scholarly journals Microbiota do trato digestivo de fêmeas de Lutzomyia longipalpis (Lutz & Neiva, 1912) (Diptera: Psychodidae) provenientes de colônia alimentadas com sangue e com sangue e sacarose

2001 ◽  
Vol 17 (1) ◽  
pp. 229-232 ◽  
Author(s):  
Sandra Maria Pereira de Oliveira ◽  
Bianca Aguiar de Morais ◽  
Claudia Abrantes Gonçalves ◽  
Cristina Maria Giordano-Dias ◽  
Maurício Luiz Vilela ◽  
...  
Keyword(s):  
Lutzomyia Longipalpis ◽  
Leishmania Spp

Há poucos estudos sobre a microbiota do trato digestivo de flebotomíneos, considerando-se que o sangue não é o único alimento ingerido. Os flebotomíneos, tanto os machos como as fêmeas, alimentam-se de açúcares, provenientes de várias fontes, possibilitando a ingestão de microrganismos. As chances de contaminação aumentam nos insetos criados em laboratório e pode interferir no desenvolvimento da Leishmania spp. Foi separado um total de 300 fêmeas, divididas em dois lotes, das quais extraímos o trato digestivo. No lote 1(fêmeas alimentadas com sangue e sacarose) das 10 espécies bacterianas isoladas, a família Enterobacteriaceae esteve representada pelos gêneros Serratia, Enterobacter e Yokenella, e o grupo dos não fermentadores pelos gêneros Pseudomonas, Acinetobacter e Stenotrophomonas. No lote 2 (fêmeas alimentadas apenas com sangue) das 8 espécies isoladas o grupo dos não fermentadores esteve representado pelos gêneros Acinetobacter, Stenotrophomonas, Burkolderia e Pseudomonas, e a família Enterobacteriaceae, pelos gêneros Enterobacter e Serratia.

The finding of Lutzomyia almerioi and Lutzomyia longipalpis naturally infected by Leishmania spp. in a cutaneous and canine visceral leishmaniases focus in Serra da Bodoquena, Brazil

2009 ◽  
Vol 160 (1-2) ◽  
pp. 18-24 ◽  
Author(s):  
Elisa San Martin Mouriz Savani ◽  
Vânia Lúcia Brandão Nunes ◽  
Eunice Aparecida Bianchi Galati ◽  
Tiago Moreno Castilho ◽  
Ricardo Andrade Zampieri ◽  
...  
Keyword(s):  
Lutzomyia Longipalpis ◽  
Leishmania Spp

scholarly journals Assessment of PCR in the detection of Leishmania spp in experimentally infected individual phlebotomine sandflies (Diptera: Psychodidae: Phlebotominae)

2002 ◽  
Vol 44 (5) ◽  
pp. 255-259 ◽  
Author(s):  
Érika M. MICHALSKY ◽  
Consuelo L. FORTES-DIAS ◽  
Paulo F.P. PIMENTA ◽  
Nágila F.C. SECUNDINO ◽  
Edelberto S. DIAS
Keyword(s):  
Digestive Tract ◽  
Infected Individual ◽  
Dna Amplification ◽  
Leishmania Braziliensis ◽  
Lutzomyia Longipalpis ◽  
Leishmania Chagasi ◽  
Phlebotomine Sandflies ◽  
Leishmania Spp ◽  
Polymerase Chain ◽  
Lutzomyia Migonei

DNA amplification by the polymerase chain reaction (PCR) was applied in the investigation of the presence of Leishmania (Kinetoplastida: Trypanosomatidae) parasites in single phlebotomine sandflies. Three phlebotomine/parasite pairs were used: Lutzomyia longipalpis/Leishmania chagasi, Lutzomyia migonei/Leishmania amazonensis and Lutzomyia migonei/Leishmania braziliensis, all of them incriminated in the transmission of visceral or cutaneous leishmaniasis. DNA extraction was performed with whole insects, with no need of previous digestive tract dissection or pooling specimens. The presence of either mouse blood in the digestive tract of the sandflies or the digestive tract itself did not interfere in the PCR. Infection by as few as 10 Leishmania sp. per individual were sufficient for DNA amplification with genus-specific primers. Using primers for L. braziliensis and L. mexicana complexes, respectively, it was possible to discriminate between L. braziliensis and L. amazonensis in experimentally infected vectors (L. migonei).

scholarly journals Molecular and parasitological detection ofLeishmania spp. in a dipteran of the speciesTabanus importunus

2013 ◽  
Vol 22 (4) ◽  
pp. 605-607 ◽  
Author(s):  
Willian Marinho Dourado Coelho ◽  
Katia Denise Saraiva Bresciani
Keyword(s):  
Visceral Leishmaniasis ◽  
Deoxyribonucleic Acid ◽  
Molecular Detection ◽  
Lutzomyia Longipalpis ◽  
Sand Flies ◽  
Thoracic Region ◽  
Main Species ◽  
Leishmania Spp ◽  
Genus Leishmania ◽  
Horse Fly

Leishmaniasis is an important chronic zoonosis caused by protozoa of the genus Leishmania spp. The major vectors of this protozoosis are sand flies, and Lutzomyia longipalpis is considered the main species implicated in the transmission of American Visceral Leishmaniasis in Brazil. The presence of the parasite's deoxyribonucleic acid (DNA) in ectoparasites such as ticks and fleas has prompted speculations about the existence of new vectors in the cycle of leishmaniasis. The aim of this paper is to report the molecular detection of Leishmania spp. in a horse fly of the species Tabanus importunus which parasitized an oligosymptomatic dog infected with Leishmania spp. Molecular amplification of the protozoan's DNA in the head, thoracic region and abdomen of the tabanid tested positive for Leishmania complex. This is the first report of the presence of DNA from Leishmaniaspp. in dipterous insects of the species T. importunus.

scholarly journals Lutzomyia longipalpis naturally infected by Leishmania (L.) chagasi in Várzea Grande, Mato Grosso State, Brazil, an area of intense transmission of visceral leishmaniasis

2010 ◽  
Vol 26 (12) ◽  
pp. 2414-2419 ◽  
Author(s):  
Nanci A. Missawa ◽  
Érika Monteiro Michalsky ◽  
Consuelo Latorre Fortes-Dias ◽  
Edelberto Santos Dias
Keyword(s):  
Visceral Leishmaniasis ◽  
Polymerase Chain Reaction Amplification ◽  
Natural Infection ◽  
Rflp Analysis ◽  
Lutzomyia Longipalpis ◽  
Sand Flies ◽  
Mato Grosso ◽  
Phlebotomine Sand Flies ◽  
Leishmania Spp ◽  
Reaction Amplification

The American visceral leishmaniasis (AVL) is caused by parasites belonging to the genus Leishmania (Trypanosomatidae) and is transmitted to humans through the bite of certain species of infected phlebotomine sand flies. In this study, we investigated the natural infection ratio of Lutzomyia longipalpis, the main vector species of AVL in Brazil, in Várzea Grande, Mato Grosso State. Between July 2004 and June 2006, phlebotomine sand flies were captured in peridomestic areas using CDC light-traps. Four hundred and twenty (420) specimens of Lu. longipalpis were captured. 42 pools, containing 10 specimens of Lu. longipalpis each, were used for genomic DNA extraction and PCR (polymerase chain reaction) amplification. Leishmania spp. DNA was detected in three out of the 42 pools tested, resulting in a minimal infection ratio of 0.71%. Restriction fragment length polymorphism (RFLP) analysis indicated that Leishmania (L.) chagasi was the infective agent in the positive pools.

scholarly journals Lulo cell line derived from Lutzomyia longipalpis (Diptera: Psychodidae): a novel model to assay Leishmania spp. and vector interaction

2011 ◽  
Vol 4 (1) ◽  
pp. 216 ◽  
Author(s):  
Luzia MC Côrtes ◽  
Roger MM Silva ◽  
Bernardo AS Pereira ◽  
Camila Guerra ◽  
Angela C Zapata ◽  
...  
Keyword(s):  
Cell Line ◽  
Lutzomyia Longipalpis ◽  
Vector Interaction ◽  
Leishmania Spp ◽  
Novel Model ◽  
Lulo Cell

scholarly journals Antibodies to Leishmania spp. in domestic felines

2015 ◽  
Vol 24 (4) ◽  
pp. 464-470 ◽  
Author(s):  
Gabriela Capriogli Oliveira ◽  
Laís Moraes Paiz ◽  
Benedito Donizete Menozzi ◽  
Michele de Souza Lima ◽  
Carla Cristina Guimarães de Moraes ◽  
...  
Keyword(s):  
Immunofluorescence Assay ◽  
Urban Environments ◽  
Lutzomyia Longipalpis ◽  
Insect Vector ◽  
Domestic Cats ◽  
Leishmania Spp ◽  
Infection Source ◽  
Vector Borne ◽  
Positive Results ◽  
Genus Leishmania

Abstract Leishmaniasis is a vector-borne zoonotic disease caused by protozoa in the genus Leishmania, typical of rural and peri-urban environments. The causative agent of American visceral leishmaniasis is Leishmania (Leishmania) infantum chagasi and the main insect vector in Brazil is Lutzomyia longipalpis. Dogs (Canis familiaris) are important in the transmission of the disease, as a reservoir closely related to humans and an infection source for phlebotomine vectors. Since 1990, an increasing number of feline leishmaniasis cases have been reported, suggesting that domestic cats (Felis catus) might be involved in the epidemiology of the disease. The present study analyzed the prevalence of anti-Leishmania spp. antibodies in naturally infected domestic cats from various neighborhoods in the municipality of Belém, Pará, Brazil, using the indirect immunofluorescence assay (IFA) and the direct agglutination test (DAT). Among the 443 samples tested, 18 (4.06%) presented positive reactions in the IFA. The observed titers were 40 IU in 4.97% of the samples and 80 IU in 0.90%. In the DAT test, positive results were found in 25 (5.64%) of the samples. The observed titers were also 40 IU (4.97%) and 80 IU (0.68%). The agreement rate between the two tests was considered low (Kappa coefficient = 0.10).

scholarly journals Molecular detection of Leishmania spp in Lutzomyia longipalpis in the city of Lavras, Minas Gerais, Brazil

2019 ◽  
Vol 52 (9) ◽  
Author(s):  
J.C. Castro ◽  
L.L. Bueno ◽  
T.F. Milagres ◽  
F.D. Rêgo ◽  
C.M.F. Gontijo ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Minas Gerais ◽  
Lutzomyia Longipalpis ◽  
Leishmania Spp ◽  
The City

Maintenance and Productivity of a Lutzomyia longipalpis (Diptera: Psychodidae) Colony from an Area Endemic for Visceral and Cutaneous Leishmaniasis in Northeastern Brazil

2021 ◽  
Author(s):  
Rosa Cristina Ribeiro da Silva ◽  
Léo Nava Piorsky Dominici Cruz ◽  
João Manoel da Silva Coutinho ◽  
Núrzia Cristina Correia Santana ◽  
José Manuel Macário Rebêlo
Keyword(s):  
Vector Competence ◽  
Natural Populations ◽  
Northeastern Brazil ◽  
Sand Fly ◽  
Lutzomyia Longipalpis ◽  
Infection Rates ◽  
Laboratory Conditions ◽  
Leishmania Spp ◽  
Cutaneous Form ◽  
Males And Females

Abstract Studies on experimental sand fly infection require the availability of colonies and laboratory conditions. In Brazil, Lutzomyia longipalpis (Diptera: Psychodidae) (Lutz and Neiva 1912) is responsible for the highest infection rates by Leishmania spp. and this species is one of the most suitable species for laboratory colonization. In this study, we describe a method for growing Lu. longipalpis in laboratory conditions (10 generations) from natural populations sampled from a region of high endemicity for visceral leishmaniasis in the state of Maranhão, Northeastern Brazil. Using two methods (individualized or grouped females), the colony’s highest productivity occurred in the first four generations, where all stages presented with high frequency. Nonviable eggs represented more than 50% of the total eggs produced by engorged females, while pupae were more resistant to fungal contamination, with a mortality rate of only 2%. In both methods, there was a predominance of female emergence; however, the ratio between males and females did not show significant differences, IF (P = 0.8023) and GF (P = 0.1831). Using the method of individualized females, the F4 generation took the longest to appear (234 d; 64 ± 57 d); by grouped females, F3 took the longest to appear (102 d; 47 ± 20 d). This method provides sufficient numbers of insects to perform vector competence tests for Leishmania spp. that cause the cutaneous form of leishmaniasis, usually found in Lu. longipalpis sampled from the study location.

scholarly journals Eco-epidemiological study on sandflies and environmental aspects related to the transmission of leishmaniasis in a municipality of Minas Gerais, Brazil, 2015-2016

2019 ◽  
Vol 71 (6) ◽  
pp. 1805-1814
Author(s):  
L.B. Gomes ◽  
E.S. Dias ◽  
S.C.P.F. Silva ◽  
P.C.F.B. Carvalho ◽  
A.G.R.C. Santos ◽  
...  
Keyword(s):  
Spatial Analysis ◽  
Epidemiological Study ◽  
Lutzomyia Longipalpis ◽  
Spearman Correlation ◽  
Light Traps ◽  
Environmental Aspects ◽  
Leishmania Spp ◽  
Fuzzy Logic Method ◽  
Climatic Characteristics ◽  
Systematic Collection

ABSTRACT The aim of this study was to evaluate Leishmania vectors and investigate the environmental and climatic characteristics related to their transmission. This was a prospective eco-epidemiological study involving the systematic collection of sandflies and a canine serological survey in areas of municipality in Brazil. Blood samples from 1,752 dogs were examined to test for canine visceral leishmaniasis (VL). An entomological evaluation was carried out using 24 light traps in the peridomicile and intradomicile areas of 12 households for 12 months. Climate variables were analyzed using a simple Spearman correlation and a spatial analysis via the fuzzy logic method and K function. The prevalence rate of canine VL was 4.1% and 7.1% in the two areas that were studied, respectively. The entomological study resulted in the capture of 431 sandflies. Most (74%) of the specimens were captured in the peridomiciliary area, and 5.6% of the samples that were analyzed using molecular biology were positive for Leishmania spp. In particular, Leishmania infantum was found in 100% of the positive samples. The spatial analysis showed that one particular area presented a higher concentration of high-density overlapping points of Lutzomyia longipalpis and seropositive dog populations, indicating a higher risk of the concomitant occurrence of both events. The results show that a parasite-reservoir-vector interface is active in the studied areas.

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