622.8 mg.kg-1, for acute toxicity orally and intraperitoneally. In the FECRT, the efficacy of EcEO and ivermectin was 66.25 and 79.16% respectively, on goat gastrointestinal nematodes eight days after treatment. EcEO showed in vitro and in vivo anthelmintic activity.

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Prosopis juliflora pods alkaloid-rich fraction: In vitro anthelmintic activity on goat gastrointestinal parasites and its cytotoxicity on vero cells

Pharmacognosy Magazine ◽

10.4103/pm.pm_3_17 ◽

2017 ◽

Vol 13 (51) ◽

pp. 684 ◽

Cited By ~ 3

Author(s):

Alexsandro Branco ◽

HelimarGonçalves Lima ◽

DaniloCavalcante Gomes ◽

NatháliaSilva Santos ◽

ÊuderReis Dias ◽

...

Keyword(s):

Anthelmintic Activity ◽

Vero Cells ◽

Prosopis Juliflora ◽

Gastrointestinal Parasites

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In vitro effects of Coriandrum sativum, Tagetes minuta, Alpinia zerumbet and Lantana camara essential oils on Haemonchus contortus

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612013000400004 ◽

2013 ◽

Vol 22 (4) ◽

pp. 463-469 ◽

Cited By ~ 10

Author(s):

Iara Tersia Freitas Macedo ◽

Lorena Mayana Beserra de Oliveira ◽

Ana Lourdes Fernandes Camurça-Vasconcelos ◽

Wesley Lyeverton Correia Ribeiro ◽

Jessica Maria Leite dos Santos ◽

...

Keyword(s):

Essential Oils ◽

Larval Development ◽

Haemonchus Contortus ◽

Small Ruminants ◽

Anthelmintic Activity ◽

Gastrointestinal Parasites ◽

Coriandrum Sativum ◽

Tagetes Minuta ◽

Alpinia Zerumbet

Phytotherapy can be an alternative for the control of gastrointestinal parasites of small ruminants. This study evaluated the efficacy of Alpinia zerumbet, Coriandrum sativum, Tagetes minuta and Lantana camaraessential oils by two in vitro assays on Haemonchus contortus, an egg hatch test (EHT) and larval development test (LDT). No effect was observed for L. camara in the EHT. A. zerumbet, C. sativum and T. minuta essential oils exhibited a dose-dependent effect in the EHT, inhibiting 81.2, 99 and 98.1% of H. contortus larvae hatching, respectively, at a concentration of 2.5 mg mL-1. The effective concentration to inhibit 50% (EC50) of egg hatching was 0.94, 0.63 and 0.53 mg mL-1 for A. zerumbet, C. sativum and T. minuta essential oils, respectively. In LDT, L. camara, A. zerumbet, C. sativum and T. minuta at concentration of 10 mg mL-1 inhibited 54.9, 94.2, 97.8 and 99.5% of H. contortus larval development, presenting EC50 values of 6.32, 3.88, 2.89 and 1.67 mg mL-1, respectively. Based on the promising results presented in this in vitro model, it may be possible use of these essential oils to control gastrointestinal nematodes. However, their anthelmintic activity should be confirmed in vivo.

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Hydrolyzable tannins fighting gastrointestinal parasites of ruminants: chemical structure predicts in vitro anthelmintic activity

Planta Medica ◽

10.1055/s-0036-1596980 ◽

2016 ◽

Vol 81 (S 01) ◽

pp. S1-S381

Author(s):

MT Engström ◽

M Karonen ◽

JR Ahern ◽

N Baert ◽

B Payré ◽

...

Keyword(s):

Chemical Structure ◽

Anthelmintic Activity ◽

Gastrointestinal Parasites ◽

Hydrolyzable Tannins

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In vitro anthelmintic activity of Pimienta dioica and Origanum vulgare essential oils on gastrointestinal nematodes from sheep and cattle

Journal of Parasitic Diseases ◽

10.1007/s12639-021-01401-1 ◽

2021 ◽

Author(s):

Gerardo Jiménez-Penago ◽

Roberto González-Garduño ◽

Luciano Martínez-Bolaños ◽

Ema Maldonado-Siman ◽

Alvar A. Cruz-Tamayo ◽

...

Keyword(s):

Essential Oils ◽

Anthelmintic Activity ◽

Gastrointestinal Nematodes ◽

Origanum Vulgare

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Chemical characterization and in vitro anthelmintic activity of Citrus bergamia Risso and Citrus X paradisii Macfad essential oil against Haemonchus contortus Kirby isolate

Acta Tropica ◽

10.1016/j.actatropica.2021.105869 ◽

2021 ◽

Vol 217 ◽

pp. 105869

Author(s):

Vivien Patricia Garbin ◽

Beatriz Munguía ◽

Jenny Carolina Saldaña ◽

Cícero Deschamps ◽

Roger Raupp Cipriano ◽

...

Keyword(s):

Essential Oil ◽

Haemonchus Contortus ◽

Chemical Characterization ◽

Anthelmintic Activity ◽

Citrus Bergamia

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In vitro anthelmintic activity of Dennettia tripetala G. Baker (Annonaceae) Fruits against Haemonchus contortus

Journal of Parasitic Diseases ◽

10.1007/s12639-021-01438-2 ◽

2021 ◽

Author(s):

Rachel A. Nwosu ◽

Mohammed M. Suleiman ◽

Hussaina J. Makun ◽

Matthew P. Ameh ◽

Manji A. Shetshak ◽

...

Keyword(s):

Haemonchus Contortus ◽

Anthelmintic Activity ◽

Dennettia Tripetala

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In Vitro Nematocidal Effect and Anthelmintic Activity of Artemisia cina Against Haemonchus contortus in Gerbils and Relative Expression of Hc29 Gene in Transitional Larvae (L3–L4)

Acta Parasitologica ◽

10.1007/s11686-021-00364-w ◽

2021 ◽

Author(s):

Rosa Isabel Higuera-Piedrahita ◽

Mariana Dolores-Hernández ◽

Luis Gerardo Jiménez-Pérez ◽

Brígida C. Camacho-Enríquez ◽

Alejandro Zamilpa ◽

...

Keyword(s):

Haemonchus Contortus ◽

Anthelmintic Activity ◽

Relative Expression

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Anthelmintic activity in vitro of Citrus sinensis and Melaleuca quinquenervia essential oil from Cuba on Haemonchus contortus

Industrial Crops and Products ◽

10.1016/j.indcrop.2015.07.056 ◽

2015 ◽

Vol 76 ◽

pp. 647-652 ◽

Cited By ~ 7

Author(s):

Yousmel Aleman Gaínza ◽

Luciana Ferreira Domingues ◽

Oriela Pino Perez ◽

Márcio Dias Rabelo ◽

Eugenio Roque López ◽

...

Keyword(s):

Essential Oil ◽

Haemonchus Contortus ◽

Citrus Sinensis ◽

Anthelmintic Activity ◽

Melaleuca Quinquenervia

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The effects of flubendazole and its metabolites on the larval development of Haemonchus contortus (Nematoda: Trichostrongylidae): an in vitro study

Helminthologia ◽

10.2478/s11687-010-0042-7 ◽

2010 ◽

Vol 47 (4) ◽

pp. 269-272 ◽

Cited By ~ 6

Author(s):

H. Bártíková ◽

L. Skálová ◽

J. Lamka ◽

B. Szotáková ◽

M. Várady

Keyword(s):

Larval Development ◽

Haemonchus Contortus ◽

Anthelmintic Activity ◽

In Vitro Study ◽

Lethal Concentration ◽

Resistant Isolate ◽

Susceptible Isolate ◽

Marked Resistance ◽

Larval Development Test

AbstractThe anthelmintic effects of flubendazole (FLU), its two main metabolites reduced flubendazole (FLU-R) and hydrolyzed flubendazole (FLU-H), and thiabendazole (TBZ) were compared using an in vitro larval development test in two isolates of Haemonchus contortus, a fully susceptible isolate (HCS) and a multi-resistant isolate (HCR). Results were quantified as 50 % lethal concentration (LC50), 99 % lethal concentration (LC99), efficacy factor (EF), and resistance factor (RF). For HCS, both LC50 and LC99 of FLU were lower than those of the reference TBZ. The anthelmintic activity of FLU-R in HCS and HCR was 13 and 6 times lower than the activity of FLU, respectively. The anthelmintic activity of FLU-H was negligible (approximately 363–853 times lower) compared to that of FLU. Although a marked resistance of the HCR isolate to TBZ was confirmed, only a low tolerance to FLU-R and slightly higher tolerance to FLU were found.

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