scholarly journals Lulo cell line derived from Lutzomyia longipalpis (Diptera: Psychodidae): a novel model to assay Leishmania spp. and vector interaction

2011 ◽  
Vol 4 (1) ◽  
pp. 216 ◽  
Author(s):  
Luzia MC Côrtes ◽  
Roger MM Silva ◽  
Bernardo AS Pereira ◽  
Camila Guerra ◽  
Angela C Zapata ◽  
...  
Keyword(s):  
Cell Line ◽  
Lutzomyia Longipalpis ◽  
Vector Interaction ◽  
Leishmania Spp ◽  
Novel Model ◽  
Lulo Cell

scholarly journals Insights into the proteomic profile and gene expression of Lutzomyia longipalpis-derived Lulo cell line

2020 ◽  
Vol 115 ◽  
Author(s):  
Luzia Monteiro de Castro Côrtes ◽  
Daniela de Pita-Pereira ◽  
Priscila Silva Grijó Farani ◽  
Bernardo Acácio Santini Pereira ◽  
Geovane Dias-Lopes ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Lutzomyia Longipalpis ◽  
Proteomic Profile ◽  
Lulo Cell

scholarly journals Microbiota do trato digestivo de fêmeas de Lutzomyia longipalpis (Lutz & Neiva, 1912) (Diptera: Psychodidae) provenientes de colônia alimentadas com sangue e com sangue e sacarose

2001 ◽  
Vol 17 (1) ◽  
pp. 229-232 ◽  
Author(s):  
Sandra Maria Pereira de Oliveira ◽  
Bianca Aguiar de Morais ◽  
Claudia Abrantes Gonçalves ◽  
Cristina Maria Giordano-Dias ◽  
Maurício Luiz Vilela ◽  
...  
Keyword(s):  
Lutzomyia Longipalpis ◽  
Leishmania Spp

Há poucos estudos sobre a microbiota do trato digestivo de flebotomíneos, considerando-se que o sangue não é o único alimento ingerido. Os flebotomíneos, tanto os machos como as fêmeas, alimentam-se de açúcares, provenientes de várias fontes, possibilitando a ingestão de microrganismos. As chances de contaminação aumentam nos insetos criados em laboratório e pode interferir no desenvolvimento da Leishmania spp. Foi separado um total de 300 fêmeas, divididas em dois lotes, das quais extraímos o trato digestivo. No lote 1(fêmeas alimentadas com sangue e sacarose) das 10 espécies bacterianas isoladas, a família Enterobacteriaceae esteve representada pelos gêneros Serratia, Enterobacter e Yokenella, e o grupo dos não fermentadores pelos gêneros Pseudomonas, Acinetobacter e Stenotrophomonas. No lote 2 (fêmeas alimentadas apenas com sangue) das 8 espécies isoladas o grupo dos não fermentadores esteve representado pelos gêneros Acinetobacter, Stenotrophomonas, Burkolderia e Pseudomonas, e a família Enterobacteriaceae, pelos gêneros Enterobacter e Serratia.

The finding of Lutzomyia almerioi and Lutzomyia longipalpis naturally infected by Leishmania spp. in a cutaneous and canine visceral leishmaniases focus in Serra da Bodoquena, Brazil

2009 ◽  
Vol 160 (1-2) ◽  
pp. 18-24 ◽  
Author(s):  
Elisa San Martin Mouriz Savani ◽  
Vânia Lúcia Brandão Nunes ◽  
Eunice Aparecida Bianchi Galati ◽  
Tiago Moreno Castilho ◽  
Ricardo Andrade Zampieri ◽  
...  
Keyword(s):  
Lutzomyia Longipalpis ◽  
Leishmania Spp

scholarly journals The UOK 257 cell line: a novel model for studies of the human Birt–Hogg–Dubé gene pathway

2008 ◽  
Vol 180 (2) ◽  
pp. 100-109 ◽  
Author(s):  
Youfeng Yang ◽  
Hesed M. Padilla-Nash ◽  
Manish A. Vira ◽  
Mones S. Abu-Asab ◽  
Daniel Val ◽  
...  
Keyword(s):  
Cell Line ◽  
Gene Pathway ◽  
Novel Model

scholarly journals Assessment of PCR in the detection of Leishmania spp in experimentally infected individual phlebotomine sandflies (Diptera: Psychodidae: Phlebotominae)

2002 ◽  
Vol 44 (5) ◽  
pp. 255-259 ◽  
Author(s):  
Érika M. MICHALSKY ◽  
Consuelo L. FORTES-DIAS ◽  
Paulo F.P. PIMENTA ◽  
Nágila F.C. SECUNDINO ◽  
Edelberto S. DIAS
Keyword(s):  
Digestive Tract ◽  
Infected Individual ◽  
Dna Amplification ◽  
Leishmania Braziliensis ◽  
Lutzomyia Longipalpis ◽  
Leishmania Chagasi ◽  
Phlebotomine Sandflies ◽  
Leishmania Spp ◽  
Polymerase Chain ◽  
Lutzomyia Migonei

DNA amplification by the polymerase chain reaction (PCR) was applied in the investigation of the presence of Leishmania (Kinetoplastida: Trypanosomatidae) parasites in single phlebotomine sandflies. Three phlebotomine/parasite pairs were used: Lutzomyia longipalpis/Leishmania chagasi, Lutzomyia migonei/Leishmania amazonensis and Lutzomyia migonei/Leishmania braziliensis, all of them incriminated in the transmission of visceral or cutaneous leishmaniasis. DNA extraction was performed with whole insects, with no need of previous digestive tract dissection or pooling specimens. The presence of either mouse blood in the digestive tract of the sandflies or the digestive tract itself did not interfere in the PCR. Infection by as few as 10 Leishmania sp. per individual were sufficient for DNA amplification with genus-specific primers. Using primers for L. braziliensis and L. mexicana complexes, respectively, it was possible to discriminate between L. braziliensis and L. amazonensis in experimentally infected vectors (L. migonei).

scholarly journals Molecular and parasitological detection ofLeishmania spp. in a dipteran of the speciesTabanus importunus

2013 ◽  
Vol 22 (4) ◽  
pp. 605-607 ◽  
Author(s):  
Willian Marinho Dourado Coelho ◽  
Katia Denise Saraiva Bresciani
Keyword(s):  
Visceral Leishmaniasis ◽  
Deoxyribonucleic Acid ◽  
Molecular Detection ◽  
Lutzomyia Longipalpis ◽  
Sand Flies ◽  
Thoracic Region ◽  
Main Species ◽  
Leishmania Spp ◽  
Genus Leishmania ◽  
Horse Fly

Leishmaniasis is an important chronic zoonosis caused by protozoa of the genus Leishmania spp. The major vectors of this protozoosis are sand flies, and Lutzomyia longipalpis is considered the main species implicated in the transmission of American Visceral Leishmaniasis in Brazil. The presence of the parasite's deoxyribonucleic acid (DNA) in ectoparasites such as ticks and fleas has prompted speculations about the existence of new vectors in the cycle of leishmaniasis. The aim of this paper is to report the molecular detection of Leishmania spp. in a horse fly of the species Tabanus importunus which parasitized an oligosymptomatic dog infected with Leishmania spp. Molecular amplification of the protozoan's DNA in the head, thoracic region and abdomen of the tabanid tested positive for Leishmania complex. This is the first report of the presence of DNA from Leishmaniaspp. in dipterous insects of the species T. importunus.

scholarly journals Experimental infection of Leishmania (L.) chagasi in a cell line derived from Lutzomyia longipalpis (Diptera:Psychodidae)

2005 ◽  
Vol 100 (6) ◽  
pp. 519-525 ◽  
Author(s):  
Felio J Bello ◽  
Astrid J Mejía ◽  
María del Pilar Corena ◽  
Martha Ayala ◽  
Ladys Sarmiento ◽  
...  
Keyword(s):  
Cell Line ◽  
Experimental Infection ◽  
Lutzomyia Longipalpis ◽  
A Cell

scholarly journals Lutzomyia longipalpis naturally infected by Leishmania (L.) chagasi in Várzea Grande, Mato Grosso State, Brazil, an area of intense transmission of visceral leishmaniasis

2010 ◽  
Vol 26 (12) ◽  
pp. 2414-2419 ◽  
Author(s):  
Nanci A. Missawa ◽  
Érika Monteiro Michalsky ◽  
Consuelo Latorre Fortes-Dias ◽  
Edelberto Santos Dias
Keyword(s):  
Visceral Leishmaniasis ◽  
Polymerase Chain Reaction Amplification ◽  
Natural Infection ◽  
Rflp Analysis ◽  
Lutzomyia Longipalpis ◽  
Sand Flies ◽  
Mato Grosso ◽  
Phlebotomine Sand Flies ◽  
Leishmania Spp ◽  
Reaction Amplification

The American visceral leishmaniasis (AVL) is caused by parasites belonging to the genus Leishmania (Trypanosomatidae) and is transmitted to humans through the bite of certain species of infected phlebotomine sand flies. In this study, we investigated the natural infection ratio of Lutzomyia longipalpis, the main vector species of AVL in Brazil, in Várzea Grande, Mato Grosso State. Between July 2004 and June 2006, phlebotomine sand flies were captured in peridomestic areas using CDC light-traps. Four hundred and twenty (420) specimens of Lu. longipalpis were captured. 42 pools, containing 10 specimens of Lu. longipalpis each, were used for genomic DNA extraction and PCR (polymerase chain reaction) amplification. Leishmania spp. DNA was detected in three out of the 42 pools tested, resulting in a minimal infection ratio of 0.71%. Restriction fragment length polymorphism (RFLP) analysis indicated that Leishmania (L.) chagasi was the infective agent in the positive pools.

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