Prosopis juliflora pods alkaloid-rich fraction: In vitro anthelmintic activity on goat gastrointestinal parasites and its cytotoxicity on vero cells

Phytotherapy may be an alternative strategy for controlling gastrointestinal parasites. This study evaluated the anthelmintic efficacy of Eucalyptus citriodora essential oil (EcEO). The in vitro effects of EcEO were determined through testing the inhibition of egg hatching and larval development of Haemonchus contortus. EcEO was subjected to acute toxicity testing on mice, orally and intraperitoneally. The in vivo effects of EcEO were determined by the fecal egg count reduction test (FECRT) in goats infected with gastrointestinal nematodes. The results showed that 5.3 mg.mL-1 EcEO inhibited egg hatching by 98.8% and 10.6 mg.mL-1 EcEO inhibited H. contortus larval development by 99.71%. The lethal doses for 50% of the mice were 4153 and 622.8 mg.kg-1, for acute toxicity orally and intraperitoneally. In the FECRT, the efficacy of EcEO and ivermectin was 66.25 and 79.16% respectively, on goat gastrointestinal nematodes eight days after treatment. EcEO showed in vitro and in vivo anthelmintic activity.

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Ovicidal activity of succinic acid isolated from sisal waste (Agave sisalana) against gastrointestinal nematodes of goats

Ciência Rural ◽

10.1590/0103-8478cr20170036 ◽

2017 ◽

Vol 47 (8) ◽

Cited By ~ 1

Author(s):

Nathália Silva de Souza Santos ◽

Jener David Gonçalves Santos ◽

Francianne Oliveira Santos ◽

Taiane Menezes Serra ◽

Hélimar Gonçalves de Lima ◽

...

Keyword(s):

Succinic Acid ◽

Anthelmintic Activity ◽

Vero Cells ◽

Gastrointestinal Nematodes ◽

Significant Inhibition ◽

Egg Hatching ◽

The Mean ◽

Agave Sisalana

ABSTRACT: This study was conducted to evaluate the in vitro anthelmintic activity of the succinic acid (SA) isolated from sisal waste against gastrointestinal nematodes of goats, using the egg hatching and larvae motility assays. In addition, potential cytotoxicity of SA on Vero cell cultures was investigated by means of MTT (3-4,5-dimethylthiazol-2-yl, 2,5diphenyltetrazolium bromide) test. The SA induced a significant inhibition of egg hatching (P<0.05) at all concentrations tested (60 to 250µg mL-1), and the concentrations to inhibit 50% (EC50) and 90% (EC90) values (mean ± standard deviation) were 90.3±2.8 and 130.6±3.5µg mL-1, respectively. The SA has not shown larvicidal activity. The SA was less toxic to the Vero cells, with the mean percentage of cell viability equal to 85±6.2% at the concentration of 130µg mL-1. The results suggested that SA has potential anthelmintic effect; although, more research is needed to confirm its activity in vivo.

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In vitro effects of Coriandrum sativum, Tagetes minuta, Alpinia zerumbet and Lantana camara essential oils on Haemonchus contortus

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612013000400004 ◽

2013 ◽

Vol 22 (4) ◽

pp. 463-469 ◽

Cited By ~ 10

Author(s):

Iara Tersia Freitas Macedo ◽

Lorena Mayana Beserra de Oliveira ◽

Ana Lourdes Fernandes Camurça-Vasconcelos ◽

Wesley Lyeverton Correia Ribeiro ◽

Jessica Maria Leite dos Santos ◽

...

Keyword(s):

Essential Oils ◽

Larval Development ◽

Haemonchus Contortus ◽

Small Ruminants ◽

Anthelmintic Activity ◽

Gastrointestinal Parasites ◽

Coriandrum Sativum ◽

Tagetes Minuta ◽

Alpinia Zerumbet

Phytotherapy can be an alternative for the control of gastrointestinal parasites of small ruminants. This study evaluated the efficacy of Alpinia zerumbet, Coriandrum sativum, Tagetes minuta and Lantana camaraessential oils by two in vitro assays on Haemonchus contortus, an egg hatch test (EHT) and larval development test (LDT). No effect was observed for L. camara in the EHT. A. zerumbet, C. sativum and T. minuta essential oils exhibited a dose-dependent effect in the EHT, inhibiting 81.2, 99 and 98.1% of H. contortus larvae hatching, respectively, at a concentration of 2.5 mg mL-1. The effective concentration to inhibit 50% (EC50) of egg hatching was 0.94, 0.63 and 0.53 mg mL-1 for A. zerumbet, C. sativum and T. minuta essential oils, respectively. In LDT, L. camara, A. zerumbet, C. sativum and T. minuta at concentration of 10 mg mL-1 inhibited 54.9, 94.2, 97.8 and 99.5% of H. contortus larval development, presenting EC50 values of 6.32, 3.88, 2.89 and 1.67 mg mL-1, respectively. Based on the promising results presented in this in vitro model, it may be possible use of these essential oils to control gastrointestinal nematodes. However, their anthelmintic activity should be confirmed in vivo.

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Hydrolyzable tannins fighting gastrointestinal parasites of ruminants: chemical structure predicts in vitro anthelmintic activity

Planta Medica ◽

10.1055/s-0036-1596980 ◽

2016 ◽

Vol 81 (S 01) ◽

pp. S1-S381

Author(s):

MT Engström ◽

M Karonen ◽

JR Ahern ◽

N Baert ◽

B Payré ◽

...

Keyword(s):

Chemical Structure ◽

Anthelmintic Activity ◽

Gastrointestinal Parasites ◽

Hydrolyzable Tannins

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In vitro anthelmintic activity of the Zizyphus joazeiro bark against gastrointestinal nematodes of goats and its cytotoxicity on Vero cells

Veterinary Parasitology ◽

10.1016/j.vetpar.2016.06.004 ◽

2016 ◽

Vol 226 ◽

pp. 10-16 ◽

Cited By ~ 11

Author(s):

Danilo Cavalcanti Gomes ◽

Hélimar Gonçalves de Lima ◽

Ariádne Vieira Vaz ◽

Nathália Silva Santos ◽

Francianne Oliveira Santos ◽

...

Keyword(s):

Anthelmintic Activity ◽

Vero Cells ◽

Gastrointestinal Nematodes

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In vitro anthelmintic activity of Pothomorphe umbellata (L.) Miq. (Piperaceae) against gastrointestinal parasites from sheep

BMC Proceedings ◽

10.1186/1753-6561-8-s4-p155 ◽

2014 ◽

Vol 8 (S4) ◽

Cited By ~ 1

Author(s):

Luis Eduardo Ferreira ◽

Pedro Castro ◽

Franca Suzelei ◽

Beleboni Rene

Keyword(s):

Anthelmintic Activity ◽

Gastrointestinal Parasites

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In vitro anthelmintic activity of Pimienta dioica and Origanum vulgare essential oils on gastrointestinal nematodes from sheep and cattle

Journal of Parasitic Diseases ◽

10.1007/s12639-021-01401-1 ◽

2021 ◽

Author(s):

Gerardo Jiménez-Penago ◽

Roberto González-Garduño ◽

Luciano Martínez-Bolaños ◽

Ema Maldonado-Siman ◽

Alvar A. Cruz-Tamayo ◽

...

Keyword(s):

Essential Oils ◽

Anthelmintic Activity ◽

Gastrointestinal Nematodes ◽

Origanum Vulgare

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Chloroquine and Sulfadoxine Derivatives Inhibit ZIKV Replication in Cervical Cells

Viruses ◽

10.3390/v13010036 ◽

2020 ◽

Vol 13 (1) ◽

pp. 36

Author(s):

Audrien Alves Andrade de Souza ◽

Lauana Ribas Torres ◽

Lyana Rodrigues Pinto Lima Capobianco ◽

Vanessa Salete de Paula ◽

Cynthia Machado Cascabulho ◽

...

Keyword(s):

Antiviral Activity ◽

Specific Treatment ◽

Vero Cells ◽

Vehicle Control ◽

Therapeutic Window ◽

Chemical Structures ◽

Hybrid Compounds ◽

Cervical Cells ◽

Zika Fever

Despite the severe morbidity caused by Zika fever, its specific treatment is still a challenge for public health. Several research groups have investigated the drug repurposing of chloroquine. However, the highly toxic side effect induced by chloroquine paves the way for the improvement of this drug for use in Zika fever clinics. Our aim is to evaluate the anti-Zika virus (ZIKV) effect of hybrid compounds derived from chloroquine and sulfadoxine antimalarial drugs. The antiviral activity of hybrid compounds (C-Sd1 to C-Sd7) was assessed in an in-vitro model of human cervical and Vero cell lines infected with a Brazilian (BR) ZIKV strain. First, we evaluated the cytotoxic effect on cultures treated with up to 200 µM of C-Sds and observed CC50 values that ranged from 112.0 ± 1.8 to >200 µM in cervical cells and 43.2 ± 0.4 to 143.0 ± 1.3 µM in Vero cells. Then, the cultures were ZIKV-infected and treated with up to 25 µM of C-Sds for 48 h. The treatment of cervical cells with C-Sds at 12 µM induced a reduction of 79.8% ± 4.2% to 90.7% ± 1.5% of ZIKV–envelope glycoprotein expression in infected cells as compared to 36.8% ± 2.9% of infection in vehicle control. The viral load was also investigated and revealed a reduction of 2- to 3-logs of ZIKV genome copies/mL in culture supernatants compared to 6.7 ± 0.7 × 108 copies/mL in vehicle control. The dose–response curve by plaque-forming reduction (PFR) in cervical cells revealed a potent dose-dependent activity of C-Sds in inhibiting ZIKV replication, with PFR above 50% and 90% at 6 and 12 µM, respectively, while 25 µM inhibited 100% of viral progeny. The treatment of Vero cells at 12 µM led to 100% PFR, confirming the C-Sds activity in another cell type. Regarding effective concentration in cervical cells, the EC50 values ranged from 3.2 ± 0.1 to 5.0 ± 0.2 µM, and the EC90 values ranged from 7.2 ± 0.1 to 11.6 ± 0.1 µM, with selectivity index above 40 for most C-Sds, showing a good therapeutic window. Here, our aim is to investigate the anti-ZIKV activity of new hybrid compounds that show highly potent efficacy as inhibitors of ZIKV in-vitro infection. However, further studies will be needed to investigate whether these new chemical structures can lead to the improvement of chloroquine antiviral activity.

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